塔拉多糖的脱色工艺化研究
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摘要
塔拉多糖是一种半乳甘露聚糖胶,对于我们具有非常重要的应用价值。本实验主要对塔拉提取物中的塔拉多糖进行脱色工艺化研究;在单因素实验的基础上,对活性炭颗粒质量、脱色时间、脱色温度以及脱色次数这四种因素进行正交优化实验,其最佳脱色实验参数为:活性炭颗粒0.6 g,脱色45 min,脱色温度45℃脱色次数3次,脱色率可以达到50.21%,同时多糖类保留率为90.39%。
Tara polysaccharide is a kind of galactose,which has a very important application value for us. We mainly studies the tara polysaccharide extract from tara seeds. By the single factor experiment,we optimized four factors including the activated carbon particle quality,decolorization time,decolorization temperature and decolorization times. The best optimum conditions were under activated carbon particles of 0. 6 g,decolorization time of 45 min,decolorization temperature of 45℃,decolorization times of 3,the decolorization rate can be achieved 50. 21%,at the same time,and the retention rate of polysaccharide was 90. 39%.
Tara polysaccharide is a kind of galactose,which has a very important application value for us. We mainly studies the tara polysaccharide extract from tara seeds. By the single factor experiment,we optimized four factors including the activated carbon particle quality,decolorization time,decolorization temperature and decolorization times. The best optimum conditions were under activated carbon particles of 0. 6 g,decolorization time of 45 min,decolorization temperature of 45℃,decolorization times of 3,the decolorization rate can be achieved 50. 21%,at the same time,and the retention rate of polysaccharide was 90. 39%.
引文
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3 .蒋建新,朱莉伟,张卫明,等.塔拉多糖胶的研究[J].西南林学院学报,2003,23(4):12-16.Jiang J X,Zhu L W,Zhang W M,et al.Study on properties of Caesalpinia spinosa polysaccharide gum[J].Journal of Southwest Forestry College,2003,23(4):12-16.
4 .夏定久,李志国.塔拉的工业用途与丰产栽培[M].昆明:云南科技出版社,2005:175-179.Xia D J,Li Z G.Industrial use and high yield of Tara[M].Kunming:Yunnan Science&Technology Press,2005:175-179.
5 .陈笳鸿,吴在嵩,毕良武,等.塔拉提取物化学利用的研究进展[J].林产化学与工业,1996,16(3):79-86.Chen J H,Wu Z S,Bi L W,et al.Development of research on chemical utilization of tara extractives[J].Chemistry and Industry of Forest Products,1996,16(3):79-86.
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8 .方婷,曾红亮,陈晓垚,等.猪肚菇粗多糖脱蛋白脱色工艺优化研究[J].热带作物学报,2015,36(6):1166-1172.Fang T,Zeng H L,Chen X Y,et al.Process optimization of deproteinization and decoloration from pork tripe mushroom crude polysaccharide[J].Chinese Journal of Tropical Crops,2015,36(6):1166-1172.
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11 .Palanivel R,Koshy Mathews P.Prediction and optimization of process parameter of friction stir welded AA5083-H111aluminum alloy using response surface methodology[J].Journal of Central South University,2012,19(1):1-8.
12 .Nassourou M A,Njintang Y N,NoubissiéT J B,et al.Genetics of seed flavonoid content and antioxidant activity in cowpea(Vigna unguiculata L.Walp.)[J].The Crop Journal,2016,4(5):391-397.
13 .孟宪军,李冬男,汪艳群,等.五味子多糖超声波提取条件的研究[J].食品工业科技,2010,31(4):313-315,319.Meng X J,Li D N,Wang Y Q,et al.Study on ultrasoundassisted extraction polysaccharides from Schisandra chinensis Turcz.Bail.[J].Science and Technology of Food Industry,2010,31(4):313-315,319.
14 .邸明磊,单晓菊,马丽娜,等.苯酚—硫酸法测定苦豆子多糖含量[J].现代中药研究与实践,2012,26(1):59-61.Di M L,Shan X J,Ma L N,et al.Determination of polysaccharide in Sophora alopecuroides L.by the method of phenol-vitriol[J].Research and Practice on Chinese Medicines,2012,26(1):59-61.
15 .谢建华,申明月,刘昕,等.苦瓜中多糖含量测定方法的研究[J].中国食品添加剂,2009(6):209-213.Xie J H,Shen M Y,Liu X,et al.Study on the determination method of polysaccharide content in Momordica charantia L.[J].China Food Additives,2009(6):209-213.
16 .刘燕,陈小银,杨丽丽,等.响应面优化菊芋菊糖的提取工艺研究[J].植物研究,2016,36(4):627-633.Liu Y,Chen X Y,Yang L L,et al.Extraction process optimization of inulin from Helianthus tuberosus L.by response surface methodology[J].Bulletin of Botanical Research,2016,36(4):627-633.
17 .路祺,高悦,项凤影,等.牡丹种荚多糖提取工艺研究[J].植物研究,2015,35(1):154-157.Lu Q,Gao Y,Xiang F Y,et al.Polysaccharide extraction from peony seed pods by water bath[J].Bulletin of Botanical Research,2015,35(1):154-157.
18 .冯慎,陈爽,李妍,等.植物多糖的纯化工艺研究进展[J].天津化工,2010,24(6):5-8.Feng S,Chen S,Li Y,et al.Process of work in polysaccharide’s purification[J].Tianjin Chemical Industry,2010,24(6):5-8.
2 .马文秀,罗庆云,尚征贤,等.塔拉豆多糖研究[J].林产化学与工业,1999,19(4):29-34.Ma W X,Luo Q Y,Shang Z X,et al.Study on polysaccharide in Tara seed[J].Chemistry and Industry of Forest Products,1999,19(4):29-34.
3 .蒋建新,朱莉伟,张卫明,等.塔拉多糖胶的研究[J].西南林学院学报,2003,23(4):12-16.Jiang J X,Zhu L W,Zhang W M,et al.Study on properties of Caesalpinia spinosa polysaccharide gum[J].Journal of Southwest Forestry College,2003,23(4):12-16.
4 .夏定久,李志国.塔拉的工业用途与丰产栽培[M].昆明:云南科技出版社,2005:175-179.Xia D J,Li Z G.Industrial use and high yield of Tara[M].Kunming:Yunnan Science&Technology Press,2005:175-179.
5 .陈笳鸿,吴在嵩,毕良武,等.塔拉提取物化学利用的研究进展[J].林产化学与工业,1996,16(3):79-86.Chen J H,Wu Z S,Bi L W,et al.Development of research on chemical utilization of tara extractives[J].Chemistry and Industry of Forest Products,1996,16(3):79-86.
6 .孟凡欣,蒋朝军,于笑坤,等.刺五加多糖的提取及含量测定[J].食品工业科技,2005,(7):110-112.Meng F X,Jiang C J,Yu X K,et al.Extraction of polysaccharides from Acanthopanax senticosus and determination of polysaccharides content[J].Science and Technology of Food Industry,2005,(7):110-112.
7 .何先元,许晋芳,王秋霜,等.当归多糖的超声提取及含量测定[J].中国药业,2010,19(19):34-35.He X Y,Xu J F,Wang Q S,et al.Ultrasonic extraction and determination of polysaccharide in Radix angelicae sinensis[J].China Pharmaceuticals,2010,19(19):34-35.
8 .方婷,曾红亮,陈晓垚,等.猪肚菇粗多糖脱蛋白脱色工艺优化研究[J].热带作物学报,2015,36(6):1166-1172.Fang T,Zeng H L,Chen X Y,et al.Process optimization of deproteinization and decoloration from pork tripe mushroom crude polysaccharide[J].Chinese Journal of Tropical Crops,2015,36(6):1166-1172.
9 .Omeje K O,Eze S O O,Ozougwu V E O,et al.Application of papain from Paw Paw(Carica papaya)latex in the Hydrolysis of Tiger Nut(C.esculentus)proteins[J].Mitteilungen Klosterneuburg,2014,64.
10 .Cao J,Gonzalez-Covarrubias V,Straubinger R M,et al.A rapid,reproducible,on-the-fly orthogonal array optimization method for targeted protein quantification by LC/MS and its application for accurate and sensitive quantification of carbonyl reductases in human liver[J].Analytical Chemistry,2010,82(7):2680-2689.
11 .Palanivel R,Koshy Mathews P.Prediction and optimization of process parameter of friction stir welded AA5083-H111aluminum alloy using response surface methodology[J].Journal of Central South University,2012,19(1):1-8.
12 .Nassourou M A,Njintang Y N,NoubissiéT J B,et al.Genetics of seed flavonoid content and antioxidant activity in cowpea(Vigna unguiculata L.Walp.)[J].The Crop Journal,2016,4(5):391-397.
13 .孟宪军,李冬男,汪艳群,等.五味子多糖超声波提取条件的研究[J].食品工业科技,2010,31(4):313-315,319.Meng X J,Li D N,Wang Y Q,et al.Study on ultrasoundassisted extraction polysaccharides from Schisandra chinensis Turcz.Bail.[J].Science and Technology of Food Industry,2010,31(4):313-315,319.
14 .邸明磊,单晓菊,马丽娜,等.苯酚—硫酸法测定苦豆子多糖含量[J].现代中药研究与实践,2012,26(1):59-61.Di M L,Shan X J,Ma L N,et al.Determination of polysaccharide in Sophora alopecuroides L.by the method of phenol-vitriol[J].Research and Practice on Chinese Medicines,2012,26(1):59-61.
15 .谢建华,申明月,刘昕,等.苦瓜中多糖含量测定方法的研究[J].中国食品添加剂,2009(6):209-213.Xie J H,Shen M Y,Liu X,et al.Study on the determination method of polysaccharide content in Momordica charantia L.[J].China Food Additives,2009(6):209-213.
16 .刘燕,陈小银,杨丽丽,等.响应面优化菊芋菊糖的提取工艺研究[J].植物研究,2016,36(4):627-633.Liu Y,Chen X Y,Yang L L,et al.Extraction process optimization of inulin from Helianthus tuberosus L.by response surface methodology[J].Bulletin of Botanical Research,2016,36(4):627-633.
17 .路祺,高悦,项凤影,等.牡丹种荚多糖提取工艺研究[J].植物研究,2015,35(1):154-157.Lu Q,Gao Y,Xiang F Y,et al.Polysaccharide extraction from peony seed pods by water bath[J].Bulletin of Botanical Research,2015,35(1):154-157.
18 .冯慎,陈爽,李妍,等.植物多糖的纯化工艺研究进展[J].天津化工,2010,24(6):5-8.Feng S,Chen S,Li Y,et al.Process of work in polysaccharide’s purification[J].Tianjin Chemical Industry,2010,24(6):5-8.