运输应激对拉布拉多犬血液皮质醇和生化指标的影响
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摘要
为探讨运输对犬的影响,本试验以拉布拉多犬为实验动物,运输前和运输2 h后分别采血,应用放射免疫、生化等方法测定血液皮质醇、促肾上腺皮质激素和生化指标。结果显示,运输后皮质醇(COR)浓度和谷草转氨酶(AST)比运输前显著增高(P<0.05),肌酸激酶(CK)活性极显著增高(P<0.01),促肾上腺皮质激素(ACTH)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、葡萄糖(GLU)无明显差异(P>0.05)。结果表明,2 h运输可引起拉布拉多犬血液皮质醇、谷草转氨酶和肌酸激酶水平的变化。
In order to study the effect of transport on dogs, Labrador retrievers were chosen and blood was collected before transport andafter 2-hour transport. Then serum cortisol, adrenocorticotropic hormone and biochemical indices were determined. The results showed: the concentration of cortisol and the activity of glutamic oxalacetic transaminase(AST) were significantly increased after transport(P<0.05). The activity of Creatine kinase(CK) was significantly increased(P<0.01). Adrenocorticotropic hormone(ACTH), alanine transaminase(ALT), alkaline phosphatase(ALP), lactate dehydrogenase(LDH), glucose(GLU) had no significant difference(P>0.05). In conclusion, 2-hour transport stress can induce the change of serum cortisol, AST and CK of Labrador retriever.
In order to study the effect of transport on dogs, Labrador retrievers were chosen and blood was collected before transport andafter 2-hour transport. Then serum cortisol, adrenocorticotropic hormone and biochemical indices were determined. The results showed: the concentration of cortisol and the activity of glutamic oxalacetic transaminase(AST) were significantly increased after transport(P<0.05). The activity of Creatine kinase(CK) was significantly increased(P<0.01). Adrenocorticotropic hormone(ACTH), alanine transaminase(ALT), alkaline phosphatase(ALP), lactate dehydrogenase(LDH), glucose(GLU) had no significant difference(P>0.05). In conclusion, 2-hour transport stress can induce the change of serum cortisol, AST and CK of Labrador retriever.
引文
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[15]Ochi T,Nishiura I,Tatsumi M,et al.Effects of transport stress on serum alkaline phosphatase activity in beagle dogs[J].Experimental Animals,2013,62(4):329-332.
[2]González L A,Schwartzkopf-Genswein K S,Bryan M,et al.Factors affecting body weight loss during commercial long haul transport of cattle in North America[J].Journal of Animal Science,2012,90(10):3630-3639.
[3]Sackett G P.Pregnancy outcome following jet transport stress in nonhuman primates[J].Journal of Medical Primatology,1981,10(2-3):149-154.
[4]Agnes F,Sartorelli P,Abdi B H,et al.Effect of transport loading or noise on blood biochemical variables in calves[J].American Journal of Veterinary Research,1990,51(10):1679-1681.
[5]Srithunyarat T,Hagman R,H?glund O V,et al.Catestatin,vasostatin,cortisol,and visual analog scale scoring for stress assessment in healthy dogs[J].Research in Veterinary Science,2017,22(117):74-80.
[6]Kuhn G,Lichtwald K,Hardegg K,et al.The effect of transportation stress on circulating corticosteroids,enzyme activities and hematological values in laboratory dogs[J].Journal of Experimental Animal Science,1991,34(3):99-104.
[7]Bergeron R,Scott S L,Emond J P,et al.Physiology and behavior of dogs during air transport[J].The Canadian Journal of Veterinary Research,2002,66(3):211-216.
[8]Britton C V,Hemandez A,Roberts R.Plasma creatine kinase isoenzyme determinations in infants and children[J].Chest,1980,77(6):758-760.
[9]Fabrega E,Manteca X,Font J,et al.Effects of halothane gene and pre-slaughter treatment on meat quality and welfare from two pig crosses[J].Meat Seience,2002,62(4):463-472.
[10]Li L A,Xia D,Bao E D,et al.Erhualian and Pietrain pigs exhibit distinct behavioral,endocrine and biochemical responses during transport[J].Livestock Seience,2007,113(2-3):169-177.
[11]盛颖,宗春华,陈媚疲,等.血清谷草转氨酶极度升高的病因学调查闭[J].中华消化杂志,1999,19(l):54.
[12]黄丽波,李双武,孙牧文,等.不同强度冷应激对白凤雏鸡血清生化指标的影响[J].天津农学院学报,2017,24(3):59-63.
[13]Miranda-de la Lama G C,Rodríguez-Palomares M,Cruz-Monterrosa R G,et al.Long-distance transport of hair lambs:effect of location in pot-belly trailers on thermo-physiology,welfare and meat quality[J].Tropical Animal Health and Production,2018,50(2):327-336.
[14]李玉保,孙培明,王志亮,等.运输应激猪组织病理性损伤、HSP27的组织分布及其相关性研究[J].南京农业大学学报,2005(3):83-87.
[15]Ochi T,Nishiura I,Tatsumi M,et al.Effects of transport stress on serum alkaline phosphatase activity in beagle dogs[J].Experimental Animals,2013,62(4):329-332.