光动力对瘢痕疙瘩的抑制效果研究
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  • 英文篇名:Inhibitory effect of photodynamic therapy on keloid
  • 作者:游传华 ; 王一贺 ; 陆雪飞
  • 英文作者:YOU Chuan-hua;WANG Yi-he;LU Xue-fei;Department of plastic and aesthetic surgery, the first affiliated hospital of hainan medical college;Department of anesthesiology, the first affiliated hospital of hainan medical college;
  • 关键词:光动力 ; 氨基酮戊酸 ; 瘢痕疙瘩 ; 细胞毒 ; 上皮-间质转化
  • 英文关键词:photodynamic;;aminoketovaleric acid;;keloid;;cytotoxicity;;epithelial-mesenchymal transition
  • 中文刊名:YLMR
  • 英文刊名:China Medical Cosmetology
  • 机构:海南医学院第一附属医院整形美容外科;海南医学院第一附属医院麻醉科;
  • 出版日期:2019-05-15
  • 出版单位:中国医疗美容
  • 年:2019
  • 期:v.9;No.69
  • 基金:海南省卫生计生行业科研项目(编号16A200116)
  • 语种:中文;
  • 页:YLMR201905015
  • 页数:4
  • CN:05
  • ISSN:11-6007/R
  • 分类号:61-64
摘要
目的通过观察不同浓度光敏剂氨基酮戊酸联合激光作用下瘢痕疙瘩各项指标变化情况,探讨光动力对瘢痕疙瘩的抑制作用。方法将15只健康裸鼠随机均分为实验组1、实验组2和对照组3组,背部分别植入瘢痕疙瘩组织形成稳定病理瘢痕,在第4、8、12周内分别外敷浓度10%氨基酮戊酸溶液、20%氨基酮戊酸溶液、生理盐水半小时,635nm波长连续波激光照射,第6、10、14周切取瘢痕组织,检测瘢痕相关指标。结果实验组瘢痕指数、成纤维细胞数量、TGF-β1蛋白数量、α-SMA等指标均低于对照组,实验组2以上指标低于实验组1,各组间差异有统计学意义。结论 20%氨基酮戊酸在光动力作用下产生细胞毒作用,诱导成纤维细胞凋亡,调控减少瘢痕上皮-间质转化,对瘢痕疙瘩产生抑制作用。
        Objective To investigate the inhibitory effect of photodynamic on keloid by observing the changes of various indicators of keloid under the action of different concentration of photosensitizer aminoketovaleric acid combined with laser. Methods Fifteen healthy nude mice were randomly divided into experimental group 1, experimental group 2 and control group. Keloid tissue was implanted into the back to form stable pathological scars. 10% aminoketovaleric acid solution, 20% aminoketovaleric acid solution,saline solution were applied to the back of the nude mice within 4, 8 and 12 weeks, respectively, for half an hour, 635 nm CW laser irradiation, and scar tissue was cut at 6, 10 and 14 weeks for detection. Scar related indicators. Results Scar index, number of fibroblasts, number of TGF-beta 1 protein and alpha-SMA in the experimental group were lower than those in the control group, and more than 2 indexes in the experimental group were lower than those in the experimental group 1. There was significant difference among the groups. Conclusion 20% aminoketovaleric acid can produce cytotoxic effect, induce apoptosis of fibroblasts, regulate and reduce epithelial-mesenchymal transition of scar, and inhibit keloid.
引文
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