视安利胶囊的制备及其含量测定
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摘要
目的:制备具有舒缓眼睛疲劳的视安利胶囊并对其主药进行含量测定。方法:以莲子壳提取物原花青素、叶黄素为主药制备视安利胶囊,采用HPLC法测定视安利胶囊中原花青素及叶黄素的含量。结果:所制备的样品为硬胶囊,除去胶囊壳后内容物为黄棕色至红棕色的颗粒,味微苦、涩。原花青素、叶黄素检测质量浓度的线性范围分别为15.03~302.42μg/m L(Y=1.54X-17.02,r=0.9993)、5.23~49.76μg/m L(Y=80.45X+1.83,r=0.9997)。平均回收率分别为100.5%(RSD=1.15%,n=6)、100.8%(RSD=0.73%,n=6)。结论:该制剂的处方和制备工艺合理可行,含量测定方法准确,可用于视安利胶囊的质量控制。
Objective: To prepare Shianli capsule which can relieve eye fatigue and the main medicine for content determination. Methods: The Shianli capsules were prepared by the extraction of Proanthocyanidins from lotus seed shell and lutein. The content of lutein and proanthocyanidins in optical amway capsules was determined by HPLC. Results: The preparation of samples for the hard capsule,remove the capsule shell contents after particles for yellow brown to reddish brown,slightly bitter,astringent. The linear range of the concentration of lutein was 15. 03 to 302. 42μg/m L(Y = 1. 54 X-17. 02,r = 0. 9993). The average recovery was 100. 5%(RSD = 1.15%,n = 6). The linear range of the concentration of proanthocyanidins was 15. 03 to 302. 42μg/m L(Y = 80. 45 X + 1. 83,r = 0.9997). The average recovery was 100. 8%(RSD = 0. 73%,n = 6). Conclution: The preparations of the prescription and preparation technology is reasonable,content determination method is accurate and can be used to regard the amway capsules for quality control.
Objective: To prepare Shianli capsule which can relieve eye fatigue and the main medicine for content determination. Methods: The Shianli capsules were prepared by the extraction of Proanthocyanidins from lotus seed shell and lutein. The content of lutein and proanthocyanidins in optical amway capsules was determined by HPLC. Results: The preparation of samples for the hard capsule,remove the capsule shell contents after particles for yellow brown to reddish brown,slightly bitter,astringent. The linear range of the concentration of lutein was 15. 03 to 302. 42μg/m L(Y = 1. 54 X-17. 02,r = 0. 9993). The average recovery was 100. 5%(RSD = 1.15%,n = 6). The linear range of the concentration of proanthocyanidins was 15. 03 to 302. 42μg/m L(Y = 80. 45 X + 1. 83,r = 0.9997). The average recovery was 100. 8%(RSD = 0. 73%,n = 6). Conclution: The preparations of the prescription and preparation technology is reasonable,content determination method is accurate and can be used to regard the amway capsules for quality control.
引文
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[2]M A Riva,C Arpa and M Gioco.Dante and asthenopia:a modern visual problem described during the Middle Ages[J].Eye,2014,28(4):498.
[3]Samuel O.Wajuihian.Frequency of asthenopia and its association with refractive errors[J].African Vision and Eye Health,2015,74(1):1 001.
[4]张玉,杨先,姜丽萍,等.BMP-2在C57BL/6小鼠形觉剥夺性近视眼巩膜中表达的变化[J].国际眼科杂志,2016,16(3):423.
[5]林巧雅,周罕英,李学喜.近视与眼相对调节力的相关性研究[J].国际眼科杂志,2015,15(7):1 234.
[6]张慧文,张玉,马超美.原花青素的研究进展[J].食品科学,2015(5):296.
[7]曹广美,赵桂森,贺艳丽.叶黄素的视力保护作用及提取工艺[J].食品与药品,2012,14(5):199.
[8]张良,王燕斐,刘宏生,等.天然植物高分子药用胶囊的研究与发展[J].高分子学报,2013(1):1-10.
[9]刘莉,刘强.小儿热清按摩凝胶的制备及质量控制[J].中华中医药杂志,2010,25(3):431-433.
[10]李小万,陈文,周俊,等.葡萄籽多酚泡腾颗粒制备工艺及含量测定[J].中成药,2016,38(4):937-940.
[11]刘奇,张玲,郎玉麟,等.郎氏扶正颗粒的制备及质量标准研究[J].中华中医药学刊,2014,32(1):196-199.