摘要
背景:目前3D生物打印技术可用于打印无细胞材料和含细胞材料,在解决器官、补片短缺的问题上开辟了新方法。目的:综述近年国内外有关3D挤压成型生物打印的研究,总结该打印方法的原理、所用水凝胶种类、水凝胶的生物理化性能等最新进展。方法:以"3D bioprinting/three-dimensional bioprinting;extrusion/extrusive;cell-laden/cells/cellular;hydrogel"为检索词,应用计算机搜索Pub Med数据库2006至2016年的相关文献。结果与结论:3D挤压成型生物打印技术生产原理简单,过程可控,产能较少,不仅可通过快速成型个性化地制造器官支架,而且可使细胞可控地分布于支架中,更加精确地构建符合生理空间的含细胞组织。然而,细胞在水凝胶内的生物学行为、水凝胶与细胞之间的相互作用、打印精度微纳米级别的提升目前仍在探索和研究阶段。随着这些问题的深入探讨和逐步解决,3D生物打印有望在未来成为组织工程学中的新的构建方法。
BACKGROUND: 3D bioprinting technology can be used to print non-cell and cell-laden materials, which provides a new pathway to solve the lack of transplanted organs or bio-patches.OBJECTIVE: To conclude the printing mechanisms, kinds, biological and mechanical characteristics of hydrogels based on the development and advance in 3D extrusive bioprinting hydrogel. METHODS: A computer-based search of Pub Med database was performed to retrieve relevant articles published between 2006 and 2016, with the keywords of "3D bioprinting/three-dimensional bioprinting; extrusion/extrusive; cell-laden/cells/cellular; hydrogel". RESULTS AND CONCLUSION: 3D bioprinting is characterized as simple principle, controlled process, and less energy production, which can manufacture personalized organs or scaffolds by rapid prototyping technology, and precisely construct tissues with controllably distributed cells to mimics the physiological circumstance. However, the following aspects are still at an initial stage, including the biological behaviors of cells in hydrogel, the interactions between cells and hydrogels and the elevation of micro-nano precision of printing. With solutions to these problems, 3D bioprinting may become another novel construction method in the tissue engineering.
引文
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