人Autotaxin基因3个蛋白亚型的生物信息学比较分析
|
摘要
同一基因编码产生的不同蛋白质亚型通常发挥着不同的生物学功能,利用公共数据库及各类预测软件,对Autotaxin基因编码产生的3个蛋白亚型ATX-α、ATX-β、ATX-γ进行生物信息学分析,从蛋白质的序列、二级结构、理化性质、亲疏水性、结构域、跨膜区域等方面进行比较,结果发现三者在各个方面有很大的相似性,除了跨膜区域外,三者各个方面均存在差异,这些差异可能导致3个蛋白亚型在功能上的不同。
引文
[1]Stracke M L,Krutzsch H C,Unsworth E J,et al.Identification,purification,and partial sequence analysis of autotaxin,a novel motility-stimulating protein.The Journal of biological chemistry,1992,267(4):2524.
[2]Bollen M,Gijsbers R,Ceulemans H,et al.Nucleotide pyrophosphatases/phosphodiesterases on the move.Critical reviews in biochemistry and molecular biology,2000,35(6):393.
[3]Kohn E C,Hollister G H,DiPersio J D,et al.Granulocytemacrophage colony-stimulating factor induces human melanomacell migration.International Journal of Cancer,1993,53(6):968.
[4]Kehlen A,Englert N,Seifert A,et al.Expression,regulation and function of autotaxin in thyroid carcinomas.International Journal of Cancer,2004,109(6):833.
[5]Yang S Y,Lee J,Park C G,et al.Expression of autotaxin(NPP-2)is closely linked to invasiveness of breast cancer cells.Clinical experimental metastasis,2002,19(7):603.
[6]Yang Y,Mou L J,Liu N,et al.Autotaxin expression in nonsmall-cell lung cancer.American journal of respiratory cell and molecular biology,1999,21(2):216.
[7]Zhang G,Zhao Z,Xu S,et al.Expression of autotoxin mRNAin human hepatocellular carcinoma.Chinese medical journal,1999,112(4):330.
[8]Nakai Y,Ikeda H,Nakamura K,et al.Specific increase in serum autotaxin activity in patients with pancreatic cancer.Clinical biochemistry,2011,44(8-9):576.
[9]Yasunori Tokuhara,Makoto Kurano,Satoshi Shimamoto,et al.A New Enzyme Immunoassay for the quantitative determination of classical autotaxins(ATXα,ATXβ,and ATXγ)and novel autotaxins(ATXδand ATXε).Plos One,2015,10(6):1.
[10]李宗伟.自分泌运动因子-溶血磷脂酸轴的生物学功能与调控.生理学报,2011,63(6):601.
[11]X Robert,P Gouet.Deciphering key features in protein structures with the new ENDscript server.Nucleic Acids Research,2014,42(Web Server issue):W320.
[12]Letunic I,Doerks T,Bork P.SMART:recent updates,new developments and status in 2015.Nucleic Acids Research.2015,43(Database issue),D257.
[13]Server O T E,Gasteiger E,Hoogl C,et al.Protein analysis tools on the ExPASy Server.Methods in Molecular Biology,1999,112(112):531.
[14]A.Krogh,B.Larsson,G.von Heijne,et al.Predicting transmembrane protein topology with a hidden Mar kov model:Application to complete genomes.Journal of Molecular Biology,2001,305(3):567.
[15]Yu Xia,Marc Vidal.Widespread expansion of protein interaction capabilities by alternative splicing.Cell,2016,164(4),805.
[2]Bollen M,Gijsbers R,Ceulemans H,et al.Nucleotide pyrophosphatases/phosphodiesterases on the move.Critical reviews in biochemistry and molecular biology,2000,35(6):393.
[3]Kohn E C,Hollister G H,DiPersio J D,et al.Granulocytemacrophage colony-stimulating factor induces human melanomacell migration.International Journal of Cancer,1993,53(6):968.
[4]Kehlen A,Englert N,Seifert A,et al.Expression,regulation and function of autotaxin in thyroid carcinomas.International Journal of Cancer,2004,109(6):833.
[5]Yang S Y,Lee J,Park C G,et al.Expression of autotaxin(NPP-2)is closely linked to invasiveness of breast cancer cells.Clinical experimental metastasis,2002,19(7):603.
[6]Yang Y,Mou L J,Liu N,et al.Autotaxin expression in nonsmall-cell lung cancer.American journal of respiratory cell and molecular biology,1999,21(2):216.
[7]Zhang G,Zhao Z,Xu S,et al.Expression of autotoxin mRNAin human hepatocellular carcinoma.Chinese medical journal,1999,112(4):330.
[8]Nakai Y,Ikeda H,Nakamura K,et al.Specific increase in serum autotaxin activity in patients with pancreatic cancer.Clinical biochemistry,2011,44(8-9):576.
[9]Yasunori Tokuhara,Makoto Kurano,Satoshi Shimamoto,et al.A New Enzyme Immunoassay for the quantitative determination of classical autotaxins(ATXα,ATXβ,and ATXγ)and novel autotaxins(ATXδand ATXε).Plos One,2015,10(6):1.
[10]李宗伟.自分泌运动因子-溶血磷脂酸轴的生物学功能与调控.生理学报,2011,63(6):601.
[11]X Robert,P Gouet.Deciphering key features in protein structures with the new ENDscript server.Nucleic Acids Research,2014,42(Web Server issue):W320.
[12]Letunic I,Doerks T,Bork P.SMART:recent updates,new developments and status in 2015.Nucleic Acids Research.2015,43(Database issue),D257.
[13]Server O T E,Gasteiger E,Hoogl C,et al.Protein analysis tools on the ExPASy Server.Methods in Molecular Biology,1999,112(112):531.
[14]A.Krogh,B.Larsson,G.von Heijne,et al.Predicting transmembrane protein topology with a hidden Mar kov model:Application to complete genomes.Journal of Molecular Biology,2001,305(3):567.
[15]Yu Xia,Marc Vidal.Widespread expansion of protein interaction capabilities by alternative splicing.Cell,2016,164(4),805.