生姜精油的超高效合相色谱特征图谱研究
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摘要
应用超高效合相色谱(UPC~2)技术研究了生姜精油的特征图谱。生姜精油经正庚烷稀释,以超临界CO_2为流动相,乙腈为助溶剂,采用超高效合相色谱梯度洗脱分离,流速为0.8 mL/min,二极管阵列(PDA)检测器测定,建立了特征峰PDA光谱谱图库及安徽产生姜精油的标准对照特征图谱。通过特征峰PDA光谱图检索识别及色谱图相似度评价,10份安徽产生姜精油的样品谱图与标准对照特征图谱的相似度均大于0.9。各共有指纹峰相对保留时间和相对峰面积的相对标准偏差(RSD)均小于3.0%,方法精密度、重现性、稳定性良好,符合特征图谱评价要求。通过超高效合相色谱特征图谱采集,结合聚类分析、主成分分析,对20批不同来源的生姜精油样品成功进行了区分。该研究为生姜精油的质量控制及产地溯源提供了一种简便、快速的新方法。
Specific chromatogram of ginger essential oil was studied by ultra performance convergence chromatographic(UPC~2) method.The ginger essential oil was diluted with n-heptane,and separated on UPC~2 system with photo-diode array(PDA) detector,using supercritical CO_2 as mobile phase and acetonitrile as cosolvent at a flow rate of 0.8 mL/min by gradient elution.A spectral library for characteristic peaks and standard specific chromatogram of ginger essential oil from Anhui was established.By comparison with the characteristic peaks from PDA library and similarity evaluation,the resemblance of specific chromatogram was found higher than 0.9 among the ginger essential oil from Anhui and the standard control.The relative standard deviations(RSD) for relative retention time and relative peak area of common peaks were less than 3.0%.This method has good precision,reproducibility and stability,and could meet the requirements for the evaluation of specific chromatogram.Coupled with cluster analysis and principal component analysis,the UPC~2 specific chromatogram method was successfully used to distinguish 20 samples from different habitats.This study provided a novel,convenient and rapid method for the quality control and origin traceability of ginger essential oil.
Specific chromatogram of ginger essential oil was studied by ultra performance convergence chromatographic(UPC~2) method.The ginger essential oil was diluted with n-heptane,and separated on UPC~2 system with photo-diode array(PDA) detector,using supercritical CO_2 as mobile phase and acetonitrile as cosolvent at a flow rate of 0.8 mL/min by gradient elution.A spectral library for characteristic peaks and standard specific chromatogram of ginger essential oil from Anhui was established.By comparison with the characteristic peaks from PDA library and similarity evaluation,the resemblance of specific chromatogram was found higher than 0.9 among the ginger essential oil from Anhui and the standard control.The relative standard deviations(RSD) for relative retention time and relative peak area of common peaks were less than 3.0%.This method has good precision,reproducibility and stability,and could meet the requirements for the evaluation of specific chromatogram.Coupled with cluster analysis and principal component analysis,the UPC~2 specific chromatogram method was successfully used to distinguish 20 samples from different habitats.This study provided a novel,convenient and rapid method for the quality control and origin traceability of ginger essential oil.
引文
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