延胡索酸对妊娠后期山羊营养物质表观消化率、瘤胃挥发性脂肪酸含量、血清生化指标和繁殖性能的影响
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摘要
本试验旨在研究延胡索酸对妊娠后期山羊营养物质表观消化率、瘤胃挥发性脂肪酸含量、血清生化指标和繁殖性能的影响。选用20只体重(36.5±2.5) kg、2～3岁、处于妊娠期90 d的湘东黑山羊,随机分成2组,每组10只。2组分别饲喂基础饲粮(对照组)和基础饲粮+2%的延胡索酸(延胡索酸组)。预试期为妊娠期90～94 d,正试期为妊娠期95 d至产羔。结果表明:与对照组相比,延胡索酸组妊娠后期山羊营养物质采食和表观消化率无显著变化(P>0.05);采食后2.5 h瘤胃中乙酸、丙酸、总挥发性脂肪酸含量和丙酸比例显著升高(P<0.05),乙丙比显著下降(P<0.05);血清丙二醛、雌二醇和雌三醇含量显著降低(P<0.05),血清催乳素、孕酮含量和过氧化氢酶活性显著升高(P<0.05);母羊妊娠后期增重有升高趋势(0.05≤P<0.10),羔羊初生总重显著增加(P<0.05)。由此可见,饲粮中添加2%的延胡索酸,可以提高妊娠后期山羊瘤胃中总挥发性脂肪酸含量,发酵模式向丙酸型转变,并有助于提高机体抗氧化能力、母羊妊娠后期增重和羔羊初生总重。
The aim of this study was to investigate the effects of fumarate on nutrient apparent digestibility,rumen volatile fatty acid content,serum biochemical parameters and reproductive performance of goats in later pregnancy.Twenty Xiangdong black goats in pregnancy 90 d,weighed(36.5±2.5) kg and aged between 2 and 3 years,were randomly divided into 2 groups,each group contained 10 goats.Goats in 2 groups were fed the basal diet(control group) and basal diet supplemented with 2% fumarate(fumarate group),respectively.The pre-experimental period lasted from 90 to 95 d in pregnancy,and the experimental period lasted from 95 d in pregnancy to birth of young goats.The results showed that compared with the control group,the nutrient feed intake and apparent digestibility of goats in later pregnancy of fumarate group were no significant change(P>0.05);on 2.5 h after feeding,the acetate,propionate,total volatile fatty acid contents and propionate percentage in rumen were significantly increased(P<0.05),the acetate to propionate ratio was significantly decreased(P<0.05);the contents of malondialdehyde,estradiol and estriol in serum were significantly decreased(P<0.05),the prolactin and progesterone contents and catalase activity in serum were significantly increased(P<0.05);the body weight gain of ewes during later pregnancy tended to increase(0.05≤P<0.10),the total birth weight of lambs was significantly increased(P<0.05).In conclusion,diet supplemented with2% fumarate can increase total volatile fatty acid content of goats in later pregnancy,and shift fermentation pathways from acetate to propionate,and improve the body antioxidant capacity,body weight gain of ewes during later pregnancy gain and total birth weight of lambs.
The aim of this study was to investigate the effects of fumarate on nutrient apparent digestibility,rumen volatile fatty acid content,serum biochemical parameters and reproductive performance of goats in later pregnancy.Twenty Xiangdong black goats in pregnancy 90 d,weighed(36.5±2.5) kg and aged between 2 and 3 years,were randomly divided into 2 groups,each group contained 10 goats.Goats in 2 groups were fed the basal diet(control group) and basal diet supplemented with 2% fumarate(fumarate group),respectively.The pre-experimental period lasted from 90 to 95 d in pregnancy,and the experimental period lasted from 95 d in pregnancy to birth of young goats.The results showed that compared with the control group,the nutrient feed intake and apparent digestibility of goats in later pregnancy of fumarate group were no significant change(P>0.05);on 2.5 h after feeding,the acetate,propionate,total volatile fatty acid contents and propionate percentage in rumen were significantly increased(P<0.05),the acetate to propionate ratio was significantly decreased(P<0.05);the contents of malondialdehyde,estradiol and estriol in serum were significantly decreased(P<0.05),the prolactin and progesterone contents and catalase activity in serum were significantly increased(P<0.05);the body weight gain of ewes during later pregnancy tended to increase(0.05≤P<0.10),the total birth weight of lambs was significantly increased(P<0.05).In conclusion,diet supplemented with2% fumarate can increase total volatile fatty acid content of goats in later pregnancy,and shift fermentation pathways from acetate to propionate,and improve the body antioxidant capacity,body weight gain of ewes during later pregnancy gain and total birth weight of lambs.
引文
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[6]NEWBOLD C J,LPEZ S,NELSON N,et al.Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro[J].British Journal of Nutrition,2007,94(1):27-35.
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[18]WANG M,WANG R,JANSSEN P H,e t al.Sampling procedure for the measurement of dissolved hydrogen and volatile fatty acids in the rumen of dairy cows[J].Journal of Animal Science,2016,94(3):1159-1169.
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[27]ROA ENGEL C A,STRAATHOF A J J,ZIJLMANST W,et al.Fumaric acid production by fermentation[J].Applied Microbiology and Biotechnology,2008,78(3):379-389.
[28]秦枫,潘孝青,邵乐,等.延胡索酸对夏季幼兔生长性能、空肠发育及血清抗氧化和免疫指标的影响[J].动物营养学报,2018,30(3):1103-1109.
[29]周岭,丁雪梅,罗玉衡,等.复合酸化剂和微生态制剂对蛋鸡生产性能、血液生化指标、抗氧化指标及沙门氏菌感染的影响[J].动物营养学报,2016,28(8):2571-2580.
[30]王玉琴,赵有璋,李发弟,等.三种生殖激素和细胞因子在绵羊妊娠期的变化[J].西北农业学报,2007,16(3):11-14.
[31]LI S A,LI J J.Estrogen-induced progesterone receptor in the syrian hamster kidney.I.Modulation by antiestrogens and aindrogens[J].Endocrinology,1978,103(6):2119-2128.
[32]LI J J,LI S A.Estrogen-induced progesterone receptor in the syrian hamster kidney.IL modulation by synthetic progestins[J].Endocrinology,1981,108(5):1751-1756.
[33]SCHWARTZ S M,BLAUSTEIN J D,WADE G N.Inhibition of estrous behavior by progesterone in rats:role of neural estrogen and progestin receptors[J].Endocrinology,1979,105(5):1078-1082.
[34]CHRISTENSON L K,SHORT R E,FORD S P.Effects of ingestion of ponderosa pine needles by latepregnant cows on uterine blood flow and steroid secretion[J].Journal of Animal Science,1992,70(2):531-537.
[35]岳康宁,李秋凤,曹玉凤,等.不同能量水平日粮对淘汰荷斯坦育成母牛生长性能和屠宰性能的影响[J].中国畜牧兽医,2018,45(2):392-399.
[2]CHEN X L,DONG X X,WANG Y C,et al.Mitochondrial engineering of the TCA cycle for fumarate production[J].Metabolic Engineering,2015,31:62-73.
[3]UNGERFELD E M,KOHN R A,WALLACE R J,et al.A meta-analysis of fumarate effects on methane production in ruminal batch cultures[J].Journal of Animal Science,2007,85(10):2556-2563.
[4]SMITH M A,MENDZ G L,JORGENSEN M A,et al.Fumarate metabolism and the microaerophily of Campylobacter species[J].The International Journal of Biochemistry&Cell Biology,1999,31(9):961-975.
[5]RIEDE S,BOGUHN J,BREVES G.Studies on potential effects of fumaric acid on rumen microbial fermentation,methane production and microbial community[J].Archives of Animal Nutrition,2013,67(5):368-380.
[6]NEWBOLD C J,LPEZ S,NELSON N,et al.Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro[J].British Journal of Nutrition,2007,94(1):27-35.
[7]ASANUMA N,IWAMOTO M,HINO T.Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro[J].Journal of Dairy Science,1999,82(4):780-787.
[8]SIROHI S K,PANDEY P,GOEL N.Response of fumaric Acid addition on methanogenesis,rumen fermentation,a nd dry matter degradability in diets containing wheat straw and sorghum or berseem as roughage source[J].ISRN Veterinary Science,2012,2012:496801.
[9]WOOD T A,WALLACE R J,ROWE A,et al.Encapsulated fumaric acid as a feed ingredient to decrease ruminal methane emissions[J].Animal Feed Science and Technology,2009,152(1/2):62-71.
[10]REMLING N,RIEDE S,LEBZIEN P,et al.Effects of fumaric acid on rumen fermentation,milk composition and metabolic parameters in lactating cows[J].Journal of Animal Physiology and Animal Nutrition,2014,98(5):968-981.
[11]王镜岩,朱圣庚,徐长法.生物化学[M].3版.北京:高等教育出版社,2002.
[12]邹思湘.动物生物化学[M].4版.北京:中国农业出版社,2005.
[13]汪玉松,邹思湘,张玉静.现代动物生物化学[M].北京:中国农业科技出版社,1999:120-152.
[14]郑集.普通生物化学[M].2版.北京:高等教育出版社,1985.
[15]HESS B W,LAKE S L,SCHOLLJEGERDES E J,et al.Nutritional controls of beef cow reproduction[J].Journal of Animal Science,2005,83(S1):E90-E106.
[16]NRC.Nutrient requirements of small ruminants:sheep,goats,cervids,and new world camelids[M].Washington D.C.:The National Academies Press,2007.
[17]张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.
[18]WANG M,WANG R,JANSSEN P H,e t al.Sampling procedure for the measurement of dissolved hydrogen and volatile fatty acids in the rumen of dairy cows[J].Journal of Animal Science,2016,94(3):1159-1169.
[19]WANG M,SUN X Z,JANSSEN P H,et al.Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures[J].Animal Feed Science and Technology,2014,194:1-11.
[20]赵小明.延胡索酸的营养作用与应用研究[J].武警工程学院学报,2004,20(4):8-10.
[21]于志勇.延胡索酸在饲料添加剂中的应用[J].江西饲料,2000(6):12-13.
[22]刘延贺,胡现生.延胡索酸与动物营养[J].郑州牧业工程高等专科学校学报,1994,14(3):30-39.
[23]刘南南,李宗军,靳纯嘏,等.日粮碳水化合物平衡指数和延胡索酸对山羊瘤胃发酵、微生物区系和甲烷产生的影响[C]//中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集.郑州:中国畜牧兽医学会动物营养学分会,2014:444.
[24]刘海涛,张爱忠,孙海洲,等.日粮中添加延胡索酸对奶牛体外瘤胃发酵的影响[J].家畜生态学报,2007,28(6):27-30.
[25]YU C W,CHEN Y S,CHENG Y H,et al.Effects of fumarate on ruminal ammonia accumulation and fiber digestion in vitro and nutrient utilization in dairy does[J].Journal of Dairy Science,2010,93(2):701-710.
[26]张耿,毛胜勇,朱伟云.高精料条件下延胡索酸对山羊瘤胃微生物体外发酵的影响[J].南京农业大学学报,2006,29(1):63-66.
[27]ROA ENGEL C A,STRAATHOF A J J,ZIJLMANST W,et al.Fumaric acid production by fermentation[J].Applied Microbiology and Biotechnology,2008,78(3):379-389.
[28]秦枫,潘孝青,邵乐,等.延胡索酸对夏季幼兔生长性能、空肠发育及血清抗氧化和免疫指标的影响[J].动物营养学报,2018,30(3):1103-1109.
[29]周岭,丁雪梅,罗玉衡,等.复合酸化剂和微生态制剂对蛋鸡生产性能、血液生化指标、抗氧化指标及沙门氏菌感染的影响[J].动物营养学报,2016,28(8):2571-2580.
[30]王玉琴,赵有璋,李发弟,等.三种生殖激素和细胞因子在绵羊妊娠期的变化[J].西北农业学报,2007,16(3):11-14.
[31]LI S A,LI J J.Estrogen-induced progesterone receptor in the syrian hamster kidney.I.Modulation by antiestrogens and aindrogens[J].Endocrinology,1978,103(6):2119-2128.
[32]LI J J,LI S A.Estrogen-induced progesterone receptor in the syrian hamster kidney.IL modulation by synthetic progestins[J].Endocrinology,1981,108(5):1751-1756.
[33]SCHWARTZ S M,BLAUSTEIN J D,WADE G N.Inhibition of estrous behavior by progesterone in rats:role of neural estrogen and progestin receptors[J].Endocrinology,1979,105(5):1078-1082.
[34]CHRISTENSON L K,SHORT R E,FORD S P.Effects of ingestion of ponderosa pine needles by latepregnant cows on uterine blood flow and steroid secretion[J].Journal of Animal Science,1992,70(2):531-537.
[35]岳康宁,李秋凤,曹玉凤,等.不同能量水平日粮对淘汰荷斯坦育成母牛生长性能和屠宰性能的影响[J].中国畜牧兽医,2018,45(2):392-399.