纳米氧化钛对小鼠卵巢功能的影响
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摘要
目的了解暴露纳米氧化钛对小鼠卵巢功能损伤情况。方法选择ICR雌性小鼠进行动物试验,研究纳米TiO_2颗粒暴露后小鼠血液抗缪勒氏管激素(AMH)的水平;并且检测卵巢组织AMH浓度。结果纳米TiO_2颗粒暴露后小鼠血液雌二醇、孕酮、AMH水平下降,卵巢中活性氧(O_2~(·-)、H_2O_2)大量产生,卵巢AMH浓度下降,并且在暴露90 d后改变显著。结论纳米氧化钛暴露导致小鼠卵巢储备功能下降,从而影响小鼠的生殖功能。
Objective Understanding the exposure of nano-titanium oxide leads to changes in AMH levels.Methods ICR female mice were used as experimental subjects to study the blood AMH level of mice exposed to nano-TiO_2 particles; and the AMH concentration of ovarian tissue was detected.Results After exposure to nano-TiO_2 particles, the levels of estradiol, progesterone and AMH in the blood decreased, and ROS(O_2~(·-),H_2O_2)in the ovary were abundantly produced. The concentration of ovarian AMH decreased, and the change was significant after 90 days of exposure.Conclusion Exposure to nano-titanium causes decrease in mouse ovarian reserve, which affects the reproductive function of mice.
Objective Understanding the exposure of nano-titanium oxide leads to changes in AMH levels.Methods ICR female mice were used as experimental subjects to study the blood AMH level of mice exposed to nano-TiO_2 particles; and the AMH concentration of ovarian tissue was detected.Results After exposure to nano-TiO_2 particles, the levels of estradiol, progesterone and AMH in the blood decreased, and ROS(O_2~(·-),H_2O_2)in the ovary were abundantly produced. The concentration of ovarian AMH decreased, and the change was significant after 90 days of exposure.Conclusion Exposure to nano-titanium causes decrease in mouse ovarian reserve, which affects the reproductive function of mice.
引文
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[10] Tremellen KP,Kolo M,Gilmore A,et al.Anti-mullerian hormone as a marker of ovarian reserve[J].Aust N Z J obstet Gynaecol,2005 ,45(1):20-24.
[11] Tsepelidis S,Devreker F,Demeestere I,et al.Stuble serum levels of anti-Mullerrian hormone during the menstrual cycle :a prospective study in normo-ovulatory women[J].Hum Reprod,2007,22(7):1837-1840.
[2] Cho M, Chung H, Choi W, et al. Linear correlation between inactivation of E. coli and OH radical concentration in TiO2 photocatalytic disinfection[J].Water Res, 2004, 38(4):1069-1077.
[3] Choi H, Stathatos E, Dionysiou DD. Solgel preparation of mesoporous photocatalytic TiO2 films and TiO2/Al2O3 composite membranes for environmental applications[J]. Appl Catal B-Environ, 2006, 63: 60-67.
[4] Esterkin CR, Negro AC, Alfano OM,et al. Air pollution remediation in a fixed bed photocatalytic reactor coated with TiO2[J].AIChE J,2005,51(8):2298-2310.
[5] 应贤平,仲伟鉴.纳米二氧化钛颗粒毒理学进展[J].毒理学杂志,2006,5:334-336.
[6] Richards JS, Rolfes AI. Hormonal regulation of cyclic AMP binding to specific receptor proteins in rat ovarian follicles[J]. J Biol Chem, 1980,(225):5481-5489.
[7] Hockenbery DM, Oltvai ZN, Yin XM, et al. Bcl-2 functions in an antioxidant pathway to prevent apoptosis[J].Cell,1993,(75):241-251.
[8] Nel A, Xia T, Madler L, et al. Toxic potential of materials at the nanolevel[J].Sci,2006,(311):622-627.
[9] Ueda S, Masutani H, Nakamura H, et al. Redox control of cell death, Antioxid[J].Redox Signal,2002,(4):405-414.
[10] Tremellen KP,Kolo M,Gilmore A,et al.Anti-mullerian hormone as a marker of ovarian reserve[J].Aust N Z J obstet Gynaecol,2005 ,45(1):20-24.
[11] Tsepelidis S,Devreker F,Demeestere I,et al.Stuble serum levels of anti-Mullerrian hormone during the menstrual cycle :a prospective study in normo-ovulatory women[J].Hum Reprod,2007,22(7):1837-1840.