桂桑优12种子油提取的响应面工艺优化及其抗氧化分析
|
摘要
【目的】优化桂桑优12种子油提取工艺条件,并分析其抗氧化活性,为广西主推桑树品种桂桑优12种子油提取和高值化利用提供技术支持。【方法】以桂桑优12种子为试验材料,种子油提取率为评价指标,从5个溶剂组合中筛选出提取桂桑优12种子油的最佳溶剂;并在单因素试验的基础上,将3个显著因素的最佳条件设为响应中心值,利用响应面法对种子油提取工艺进行优化,依据回归分析确定其最适提取条件;同时测定种子油对DPPH和羟基自由基的清除率。【结果】体积百分比为40%正己烷+60%石油醚的组合是提取桂桑优12种子油的适宜溶剂;各因素对桂桑优12种子油提取率的影响排序为提取温度>提取时间>提取溶剂体积;桂桑优12种子油的最佳提取工艺条件为:提取温度83℃、提取溶剂体积50 m L、提取时间4.0 h,在此条件下,得到桂桑优12种子油提取率为35.04%,与预测值(35.07%)接近;桂桑优12种子油对DPPH和羟基自由基均有较强的清除能力,其半数有效质量浓度(IC50)分别为2.111和0.196 mg/mL。【结论】响应面试验模型能较好优化桂桑优12种子油的提取工艺,优化后的工艺具有操作便捷、高效可行的优势,提取的种子油具有较强抗氧化能力。
【Objective】The extraction conditions of the seed oil of Guisangyou 12 mulberry were optimized,and the antioxidant activity was analyzed,in order to provide technical support for the extraction and high value utilization of mulberry seed oil from Guisangyou 12,which was the main mulberry variety in Guangxi.【Method】Guisangyou 12 mulberry seeds were used as raw material,and the extraction rate of mulberry seed oil as evaluation index. Five different solvent combinations were screened out as suitable extraction solvent for extracting Guisangyou 12 mulberry seed oil. On the basis of single factor experiment,three main factors were selected as the response center value to optimize the extraction process of Guisangyou 12 mulberry seed oil by response surface methodology,and the optimum conditions were determined by regression analysis. The DPPH and hydroxyl free radicals scavenge ability of seed were also determined.【Result】The volumn percentage of 40% hexane and 60% petroleum ether was the suitable solvent for the extraction of Guisangyou 12 mulberry seed oil. The effects of various factors on the extraction rate of Guisangyou 12 mulberry seed oil were as follows:extraction temperature>extraction time>extraction solvent volume. The optimal extraction conditions for extraction of Guisangyou 12 mulberry seed oil were as follows:extraction temperature 83 ℃,extraction solvent volume 50 mL,extraction time 4 h. Under this condition,the extraction rate of seed oil of Guisangyou 12 was 35.04%. It was close to the predicted value(35.07%). Furthermore,Guisangyou 12 mulberry seed oil had strong ability to scavenge DPPH and hydroxyl free radicals,and the half effective mass concentration(IC50)were 2.111 and 0.196 mg/mL respectively.【Conclusion】The response surface experimental model can optimize extraction process of Guisangyou 12 mulberry seed oil. The optimized process has the advantages of convenient operation,high efficiency and feasibility,and extracted Guisangyou12 mulberry seed oil has strong antioxidant capacity.
【Objective】The extraction conditions of the seed oil of Guisangyou 12 mulberry were optimized,and the antioxidant activity was analyzed,in order to provide technical support for the extraction and high value utilization of mulberry seed oil from Guisangyou 12,which was the main mulberry variety in Guangxi.【Method】Guisangyou 12 mulberry seeds were used as raw material,and the extraction rate of mulberry seed oil as evaluation index. Five different solvent combinations were screened out as suitable extraction solvent for extracting Guisangyou 12 mulberry seed oil. On the basis of single factor experiment,three main factors were selected as the response center value to optimize the extraction process of Guisangyou 12 mulberry seed oil by response surface methodology,and the optimum conditions were determined by regression analysis. The DPPH and hydroxyl free radicals scavenge ability of seed were also determined.【Result】The volumn percentage of 40% hexane and 60% petroleum ether was the suitable solvent for the extraction of Guisangyou 12 mulberry seed oil. The effects of various factors on the extraction rate of Guisangyou 12 mulberry seed oil were as follows:extraction temperature>extraction time>extraction solvent volume. The optimal extraction conditions for extraction of Guisangyou 12 mulberry seed oil were as follows:extraction temperature 83 ℃,extraction solvent volume 50 mL,extraction time 4 h. Under this condition,the extraction rate of seed oil of Guisangyou 12 was 35.04%. It was close to the predicted value(35.07%). Furthermore,Guisangyou 12 mulberry seed oil had strong ability to scavenge DPPH and hydroxyl free radicals,and the half effective mass concentration(IC50)were 2.111 and 0.196 mg/mL respectively.【Conclusion】The response surface experimental model can optimize extraction process of Guisangyou 12 mulberry seed oil. The optimized process has the advantages of convenient operation,high efficiency and feasibility,and extracted Guisangyou12 mulberry seed oil has strong antioxidant capacity.
引文
董迪迪,王鸿飞,周增群,张天龙,邵兴峰,许凤,杨娜,李和生.2014.杨梅籽油抗氧化活性及其调节血脂作用的研究[J].中国粮油学报,29(5):53-57.[Dong D D,Wang H F,Zhou Z Q,Zhang T L,Shao X F,Xu F,Yang N,Li H S.2014.Antioxidant activity and blood lipid regulation of Myrica rubra seed oil[J].Journal of the Chinese Cereals and Oils Association,29(5):53-57.]
胡翠珍,李胜,马绍英,苏利荣,曹宝臣,周文政,刘琦.2015.响应面试验优化葡萄籽油提取工艺及其抗氧化性[J].食品科学,36(20):56-61.[Hu C Z,Li S,Ma S Y,Su LR,Cao B C,Zhou W Z,Liu Q.2015.Optimization of extraction process for grape seed oil by response surface methodology and evaluation of its antioxidant properties[J].Food Science,36(20):56-61.]
胡青平.2010.超声波辅助提取桑椹籽油的工艺优化[J].中国粮油学报,25(10):69-73.[Hu Q P.2010.Optimization of ultrasonic-assisted extraction of oil from mulberry seeds[J].Journal of the Chinese Cereals and Oils Association,25(10):69-73.]
陆春霞,梁贵秋,吴婧婧,周晓玲,黄正勇,谭汪英,肖潇.2018.3种桑椹酒理化指标及营养成分比较分析[J].广西蚕业,55(1):34-36.[Lu C X,Liang G Q,Wu J J,Zhou X L,Huang Z Y,Tan W Y,Xiao X.2018.Comparative analysis of physical and chemical indicators and nutrient composition of three kinds of mulberry wine[J].Guangxi Sericulture,55(1):34-36.]
吕志强,李乔,巩丽丽,陈智,王超,田景振.2011.桑椹籽油提取工艺优选[J].中国实验方剂学杂志,17(17):45-47.[LüZ Q,Li Q,Gong L L,Chen Z,Wang C,Tian J Z.2011.Extraction process of the seed-oil from Morus alba[J].Chinese Journal of Experimental Traditional Medical Formulae,17(17):45-47.]
孟祥凯,孔凡刚,丁菲,巩丽丽.2015.DPPH法测定桑籽不同极性部位抗氧化性[J].食品与药品,17(4):255-258.[Meng X K,Kong F G,Ding F,Gong L L.2015.Antioxidant activity determination of different polar components extracted from mulberry seeds by DPPH method[J].Food and Drug,17(4):255-258.]
聂良文,祁广军,白景彰,潘启寿,罗坚,黄艺,曾森,唐燕梅,陈小青,宾荣佩.2013.亚热带超高产杂交桑品种配套技术及其在广西的推广应用[J].广西蚕业,50(4):31-35.[Nie L W,Qi G J,Bai J Z,Pan Q S,Luo J,Huang Y,Zeng S,Tang Y M,Chen X Q,Bin R P.2013.Matching technology for subtropical super-high-yield hybrid mulberry variety and its application in Guangxi[J].Guangxi Sericulture,50(4):31-35.]
王媛,王定颖,岳田利.2012.超声波提取葡萄籽油的工艺优化及其抗氧化性研究[J].食品科学,33(10):136-140.[Wang Y,Wang D Y,Yue T L.2012.Optimization of ultrasonic-assisted extraction and antioxidant activity of grape seed oil[J].Food Science,33(10):136-140.]
吴娱明,施英,肖更生,徐玉娟,邹宇晓,姚锡镇.2006.桑籽的营养成分研究[J].蚕业科学,32(1):135-137.[Wu Y M,Shi Y,Xiao G S,Xu Y J,Zou Y X,Yao X Z.2006.Studies on nutrient constituents in mulberry seeds[J].Science of Sericulture,32(1):135-137.]
徐建国,胡青平,王彦彦,罗季阳.2009.桑椹籽油的提取及其脂肪酸组成分析[J].中国粮油学报,24(5):88-90.[Xu J G,Hu Q P,Wang Y Y,Luo J Y.2009.Extraction technology and fatty acid composition of mulberry seed oil[J].Journal of the Chinese Cereals and Oils Association,24(5):88-90.]
徐周徐,屈达才.2014.“两广二号”和“桂桑优12”等广西主推蚕桑品种DNJ含量测定分析[J].广西农学报,29(4):23-29.[Xu Z X,Qu D C.2014.Determination and analysis of DNJ content in silkworm powders of the main varieties in Guangxi[J].Journal of Guangxi Agriculture,29(4):23-29.]
杨小兰.2011.桑椹籽油的降脂作用研究[J].中国粮油学报,16(4):8-9.[Yang X L.2011.Study on hypolipidemic effects of mulberry seed oil[J].Journal of the Chinese Cereals and Oils Association,16(4):8-9.]
杨小兰,周纪侃,马文丽,1998.桑椹籽与籽油的营养成份及理化特性的研究[J].中国粮油学报,13(4):43-45.[Yang X L,Zhou J K,Ma W L.1998.The composition and some characteristics of the seeds and the seed-oil of Morus alba L.[J].Journal of the Chinese Cereals and Oils Association,13(4):43-45.]
张志伟,杨中平,岳田利,高振红.2007.超临界CO2流体萃取桑椹籽油的研究[J].中国油脂,32(1):77-80.[Zhang ZW,Yang Z P,Yue T L,Gao Z H.2007.Supercritical CO2fluid extraction of mulberry seed oil[J].China Oils and Fats,32(1):77-80.]
Ali I B E H,Chaouachi M,Bahri R,Chaieb I,Boussaid M,Harzallah-Skhiri F.2015.Chemical composition and antioxidant,antibacterial,allelopathic and insecticidal activities of essential oil of Thymus algerienis Boiss.et Reut[J].Industrial Crops and Products,77(23):631-639.
胡翠珍,李胜,马绍英,苏利荣,曹宝臣,周文政,刘琦.2015.响应面试验优化葡萄籽油提取工艺及其抗氧化性[J].食品科学,36(20):56-61.[Hu C Z,Li S,Ma S Y,Su LR,Cao B C,Zhou W Z,Liu Q.2015.Optimization of extraction process for grape seed oil by response surface methodology and evaluation of its antioxidant properties[J].Food Science,36(20):56-61.]
胡青平.2010.超声波辅助提取桑椹籽油的工艺优化[J].中国粮油学报,25(10):69-73.[Hu Q P.2010.Optimization of ultrasonic-assisted extraction of oil from mulberry seeds[J].Journal of the Chinese Cereals and Oils Association,25(10):69-73.]
陆春霞,梁贵秋,吴婧婧,周晓玲,黄正勇,谭汪英,肖潇.2018.3种桑椹酒理化指标及营养成分比较分析[J].广西蚕业,55(1):34-36.[Lu C X,Liang G Q,Wu J J,Zhou X L,Huang Z Y,Tan W Y,Xiao X.2018.Comparative analysis of physical and chemical indicators and nutrient composition of three kinds of mulberry wine[J].Guangxi Sericulture,55(1):34-36.]
吕志强,李乔,巩丽丽,陈智,王超,田景振.2011.桑椹籽油提取工艺优选[J].中国实验方剂学杂志,17(17):45-47.[LüZ Q,Li Q,Gong L L,Chen Z,Wang C,Tian J Z.2011.Extraction process of the seed-oil from Morus alba[J].Chinese Journal of Experimental Traditional Medical Formulae,17(17):45-47.]
孟祥凯,孔凡刚,丁菲,巩丽丽.2015.DPPH法测定桑籽不同极性部位抗氧化性[J].食品与药品,17(4):255-258.[Meng X K,Kong F G,Ding F,Gong L L.2015.Antioxidant activity determination of different polar components extracted from mulberry seeds by DPPH method[J].Food and Drug,17(4):255-258.]
聂良文,祁广军,白景彰,潘启寿,罗坚,黄艺,曾森,唐燕梅,陈小青,宾荣佩.2013.亚热带超高产杂交桑品种配套技术及其在广西的推广应用[J].广西蚕业,50(4):31-35.[Nie L W,Qi G J,Bai J Z,Pan Q S,Luo J,Huang Y,Zeng S,Tang Y M,Chen X Q,Bin R P.2013.Matching technology for subtropical super-high-yield hybrid mulberry variety and its application in Guangxi[J].Guangxi Sericulture,50(4):31-35.]
王媛,王定颖,岳田利.2012.超声波提取葡萄籽油的工艺优化及其抗氧化性研究[J].食品科学,33(10):136-140.[Wang Y,Wang D Y,Yue T L.2012.Optimization of ultrasonic-assisted extraction and antioxidant activity of grape seed oil[J].Food Science,33(10):136-140.]
吴娱明,施英,肖更生,徐玉娟,邹宇晓,姚锡镇.2006.桑籽的营养成分研究[J].蚕业科学,32(1):135-137.[Wu Y M,Shi Y,Xiao G S,Xu Y J,Zou Y X,Yao X Z.2006.Studies on nutrient constituents in mulberry seeds[J].Science of Sericulture,32(1):135-137.]
徐建国,胡青平,王彦彦,罗季阳.2009.桑椹籽油的提取及其脂肪酸组成分析[J].中国粮油学报,24(5):88-90.[Xu J G,Hu Q P,Wang Y Y,Luo J Y.2009.Extraction technology and fatty acid composition of mulberry seed oil[J].Journal of the Chinese Cereals and Oils Association,24(5):88-90.]
徐周徐,屈达才.2014.“两广二号”和“桂桑优12”等广西主推蚕桑品种DNJ含量测定分析[J].广西农学报,29(4):23-29.[Xu Z X,Qu D C.2014.Determination and analysis of DNJ content in silkworm powders of the main varieties in Guangxi[J].Journal of Guangxi Agriculture,29(4):23-29.]
杨小兰.2011.桑椹籽油的降脂作用研究[J].中国粮油学报,16(4):8-9.[Yang X L.2011.Study on hypolipidemic effects of mulberry seed oil[J].Journal of the Chinese Cereals and Oils Association,16(4):8-9.]
杨小兰,周纪侃,马文丽,1998.桑椹籽与籽油的营养成份及理化特性的研究[J].中国粮油学报,13(4):43-45.[Yang X L,Zhou J K,Ma W L.1998.The composition and some characteristics of the seeds and the seed-oil of Morus alba L.[J].Journal of the Chinese Cereals and Oils Association,13(4):43-45.]
张志伟,杨中平,岳田利,高振红.2007.超临界CO2流体萃取桑椹籽油的研究[J].中国油脂,32(1):77-80.[Zhang ZW,Yang Z P,Yue T L,Gao Z H.2007.Supercritical CO2fluid extraction of mulberry seed oil[J].China Oils and Fats,32(1):77-80.]
Ali I B E H,Chaouachi M,Bahri R,Chaieb I,Boussaid M,Harzallah-Skhiri F.2015.Chemical composition and antioxidant,antibacterial,allelopathic and insecticidal activities of essential oil of Thymus algerienis Boiss.et Reut[J].Industrial Crops and Products,77(23):631-639.