不同处理方法对灵芝有效成分提取率的影响
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摘要
以灵芝为原料,以水为溶剂进行回流提取,比较粗碎、粉碎、切薄片及切厚片4种药材处理方式对灵芝主要有效成分提取率的影响。结果表明,不同药材处理方式对灵芝有效成分的提取率有明显影响,切薄片提取对灵芝多糖、三萜甾醇、灵芝酸的提取率及总出膏率明显高于其他3种处理方式。
Using Ganoderma lucidum as a raw material, the effects of four treatment methods, namely coarse crushing,smashing,slicing and cutting thick slices,the extraction ratio of major active ingredients of Ganoderma lucidum water extraction were compared. The results showed that the effect of different treatment methods on the extraction ratio of effective components of Ganoderma lucidum was significant. Slice Ganoderma lucidum thinly,the extraction ratios of polysaccharide,triterpenes and sterols,and ganoderic acid were significantly higher than the other three treatment.
Using Ganoderma lucidum as a raw material, the effects of four treatment methods, namely coarse crushing,smashing,slicing and cutting thick slices,the extraction ratio of major active ingredients of Ganoderma lucidum water extraction were compared. The results showed that the effect of different treatment methods on the extraction ratio of effective components of Ganoderma lucidum was significant. Slice Ganoderma lucidum thinly,the extraction ratios of polysaccharide,triterpenes and sterols,and ganoderic acid were significantly higher than the other three treatment.
引文
[1]国家药典委员会.中国药典(2015年版一部)[M].北京:中国医药科技出版社,2015:188-189.
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[6]Bojana Boh,Marin Berovic,Jingsong Zhang,et al.Ganoderma lucidum and its pharmaceutically active compounds[J].Biotechnology Annual,2007(13):265-268.
[7]刘晓艳,陈艺煊,吴林秀,等.菌草灵芝多糖提取工艺优化及抗氧化活动研究[J].农产品加工,2016(20):41-44,47.
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[10]江振友,林晨.灵芝多糖对小鼠体液免疫的影响[J].暨南大学学报(自然科学与医学版),2003,24(2):51-53.
[11]Jeurink P V,Noguera C L,Savelkoul H F,et al.Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells[J].Int Immunopharmacology,2008(8):1 124-1 133.
[12]曹立珍,林志彬.椴木栽培及袋栽灵芝多糖对体外培养小鼠脾淋巴细胞增殖活性的比较[J].药学学报,2003,38(2):92-97.
[13]刘晓艳,陈艺煊,吴林秀,等.响应面法优化灵芝总三萜酶辅助提取工艺及其抗氧化活性研究[J].食品科技,2017,42(8):225-230.
[14]白凤岐,牟建楼,陈志周,等.水提法提取灵芝多糖及其体外抗氧化研究[J].食品工业,2015,36(3):143-146.
[15]崔晓莹.灵芝中的三萜类化合物(灵芝酸)的提取条件优化及其结构分析[D].长春:东北师范大学,2007.
[2]徐阳阳.不同提取方法对灵芝活性成分提取率及抗氧化活性的影响[D].福州:福建农林大学,2014.
[3]刘高强,丁重阳.灵芝三萜类化合物抗癌作用机制的研究进展[J].菌物学报,2007,26(3):470-476.
[4]Kimura Y,Taniguchi M,Baba K.Antitumor and antimetastatic effects on liver of triterpenoid fractions of Ganoderma lucidum:Mechanism of action and isolation of an active substance[J].Anticancer Res,2002,22(6A):3 309-3 318.
[5]Mingyu W,Qiang L,Qingming C,et al.Effects of triterpenoids from Ganoderma lucidum(Leyss.Ex Fr).Karst on three different experimental liver injury models in mice[J].Acta Pharmaceutica Sinica,2000,35(5):326-329.
[6]Bojana Boh,Marin Berovic,Jingsong Zhang,et al.Ganoderma lucidum and its pharmaceutically active compounds[J].Biotechnology Annual,2007(13):265-268.
[7]刘晓艳,陈艺煊,吴林秀,等.菌草灵芝多糖提取工艺优化及抗氧化活动研究[J].农产品加工,2016(20):41-44,47.
[8]张群豪,於东晕.用血清药理学方法研究灵芝浸膏GLE的抑瘤机制[J].北京医科大学学报,2000(32):210-213.
[9]Ahmadi K,Riazipour M.Effect of Ganoderma lucidum on cytokine release by peritoneal macrophages[J].Iran.J.Immunol,2007(4):220-226.
[10]江振友,林晨.灵芝多糖对小鼠体液免疫的影响[J].暨南大学学报(自然科学与医学版),2003,24(2):51-53.
[11]Jeurink P V,Noguera C L,Savelkoul H F,et al.Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells[J].Int Immunopharmacology,2008(8):1 124-1 133.
[12]曹立珍,林志彬.椴木栽培及袋栽灵芝多糖对体外培养小鼠脾淋巴细胞增殖活性的比较[J].药学学报,2003,38(2):92-97.
[13]刘晓艳,陈艺煊,吴林秀,等.响应面法优化灵芝总三萜酶辅助提取工艺及其抗氧化活性研究[J].食品科技,2017,42(8):225-230.
[14]白凤岐,牟建楼,陈志周,等.水提法提取灵芝多糖及其体外抗氧化研究[J].食品工业,2015,36(3):143-146.
[15]崔晓莹.灵芝中的三萜类化合物(灵芝酸)的提取条件优化及其结构分析[D].长春:东北师范大学,2007.