黄芩对大黄蒽醌在提取精制过程中转化的影响
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摘要
目的探讨黄芩对大黄中5种蒽醌类成分在提取精制过程中相互转化的影响。方法 HPLC测定药材和提取物中5种成分的含量,比较药材和提取物中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的相对比例;再分别用5种蒽醌的对照品加入和不加黄芩药材提取液模拟提取精制过程,考察5种蒽醌成分之间的转化情况。结果大黄药材和提取物中5种成分的相对比例有明显变化,大黄酸在提取物中的比例增高;同时采用对照品模拟提取精制过程时各个成分并无转化,而加入黄芩药材提取液后,大黄酸经过提取精制处理后能部分转化为大黄素,其余4种成分之间无转化。结论黄芩和大黄药材配伍提取可以使大黄蒽醌类成分发生转化。
OBJECTIVE To investigate the reciprocal transformation of rhubarb five anthraquinones constituents in extracting and refining process. METHODS The method of HPLC was used to determine the content of five anthraquinones constituents in the ten batches of herbs and extracts respectively. And relative content of aloe-emodin, rhein, emodin, chrysophanol and physcion in rhubarb medicine material was compared with that in extracts of Rhubarb. Five anthraquinones reference substances were used to review the conversion between five anthraquinones constituentsin in the simulated extracting and refined process of rhubarb. RESULTS The ratio of five anthraquinones constituents in extracts had obviously changed compared with that in Rhubarb medicine material. The proportion of rhein increased in extract. But the transformation was not been found among the five reference substances. However, only rhein reference substance could partly be transformed into emodin in extracting and refined process with the addition of Scutellariae Radix extract close to Rhubarb in genetic relationship. CONCLUSION The results provide reference for the extracted and refined process of rhubarb anthraquinone study.
OBJECTIVE To investigate the reciprocal transformation of rhubarb five anthraquinones constituents in extracting and refining process. METHODS The method of HPLC was used to determine the content of five anthraquinones constituents in the ten batches of herbs and extracts respectively. And relative content of aloe-emodin, rhein, emodin, chrysophanol and physcion in rhubarb medicine material was compared with that in extracts of Rhubarb. Five anthraquinones reference substances were used to review the conversion between five anthraquinones constituentsin in the simulated extracting and refined process of rhubarb. RESULTS The ratio of five anthraquinones constituents in extracts had obviously changed compared with that in Rhubarb medicine material. The proportion of rhein increased in extract. But the transformation was not been found among the five reference substances. However, only rhein reference substance could partly be transformed into emodin in extracting and refined process with the addition of Scutellariae Radix extract close to Rhubarb in genetic relationship. CONCLUSION The results provide reference for the extracted and refined process of rhubarb anthraquinone study.
引文
[1]中国药典.一部[S].2010:22.
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[10]ZOU J L,XIE Z Y,JIANG X F,et al.Determination of anthraquinones in different rhubarb and coptis xiexin decoctions by HPLC[J].World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化),2008,10(04):61-65.
[11]WU B,LIU S,SUN Z L,et al.Analysis of plasmic and urinary constituents after oral administration of rheum palmatum,scutellaria baicalensis and their compatibility in rats[J].World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化),2010,12(4):652-656.
[12]LIU C Z,LIU X G,CHEN D W,et al.Studies on detecting method of Sanhuang Rapid-release Tablets[J].Chin J Pharm Anal(药物分析杂志),2005,25(8):929-931.
[13]MIAO G X,LI Z S,WU N N,et al.Processing quality of Scutellaria baicalensis[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,22(18):91-94.
[2]郑虎占,董泽宏,佘靖.中药现代研究与应用)[M].第1版.北京:学苑出版社,1997:364,390~395.
[3]LIU C Z,LIU X G,TONG J M,et al.Design and evaluation of San-huang dispersible tablet-an efficient delivery system for Traditional Chinese Medicine[J].Pharm Devel Technol,2009,14(5):506-515.
[4]刘翠哲.大黄有效成分提取工艺与三黄速释制剂及其制备方法:中国,CN1335293[P].2002-02-13.
[5]LIU P,TONG J M,LIU X G,et al.Bioadhesion and purgation effect of colon-specific microspheres of total anthraquinones in Rhei Radix et Rhizoma[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,22(04):165-168.
[6]HA F,LI R M,ZHANG L L,et al.Transformation relationship of four sennoside components in Rhei Radix et Rhizoma under the heating condition[J].Chin Tradit Pat Med(中成药),2012,34(11):2169-2173.
[7]GUO S,DUAN J A,WU D W,et al.Effect of drying method on transformation of bioactive constituents in Polygoni Multiflori Radix[J].Chin Tradit Herb Drugs(中草药),2014,45(4):498-503.
[8]QIN Q F.Determination of chlorogenic acid,forsythin,rhein,emodin and chrysophanol in Lianhuan Qingwen Capsules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(2):210-213.
[9]HE Y M,ZHANG Y,MENG X L,et al.Effective component changes in compatibility of Radix et Rhizoma Rhei with Radix Scutellariae in Xiexin Decoction[J].Chin Tradit Herb Drugs(中草药),2007,38(05):699-702.
[10]ZOU J L,XIE Z Y,JIANG X F,et al.Determination of anthraquinones in different rhubarb and coptis xiexin decoctions by HPLC[J].World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化),2008,10(04):61-65.
[11]WU B,LIU S,SUN Z L,et al.Analysis of plasmic and urinary constituents after oral administration of rheum palmatum,scutellaria baicalensis and their compatibility in rats[J].World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化),2010,12(4):652-656.
[12]LIU C Z,LIU X G,CHEN D W,et al.Studies on detecting method of Sanhuang Rapid-release Tablets[J].Chin J Pharm Anal(药物分析杂志),2005,25(8):929-931.
[13]MIAO G X,LI Z S,WU N N,et al.Processing quality of Scutellaria baicalensis[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,22(18):91-94.