不同基质和插穗粗度对醉香含笑扦插生根的影响
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摘要
[目的]筛选出醉香含笑的最佳扦插基质和插穗粗度,为产业化开发提供一定理论依据。[方法]以醉香含笑当年生枝条为试验材料,研究11种不同基质、3种规格插穗粗度对醉香含笑扦插生根的影响。[结果]基质和插穗粗度对醉香含笑扦插生根效果有显著影响。11种基质中,泥炭土为最佳基质;插穗在此基质上愈伤率为65.0%,生根率为28.3%,平均根数为1.89个,平均根长为6.28 cm,最大根长为7.63 cm,根系效果指数为0.60。在插穗粗度试验中以细插穗(0.15~0.25 cm)生根率最高,在泥炭土基质中可达55.0%,根系效果指数为0.52;中等插穗(0.25~0.35 cm)生根质量最高,生根率为30%,根系效果指数为0.75。[结论]使用泥炭土基质和粗度为0.15~0.25 cm的细插穗能促进醉香含笑扦插生根。
[Objective] To screen the optimal growth substrate and cutting diameter of Michelia macclurei Dandy,and to provide certain theoretical foundation for the industrialization development. [Method] With new terminal branches of M. macclurei as the propagating material,we researched the effects of 11 growth substrates and 3 cutting diameters on the cutting rooting of M. macclurei.[Result]Growth substrate and cutting diameter showed significant impacts on the cutting rooting of M. macclurei. Among the 11 substrates,peat soil was the best. In this substrate,the callus rate was 65. 0%; the rooting rate was 28. 3%; the average root number,the average root length,the maximum root length and root effect index were 1. 89,6. 28 cm,7. 63 cm and 0. 60,respectively. The rooting rate of thin cutting( 0. 15-0. 25 cm) was the highest,which reached55% in the peat soil,with the rooting effect index being 0. 52. The substrate cutting( 0. 25-0. 35) had the highest rooting quality,the rooting rate was 30. 0%,and the rooting effect index was 0. 75.[Conclusion] Using peat soil as the substrate and 0. 15-0. 25 cm cutting diameter promotes the cutting rooting of M. macclurei.
[Objective] To screen the optimal growth substrate and cutting diameter of Michelia macclurei Dandy,and to provide certain theoretical foundation for the industrialization development. [Method] With new terminal branches of M. macclurei as the propagating material,we researched the effects of 11 growth substrates and 3 cutting diameters on the cutting rooting of M. macclurei.[Result]Growth substrate and cutting diameter showed significant impacts on the cutting rooting of M. macclurei. Among the 11 substrates,peat soil was the best. In this substrate,the callus rate was 65. 0%; the rooting rate was 28. 3%; the average root number,the average root length,the maximum root length and root effect index were 1. 89,6. 28 cm,7. 63 cm and 0. 60,respectively. The rooting rate of thin cutting( 0. 15-0. 25 cm) was the highest,which reached55% in the peat soil,with the rooting effect index being 0. 52. The substrate cutting( 0. 25-0. 35) had the highest rooting quality,the rooting rate was 30. 0%,and the rooting effect index was 0. 75.[Conclusion] Using peat soil as the substrate and 0. 15-0. 25 cm cutting diameter promotes the cutting rooting of M. macclurei.
引文
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[15]BASHIR M A,ANJUM M A,CHAUDHRY Z,et al.Response of Jojoba(Simmondsia chinensis)cuttings to various concentrations of auxins[J].Belgian journal of botany,2009,41(6):2831-2840.
[2]林珠莲.防火路营建火力楠防火林带的效能研究[J].青海农林科技,2006(3):21-23,72.
[3]PAYNE R W,MURRAY D A,HARDING S A,et al.Gen stat[M].13th edition.UK:VSN International,Hemel Hempstead,Hertfordshire HP1 1ES,2010.
[4]黄儒珠,檀东飞,郑娅珊,等.醉香含笑叶挥发油化学成分[J].热带亚热带植物学报,2009,17(4):406-408.
[5]马惠芬,司马永康,郝佳波,等.多脉含笑和醉香含笑挥发油的化学成分研究[J].广东农业科学,2011,38(23):110-113.
[6]曹光球,林思祖,王爱萍,等.火力楠根超临界提取物对马尾松种子的化感效应研究[J].中国生态农业学报,2005,13(4):32-35.
[7]宋晓凯.醉香含笑根皮挥发性成分的GC-MS分析及对NIH3T3细胞生长抑制作用[J].中国现代应用药学,2011,28(12):1122-1125.
[8]宋晓凯,陆春良,胡堃,等.醉香含笑树皮挥发性成分GC-MS分析及其对Hep G2细胞体外生长抑制作用[J].中草药,2011,42(11):2213-2215.
[9]刘举,陈继富.木兰科四种植物种子油的提取及脂肪酸成分分析[J].广西植物,2013,33(2):208-213.
[10]王里,彭映辉,仲崇禄,等.火力楠14个种源种子形态变异分析[J].种子,2015,34(4):86-89.
[11]黄志玲,申文辉,梁一萍,等.一种火力楠扦插繁殖方法:中国,201410024539.0[P].1989-07-26.
[12]李雪,王淑芬,蒋雄辉.醉香含笑的组织培养与植株再生[J].植物生理学通讯,2005,41(6):783.
[13]BENTZER B G.Rooting and early shoot characteristics of Picea abies(L.)Karst cutting originating from shoots with enforced vertical growth Scand[J].For Res,1988(3):481-491.
[14]ABU-ZAHRA T R,HASAN M K,HASAN H S.Effect of different auxin concentrations on Virginia creeper(Parthenocissus quinquefolia)rooting[J].World applied sciences journal,2012,16(1):7-10.
[15]BASHIR M A,ANJUM M A,CHAUDHRY Z,et al.Response of Jojoba(Simmondsia chinensis)cuttings to various concentrations of auxins[J].Belgian journal of botany,2009,41(6):2831-2840.