慢性牙周炎和IgA肾病大鼠模型的建立及其相关性初步分析
|
摘要
目的:通过建立大鼠慢性牙周炎和IgA肾病复合模型,利用牙周指标如龈出血指数、探诊深度及附着丧失和肾脏病理染色指标等初步探讨慢性牙周炎和IgA肾病之间的关系。方法:选择SD大鼠,通过结扎和细菌感染方法建立牙周炎模型,灌胃、皮下注射等方法建立IgA肾病模型,12周末检测大鼠牙周指标、肾脏临床及病理指标,进行统计学分析。结果:通过牙周指标和肾脏指标,确认成功建立所需的动物模型,尤其复合模型组的牙周指标较牙周炎组更高(P<0. 001),同时复合模型组的肾脏病理评分和单纯肾病组相比较高(P<0. 05)。结论:慢性牙周炎和IgA肾病之间存在一定的联系,可能会互相影响发生发展。
Objective: By establishing a compound model of chronic periodontitis and Immunoglobulin A nephropathy in rats, the relationship between chronic periodontitis and immunoglobulin A nephropathy was studied by using periodontal indexes such as gingival bleeding index, probe depth, attachment loss and renal pathological staining. Methods: SpragueDawley rats was selected to establish periodontitis model by ligation and bacterial infection. The immunoglobulin A nephropathy model was established by intragastric administration and subcutaneous injection. Periodontal indexes, renal clinical and pathological indexes were detected and analyzed statistically at the end of 12 weeks. Results: The animal model was established successfully by periodontal index and renal index, especially the periodontal index in the compound model group was higher than that in the periodontitis group(P<0.001), and the renal pathological score in the composite model group was higher than that in the simple nephropathy group(P<0.05). Conclusion: There is a relationship between chronic periodontitis and immunoglobulin A nephropathy, which may affect the development of each other.
Objective: By establishing a compound model of chronic periodontitis and Immunoglobulin A nephropathy in rats, the relationship between chronic periodontitis and immunoglobulin A nephropathy was studied by using periodontal indexes such as gingival bleeding index, probe depth, attachment loss and renal pathological staining. Methods: SpragueDawley rats was selected to establish periodontitis model by ligation and bacterial infection. The immunoglobulin A nephropathy model was established by intragastric administration and subcutaneous injection. Periodontal indexes, renal clinical and pathological indexes were detected and analyzed statistically at the end of 12 weeks. Results: The animal model was established successfully by periodontal index and renal index, especially the periodontal index in the compound model group was higher than that in the periodontitis group(P<0.001), and the renal pathological score in the composite model group was higher than that in the simple nephropathy group(P<0.05). Conclusion: There is a relationship between chronic periodontitis and immunoglobulin A nephropathy, which may affect the development of each other.
引文
[1] Craandijk J, van Kru gten MV, Verw eij CL, et al. Tumornecrosis factor-alph a gene polymorph isms in relation to pe-riodontitis[J]. J Clin Periodontol,2002,29:28-34
[2] Briggs JE, McKeow n PP, Craw fod VL, et al. Angiograph i-cally confirmed coronary h eart disease and periodontal diseasein middle-aged males[J]. J Periodontol, 2006, 77:95-102
[3] Ksh irsagar AV, Moss KL, Elter JR, et al. Periodontal diseaseis associated w ith renal insufficiency in th e Ath erosclerosisRisk In Communities(ARIC)study[J]. Am J Kidney Dis,2005, 45:650-657
[4] Grubbs V, Plantinga L C, Crew s D C, et al. Vulnerable popu-lations and th e association betw een periodontal and ch ronickidney disease[J]. Clinical journal of th e American Society ofNeph rology:CJASN, 2011, 6(4):711-717
[5] Ioannidou E, H all Y, Sw ede H, et al. Periodontitis associatedw ith ch ronic kidney disease among Mex ican Americans[J].Journal of public h ealth dentistry, 2013, 73(2):112-119
[6] Piccolo M, De Angelis M, Lauriero G, et al. Salivarymicro-biota associated w ith immunoglobulin a neph ropath y[J]. Mi-crob Ecol, 2015, 70(2):557-565
[7]李静,张静等.慢性牙周炎与IgA肾病复合动物模型的建立及相互关系[J].中华肾脏病杂志,2013,29:842-848
[8] Raij L, Azar S, Keane W. Mesangial immune injury, h yper-tension and progressive glomerular damage in Dah l rats[J].Kidney Int, 1984, 26:137-143
[9] Ots M, Mackenzie H S, Troy JL, et al. Effects of combinationth erapy w ith enalapril and losartan on th e rate of progressionof renal injury in rats w ith 5/6 renal mass ablation[J]. J AmSoc Neph rol, 1998, 9:224-230
[10]王丽娟,缪羽,等.牙周炎与冠心病相关性的研究进展[J].中华老年口腔医学杂志, 2013, 11(1):47-50
[11] Kapellas K, Singh A, Bertotti M, et al. Periodontal andch ronic kidney disease association:a systematic review andmeta-analysis[J]. Neph rology, 2018
[12] Bayraktar G, Kurtulus I, et al. Dental and periodontal find-ings in h emodialysis patients[J]. Oral diseases, 2007, 13(4):393-397
[13]刘刚,马序竹,等.肾活检患者肾脏病构成十年对比分析[J].临床内科杂志,2004,21(12):834-838
[14] D'Amico G. Natural h istory of idiopath ic IgA neph ropath y:role of clinical and h istological prognostic factors[J]. Am JKidney Dis, 2000, 36:227-237
[15] Wyatt R J, H ogg R J. A randomized controlled trial of my-coph enolate mofetil in patients w ith IgA neph ropath y[IS-RCTN62574616][J]. BMC Neph rology, 5, 1(2004-03-25),2004, 5(1):1-9
[16] Fish er MA, Taylor GW, et al. Clinical and serologic markersof periodontal infection and ch ronic kidney disease[J]. J Peri-odontol, 2008, 79(9):1670-1678
[17] Papapanou PN, Neiderud AM, Sandros J, et al. Ch ecker-board assessments of serum antibodies to oral microbiota assurrogate markers of clinical periodontal status[J]. J Clin Pe-riodontol, 2001, 28(1):103-106
[18] TORRES S A, ROSA O P, H AYACIBARA M F, et al. Peri-odontal parameters and BANA test in patients w ith ch ronicrenal failure undergoing h emodialysis[J]. Journal of appliedoral science:revista FOB, 2010, 18(3):297-302
[19] VILELA E M, BASTOS J A, FERNANDES N, et al. Treat-ment of ch ronic periodontitis decreases serum proh epcidinlevels in patients w ith ch ronic kidney disease[J]. Clinics,2011, 66(4):657-62
[20] CRAIG R G, BOYLAN R, YIP J, et al. Serum IgG antibodyresponse to periodontal path ogens in minority populations:relationsh ip to periodontal disease status and progression[J]. JPeriodontal Res, 2002, 37(2):132-46
[21] Beck J D, Pankow J, Tyroler H A, et al. Dental infectionsand ath erosclerosis[J]. American H eart Journal, 1999, 138(5Pt 2):S528
[22] Ch ambrone L, Foz AM, Guglielmetti MR, et al. Periodonti-tis and ch ronic kidney disease:a systematic review of th e as-sociation of diseases and th e effect of periodontal treatment onestimated glomerular filtration rate[J]. J Clin Periodontol,2013, 40(5):443-456
[2] Briggs JE, McKeow n PP, Craw fod VL, et al. Angiograph i-cally confirmed coronary h eart disease and periodontal diseasein middle-aged males[J]. J Periodontol, 2006, 77:95-102
[3] Ksh irsagar AV, Moss KL, Elter JR, et al. Periodontal diseaseis associated w ith renal insufficiency in th e Ath erosclerosisRisk In Communities(ARIC)study[J]. Am J Kidney Dis,2005, 45:650-657
[4] Grubbs V, Plantinga L C, Crew s D C, et al. Vulnerable popu-lations and th e association betw een periodontal and ch ronickidney disease[J]. Clinical journal of th e American Society ofNeph rology:CJASN, 2011, 6(4):711-717
[5] Ioannidou E, H all Y, Sw ede H, et al. Periodontitis associatedw ith ch ronic kidney disease among Mex ican Americans[J].Journal of public h ealth dentistry, 2013, 73(2):112-119
[6] Piccolo M, De Angelis M, Lauriero G, et al. Salivarymicro-biota associated w ith immunoglobulin a neph ropath y[J]. Mi-crob Ecol, 2015, 70(2):557-565
[7]李静,张静等.慢性牙周炎与IgA肾病复合动物模型的建立及相互关系[J].中华肾脏病杂志,2013,29:842-848
[8] Raij L, Azar S, Keane W. Mesangial immune injury, h yper-tension and progressive glomerular damage in Dah l rats[J].Kidney Int, 1984, 26:137-143
[9] Ots M, Mackenzie H S, Troy JL, et al. Effects of combinationth erapy w ith enalapril and losartan on th e rate of progressionof renal injury in rats w ith 5/6 renal mass ablation[J]. J AmSoc Neph rol, 1998, 9:224-230
[10]王丽娟,缪羽,等.牙周炎与冠心病相关性的研究进展[J].中华老年口腔医学杂志, 2013, 11(1):47-50
[11] Kapellas K, Singh A, Bertotti M, et al. Periodontal andch ronic kidney disease association:a systematic review andmeta-analysis[J]. Neph rology, 2018
[12] Bayraktar G, Kurtulus I, et al. Dental and periodontal find-ings in h emodialysis patients[J]. Oral diseases, 2007, 13(4):393-397
[13]刘刚,马序竹,等.肾活检患者肾脏病构成十年对比分析[J].临床内科杂志,2004,21(12):834-838
[14] D'Amico G. Natural h istory of idiopath ic IgA neph ropath y:role of clinical and h istological prognostic factors[J]. Am JKidney Dis, 2000, 36:227-237
[15] Wyatt R J, H ogg R J. A randomized controlled trial of my-coph enolate mofetil in patients w ith IgA neph ropath y[IS-RCTN62574616][J]. BMC Neph rology, 5, 1(2004-03-25),2004, 5(1):1-9
[16] Fish er MA, Taylor GW, et al. Clinical and serologic markersof periodontal infection and ch ronic kidney disease[J]. J Peri-odontol, 2008, 79(9):1670-1678
[17] Papapanou PN, Neiderud AM, Sandros J, et al. Ch ecker-board assessments of serum antibodies to oral microbiota assurrogate markers of clinical periodontal status[J]. J Clin Pe-riodontol, 2001, 28(1):103-106
[18] TORRES S A, ROSA O P, H AYACIBARA M F, et al. Peri-odontal parameters and BANA test in patients w ith ch ronicrenal failure undergoing h emodialysis[J]. Journal of appliedoral science:revista FOB, 2010, 18(3):297-302
[19] VILELA E M, BASTOS J A, FERNANDES N, et al. Treat-ment of ch ronic periodontitis decreases serum proh epcidinlevels in patients w ith ch ronic kidney disease[J]. Clinics,2011, 66(4):657-62
[20] CRAIG R G, BOYLAN R, YIP J, et al. Serum IgG antibodyresponse to periodontal path ogens in minority populations:relationsh ip to periodontal disease status and progression[J]. JPeriodontal Res, 2002, 37(2):132-46
[21] Beck J D, Pankow J, Tyroler H A, et al. Dental infectionsand ath erosclerosis[J]. American H eart Journal, 1999, 138(5Pt 2):S528
[22] Ch ambrone L, Foz AM, Guglielmetti MR, et al. Periodonti-tis and ch ronic kidney disease:a systematic review of th e as-sociation of diseases and th e effect of periodontal treatment onestimated glomerular filtration rate[J]. J Clin Periodontol,2013, 40(5):443-456