摘要
目的:建立高效液相色谱(high performance liquid chromatography,HPLC)法同时测定番荔枝果实3个部位中13种酚类成分的种类及含量,并分析含量与抗氧化活性的相关性。方法:采用福林-酚法测定总酚含量;通过HPLC法对番荔枝果实中的单酚成分进行比较;通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基与2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid),ABTS)阳离子自由基清除能力的检测方法测定番荔枝果实的抗氧化活性。结果:番荔枝果实中总酚含量为果皮>果肉>种子,果皮含量最高为59.48~127.84 mg/g;番荔枝单酚含量结果表明果皮显著高于果肉和种子部位,各产地酚类种类和含量差异显著。果皮和果肉中对羟基苯甲酸、儿茶素、咖啡酸、表儿茶素含量相对较高,表儿茶素最高为2 398.17 mg/mL。抗氧化结果表明番荔枝果实清除ABTS阳离子自由基能力高于DPPH自由基,且果皮活性最好,分别在质量浓度为0.39、1.56 mg/m L时均达到最高清除率。结论:番荔枝果实中果皮、果肉、种子3个部位中所含酚类物质种类及含量有显著性差异;果皮部位所含种类及含量均最高,且抗氧化活性最佳,可作为天然抗氧化剂。
Objective: To establish a high performance liquid chromatographic(HPLC) method for the simultaneous determination of the contents of 13 phenolic constituents in three fruit parts of Annona squamosa Linn., and to analyze the correlation between phenol content and antioxidant activity. Methods: Total phenols were evaluated by a colorimetric method, and the HPLC method was used to measure the composition and contents of phenols. The antioxidant activity was analyzed using the 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical cation scavenging activity assays. Results: The total phenol content of peel was the highest, ranging from 59.48 to127.84 mg/g, sequentially followed by pulp and seeds. The contents of some individual phenolic compounds in peel significantly higher than in any other parts. There were significant regional differences in the composition and contents of phenols in the same fruit parts. The contents of p-hydroxybenzoic acid, catechin, caffeic acid and(-)-epicatechin were relatively higher in peel and pulp and the highest(-)-epicatechin level was 2 398.17 mg/mL. All of the fruit parts were had more potent scavenging activity against DPPH radical than against ABTS radical cation and peel had the strongest radical scavenging with the highest percentage inhibition of DPPH radical and ABTS radical cation being observed at concentrations of 0.39 and 1.56 mg/mL, respectively. Conclusion: The composition and contents of phenols in the peel, pulp and seeds of A. squamosa fruit are significantly different. The peel contains the highest number and amount of phenols and possesses the highest antioxidant activity, indicating that it can be considered as a potential and valuable source of natural antioxidants.
引文
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