HPLC法同时测定金鸡虎补丸中的去氢二异丁香酚、五味子甲素、染料木素和芒柄花素
|
摘要
目的建立HPLC法同时测定金鸡虎补丸中去氢二异丁香酚、五味子甲素、染料木素和芒柄花素4种成分。方法金鸡虎补丸70%乙醇提取液的分析采用C18色谱柱(200mm×4.6mm,5μm);流动相A为乙腈-甲醇(3∶2),流动相B为0.1%H3PO4溶液,梯度洗脱;体积流量为0.9mL/min;去氢二异丁香酚检测波长为270nm,五味子甲素、染料木素和芒柄花素均为254nm;柱温为35℃;进样量20μL。结果被测定的4组成分色谱峰均有良好的分离度,方法精密度、重复性的RSD均小于2.0%;在室温条件下24h内稳定;各组分线性范围较宽,线性关系良好(r≥0.999 4);平均加样回收率为96.8%~98.1%,RSD(n=6)≤1.4%。结论金鸡虎补丸的提取方法和流动相保证了分析方法的简单易行、重复性好、准确可靠。
AIM To develop an HPLC method for simultaneously determining four components,such as dehydrodiisoeugenol,deoxyschizandrin,genistein and formononetin in Jinji Hubu Pills.METHODS The chromatograhic separation of 70% ethanolic extract of Jinji Hubu Pills was performed on C18column( 200mm × 4.6mm,5 μm).Mobile phase consisted of acetonitrile-methanol( 3 ∶ 2)( A) and 0.1% phosphoric acid solution( B)were adopted at the flow rate of 0.9mL min.The detection wavelengths were 270nm for dehydrodiisoeugenol,254 nm for deoxyschizandrin,genistein and formononetin,respectively.The column temperature was 35 ℃.The injection volume was 20 μL.RESULTS The four compounds were well separated.The RSD values of precision and reproducibility were all less than 2%.The stability was good in 24h.The linear relationship between the concentration and peak areas of the four compounds was all greater than 0.999 4.The recoveries were 96.8%- 98.1%and RSD( n = 6) less than 1.4%.CONCLUSION The developed extraction and mobile phase were simple,sensitive,accurate,and reproducible for Jinji Hubu Pills.
AIM To develop an HPLC method for simultaneously determining four components,such as dehydrodiisoeugenol,deoxyschizandrin,genistein and formononetin in Jinji Hubu Pills.METHODS The chromatograhic separation of 70% ethanolic extract of Jinji Hubu Pills was performed on C18column( 200mm × 4.6mm,5 μm).Mobile phase consisted of acetonitrile-methanol( 3 ∶ 2)( A) and 0.1% phosphoric acid solution( B)were adopted at the flow rate of 0.9mL min.The detection wavelengths were 270nm for dehydrodiisoeugenol,254 nm for deoxyschizandrin,genistein and formononetin,respectively.The column temperature was 35 ℃.The injection volume was 20 μL.RESULTS The four compounds were well separated.The RSD values of precision and reproducibility were all less than 2%.The stability was good in 24h.The linear relationship between the concentration and peak areas of the four compounds was all greater than 0.999 4.The recoveries were 96.8%- 98.1%and RSD( n = 6) less than 1.4%.CONCLUSION The developed extraction and mobile phase were simple,sensitive,accurate,and reproducible for Jinji Hubu Pills.
引文
[1]国家药典委员会.国家药品标准中药成方制剂第一册[S].北京:人民卫生出版社,2004:98.
[2]国家药典委员会.中华人民共和国药典:2010年版一部[S].北京:中国医药科技出版社,2010:180,附录30,附录36.
[3]王楠,李占林,刘晓秋,等.黑老虎根化学成分的研究(Ⅱ)[J].中国药物化学杂志,2012,22(4):305-309.
[4]余燕影,章丽华,曹树稳,等.RP-HPLC法测定丰城鸡血藤提取物中大豆黄酮、染料木素、刺芒柄花素和美皂异黄酮[J].中草药,2006,37(11):1655-1656.
[5]黄莉,张洪,李华荣,等.RP-HPLC法测定鸡血藤合剂中芒柄花素的含量[J].中医药导报,2013,19(9):79-80.
[6]郭希庆.鸡血藤糖浆中芒柄花素含量的高效液相色谱法测定[J].时珍国医国药,2013,24(11):2657-2658.
[7]周会芹,郑灏,支荣荣,等.HPLC法测定抗骨增生片中芒柄花素的含量[J].首都医药,2013,20(16):75-76.
[8]李莹,陈晓辉,张天虹,等.RP-HPLC法测定鸡血藤中芒柄花素的含量[J].沈阳药科大学学报,2009,26(12):975-977.
[9]马强,周玉新,雷海民,等.HPLC测定红车轴草中大豆苷元、染料木素、芒柄花素和鹰嘴豆芽素A的含量[J].中成药,2005,27(10):1193-1195.
[10]尤春雪,张振秋,侯学智,等.HPLC波长切换技术同时测定升麻、葛根药对提取物中11个成分[J].中成药,2013,35(4):761-765.
[11]吴梅,阿萍,周雪杉,等.二十味肉豆蔻丸质量标准研究[J].中国实验方剂学杂志,2013,19(19):111-114.
[12]刘晓哲.多波长RP-HPLC法同时测定肥儿丸中木香烃内酯、去氢木香内酯、去氢二异丁香酚[J].中成药,2014,36(1):98-101.
[13]张林林,王永吉,李航,等.UFLC法同时测定护肝胶囊中五味子醇甲、五味子甲素、五味子乙素[J].中成药,2013,35(1):96-99.
[14]梁永枢,安冉,刘军民,等.不同产地鸡血藤药材中染料木素及芒柄花素的含量测定[J].时珍国医国药,2013,24(7):1655-1657.
[15]章运典,周里欣,薛素娟,等.RP-HPLC法测定脑力宝丸中5种成分[J].中成药,2014,36(4):776-779.
[2]国家药典委员会.中华人民共和国药典:2010年版一部[S].北京:中国医药科技出版社,2010:180,附录30,附录36.
[3]王楠,李占林,刘晓秋,等.黑老虎根化学成分的研究(Ⅱ)[J].中国药物化学杂志,2012,22(4):305-309.
[4]余燕影,章丽华,曹树稳,等.RP-HPLC法测定丰城鸡血藤提取物中大豆黄酮、染料木素、刺芒柄花素和美皂异黄酮[J].中草药,2006,37(11):1655-1656.
[5]黄莉,张洪,李华荣,等.RP-HPLC法测定鸡血藤合剂中芒柄花素的含量[J].中医药导报,2013,19(9):79-80.
[6]郭希庆.鸡血藤糖浆中芒柄花素含量的高效液相色谱法测定[J].时珍国医国药,2013,24(11):2657-2658.
[7]周会芹,郑灏,支荣荣,等.HPLC法测定抗骨增生片中芒柄花素的含量[J].首都医药,2013,20(16):75-76.
[8]李莹,陈晓辉,张天虹,等.RP-HPLC法测定鸡血藤中芒柄花素的含量[J].沈阳药科大学学报,2009,26(12):975-977.
[9]马强,周玉新,雷海民,等.HPLC测定红车轴草中大豆苷元、染料木素、芒柄花素和鹰嘴豆芽素A的含量[J].中成药,2005,27(10):1193-1195.
[10]尤春雪,张振秋,侯学智,等.HPLC波长切换技术同时测定升麻、葛根药对提取物中11个成分[J].中成药,2013,35(4):761-765.
[11]吴梅,阿萍,周雪杉,等.二十味肉豆蔻丸质量标准研究[J].中国实验方剂学杂志,2013,19(19):111-114.
[12]刘晓哲.多波长RP-HPLC法同时测定肥儿丸中木香烃内酯、去氢木香内酯、去氢二异丁香酚[J].中成药,2014,36(1):98-101.
[13]张林林,王永吉,李航,等.UFLC法同时测定护肝胶囊中五味子醇甲、五味子甲素、五味子乙素[J].中成药,2013,35(1):96-99.
[14]梁永枢,安冉,刘军民,等.不同产地鸡血藤药材中染料木素及芒柄花素的含量测定[J].时珍国医国药,2013,24(7):1655-1657.
[15]章运典,周里欣,薛素娟,等.RP-HPLC法测定脑力宝丸中5种成分[J].中成药,2014,36(4):776-779.