辽河口芦苇湿地细菌和古菌群落周期日变化特征
|
摘要
采用实时荧光定量PCR和Illumina高通量测序技术对秋季辽河口芦苇湿地水体中一昼夜细菌和古菌群落特征进行了研究.测序共获得52802条细菌和106091条古菌高质量序列,以97%的相似性聚类并抽平后共划分了530和979个OTU.结果表明,湿地水体中浮游细菌和古菌群落丰度均在早上达到最高值,夜晚达到最低值.细菌和古菌群落多样性指数均存在周期日变化,不同时间细菌和古菌群落结构和组成具有显著差异.此外,古菌群落组成特征表明辽河口芦苇湿地具有较高的潜在甲烷生成能力.本研究为进一步认识河口湿地细菌和古菌群落特征和功能奠定了基础.
The present study investigated the diurnal variations of bacterial and archaeal communities in Liaohe estuarine wetland inautumn, using the real-time fluorescence quantitative PCR method and Illumina high-throughput sequencing. The results showed that52802 bacterial and 106091 archaeal sequences were obtained, which could be classified into 530 and 979 OTUs in 97% similaritylevel, respectively. The results showed that bacterial and archaeal 16 S r RNA gene abundances both had the highest values in themorning, and the lowest ones at midnight. There were diurnal variations in the bacterial and archaeal community α-diversity indices,and obvious temporal changes were also observed in the bacterial and archaeal community compositions and structures. In addition,Liaohe estuarine wetland had high potential ability of methanogenesis which could be reflected by the composition of archaealcommunity. This study provides the basic information and knowledge to further understand the characteristics and functions ofbacterial and archaeal communities in estuarine wetlands.
The present study investigated the diurnal variations of bacterial and archaeal communities in Liaohe estuarine wetland inautumn, using the real-time fluorescence quantitative PCR method and Illumina high-throughput sequencing. The results showed that52802 bacterial and 106091 archaeal sequences were obtained, which could be classified into 530 and 979 OTUs in 97% similaritylevel, respectively. The results showed that bacterial and archaeal 16 S r RNA gene abundances both had the highest values in themorning, and the lowest ones at midnight. There were diurnal variations in the bacterial and archaeal community α-diversity indices,and obvious temporal changes were also observed in the bacterial and archaeal community compositions and structures. In addition,Liaohe estuarine wetland had high potential ability of methanogenesis which could be reflected by the composition of archaealcommunity. This study provides the basic information and knowledge to further understand the characteristics and functions ofbacterial and archaeal communities in estuarine wetlands.
引文
[1]杨朝飞.中国湿地现状及其保护对策[J].中国环境科学,1995,15(6):407-412.Yang C F.Current status and conservation strategy of China’s wetland[J].China Environmental Science,1995,15(6):407-412.
[2]黄桂林,何平,侯盟.中国河口湿地研究现状及展望[J].应用生态学报,2006,17(9):1751-1756.Huang G L,He P,Hou M.Present status and prospects of estuarine wetland research in China[J].Chinese Journal of Applied Ecology,2006,17(9):1751-1756.
[3]车玉伶,王慧,胡洪营,等.微生物群落结构和多样性解析技术研究进展[J].生态环境学报,2005,14(1):127-133.Che Y L,Wang H,Hu H Y,et al.Research progresses on analytical technologies used in microbial community structure and diversity[J].Ecology and Environment,2005,14(1):127-133.
[4]Franklin R B,Morrissey E Mand Morina J C.Changes in abundance and community structure of nitrate-reducing bacteria along a salinity gradient in tidal wetlands[J].Pedobiologia,2017,60:21-26.
[5]Zhang H,Zheng S,Ding J,et al.Spatial variation in bacterial community in natural wetland-river-sea ecosystems[J].Journal of Basic Microbiology,2017,57(6):536-546.
[6]Li X,Hou L,Liu M,et al.Primary effects of extracellular enzyme activity and microbial community on carbon and nitrogen mineralization in estuarine and tidal wetlands[J].Applied Microbiology&Biotechnology,2015,99(6):2895-2909.
[7]Fei X X,Wang L,Jun H J,et al.Salinity influence on soil microbial respiration rate of wetland in the Yangtze River estuary through changing microbial community[J].Journal of Environmental Science,2014,26(12):2562-2570.
[8]Zhang Y,Wang L,Hu Y,et al.Water Organic Pollution and Eutrophication Influence Soil Microbial Processes,Increasing Soil Respiration of Estuarine Wetlands:Site Study in Jiuduansha Wetland[J].Plos One,2015,10(5):e0126951.
[9]Lin T,Ye S,Ma C,et al.Sources and preservation of organic matter in soils of the wetlands in the Liaohe(Liao River)Delta,North China[J].Marine Pollution Bulletin,2013,71(1/2):276-285.
[10]关晓燕,韩家波,王摆,等.辽东湾大凌河口湿地土壤微生物群落分析[J].生态环境学报,2012,21(6):1063-1070.Guan X Y,Han J P,Wang B,et al.Analysis of bacterial communities in Liaodong Bay Dalinghe estuarine wetland[J].Ecology and Environmental Sciences,2012,21(6):1063-1070.
[11]赵先丽,周广胜,周莉,等.盘锦芦苇湿地土壤微生物数量研究[J].土壤通报,2008,39(6):1376-1379.Zhao X L,Zhou G S,Zhou L,et al.Characteristics of soil microbial community in bulrush wetlands of Panjin,Northeast China[J].Chinese Journal of Soil Science,2008,39(6):1376-1379.
[12]鲁青原.辽河三角洲滨海湿地微生物群落组成及其环境意义[D].北京:中国地质大学,2016.Lu Q Y.The microbial community structure in Liaohe Delta wetlands and its environmental significances[D].Beijing:China University of Geosciences,2016.
[13]田伟君,王勇梅,孙会梅,等.石油输入对河口芦苇湿地根际微生物的影响[J].中国环境科学,2014,34(10):2676-2683.Tian W J,Wang Y M,Sun H M,et al.Effect of oil pollution on community structure of plants rhizospheric microorganisms in estuarine wetland[J].China Environmental Science,2014,34(10):2676-2683.
[14]李璐,邹立,孟泰舟,等.辽河口芦苇湿地有色溶解有机物的光谱特征研究[J].中国海洋大学学报:自然科学版,2017,(12):27-36.Li L,Zou L,Meng T Z,et al.Absorbance and fluorescence spectrums of chromophoric dissolved organic matter in the reed wetland of Liaohe estuary[J].Periodical of Ocean University of China,2017,(12):27-36.
[15]Peiffer J A,Spor A,Koren O,et al.Diversity and heritability of the maize rhizosphere microbiome under field conditions[J].Proceedings of the National Academy of Sciences,2013,110(16):6548-6553.
[16]Porat I,Vishnivetskaya T A,Mosher J J,et al.Characterization of archaeal community in contaminated and uncontaminated surface stream sediments[J].Microbial ecology,2010,60(4):784-795.
[17]Carlos,Nossa,William,et al.Design of 16S r RNA gene primers for454pyrosequencing of the human foregut microbiome[J].World Journal of Gastroenterology,2010,16(33):4135-4144.
[18]Ohene-Adjei S,Teather R M,Ivan M,et al.Postinoculation protozoan establishment and association patterns of methanogenic archaea in the ovine rumen[J].Applied and Environmental Microbiology,2007,73(14):4609-4618.
[19]Bolger A M,Lohse Mand Usadel B.Trimmomatic:a flexible trimmer for Illumina sequence data[J].Bioinformatics,2014,30(15):2114-2120.
[20]Reyon D,Tsai S Q,Khayter C,et al.FLASH assembly of TALENs for high-throughput genome editing[J].Nature Biotechnology,2012,30(5):460-465.
[21]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[22]Rognes T,Flouri T,Nichols B,et al.VSEARCH:a versatile open source tool for metagenomics[J].Peerj,2016,4(10):1-22.
[23]Wang Q,Garrity G M,Tiedje J M,et al.Naive Bayesian classifier for rapid assignment of r RNA sequences into the new bacterial taxonomy[J].Applied&Environmental Microbiology,2007,73(16):5261-5267.
[24]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013,41(Database issue):590-596.
[25]Galand P E,Casamayor E O,Kirchman D L,et al.Ecology of the rare microbial biosphere of the Arctic Ocean[J].Proceedings of the National Academy of Sciences,2009,106(52):22427-22432.
[26]罗西玲,邢磊,徐宾铎,等.辽河口芦苇湿地河蟹养殖区水体N、P营养盐和COD的变化[J].海洋湖沼通报,2016,(3):20-27.Luo X L,Xing L,Xu B Y,et al.Variations in nitrogen and phosphorous nutrients and COD in the river crab aquaculture areas in reed wetland of Liaohe estuary[J].Transactions of Oceanology and Limnology,2016,(3):20-27.
[27]徐微雪,段亮,宋永会,等.辽河保护区七星湿地表层水与间隙水中氮的时空分布[J].环境工程技术学报,2014,4(1):40-45.Xu W X,Duan L,Song Y H,et al.Spatio-temporal distributions of nitrogen in surface water and pore water in Qixing Wetland of Liaohe conservation area[J].Journal of Environmental Engineering Technology,2014,4(1):40-45.
[28]吴明,邵学新,蒋科毅.西溪国家湿地公园水体和底泥N、P营养盐分布特征及评价[J].林业科学研究,2008,21(4):587-591.Wu M,Shao X X,Jiang K Y.Characteristics and assessment on nutrient distribution in water and sediments of Xixi National Wetland Park in Hangzhou[J].Forest Research,2008,21(4):587-591.
[29]唐玉姝,王磊,席雪飞,等.九段沙湿地潮间带水域有机碳与无机碳含量的空间分布特征[J].农业环境科学学报,2012,31(12):2459-2465.Tang Y S,Wang L,Xi X F,et al.The spatial distribution characteristics of organic and inorganic carbon content of intertidal water in Jiuduansha Wetland[J].Journal of Agro-Environment Science,2012,31(12):2459-2465.
[30]刘晓辉.长江口及邻近海域海水细菌多样性及时空分布规律研究[D].舟山:浙江海洋大学,2017.Liu X H.Temporal and spatial distribution of bacterial diversity in seawater of Changjiang estuary and adjacent areas[D].Zhoushan:Zhejiang Ocean University,2017.
[31]宋玉芝,赵淑颖,黄瑾,等.太湖水体附着细菌和浮游细菌的丰度与分布特征[J].环境工程学报,2013,7(8):2825-2831.Song Y Z,Zhao S Y,Huang J,et al.Abundance and distribution of particle-attached bacteria and free-living bacteria in Lake Taihu[J].Chinese Journal of Environmental Engineering,2013,7(8):2825-2831.
[32]Wang L,Zhang J,Li H,et al.Shift in the microbial community composition of surface water and sediment along an urban river[J].Science of the Total Environment,2018,627:600-612.
[33]Li J,Zhou H,Fang J,et al.Microbial distribution in different spatial positions within the walls of a black sulfide hydrothermal chimney[J].Marine Ecology Progress Series,2014,508:67-85.
[34]Yang Y,Dai Y,Wu Z,et al.Temporal and spatial dynamics of archaeal communities in two freshwater lakes at different trophic status[J].Frontiers in Microbiology,2016,7:451.
[35]Hu A,Hou Land Yu C-P.Biogeography of planktonic and benthic archaeal communities in a subtropical eutrophic estuary of China[J].Microbial Ecology,2015,70(2):322-335.
[36]崔尹赡,李珊,魏云林,等.纳帕海湿地浮游细菌的丰度及可培养低温细菌多样性研究[J].上海环境科学,2017,36(3):93-138.Cui Y S,Li S,Wei Y L,et al.Studies on the abundance of bacterioplankton and diversity of culturable low-temperature bacteria in Napahai Wetland[J].Shanghai Environmental Science,2017,36(3):93-138.
[37]李云,李道季,张利华,等.长江口极微型和微微型浮游生物的垂向变化与周日波动[J].海洋科学,2011,35(9):24-30.Li Y,Li D J,Zhang L H,et al.Vertical characteristics and diel variations of femtoplankton and picoplankton at the Changjiang estuary[J].Marine Science,2011,35(9):24-30.
[38]Barbosa A B,Galva?o H M,Mendes P A,et al.Short-term variability of heterotrophic bacterioplankton during upwelling off the NW Iberian margin[J].Progress in Oceanography,2001,51:339-359.
[39]Zhang R,Weinbauer M G,Qian P Y.Viruses and flagellates sustain apparent richness and reduce biomass accumulation of bacterioplankton in coastal marine waters[J].Environmental Microbiology,2007,9(12):3008-3018.
[40]Winter C,Herndl G J,Weinbauer M G.Diel cycles in viral infection of bacterioplankton in the North Sea[J].Aquatic Microbial Ecology,2004,35:207-216.
[41]Zhang J,Zhang X,Liu Y,et al.Bacterioplankton communities in a high-altitude freshwater wetland[J].Annals of Microbiology,2014,64(3):1405-1411.
[42]Elsayed O,Maillard E,Vuilleumier S,et al.Bacterial communities in batch and continuous-flow wetlands treating the herbicide S-metolachlor[J].Science of the Total Environment,2014,499:327-335.
[43]Zhang L,Gao G,Tang X,et al.Bacterial community changes along a salinity gradient in a Chinese wetland[J].Canadian Journal of Microbiology,2013,59(9):611-619.
[44]Baik K S,Park S C,Kim E M,et al.Diversity of bacterial community in freshwater of Woopo wetland[J].The Journal of Microbiology,2008,46(6):647-655.
[45]Buesing N,Filippini M,Bürgmann H,et al.Microbial communities in contrasting freshwater marsh microhabitats[J].FEMS Microbiology Ecology,2009,69(1):84-97.
[46]Wan T,Zhang G,Du F,et al.Combined biologic aerated filter and sulfur/ceramisite autotrophic denitrification for advanced wastewater nitrogen removal at low temperatures[J].Frontiers of Environmental Science&Engineering,2014,8(6):967-972.
[47]Huang X,Bai J,Li K R,et al.Characteristics of two novel cold-and salt-tolerant ammonia-oxidizing bacteria from Liaohe Estuarine Wetland[J].Marine Pollution Bulletin,2017,114(1):192-200.
[48]Li B,Chen H,Li N,et al.Spatio-temporal shifts in the archaeal community of a constructed wetland treating river water[J].Science of the Total Environment,2017,605:269-275.
[49]Long Y,Yi H,Chen S,et al.Influences of plant type on bacterial and archaeal communities in constructed wetland treating polluted river water[J].Environmental Science and Pollution Research,2016,23(19):19570-19579.
[50]Giovannoni S J,Stinql U.Molecular diversity and ecology of microbial plankton[J].Nature,2005,437(7057):343-348.
[51]Liu Y,Whitman W B.Metabolic,phylogenetic,and ecological diversity of the methanogenic archaea[J].Annals of the New York Academy of Sciences,2008,1125(1):171-189.
[52]张健,赵阳国,李海艳,等.黄海西北近岸沉积物中细菌群落空间分布特征[J].海洋学报,2010,32(2):118-127.Zhang J,Zhao Y G,Li H Y,et al.Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea[J].Acta Oceanologica Sinica,2010,32(2):118-127.
[2]黄桂林,何平,侯盟.中国河口湿地研究现状及展望[J].应用生态学报,2006,17(9):1751-1756.Huang G L,He P,Hou M.Present status and prospects of estuarine wetland research in China[J].Chinese Journal of Applied Ecology,2006,17(9):1751-1756.
[3]车玉伶,王慧,胡洪营,等.微生物群落结构和多样性解析技术研究进展[J].生态环境学报,2005,14(1):127-133.Che Y L,Wang H,Hu H Y,et al.Research progresses on analytical technologies used in microbial community structure and diversity[J].Ecology and Environment,2005,14(1):127-133.
[4]Franklin R B,Morrissey E Mand Morina J C.Changes in abundance and community structure of nitrate-reducing bacteria along a salinity gradient in tidal wetlands[J].Pedobiologia,2017,60:21-26.
[5]Zhang H,Zheng S,Ding J,et al.Spatial variation in bacterial community in natural wetland-river-sea ecosystems[J].Journal of Basic Microbiology,2017,57(6):536-546.
[6]Li X,Hou L,Liu M,et al.Primary effects of extracellular enzyme activity and microbial community on carbon and nitrogen mineralization in estuarine and tidal wetlands[J].Applied Microbiology&Biotechnology,2015,99(6):2895-2909.
[7]Fei X X,Wang L,Jun H J,et al.Salinity influence on soil microbial respiration rate of wetland in the Yangtze River estuary through changing microbial community[J].Journal of Environmental Science,2014,26(12):2562-2570.
[8]Zhang Y,Wang L,Hu Y,et al.Water Organic Pollution and Eutrophication Influence Soil Microbial Processes,Increasing Soil Respiration of Estuarine Wetlands:Site Study in Jiuduansha Wetland[J].Plos One,2015,10(5):e0126951.
[9]Lin T,Ye S,Ma C,et al.Sources and preservation of organic matter in soils of the wetlands in the Liaohe(Liao River)Delta,North China[J].Marine Pollution Bulletin,2013,71(1/2):276-285.
[10]关晓燕,韩家波,王摆,等.辽东湾大凌河口湿地土壤微生物群落分析[J].生态环境学报,2012,21(6):1063-1070.Guan X Y,Han J P,Wang B,et al.Analysis of bacterial communities in Liaodong Bay Dalinghe estuarine wetland[J].Ecology and Environmental Sciences,2012,21(6):1063-1070.
[11]赵先丽,周广胜,周莉,等.盘锦芦苇湿地土壤微生物数量研究[J].土壤通报,2008,39(6):1376-1379.Zhao X L,Zhou G S,Zhou L,et al.Characteristics of soil microbial community in bulrush wetlands of Panjin,Northeast China[J].Chinese Journal of Soil Science,2008,39(6):1376-1379.
[12]鲁青原.辽河三角洲滨海湿地微生物群落组成及其环境意义[D].北京:中国地质大学,2016.Lu Q Y.The microbial community structure in Liaohe Delta wetlands and its environmental significances[D].Beijing:China University of Geosciences,2016.
[13]田伟君,王勇梅,孙会梅,等.石油输入对河口芦苇湿地根际微生物的影响[J].中国环境科学,2014,34(10):2676-2683.Tian W J,Wang Y M,Sun H M,et al.Effect of oil pollution on community structure of plants rhizospheric microorganisms in estuarine wetland[J].China Environmental Science,2014,34(10):2676-2683.
[14]李璐,邹立,孟泰舟,等.辽河口芦苇湿地有色溶解有机物的光谱特征研究[J].中国海洋大学学报:自然科学版,2017,(12):27-36.Li L,Zou L,Meng T Z,et al.Absorbance and fluorescence spectrums of chromophoric dissolved organic matter in the reed wetland of Liaohe estuary[J].Periodical of Ocean University of China,2017,(12):27-36.
[15]Peiffer J A,Spor A,Koren O,et al.Diversity and heritability of the maize rhizosphere microbiome under field conditions[J].Proceedings of the National Academy of Sciences,2013,110(16):6548-6553.
[16]Porat I,Vishnivetskaya T A,Mosher J J,et al.Characterization of archaeal community in contaminated and uncontaminated surface stream sediments[J].Microbial ecology,2010,60(4):784-795.
[17]Carlos,Nossa,William,et al.Design of 16S r RNA gene primers for454pyrosequencing of the human foregut microbiome[J].World Journal of Gastroenterology,2010,16(33):4135-4144.
[18]Ohene-Adjei S,Teather R M,Ivan M,et al.Postinoculation protozoan establishment and association patterns of methanogenic archaea in the ovine rumen[J].Applied and Environmental Microbiology,2007,73(14):4609-4618.
[19]Bolger A M,Lohse Mand Usadel B.Trimmomatic:a flexible trimmer for Illumina sequence data[J].Bioinformatics,2014,30(15):2114-2120.
[20]Reyon D,Tsai S Q,Khayter C,et al.FLASH assembly of TALENs for high-throughput genome editing[J].Nature Biotechnology,2012,30(5):460-465.
[21]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[22]Rognes T,Flouri T,Nichols B,et al.VSEARCH:a versatile open source tool for metagenomics[J].Peerj,2016,4(10):1-22.
[23]Wang Q,Garrity G M,Tiedje J M,et al.Naive Bayesian classifier for rapid assignment of r RNA sequences into the new bacterial taxonomy[J].Applied&Environmental Microbiology,2007,73(16):5261-5267.
[24]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013,41(Database issue):590-596.
[25]Galand P E,Casamayor E O,Kirchman D L,et al.Ecology of the rare microbial biosphere of the Arctic Ocean[J].Proceedings of the National Academy of Sciences,2009,106(52):22427-22432.
[26]罗西玲,邢磊,徐宾铎,等.辽河口芦苇湿地河蟹养殖区水体N、P营养盐和COD的变化[J].海洋湖沼通报,2016,(3):20-27.Luo X L,Xing L,Xu B Y,et al.Variations in nitrogen and phosphorous nutrients and COD in the river crab aquaculture areas in reed wetland of Liaohe estuary[J].Transactions of Oceanology and Limnology,2016,(3):20-27.
[27]徐微雪,段亮,宋永会,等.辽河保护区七星湿地表层水与间隙水中氮的时空分布[J].环境工程技术学报,2014,4(1):40-45.Xu W X,Duan L,Song Y H,et al.Spatio-temporal distributions of nitrogen in surface water and pore water in Qixing Wetland of Liaohe conservation area[J].Journal of Environmental Engineering Technology,2014,4(1):40-45.
[28]吴明,邵学新,蒋科毅.西溪国家湿地公园水体和底泥N、P营养盐分布特征及评价[J].林业科学研究,2008,21(4):587-591.Wu M,Shao X X,Jiang K Y.Characteristics and assessment on nutrient distribution in water and sediments of Xixi National Wetland Park in Hangzhou[J].Forest Research,2008,21(4):587-591.
[29]唐玉姝,王磊,席雪飞,等.九段沙湿地潮间带水域有机碳与无机碳含量的空间分布特征[J].农业环境科学学报,2012,31(12):2459-2465.Tang Y S,Wang L,Xi X F,et al.The spatial distribution characteristics of organic and inorganic carbon content of intertidal water in Jiuduansha Wetland[J].Journal of Agro-Environment Science,2012,31(12):2459-2465.
[30]刘晓辉.长江口及邻近海域海水细菌多样性及时空分布规律研究[D].舟山:浙江海洋大学,2017.Liu X H.Temporal and spatial distribution of bacterial diversity in seawater of Changjiang estuary and adjacent areas[D].Zhoushan:Zhejiang Ocean University,2017.
[31]宋玉芝,赵淑颖,黄瑾,等.太湖水体附着细菌和浮游细菌的丰度与分布特征[J].环境工程学报,2013,7(8):2825-2831.Song Y Z,Zhao S Y,Huang J,et al.Abundance and distribution of particle-attached bacteria and free-living bacteria in Lake Taihu[J].Chinese Journal of Environmental Engineering,2013,7(8):2825-2831.
[32]Wang L,Zhang J,Li H,et al.Shift in the microbial community composition of surface water and sediment along an urban river[J].Science of the Total Environment,2018,627:600-612.
[33]Li J,Zhou H,Fang J,et al.Microbial distribution in different spatial positions within the walls of a black sulfide hydrothermal chimney[J].Marine Ecology Progress Series,2014,508:67-85.
[34]Yang Y,Dai Y,Wu Z,et al.Temporal and spatial dynamics of archaeal communities in two freshwater lakes at different trophic status[J].Frontiers in Microbiology,2016,7:451.
[35]Hu A,Hou Land Yu C-P.Biogeography of planktonic and benthic archaeal communities in a subtropical eutrophic estuary of China[J].Microbial Ecology,2015,70(2):322-335.
[36]崔尹赡,李珊,魏云林,等.纳帕海湿地浮游细菌的丰度及可培养低温细菌多样性研究[J].上海环境科学,2017,36(3):93-138.Cui Y S,Li S,Wei Y L,et al.Studies on the abundance of bacterioplankton and diversity of culturable low-temperature bacteria in Napahai Wetland[J].Shanghai Environmental Science,2017,36(3):93-138.
[37]李云,李道季,张利华,等.长江口极微型和微微型浮游生物的垂向变化与周日波动[J].海洋科学,2011,35(9):24-30.Li Y,Li D J,Zhang L H,et al.Vertical characteristics and diel variations of femtoplankton and picoplankton at the Changjiang estuary[J].Marine Science,2011,35(9):24-30.
[38]Barbosa A B,Galva?o H M,Mendes P A,et al.Short-term variability of heterotrophic bacterioplankton during upwelling off the NW Iberian margin[J].Progress in Oceanography,2001,51:339-359.
[39]Zhang R,Weinbauer M G,Qian P Y.Viruses and flagellates sustain apparent richness and reduce biomass accumulation of bacterioplankton in coastal marine waters[J].Environmental Microbiology,2007,9(12):3008-3018.
[40]Winter C,Herndl G J,Weinbauer M G.Diel cycles in viral infection of bacterioplankton in the North Sea[J].Aquatic Microbial Ecology,2004,35:207-216.
[41]Zhang J,Zhang X,Liu Y,et al.Bacterioplankton communities in a high-altitude freshwater wetland[J].Annals of Microbiology,2014,64(3):1405-1411.
[42]Elsayed O,Maillard E,Vuilleumier S,et al.Bacterial communities in batch and continuous-flow wetlands treating the herbicide S-metolachlor[J].Science of the Total Environment,2014,499:327-335.
[43]Zhang L,Gao G,Tang X,et al.Bacterial community changes along a salinity gradient in a Chinese wetland[J].Canadian Journal of Microbiology,2013,59(9):611-619.
[44]Baik K S,Park S C,Kim E M,et al.Diversity of bacterial community in freshwater of Woopo wetland[J].The Journal of Microbiology,2008,46(6):647-655.
[45]Buesing N,Filippini M,Bürgmann H,et al.Microbial communities in contrasting freshwater marsh microhabitats[J].FEMS Microbiology Ecology,2009,69(1):84-97.
[46]Wan T,Zhang G,Du F,et al.Combined biologic aerated filter and sulfur/ceramisite autotrophic denitrification for advanced wastewater nitrogen removal at low temperatures[J].Frontiers of Environmental Science&Engineering,2014,8(6):967-972.
[47]Huang X,Bai J,Li K R,et al.Characteristics of two novel cold-and salt-tolerant ammonia-oxidizing bacteria from Liaohe Estuarine Wetland[J].Marine Pollution Bulletin,2017,114(1):192-200.
[48]Li B,Chen H,Li N,et al.Spatio-temporal shifts in the archaeal community of a constructed wetland treating river water[J].Science of the Total Environment,2017,605:269-275.
[49]Long Y,Yi H,Chen S,et al.Influences of plant type on bacterial and archaeal communities in constructed wetland treating polluted river water[J].Environmental Science and Pollution Research,2016,23(19):19570-19579.
[50]Giovannoni S J,Stinql U.Molecular diversity and ecology of microbial plankton[J].Nature,2005,437(7057):343-348.
[51]Liu Y,Whitman W B.Metabolic,phylogenetic,and ecological diversity of the methanogenic archaea[J].Annals of the New York Academy of Sciences,2008,1125(1):171-189.
[52]张健,赵阳国,李海艳,等.黄海西北近岸沉积物中细菌群落空间分布特征[J].海洋学报,2010,32(2):118-127.Zhang J,Zhao Y G,Li H Y,et al.Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea[J].Acta Oceanologica Sinica,2010,32(2):118-127.