全真一气汤对慢性阻塞性肺疾病大鼠整联蛋白水平的影响
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摘要
目的:探讨全真一气汤对COPD大鼠ITGAM水平的影响。方法:建立COPD大鼠模型,随机分为中药组和模型组,中药组大鼠给予中药复方全真一气汤干预,肺组织中ITGAMmRNA及蛋白水平分别由实时荧光定量PCR、Western blot检测。结果:中药组大鼠ITGAMmRNA及蛋白表达水平均较模型组降低,其中两组大鼠ITGAMmRNA水平比较差异具有统计学意义(P<0.05)。结论:全真一气汤可能通过下调ITGAM表达影响肺血管炎症病变,从而延缓COPD病情进展。
Objective:To investigate the effect of Quanzhen Yiqi Decoction on ITGAM levels in COPD rats.Methods:The COPD rat model was established by smoking method,and the rats were divided into Chinese medicine group and model group by random number table method.The Chinese medicine group was treated with traditional Chinese medicine compound Quanzhen Yiqi Decoction.The model group was treated with physiological saline.After 60 days of intervention,the large group was taken.Right lung tissue was detected in rats,and the levels of ITGAM mRNA and protein in lung tissue were detected by real-time fluorescent quantitative PCR and Western blot.Results:The levels of protein expression and protein expression in the Chinese medicine group were lower than those in the model group.The difference of ITGAM mRNA between the two groups was statistically significant(P<0.05).Conclusion:Quanzhen Yiqi Decoction may delay the progression of COPD by reducing the ITGAM mRNA and protein levels in COPD rats,affecting pulmonary vascular inflammatory lesions.Therefore,we believe that Quanzhen Yiqi Decoction has a certain relationship with COPD pulmonary vascular remodeling.
Objective:To investigate the effect of Quanzhen Yiqi Decoction on ITGAM levels in COPD rats.Methods:The COPD rat model was established by smoking method,and the rats were divided into Chinese medicine group and model group by random number table method.The Chinese medicine group was treated with traditional Chinese medicine compound Quanzhen Yiqi Decoction.The model group was treated with physiological saline.After 60 days of intervention,the large group was taken.Right lung tissue was detected in rats,and the levels of ITGAM mRNA and protein in lung tissue were detected by real-time fluorescent quantitative PCR and Western blot.Results:The levels of protein expression and protein expression in the Chinese medicine group were lower than those in the model group.The difference of ITGAM mRNA between the two groups was statistically significant(P<0.05).Conclusion:Quanzhen Yiqi Decoction may delay the progression of COPD by reducing the ITGAM mRNA and protein levels in COPD rats,affecting pulmonary vascular inflammatory lesions.Therefore,we believe that Quanzhen Yiqi Decoction has a certain relationship with COPD pulmonary vascular remodeling.
引文
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[21]JAGARAPU J,KELCHTERMANS J,RONG M,et al.Leukadherin-1Attenuates Hyperoxia-induced Lung Injury in Neonatal Rats[J].American Journal of Respiratory Cell&Molecular Biology,2015,53(6).
[22]BERENDS C,DIJKHUIZEN B,DE MONCHY J G,et al.Inhibition of PAF-induced expression of CD11band shedding of L-selectin on human neutrophils and eosinophils by the type IV selective PDE inhibitor,rolipram[J].European Respiratory Journal,1997,10(5):1000-1007.
[23]王瑞幸,胡莹,穆云萍,等.人参皂甙Rb1对肺高压大鼠肺动脉血管环收缩效应的作用[C].台湾南部国际心脑血管科学研讨会,2013.
[24]郑洁珍,周大兴,顾静,等.怀牛膝有效成分对血管平滑肌收缩作用的影响[J].安徽中医药大学学报,2008,27(4):38-40.
[25]张团笑,牛彩琴,秦晓民.附子水煎剂对家兔离体肺动脉血管的舒张作用[J].中成药,2005,27(6):694-697.
[2]董蕾,从金鹏,张馨文,等.COPD肺动脉平滑肌细胞PPAR-γ表达、增殖/凋亡失衡与肺血管重塑关系的研究[J].国际呼吸杂志,2015,35(13):973-977.
[3]杨莉,李玉苹,蒲桂梅,等.FHL1与COPD大鼠肺血管重塑的关系及其机制[J].中华全科医学,2014,12(11):1727-1729.
[4]YAMAGATA T,SUGIURA H,YOKOYAMA T,et al.Overexpression of CD-11band CXCR1on circulating neutrophils:its possible role in COPD[J].Chest,2007,132(3):890-899.
[5]PI L,FU C,LU Y,et al.Vascular Endothelial Cell-Specific Connective Tissue Growth Factor(CTGF)Is Necessary for Development of Chronic Hypoxia-Induced Pulmonary Hypertension[J].Frontiers in Physiology,2018(9):138.
[6]冯兆张.冯氏锦囊秘录[M].北京:中国医药科技出版社,2011.
[7]李大治.全真一气汤治疗肾不纳气证慢性阻塞性肺疾病的基础和临床研究[D].福州:福建中医药大学,2014.
[8]王春娥,陈志斌,陈可强,等.全真一气汤对肾不纳气证慢性阻塞性肺疾病稳定期患者肺功能、运动耐量及MMP-9的影响[J].广西中医药,2017,40(1):14-16.
[9]王晓芳,陆慰萱.肺血管重塑中的炎症机制[J].国际呼吸杂志,2012,32(23):1816-1820.
[10]廖艺璇,陈亚红,米文君.慢性阻塞性肺疾病肺血管重塑的研究进展[J].生理科学进展,2016,47(4):295-299.
[11]JR C J,GLAWE J D,ALEXANDER J S,et al.VEGF164differentially regulates neutrophil and T cell adhesion through ItgaL-and ItgaM-dependent mechanisms[J].Am J Physiol Gastrointest Liver Physiol,2010,299(6):G1361-7.
[12]PENG C L,JIANG F,WANG B T,et al.[Interactions between susceptible genes and risk environmental factors in Chinese females with systemic lupus erythematosus[J].Chinese Journal of Preventive Medicine,2010,44(2):144.
[13]FAGERHOLM SC,VARIS M,STEFANIDAKIS M,et al.alpha-Chain phosphorylation of the human leukocyte CD11b/CD18(Mac-1)integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation[J].Blood,2006,108(10):3379.
[14]YANG W,ZHAO M,HIRANKARN N,etal.ITGAMisassociated with disease susceptibility and renal nephritis of systemic lupus erythematosus in Hong Kong Chinese and Thai-[J].Human Molecular Genetics,2009,18(11):2063-2070.
[15]KAPPELMAYER J,BERNABEI A,GIKAKIS N,et al.Upregulation of Mac-1 surface expression on neutrophils during simulated extracorporeal circulation[J].Journal of Laboratory&Clinical Medicine,1993,121(1):118.
[16]SCHOBER J M,CHEN N,GRZESZKIEWICZ T M,et al.Identification of integrin alpha(M)beta(2)as an adhesion receptor on peripheral blood monocytes for Cyr61(CCN1)and connective tissue growth factor(CCN2):immediateearly gene products expressed in atherosclerotic lesions[J].Blood,2002,99(12):4457-4465.
[17]FRID M G,BRUNETTI J A,BURKE D L,et al.Hypoxiainduced pulmonary vascular remodeling requires recruitment of circulating mesenchymal precursors of a monocyte/macrophage lineage[J].American Journal of Pathology,2006,168(2):659-669.
[18]FRID M G,LI M,GNANASEKHARAN M,et al.Sustained hypoxia leads to the emergence of cells with enhanced growth,migratory,and promitogenic potentials within the distal pulmonary artery wall[J].Am J Physiol Lung Cell Mol Physiol,2009,297(6):1059-1072.
[19]GAMBARYAN N,COHEN-KAMINSKY S,MONTANI D,et al.Circulating fibrocytes and pulmonary arterial hypertension[J].European Respiratory Journal,2012,39(1):210-212.
[20]AARON S D,ANGEL J B,LUNAU M,et al.Granulocyte inflammatory markers and airway infection during acute exacerbation of chronic obstructive pulmonary disease[J].A-merican Journal of Respiratory&Critical Care Medicine,2012,163(2):349.
[21]JAGARAPU J,KELCHTERMANS J,RONG M,et al.Leukadherin-1Attenuates Hyperoxia-induced Lung Injury in Neonatal Rats[J].American Journal of Respiratory Cell&Molecular Biology,2015,53(6).
[22]BERENDS C,DIJKHUIZEN B,DE MONCHY J G,et al.Inhibition of PAF-induced expression of CD11band shedding of L-selectin on human neutrophils and eosinophils by the type IV selective PDE inhibitor,rolipram[J].European Respiratory Journal,1997,10(5):1000-1007.
[23]王瑞幸,胡莹,穆云萍,等.人参皂甙Rb1对肺高压大鼠肺动脉血管环收缩效应的作用[C].台湾南部国际心脑血管科学研讨会,2013.
[24]郑洁珍,周大兴,顾静,等.怀牛膝有效成分对血管平滑肌收缩作用的影响[J].安徽中医药大学学报,2008,27(4):38-40.
[25]张团笑,牛彩琴,秦晓民.附子水煎剂对家兔离体肺动脉血管的舒张作用[J].中成药,2005,27(6):694-697.