3种杀虫剂亚致死浓度对斑翅果蝇和黑腹果蝇体内解毒酶的影响
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摘要
为探讨甲维盐、多杀菌素和高效氯氟氰菊酯亚致死剂量对斑翅果蝇和黑腹果蝇解毒酶的影响,本研究在3种杀虫剂亚致死剂量胁迫下,测定了两种果蝇体内乙酰胆碱酯酶(AchE)、谷胱甘肽转移酶(GSTs)、羧酸酯酶(CarE)和多功能氧化酶(MFO)的活性。结果表明,甲维盐可诱导斑翅果蝇体内(AchE)和(GSTs)的活性,而对黑腹果蝇则具有抑制作用;在乙基多杀菌素和高效氯氟氰菊酯亚致死剂量的处理下,两种果蝇体内CarE的活性升高,而黒腹果蝇体内的GSTs活性在剂量为LC20时会显著下降;另外,3种亚致死剂量的杀虫剂均可抑制斑翅果蝇的MFO的活性,而对黑腹果蝇的MFO活性具有明显的促进作用。由此可见,在亚致死剂量杀虫剂的处理下,两种果蝇体内的解毒酶活性均会发生变化,但应激反应并不相同,这为两种果蝇抗药性以及综合防治的差异分析提供了重要的指导作用。
To clarify the effects of emamectin, spinetoram and beta-cyfluthrinon with sublethal concentration on detoxification enzymes in Drosophila melanogaster and D. suzukii, the activities of detoxification enzymes were measured,such as acetylcholin esterase(AchE),glutathione transferase(GSTs),carboxylesterase(CarE) and multifunctional oxidase(MFO). The results showed that the activity of Ach E and GSTs in D. suzukii adult was activated by emamectin,while inhibited in D.melanogaster. The activity of Car E in two Drosophila speceis was activated by spinetoram and beta-cyfluthrinon. However,GSTs activity in D. melanogaster was significantly inhibited by spinetoram and beta-cyfluthrinon with the concentration of LC20. Moreover, these three pesticides with sublethal concentrations activated the activity of MFO in D. melanogaster,while inhibited that in D. suzukii. It was concluded that sublethal concentrations of pesticides could affect the activities of detoxification enzymes differently for D. melanogaster and D. suzukii,which will be guide for the research on the difference of pesticide-resistance and control strategy for D. melanogaster and D. Suzukii.
To clarify the effects of emamectin, spinetoram and beta-cyfluthrinon with sublethal concentration on detoxification enzymes in Drosophila melanogaster and D. suzukii, the activities of detoxification enzymes were measured,such as acetylcholin esterase(AchE),glutathione transferase(GSTs),carboxylesterase(CarE) and multifunctional oxidase(MFO). The results showed that the activity of Ach E and GSTs in D. suzukii adult was activated by emamectin,while inhibited in D.melanogaster. The activity of Car E in two Drosophila speceis was activated by spinetoram and beta-cyfluthrinon. However,GSTs activity in D. melanogaster was significantly inhibited by spinetoram and beta-cyfluthrinon with the concentration of LC20. Moreover, these three pesticides with sublethal concentrations activated the activity of MFO in D. melanogaster,while inhibited that in D. suzukii. It was concluded that sublethal concentrations of pesticides could affect the activities of detoxification enzymes differently for D. melanogaster and D. suzukii,which will be guide for the research on the difference of pesticide-resistance and control strategy for D. melanogaster and D. Suzukii.
引文
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Scott JG.Cytochromes P450 and insecticide resistance[J].InsectBiochemistry and Molecular Biology,1999,29(9):757-777.
Shen GM,Wang XN,Huang Y,et al.Tissue specific expression of genes encoding detoxification enzymes in the larvae of Bactrocera dorsalis underβ-Cypermethrin Stress[J].Scientia Agricultura Sinica,2015,48(19):3857-3865.[申光茂,王晓娜,黄勇,等.橘小实蝇幼虫解毒酶系基因应对高效氯氰菊酯胁迫的组织特异性表达[J].中国农业科学,2015,48(19):3857-3865]
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Wang GF,Zhang YJ,Bo LY,et al.Effects of spinosad on polyphenol oxidase and carboxylesterase in Spodoptera exigua[J].Chinese Journal of Pesticide Science,2003,5(2):40-46.[王光峰,张友军,柏连阳,等.多杀菌素对甜菜夜蛾多酚氧化酶和羧酸酯酶的影响[J].农药学学报,2003,5(2):40-46]
Wang SY,Yu Y,Liu YJ.Cross-resistance and biochemical resistance mechanisms of emamectin-benzoate resistant population of Frankliniella occidentalis[J].Journal of Plant Protection,2012,39(2):159-165.[王圣印,于毅,刘永杰.西花蓟马抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制[J].植物保护学报,2012,39(2):159-165]
Wang XQ.Impact of Sublethal Doses of Imidacloprid,Abamectin and Beta-cypermethrin on Two color Morphs of Pea Aphid[D].Gansu Agricultural University,2014.[王小强.三种杀虫剂对两种色型豌豆蚜亚致死效应研究[D].甘肃农业大学,2014]
Wu NJ,Chen WW,Wu SL,et al.Resistance to permethrin and activity of carboxylesterase in Aedes albopictus in Pingshan New District,Shenzhen City[J].Practical Preventive Medicine,2016,23(9):1123-1125.[吴能简,陈伟文,吴崧霖,等.深圳市坪山新区白纹伊蚊对氯菊酯的抗性及羧酸酯酶活性研究[J].实用预防医学,2016,23(9):1123-1125]
Zhang KC,Yan GH,Guo XJ,et al.Research review on spotted wing Drosophila[J].Journal of Fruit Science,2014,31(4):717-721.[张开春,闫国华,郭晓军,等.斑翅果蝇(Drosophila suzukii)研究现状[J].果树学报,2014,31(4):717-721]
Chaton PF,Ravanel P,Meyran JC,et al.The toxicological effects and bioaccumulation of fipronil in larvae of the mosquito Aedes aegypti in aqueous medium[J].Pesticide Biochemistry and Physiology,2001,69(3):183-188.
Cini A,Ioriatti C,Anfora G.A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management[J].Bulletin of Insectology,2012,65(1):149-160.
Dreves AJ,Walton VM,Fisher GC.A new pest attacking healthy ripening fruit in Oregon:Spotted wing Drosophila:Drosophila suzukii(Matsumura)[R].Corvallis,Or.:Extension Service,Oregon State University,2009.
Fang SM.Genomics of insect GSTs and its resistance[J].Newsletter of Sericultural Science,2011,4:28-35.[房守敏.昆虫谷胱甘肽-S-转移酶的基因组学研究及其介导的抗药性[J].蚕学通讯,2011,4:28-35]
Fournier D,Bride JM,Hoffmann F,et al.Acetylcholinesterase:Two types of modifications confer resistance to insecticide[J].Journal of Biological Chemistry,1992,267(20):14270-14274.
Guo LN,Li BL,Dong LM,et al.Occurrence and trapping methods of fruit fly in cherry orchard[J].Acta Agriculturae Borealioccidentalis Sinica,2014,23(2):55-60.[郭丽娜,李伯辽,董蓝蔓,等.樱桃果蝇发生与诱捕技术比较研究[J].西北农业学报,2014,23(2):55-60]
Guo JM.Bionomics of fruitflies,Drosophila melanogaster,damage cherry in Tianshui[J].Chinese Bulletin of Entomology,2007,44(5):31.[郭建明.樱桃新害虫黑腹果蝇的生物学特性[J].昆虫知识,2007,44(5):743-745]
Gong YH,Wu QJ,Zhang YJ,et al.Effect of sublethal concentrations of spinosad on the activities of detoxifying enzymes in the western flower thrips Frankliniella occidentalis[J].Chinese Journal of Pesticide Science,2009,11(4):427-433.[龚佑辉,吴青君,张友军,等.亚致死浓度多杀菌素对西花蓟马解毒酶系活力的影响[J].农药学学报,2009,11(4):427-433]
Jia BT,Hong SS,Zhang YC,et al.Effect of sublethal concentrations of abamectin on protective and detoxifying enzymes in Diagegma semclausum[J].Journal of Environmental Entomology,2016,38(5):990-995.[贾变桃,洪珊珊,张雨超,等.阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响[J].环境昆虫学报,2016,38(5):990-995]
Kong QM,Yu JB,Ruan SX,et al.Drosophila melanogaster and its control against sweet cherry fruit[J].Deciduous Fruits,2008,40(4):4-4.[孔庆敏,余建波,阮树兴,等.危害甜樱桃果实的黑腹果蝇及其防治[J].落叶果树,2008,40(4):4-4]
Kostaropoulos I,Papadopoulos A I,Metaxakis A,et al.Glutathione S-transferase in the defence against pyrethroids in insects[J].Insect Biochemistry and Molecular Biology,2001,31(4):313-319.
Leng XF,Tang ZH,Wang YZ.Molecular toxicology of insecticides and insect resistance[M].Beijing:China Agriculture Press,1996.[冷欣夫,唐振华,王荫长.杀虫药剂分子毒理学及昆虫抗药性.北京:中国农业出版社,1996]
Liao YZ,Yan SC,Cao CW.Effect of Methoxyfenozide on the activities of protective enzymes in larvaes of Lymantria dispar[J].Scientia Silvae Sinicae,2012,48(8):99-105.[廖月枝,严善春,曹传旺.甲氧虫酰肼对舞毒蛾幼虫解毒酶及其体内蛋白质表达的影响[J].林业科学,2012,48(8):99-105]
Lin QC,Zhai YF,Zhou XH,et al.Susceptibility of Drosophila suzukii to six common insecticides[J].Shandong Agricultural Sciences,2015,3:92-95.[林清彩,翟一凡,周仙红,等.铃木氏果蝇田间种群对6种常用杀虫剂敏感性测定[J].山东农业科学,2015,3:92-95]
Lin QC,Wang SY,Zhou CG,et al.Research progress in Drosophila suzukii[J].Acta Agriculturae Jiangxi,2013,25(10):75-78.[林清彩,王圣印,周成刚,等.铃木氏果蝇研究进展[J].江西农业学报,2013,10:75-78]
Liu K.Research progress on the occurrence and control of Drosophila[J].Northern Fruits,2014,3:1-3.[刘坤,甜樱桃果蝇发生与防治的研究进展[J].北方果树,2014,3:1-3]
Ren LM,Qin SN,Ding XT,et al.Research on the invasion and control of a fruit insect pest,Drosophila suzukii[J].Journal of Biosafety,2014,3:142-150.[任路明,秦胜楠,丁心婷,等.水果害虫铃木氏果蝇的入侵及其防控研究进展[J].生物安全学报,2014,3:142-150]
Ru Y,Chen YN,Shang SQ,et al.Effect of sublethal dose of avermectin on the activities of detoxifying enzymes in Tetranych usurticae[J].Journal of Gansu Agricultural University,2017,1:87-91.[汝阳,陈耀年,尚素琴,等.阿维菌素亚致死剂量对二斑叶螨解毒酶系的影响[J].甘肃农业大学学报,2017,1:87-91]
Scott JG.Cytochromes P450 and insecticide resistance[J].InsectBiochemistry and Molecular Biology,1999,29(9):757-777.
Shen GM,Wang XN,Huang Y,et al.Tissue specific expression of genes encoding detoxification enzymes in the larvae of Bactrocera dorsalis underβ-Cypermethrin Stress[J].Scientia Agricultura Sinica,2015,48(19):3857-3865.[申光茂,王晓娜,黄勇,等.橘小实蝇幼虫解毒酶系基因应对高效氯氰菊酯胁迫的组织特异性表达[J].中国农业科学,2015,48(19):3857-3865]
Shen JZ,Qian CF.Insect nultifunctional oxidase and its role in insecticide resistance[J].Pesticide Science and Administration,1993,1:12-15.[申继忠,钱传范.昆虫多功能氧化酶及其在杀虫剂抗性中的作用[J].农药科学与管理,1993,1:12-15]
Shi T,Che WN,Wu YD,et al.Metabolic mechanisms of resistance to emamectin and cypermethrin in field populations of Spodoptera exigua[J].Chinese Journal of Applied Entomology,2012,49(6):1482-1489.[侍甜,车午男,吴益东,等.甜菜夜蛾田间种群对甲氨基阿维菌素苯甲酸盐和高效氯氰菊酯的代谢抗性机制[J].应用昆虫学报,2012,49(6):1482-1489]
Tang ZH.Insect Resistance and its Control[M].Beijing:Agriculture Press,1993.[唐振华.昆虫抗药性及其治理[M].北京:农业出版社,1993]
Tao HY,Ma HM,Liu XQ,et al.Application of carboxylesterase in beta-cyfluthrin resistance surveillance of Musca domestica[J].Chinese Journal of Vector Biology and Control,2011,22(5):453-455,458.[陶卉英,马红梅,柳小青,等.羧酸酯酶用于家蝇高效氟氯氰菊酯抗性监测的研究[J].中国媒介生物学及控制杂志,2011,22(5):453-455,458]
Wang GF,Zhang YJ,Bo LY,et al.Effects of spinosad on polyphenol oxidase and carboxylesterase in Spodoptera exigua[J].Chinese Journal of Pesticide Science,2003,5(2):40-46.[王光峰,张友军,柏连阳,等.多杀菌素对甜菜夜蛾多酚氧化酶和羧酸酯酶的影响[J].农药学学报,2003,5(2):40-46]
Wang SY,Yu Y,Liu YJ.Cross-resistance and biochemical resistance mechanisms of emamectin-benzoate resistant population of Frankliniella occidentalis[J].Journal of Plant Protection,2012,39(2):159-165.[王圣印,于毅,刘永杰.西花蓟马抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制[J].植物保护学报,2012,39(2):159-165]
Wang XQ.Impact of Sublethal Doses of Imidacloprid,Abamectin and Beta-cypermethrin on Two color Morphs of Pea Aphid[D].Gansu Agricultural University,2014.[王小强.三种杀虫剂对两种色型豌豆蚜亚致死效应研究[D].甘肃农业大学,2014]
Wu NJ,Chen WW,Wu SL,et al.Resistance to permethrin and activity of carboxylesterase in Aedes albopictus in Pingshan New District,Shenzhen City[J].Practical Preventive Medicine,2016,23(9):1123-1125.[吴能简,陈伟文,吴崧霖,等.深圳市坪山新区白纹伊蚊对氯菊酯的抗性及羧酸酯酶活性研究[J].实用预防医学,2016,23(9):1123-1125]
Zhang KC,Yan GH,Guo XJ,et al.Research review on spotted wing Drosophila[J].Journal of Fruit Science,2014,31(4):717-721.[张开春,闫国华,郭晓军,等.斑翅果蝇(Drosophila suzukii)研究现状[J].果树学报,2014,31(4):717-721]