不同消毒剂对斑鳜受精卵孵化的影响
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摘要
采用药物浸泡的方法,在水温(23±1)℃、pH(6.8±0.5)、溶解氧(6.81±0.35)mg·L~(-1)条件下,研究不同质量浓度聚维酮碘、高锰酸钾和盐对斑鳜Siniperca scherzeri受精卵孵化效果的影响。结果表明:经过10min浸泡处理,随着聚维酮碘质量浓度增加,试验组孵化时间、孵化周期逐渐变短,显著大于对照组,30~50mg·L~(-1)聚维酮碘溶液处理组孵化率和畸形率与对照组没有显著差异,存活率显著高于对照组;高锰酸钾组随处理质量浓度升高,孵化时间、孵化周期逐渐缩短,但均高于对照组,孵化率呈现先升高后下降趋势,且各组均低于对照组,畸形率与对照组差异不显著,存活率显著高于对照组;盐水组中10、20mg·L~(-1)组孵化率显著高于40mg·L~(-1)组和对照组,孵化时间、孵化周期、畸形率和存活率各组和对照组之间差异不显著。因此,在斑鳜育苗过程中,建议使用质量浓度30~50mg·L~(-1)的10%聚维酮碘溶液或10~20mg·L~(-1)的盐水消毒受精卵10min以提高受精卵的孵化率;高锰酸钾具有较强的氧化性,建议生产上慎用。
Effect of povidone-iodine,potassium permanganate or saline in varied concentrations on hatching of fertilized eggs of Siniperca scherzeri was studied.The eggs were submerged in a water bath containing one of the 3 substances in different concentrations at(23±1)℃ and pH(6.8±0.5)with a dissolved oxygen of(6.81±0.35)mg·L~(-1).After soaking for 10 min,the egg-hatching rate and time were recorded for each treatment.The time and cycle in the povidone-iodine treatment group decreased gradually as the disinfectant concentration increased,and were significantly higher than those of control.Within the concentration of 30-50 mg·L~(-1),the survival rate of hatched eggs was significantly higher than,but not significantly different on the deformity rate and hatching rate from,those of control.In the treatments using potassium permanganate with an increasing concentration,the hatching time was gradually shortened but longer than control,while the lower-than-control hatching rate increased initially but followed by a downward trend.There was no significant difference in deformity rate,but a significantly higher survival rate,as compared to control.For the saline treatments,10 mg·L~(-1) and 20 mg·L~(-1) showed a significant higher hatching rate than either 40 mg·L~(-1) treatment or control.No significant differences were observed on the hatching time,survival rate and deformity rate between the treatments and control.Thus,in Scherzeri breeding it was recommended the application of either 10% povidone-iodine solution in the concentration of30-50 mg·L~(-1) or saline in 10-20 mg·L~(-1) to sanitize the eggs for 10 min be implemented to improve the hatchability.Since potassium permanganate is a strong oxidant,caution must be exercised in its application.
Effect of povidone-iodine,potassium permanganate or saline in varied concentrations on hatching of fertilized eggs of Siniperca scherzeri was studied.The eggs were submerged in a water bath containing one of the 3 substances in different concentrations at(23±1)℃ and pH(6.8±0.5)with a dissolved oxygen of(6.81±0.35)mg·L~(-1).After soaking for 10 min,the egg-hatching rate and time were recorded for each treatment.The time and cycle in the povidone-iodine treatment group decreased gradually as the disinfectant concentration increased,and were significantly higher than those of control.Within the concentration of 30-50 mg·L~(-1),the survival rate of hatched eggs was significantly higher than,but not significantly different on the deformity rate and hatching rate from,those of control.In the treatments using potassium permanganate with an increasing concentration,the hatching time was gradually shortened but longer than control,while the lower-than-control hatching rate increased initially but followed by a downward trend.There was no significant difference in deformity rate,but a significantly higher survival rate,as compared to control.For the saline treatments,10 mg·L~(-1) and 20 mg·L~(-1) showed a significant higher hatching rate than either 40 mg·L~(-1) treatment or control.No significant differences were observed on the hatching time,survival rate and deformity rate between the treatments and control.Thus,in Scherzeri breeding it was recommended the application of either 10% povidone-iodine solution in the concentration of30-50 mg·L~(-1) or saline in 10-20 mg·L~(-1) to sanitize the eggs for 10 min be implemented to improve the hatchability.Since potassium permanganate is a strong oxidant,caution must be exercised in its application.
引文
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[12]胡振禧,黄洪贵,吴妹英,等.温度对斑鳜胚胎发育的影响[J].淡水渔业,2014,44(3):104-107.
[13]吴建绍,林克冰,何丽斌,等.九种药物对斜带石斑鱼受精卵孵化率的影响[J].水产科学,2012,31(12):697-702.
[14]陈超然,孟长明,陈昌福.稳定性粉状二氧化氯对鱼类受精卵上附着菌的清除作用[J].华中农业大学学报,2001,20(6):568-570.
[15]张慧,谷伟,白庆利,等.双氧水预防白点鲑卵水霉病发生的效果研究[J].水产学杂志,2010,23(3):3-5.
[16]张铁奇,户国,谷伟,等.不同消毒剂对三种鲑科鱼受精卵成活率的影响[J].水产学杂志,2012,26(1):19-22.
[17]李天保,赵增元,王勇强,等.聚乙烯吡咯烷酮碘对病原体杀灭效果的研究[J].海水养殖,1997,52:12-16.
[18]杨先乐.新编渔药手册[M].北京:中国农业出版社,2005.
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[20]中华人民共和国农业部.NY5071-2002中华人民共和国农业行业标准:无公害食品渔用药物使用准则[S].北京:中国标准出版社,2002.
[21]李宝华,孙晓旺,杨建军,等.臭氧水对斑点叉尾鮰(Lctaluruspunctatus)受精卵孵化、幼苗培育的影响实验[J].天津水产,2000(1):15-17.
[22]孔祥迪,陈超,李炎璐,等.4种常用消毒药物对棕点石斑鱼(♀)鞍带石斑鱼(♂)受精卵孵化的影响[J].渔业科学进展,2014,35(5):122-127.
[23]朱庆红,李莉,赵昭.6种水产药物对金鱼苗的急性毒性试验[J].河南农业科学,2012,41(1):151-155.
[24]朱翠香.食盐水治苹果树腐烂效果好[J].河北农业科技,2002(12):20-21.
[25]李兴辉.观赏鱼季节性鱼病的防治[J].中国水产,2008(10):62-63.
[2]李思忠.鳜亚科鱼类地理分布的研究[J].动物学杂志,1991,26(4):40-44.
[3]梁旭方.国内外鳜类研究及养殖概况[J].水产科技情报,1996,23(1):13-17.
[4]杨受保.鳜类的资源利用及遗传多样性研究[J].水产科技情报,2003,30(3):121-125.
[5]冉辉,姚俊杰,杨兴,等.鳜亚科鱼类资源多样性的研究进展[J].贵州农业科学,2012,40(5):142-145.
[6]曾可为,王青云,高银爱,等.斑鳜的生物学及繁殖生物学的研究[J].内陆水产,2005,30(2):21-23.
[7]王青云,曾可为,夏儒龙,等.斑鳜的人工繁殖技术研究[J].内陆水产,2005,30(6):39-41.
[8]骆小年,李军,赵晓临,等.鸭绿江斑鳜规模化人工繁殖技术研究[J].水产学杂志,2010,23(2):25-28.
[9]宋庆波.辽宁本溪地区斑鳜繁殖技术[J].黑龙江水产,2011(2):9-10.
[10]黄洪贵,胡振禧,黄种持,等.斑鳜人工繁殖技术初报[J].福建水产,2014,36(2):154-158.
[11]胡振禧,黄洪贵,吴妹英,等.福建地区斑鳜胚胎与仔鱼早期发育的研究[J].上海海洋大学学报,2014,23(2):193-199.
[12]胡振禧,黄洪贵,吴妹英,等.温度对斑鳜胚胎发育的影响[J].淡水渔业,2014,44(3):104-107.
[13]吴建绍,林克冰,何丽斌,等.九种药物对斜带石斑鱼受精卵孵化率的影响[J].水产科学,2012,31(12):697-702.
[14]陈超然,孟长明,陈昌福.稳定性粉状二氧化氯对鱼类受精卵上附着菌的清除作用[J].华中农业大学学报,2001,20(6):568-570.
[15]张慧,谷伟,白庆利,等.双氧水预防白点鲑卵水霉病发生的效果研究[J].水产学杂志,2010,23(3):3-5.
[16]张铁奇,户国,谷伟,等.不同消毒剂对三种鲑科鱼受精卵成活率的影响[J].水产学杂志,2012,26(1):19-22.
[17]李天保,赵增元,王勇强,等.聚乙烯吡咯烷酮碘对病原体杀灭效果的研究[J].海水养殖,1997,52:12-16.
[18]杨先乐.新编渔药手册[M].北京:中国农业出版社,2005.
[19]光合.观赏鱼病害防治[J].渔业致富指南,2001(3):39.
[20]中华人民共和国农业部.NY5071-2002中华人民共和国农业行业标准:无公害食品渔用药物使用准则[S].北京:中国标准出版社,2002.
[21]李宝华,孙晓旺,杨建军,等.臭氧水对斑点叉尾鮰(Lctaluruspunctatus)受精卵孵化、幼苗培育的影响实验[J].天津水产,2000(1):15-17.
[22]孔祥迪,陈超,李炎璐,等.4种常用消毒药物对棕点石斑鱼(♀)鞍带石斑鱼(♂)受精卵孵化的影响[J].渔业科学进展,2014,35(5):122-127.
[23]朱庆红,李莉,赵昭.6种水产药物对金鱼苗的急性毒性试验[J].河南农业科学,2012,41(1):151-155.
[24]朱翠香.食盐水治苹果树腐烂效果好[J].河北农业科技,2002(12):20-21.
[25]李兴辉.观赏鱼季节性鱼病的防治[J].中国水产,2008(10):62-63.