酸性染料比色法测定燕麦中总生物碱含量条件优化
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摘要
采用酸性染料比色法优化燕麦中总生物碱含量的测定方法。以曲尼司特为标准品对照,检测波长为340 nm,对酸性染料种类、缓冲液种类和酸性染料用量进行优化,确定最佳检测条件。结果表明,最佳检测条件为:酸性染料为溴甲酚紫,缓冲液为pH5.4的磷酸二氢钠-氢氧化钠,酸性染料用量为3 m L。经测定,本方法线性范围为0.306～45.9 mg/L,回归方程为y=0.04837x+0.00822,R~2=0.9994,平均回收率为94.22%,RSD=1.79%。表明该方法具有良好的线性关系,精密度高,重复性好,且操作简单,可为燕麦总生物碱含量的检测提供参考与依据。
Optimization of the determination conditions of total Avenanthramides in oats by acid dye colorimetry.With the tranilast as a control standard at 340 nm,the acid dye species,buffer solution and acid dye dosage were optimized,and the best detection conditions were determined.The results showed that the best detection conditions were as follows:The acid dye was bromine cresol purple,the buffer was sodium hydrogen phosphate and sodium hydroxide with p H5.4,and the amount of acid dye was 3 m L.The linear range of this method was 0.306～45.9 mg/L,and the regression equation was y = 0.04837 x + 0.00822,R~2= 0.9994,and the average recovery rate was 94.22%,RSD = 1.79%.The results showed that the method had good linear relationship,high accuracy,good repeatability and simple operation,which would provide reference and basis for the determination of total avenanthramides content in oat.
Optimization of the determination conditions of total Avenanthramides in oats by acid dye colorimetry.With the tranilast as a control standard at 340 nm,the acid dye species,buffer solution and acid dye dosage were optimized,and the best detection conditions were determined.The results showed that the best detection conditions were as follows:The acid dye was bromine cresol purple,the buffer was sodium hydrogen phosphate and sodium hydroxide with p H5.4,and the amount of acid dye was 3 m L.The linear range of this method was 0.306～45.9 mg/L,and the regression equation was y = 0.04837 x + 0.00822,R~2= 0.9994,and the average recovery rate was 94.22%,RSD = 1.79%.The results showed that the method had good linear relationship,high accuracy,good repeatability and simple operation,which would provide reference and basis for the determination of total avenanthramides content in oat.
引文
[1]任祎,马挺军,崔林.燕麦生物碱及其潜在的保健作用[J].中国粮油学报,2012,27(1):132-136.
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[3]汪海波,谢笔钧,刘大川.燕麦中抗氧化成分的初步研究[J].食品科学,2003,24(7):62-66.
[4]魏红,张泽生,张民,等.裸燕麦麸皮提取物抗氧化活性研究[J].食品研究与开发,2007,28(2):14-17.
[5]Collins F W.Oat phenolics:avenanthramides,novel substituted N-cinnamoylanthranilate alkaloids from oat groats and hulls[J].Journal of Agricultural and Food Chemistry,1989,37(1):60-66.
[6]Peterson D M.Oat Antioxidants[J].Journal of Cereal Science,2001,33(2):115-129.
[7]Maliarova M,Mrazova V,Havrlentova M,et al.Optimization of parameters for extraction of avenanthramides from oat(Avena sativa L.)grain using response surface methodology(RSM)[J].Journal of the Brazilian Chemical Society,2015,26(11):2369-2378.
[8]Ishihara A,Kojima K,Fujita T,et al.New series of avenanthramides in oat seed[J].Bioscience Biotechnology and Biochemistry,2014,78(12):1975-1983.
[9]曾王旻.燕麦抗氧化活性物质提取及抗氧化活性研究[D].杨凌:西北农林科技大学,2011.
[10]任祎,马挺军,牛西午,等.燕麦生物碱提取物的抗氧化与降血脂作用研究[J].中国粮油学报,2008,23(6):103-106.
[11]Rasane P,Jha A,Sabikhi L,et al.Nutritional advantages of oats and opportunities for its processing as value added foods-a review[J].Journal of Food Science and Technology,2015,52(2):662-675.
[12]Hou J K,Abraham B,El Serag H.Dietary intake and risk of developing inflammatory bowel disease:A systematic review of the literature[J].American Journal of Gastroenterology,2011,106(4):563-573.
[13]Collins F W,Mullin W J.High-performance liquid chromatographic determination of avenanthramides,N-aroylanthranilic acid alkaloids from oats[J].Journal of Chromatography A,1988,445(2):363-370.
[14]Dimberg L H,Theander O,Lingnert H.Avenanthramides-a group of phenolic antioxidants in oats[J].Cereal Chemistry,1993,70:637-641.
[15]Emmons C L,Peterson D M.Antioxidant activity and phenolic contents of oat groats and hulls[J].Cereal Chemistry,1999,76(6):902-906.
[16]Jastrebova J,Skoglund M,Nilsson J,et al.Selective and sensitive LC-MS determination of avenanthramides in oats[J].Chromatographia,2006,63(9-10):419-423.
[17]Mattila P,Pihlava J M,Hellstrom J.Contents of phenolic acids,alkyl-and alkenylresorcinols,and avenanthramides in commercial grain products[J].Journal of Agricultural and Food Chemistry,2005,53(21):8290-8295.
[18]姜华,李军,徐强,等.白屈菜中总生物碱含量的酸性染料比色法测定[J].时珍国医国药,2017,28(4):854-856.
[19]曾王旻,马奕颜,李巨秀.超临界CO2流体萃取燕麦麸皮中的生物碱[J].农产品加工(学刊),2012(1):73-76.
[20]唐世蓉,张含庆.曼陀罗中总生物碱含量测定法的改进试验[J].中国药学杂志,1958,6(1):33-33.
[21]黄建明,郭济贤,孙明明,等.草乌中生物碱含量测定方法的研究[J].中药材,2002(12):878-880.
[22]朱礼艳.燕麦中活性成分的提取、分离与结构鉴定[D].无锡:江南大学,2012.
[23]毕重铭,张民.裸燕麦皂苷最佳提取条件的研究[J].环境与健康杂志,2011,28(10):920-921.
[24]李巨秀,李利霞,曾王旻,等.燕麦多酚化合物提取工艺及抗氧化活性的研究[J].中国食品学报,2010,10(5):14-21.
[25]谢佳颖,韩潆仪,任虹.燕麦生物碱研究进展[J].食品安全质量检测学报,2016,7(11):4536-4541.
[2]Bratt K,Sunnerheim K,Bryngelsson S,et al.Avenanthramides in oats(Avena sativa L.)and structure-antioxidant activity relationships[J].Journal of Agricultural and Food Chemistry,2003,51(3):594-600.
[3]汪海波,谢笔钧,刘大川.燕麦中抗氧化成分的初步研究[J].食品科学,2003,24(7):62-66.
[4]魏红,张泽生,张民,等.裸燕麦麸皮提取物抗氧化活性研究[J].食品研究与开发,2007,28(2):14-17.
[5]Collins F W.Oat phenolics:avenanthramides,novel substituted N-cinnamoylanthranilate alkaloids from oat groats and hulls[J].Journal of Agricultural and Food Chemistry,1989,37(1):60-66.
[6]Peterson D M.Oat Antioxidants[J].Journal of Cereal Science,2001,33(2):115-129.
[7]Maliarova M,Mrazova V,Havrlentova M,et al.Optimization of parameters for extraction of avenanthramides from oat(Avena sativa L.)grain using response surface methodology(RSM)[J].Journal of the Brazilian Chemical Society,2015,26(11):2369-2378.
[8]Ishihara A,Kojima K,Fujita T,et al.New series of avenanthramides in oat seed[J].Bioscience Biotechnology and Biochemistry,2014,78(12):1975-1983.
[9]曾王旻.燕麦抗氧化活性物质提取及抗氧化活性研究[D].杨凌:西北农林科技大学,2011.
[10]任祎,马挺军,牛西午,等.燕麦生物碱提取物的抗氧化与降血脂作用研究[J].中国粮油学报,2008,23(6):103-106.
[11]Rasane P,Jha A,Sabikhi L,et al.Nutritional advantages of oats and opportunities for its processing as value added foods-a review[J].Journal of Food Science and Technology,2015,52(2):662-675.
[12]Hou J K,Abraham B,El Serag H.Dietary intake and risk of developing inflammatory bowel disease:A systematic review of the literature[J].American Journal of Gastroenterology,2011,106(4):563-573.
[13]Collins F W,Mullin W J.High-performance liquid chromatographic determination of avenanthramides,N-aroylanthranilic acid alkaloids from oats[J].Journal of Chromatography A,1988,445(2):363-370.
[14]Dimberg L H,Theander O,Lingnert H.Avenanthramides-a group of phenolic antioxidants in oats[J].Cereal Chemistry,1993,70:637-641.
[15]Emmons C L,Peterson D M.Antioxidant activity and phenolic contents of oat groats and hulls[J].Cereal Chemistry,1999,76(6):902-906.
[16]Jastrebova J,Skoglund M,Nilsson J,et al.Selective and sensitive LC-MS determination of avenanthramides in oats[J].Chromatographia,2006,63(9-10):419-423.
[17]Mattila P,Pihlava J M,Hellstrom J.Contents of phenolic acids,alkyl-and alkenylresorcinols,and avenanthramides in commercial grain products[J].Journal of Agricultural and Food Chemistry,2005,53(21):8290-8295.
[18]姜华,李军,徐强,等.白屈菜中总生物碱含量的酸性染料比色法测定[J].时珍国医国药,2017,28(4):854-856.
[19]曾王旻,马奕颜,李巨秀.超临界CO2流体萃取燕麦麸皮中的生物碱[J].农产品加工(学刊),2012(1):73-76.
[20]唐世蓉,张含庆.曼陀罗中总生物碱含量测定法的改进试验[J].中国药学杂志,1958,6(1):33-33.
[21]黄建明,郭济贤,孙明明,等.草乌中生物碱含量测定方法的研究[J].中药材,2002(12):878-880.
[22]朱礼艳.燕麦中活性成分的提取、分离与结构鉴定[D].无锡:江南大学,2012.
[23]毕重铭,张民.裸燕麦皂苷最佳提取条件的研究[J].环境与健康杂志,2011,28(10):920-921.
[24]李巨秀,李利霞,曾王旻,等.燕麦多酚化合物提取工艺及抗氧化活性的研究[J].中国食品学报,2010,10(5):14-21.
[25]谢佳颖,韩潆仪,任虹.燕麦生物碱研究进展[J].食品安全质量检测学报,2016,7(11):4536-4541.