门氏护胃颗粒的薄层鉴别和含量测定研究
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摘要
目的:建立门氏护胃颗粒的薄层鉴别与含量测定研究方法。方法:采用薄层色谱对门氏护胃颗粒中人参、连翘、甘草、干姜进行定性鉴别,采用HPLC法对连翘酯苷A和连翘苷进行含量测定。结果:薄层色谱斑点分离效果良好,斑点显色清晰,且无干扰,重复性好。连翘酯苷A与连翘苷在0.61～6.1μg、0.246～2.46μg内线性关系良好(r=0.999);平均含量分别为1.00、0.37mg/g;平均回收率(n=6)分别为96.06%,97.09%。结论:主药薄层色谱定性鉴别特征性强,含量测定方法重现性好,操作简便,可作为门氏护胃颗粒的质量标准研究方法。
Objective: To establish a method for the TLC Identification and content determination of MENs' Huwei Granules. Methods: Thin layer chromatography was used to qualitatively identify MENs' Huwei Granules, and the contents of Forsythiaside A and Forsythin were determined by HPLC. Results: The results of TLC separation were good, the spots were clearly colored, no interference and good repeatability. Forsythoside A and Forsythin had good linearity(r=0.999) in the range of 0.61～6.1μg and 0.246～2.46μg, the average contents were 1.00 mg/g and 0.37 mg/g, respectively. The average recovery was96.06% and 97.09%, respectively. Conclusion: The main drug TLC qualitative identification of strong, good reproducibility of the determination of content, easy to operate, can be used as the quality standard MENs' Huwei Granules research methods.
Objective: To establish a method for the TLC Identification and content determination of MENs' Huwei Granules. Methods: Thin layer chromatography was used to qualitatively identify MENs' Huwei Granules, and the contents of Forsythiaside A and Forsythin were determined by HPLC. Results: The results of TLC separation were good, the spots were clearly colored, no interference and good repeatability. Forsythoside A and Forsythin had good linearity(r=0.999) in the range of 0.61～6.1μg and 0.246～2.46μg, the average contents were 1.00 mg/g and 0.37 mg/g, respectively. The average recovery was96.06% and 97.09%, respectively. Conclusion: The main drug TLC qualitative identification of strong, good reproducibility of the determination of content, easy to operate, can be used as the quality standard MENs' Huwei Granules research methods.
引文
[1]蒲香蓉,冯宇,王茂云,等.人参养荣汤在恶性肿瘤治疗中的运用及研究.吉林中医药,2017,37(5):505-508
[2]刘金钏,王晓利,张俊松,等.贯叶连翘在癌症治疗中应用前景的研究.中国现代中药,2006,8(2):22-24,38
[3]马磊,赵旭林,姬英,等.复方甘草酸苷在胃肠道肿瘤化疗中改善毒副作用的临床观察.实用肝脏病杂志,2008,11(1):38-39
[4]张晓芸,王兴华.炙甘草汤加减治疗化疗后胃肠道功能紊乱.山东中医杂志,2014,33(9):746
[5]丁爱华,华永庆,洪敏,等.海藻与甘草反药组合对大鼠离体回肠收缩及小鼠小肠推进功能的影响.中华中医药杂志,2014,29(1):87-90
[6]单晓梅,赖鹏华,吴狄,等.中医药对应激后胃肠损伤防治作用的实验研究现状.中华中医药杂志,2017,32(6):2602-2605
[7]国家药典委员会.中华人民共和国药典.4部.北京:中国医药科技出版社,2015
[8]马莉,唐健元,李祖伦,等.薄层扫描法测定胃康胶囊中人参皂苷Rg1和人参皂苷Rb1.中草药,2006,37(5):705-707
[9]曾祖平,何薇,穆树仁,等.中药复方制剂中连翘甙薄层层析鉴别.时珍国医国药,1999,10(11):833
[10]李敏,陈建蓉,李莉.兽用四逆汤中甘草、干姜的薄层色谱鉴别研究.宁夏农林科技,2010(5):38-39
[11]许佳,姜涛,陈廷贵,等.替代对照品法测定连翘中连翘酯苷A和连翘苷的含量.药物分析杂志,2013,33(10):1792-1795
[2]刘金钏,王晓利,张俊松,等.贯叶连翘在癌症治疗中应用前景的研究.中国现代中药,2006,8(2):22-24,38
[3]马磊,赵旭林,姬英,等.复方甘草酸苷在胃肠道肿瘤化疗中改善毒副作用的临床观察.实用肝脏病杂志,2008,11(1):38-39
[4]张晓芸,王兴华.炙甘草汤加减治疗化疗后胃肠道功能紊乱.山东中医杂志,2014,33(9):746
[5]丁爱华,华永庆,洪敏,等.海藻与甘草反药组合对大鼠离体回肠收缩及小鼠小肠推进功能的影响.中华中医药杂志,2014,29(1):87-90
[6]单晓梅,赖鹏华,吴狄,等.中医药对应激后胃肠损伤防治作用的实验研究现状.中华中医药杂志,2017,32(6):2602-2605
[7]国家药典委员会.中华人民共和国药典.4部.北京:中国医药科技出版社,2015
[8]马莉,唐健元,李祖伦,等.薄层扫描法测定胃康胶囊中人参皂苷Rg1和人参皂苷Rb1.中草药,2006,37(5):705-707
[9]曾祖平,何薇,穆树仁,等.中药复方制剂中连翘甙薄层层析鉴别.时珍国医国药,1999,10(11):833
[10]李敏,陈建蓉,李莉.兽用四逆汤中甘草、干姜的薄层色谱鉴别研究.宁夏农林科技,2010(5):38-39
[11]许佳,姜涛,陈廷贵,等.替代对照品法测定连翘中连翘酯苷A和连翘苷的含量.药物分析杂志,2013,33(10):1792-1795