仔猪断奶应激对肠道功能和组织结构影响的研究
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摘要
为了研究仔猪断奶应激对肠道功能和组织结构的影响,试验选择不同抗应激能力的莱芜猪和杜长大(DLY)商品仔猪各10头作为研究对象,分别于断奶前1周(21 d)、断奶当天(28 d)、断奶后1 d(29 d)、断奶后1周(35 d)、断奶后2周(42 d)5个阶段,测定十二指肠和空肠的消化酶活性、肠绒毛组织发育的变化。试验结果表明,两品种仔猪断奶后1周莱芜猪胰蛋白酶含量和活性比杜长大仔猪分别高81.48%(P<0.01)和21.44%(P<0.01);两品种仔猪的十二指肠肠绒毛长度在断奶后1周都比断奶前变短(P<0.05),断奶后1周莱芜猪的十二指肠和空肠肠绒毛长度比杜长大分别长73.62%(P<0.01)和65.63%(P<0.01)。莱芜猪比杜长大有更高的消化酶活性和肠道组织修复能力,具有更强的抗断奶应激的能力。
This experiment was performed to investigate the impact of weaning on Laiwu piglets and DLY piglets and assess their weaning anti-stress capabilities. A total of 20 piglets were used in this study. Digestive enzyme activity,intestinal villus histomorphology and microflora were also analyzed. The Laiwu piglets trypsin content and activity were 81. 48%( P < 0. 01) and 21. 44%( P < 0. 01) greater than those of DLY at 1 week post-weaning. The duodenal villus length of both breeds was shorter at 1 week postweaning than that during pre-weaning( P < 0. 05). The Laiwu piglets' duodenal and jejunum villus length were 73. 62%( P <0. 01) and 65. 63%( P < 0. 01) higher than DLY at 1 week post weaning. The Laiwu piglets have greater digestive enzyme activity,and intestinal repair ability than DLY to cope with weaning stress.
This experiment was performed to investigate the impact of weaning on Laiwu piglets and DLY piglets and assess their weaning anti-stress capabilities. A total of 20 piglets were used in this study. Digestive enzyme activity,intestinal villus histomorphology and microflora were also analyzed. The Laiwu piglets trypsin content and activity were 81. 48%( P < 0. 01) and 21. 44%( P < 0. 01) greater than those of DLY at 1 week post-weaning. The duodenal villus length of both breeds was shorter at 1 week postweaning than that during pre-weaning( P < 0. 05). The Laiwu piglets' duodenal and jejunum villus length were 73. 62%( P <0. 01) and 65. 63%( P < 0. 01) higher than DLY at 1 week post weaning. The Laiwu piglets have greater digestive enzyme activity,and intestinal repair ability than DLY to cope with weaning stress.
引文
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[4]de Passille A M,Pelletier G,Menard J,et al.Relationships of weight gain and behavior to digestive organ weight and enzyme activities in piglets[J].J Anim Sci,1989,67(11):2 921-2 929.
[5]PiéS,Lalles J,Blazy F,et al.Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets[J].J Nutrition,2004,134(3):641-647.
[6]Cabrera R A,Usry J L,Arrellano C,et al.Effects of creep feeding and supplemental glutamine or glutamine plus glutamate(Aminogut)on pre-and post-weaning growth performance and intestinal health of piglets[J].J Anim Sci Biotechnol,2013,4:29.
[7]Boudry G,Péron V,Hu3rou-Luron I L,et al.Weaning induces both transient and long-lasting modifications of absorptive,secretory,and barrier properties of piglet intestine[J].J Nutrition,2004,134(9):2 256-2 262.
[8]Pluske J R,Hampson D J,Williams I H.Factors influencing the structure and function of the small intestine in the weaned pig:a review[J].Livest Prod Sci,1997,51(1):215-236.
[9]Wang J,Chen L,Li P,et al.Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation[J].J Nutrition,2008,138(6):1 025-1 032.
[10]Castillo M,Martin-Orue S,Taylor-Pickard J,et al.Use of mannanoligosaccharides and zinc chelate as growth promoters and diarrhea preventative in weaning pigs:Effects on microbiota and gut function[J].J Anim Sci,2008,86(1):94-101.