湿热环境中温度和风速对妊娠母猪血清生化指标的影响
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摘要
【目的】本研究旨在研究湿热环境中不同温度和风速对妊娠母猪的相关生化指标的影响。【方法】本实验选取年龄、体重相近、身体健康的妊娠母猪共20头,饲养于水帘风机猪舍,分别测定不同风速区间、温度中猪只的皮质醇(Cortistatin,CORT),丙二醛(Malondialdehyde,MDA),热应激蛋白70(Heat Shock Protein 70,HSP70)水平。【结果】风速、温度以及二者的互作效应能显著影响妊娠母猪血清中CORT的浓度(P<0.05);血清中MDA和HSP70的浓度受到环境参数的显著影响,当温度超过等热区时,随着温度升高,血清中MDA和HSP70浓度显著升高(P<0.05),风速对这二者的影响不显著(P>0.05)。【结论】该实验结果显示,在我国西南地区,湿热环境会影响猪只体内的生化指标,造成一定的热应激,合理的增强圈舍风速,降低舍内温度能够一定程度的降低妊娠母猪的热应激。
【Objective】This study aimed to investigate the effects of different temperatures and wind speeds on biochemical indexes of pregnant sows in hot-humid environment. 【Method】In this experiment, 20 healthly pregnant sows with the similar body weight and age were selected; The CORT, MDA and HSP70 levels in pigs at different temperatures and wind speeds were measured. 【Result】The temperature, wind speed and their interactions significantly affected the concentration of CORT in the serum of pregnant sows(P<0.05); The concentration of MDA and HSP70 was significantly affected by environmental parameters, as the temperature increased, the concentration of MDA and HSP70 in serum increased significantly, the effect of wind speed on MDA and HSP70 was not significant. 【Conclusion】The results indicated that the hot-humid environment could affect the biochemical indexes in pigs and cause heat stress in the southwest of China, and the increase of wind speed and lower temperature could reduce the heat stress of pregnant sows.
【Objective】This study aimed to investigate the effects of different temperatures and wind speeds on biochemical indexes of pregnant sows in hot-humid environment. 【Method】In this experiment, 20 healthly pregnant sows with the similar body weight and age were selected; The CORT, MDA and HSP70 levels in pigs at different temperatures and wind speeds were measured. 【Result】The temperature, wind speed and their interactions significantly affected the concentration of CORT in the serum of pregnant sows(P<0.05); The concentration of MDA and HSP70 was significantly affected by environmental parameters, as the temperature increased, the concentration of MDA and HSP70 in serum increased significantly, the effect of wind speed on MDA and HSP70 was not significant. 【Conclusion】The results indicated that the hot-humid environment could affect the biochemical indexes in pigs and cause heat stress in the southwest of China, and the increase of wind speed and lower temperature could reduce the heat stress of pregnant sows.
引文
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[17]温昊. 营养水平对热应激 PIC 生长猪生产性能和生理生化指标的影响[D]. 重庆:西南大学,2009.
[18]Tang T, C. Wu, J. Li, et al. Stress-induced HSP70 from Musca domestica plays a functionally significant role in the immune system[J]. Journal of Insect Physiology, 2012, 58(9): 1226-1234.
[19]Romero R D, A. Montero Pardo, H.H. Montaldo, et al. Differences in body temperature, cell viability, and HSP-70 concentrations between Pelibuey and Suffolk sheep under heat stress[J]. Trop Anim Health Prod, 2013,45(8):1691-1696.
[20]李裕和. 热应激蛋白70 在热适应中的作用[J]. 中国运动医学杂志,2000,19(2): 182-183.
[21]Dang W, Y.h.Hu, M. Zhang, et al. Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus[J]. Fish & Shellfish Immunology, 2010, 29(4): 600-607.
[2]Hale B J, C.L. Hager, A.A. Al-Shaibi, et al. Autophagy induction in the pig follicular stage ovary during heat stress[J]. Journal of Animal Science, 2016, 94(supplement2): 63.
[3]Jing-Ping H U, L.P. Zheng, L.I. Ju-Feng, et al. Influence of Temperature on The Reproduction Performance of Breeding Pigs[J]. Acta Ecologiae Animalis Domastici, 2008(2).
[4]Ross J W, B.J. Hale, J.T. Seibert, et al. Physiological mechanisms through which heat stress compromises reproduction in pigs[J]. Molecular Reproduction & Development, 2017, 84(9):934-945.
[5]Borell E V, H. Dobson, A. Prunier. Stress, behaviour and reproductive performance in female cattle and pigs[J]. Hormones & Behavior, 2007, 52(1): 130.
[6]Hale B J, C.L. Hager, J.T. Seibert, et al. Heat Stress Induces Autophagy in Pig Ovaries during Follicular Development[J]. Biology of Reproduction, 2017, 97(3):426-437.
[7]Pollman D. Seasonal effects on sow herds: Industry experience and management strategies[J]. J. Anim. Sci, 2010, 88(Suppl 3): 9.
[8]张荣,庞晶,覃军. 近60年西南地区降水异常的气候特征分析[J]. 安徽农业科学,2012(30):14873-14875.
[9]蒲红州. 湿热环境对猪采食行为及生理生化指标影响的研究[J]. 雅安:四川农业大学,2014.
[10]刘胜军,卢庆萍,张宏福,等. 高温高湿环境对生长性能、血浆皮质醇浓度和免疫功能的影响[J]. 动物营养报,2010,22(5):1214-1219.
[11]Madej A, A.Mwanza, H. Kindahl, et al. Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F[J]. Acta Vet. Scand, 2005, 46(4): 249-256.
[12]张根军. 生长猪热应激反应模型及适宜监测指标筛选[D]. 北京:中国农业科学院硕士学位论文,2004.
[13]Rasooli A, M. Taha Jalali, M. Nouri, et al. Effects of chronic heat stress on testicular structures, serum testosterone and cortisol concentrations in developing lambs[J]. Anim Reprod Sci, 2010, 117(1-2): 55-59.
[14]王祖新,王之盛,王立志,等. 不同季节温湿度指数对奶牛生产性能和生理生化指标的影响[J]. 中国畜牧杂志,2009(23):60-63.
[15]Kinnula V L, J.D. Crapo, K.O. Raivio. Generation and disposal of reactive oxygen metabolites in the lung. Laboratory investigation[J]. Journal of Technical Methods and Pathology, 1995, 73(1): 3.
[16]刘胜军. 高温高湿环境对生长猪氧化还原状态和免疫功能的影响[D]. 北京:中国农业科学院,2010.
[17]温昊. 营养水平对热应激 PIC 生长猪生产性能和生理生化指标的影响[D]. 重庆:西南大学,2009.
[18]Tang T, C. Wu, J. Li, et al. Stress-induced HSP70 from Musca domestica plays a functionally significant role in the immune system[J]. Journal of Insect Physiology, 2012, 58(9): 1226-1234.
[19]Romero R D, A. Montero Pardo, H.H. Montaldo, et al. Differences in body temperature, cell viability, and HSP-70 concentrations between Pelibuey and Suffolk sheep under heat stress[J]. Trop Anim Health Prod, 2013,45(8):1691-1696.
[20]李裕和. 热应激蛋白70 在热适应中的作用[J]. 中国运动医学杂志,2000,19(2): 182-183.
[21]Dang W, Y.h.Hu, M. Zhang, et al. Identification and molecular analysis of a stress-inducible Hsp70 from Sciaenops ocellatus[J]. Fish & Shellfish Immunology, 2010, 29(4): 600-607.