饮水中洋葱伯克霍尔德菌的分离鉴定及消毒剂灭活试验
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摘要
依据GB/T 14926.41—2001《实验动物无菌动物生活环境及粪便样本的检测方法》从无菌环境中无菌操作取水样进行检测时,分离到一种粗短革兰氏阴性杆菌,进一步进行病原菌培养特性、生化鉴定和16S rRNA通用引物PCR扩增分析,结果为洋葱伯克霍尔德菌;并选用常用消毒剂对其进行悬液定量杀菌试验,84消毒液和75%乙醇对其作用10 min杀灭对数值≥5,50%甲酚皂溶液、新洁尔灭消毒液和10%癸甲溴铵溶液对其作用10 min杀灭对数值均<5。
According to the GB / T 14926. 41- 2001 " Experimental Animals Sterile Environment and Animal Fecal Sample Detection Method",the waters of sterile environment were taken aseptically and the pathogen was isolated to one kind of chunky gram-negative bacteria, and further carry out culture characteristics, biochemical identification and 16 S rRNA universal primers PCR amplification. The result showed that the pathogen was burkholderia cepacia. Common disinfectants were selected with inactivation test. The values of 84 disinfectant & ethanol 75% against the pathogen were larger than 5 for10 min,and the values of saponated cresol solution & bromogeramine & didecyl dimethyl ammonium bromide solution 10%against the pathogen were lower than 5 for 10 min.
According to the GB / T 14926. 41- 2001 " Experimental Animals Sterile Environment and Animal Fecal Sample Detection Method",the waters of sterile environment were taken aseptically and the pathogen was isolated to one kind of chunky gram-negative bacteria, and further carry out culture characteristics, biochemical identification and 16 S rRNA universal primers PCR amplification. The result showed that the pathogen was burkholderia cepacia. Common disinfectants were selected with inactivation test. The values of 84 disinfectant & ethanol 75% against the pathogen were larger than 5 for10 min,and the values of saponated cresol solution & bromogeramine & didecyl dimethyl ammonium bromide solution 10%against the pathogen were lower than 5 for 10 min.
引文
[1]李金钟.洋葱伯克霍尔德菌复合体的研究进展[J].临床检验杂志,2008,26(3):233-235.
[2]林丽蓉,姚世辉.洋葱伯克霍尔德菌研究进展[J].浙江检验医学,2003(4):6-7.
[3]贾彬,刘文山,杨江科,等.土壤中产脂肪酶洋葱伯克霍尔德菌的分离与鉴定[J].生物技术通报,2014,37(4):183-188.
[4]徐小平,李卓成,肖建,等.苍术消毒剂杀菌性能的实验观察[J].中华医院感染学杂志,2003,13(9):843-844.
[8]李皇,张耀平,梁林慧,等.洋葱伯克霍尔德菌在几种肠道选择性培养基上的生长比较及对V I T E K鉴定的影响[J].广西医科大学学报,2009,26(1):98-100.
[6]赵艳华,吕岳峰.洋葱伯克霍尔德菌研究进展[J].国际检验医学杂志,2006,27(7):651-652.
[7]吕琳,宋诗铎.洋葱伯克霍尔德复合体分型方法的研究进展[J].国外医药抗生素分册,2006,27(4):171-173.
[2]林丽蓉,姚世辉.洋葱伯克霍尔德菌研究进展[J].浙江检验医学,2003(4):6-7.
[3]贾彬,刘文山,杨江科,等.土壤中产脂肪酶洋葱伯克霍尔德菌的分离与鉴定[J].生物技术通报,2014,37(4):183-188.
[4]徐小平,李卓成,肖建,等.苍术消毒剂杀菌性能的实验观察[J].中华医院感染学杂志,2003,13(9):843-844.
[8]李皇,张耀平,梁林慧,等.洋葱伯克霍尔德菌在几种肠道选择性培养基上的生长比较及对V I T E K鉴定的影响[J].广西医科大学学报,2009,26(1):98-100.
[6]赵艳华,吕岳峰.洋葱伯克霍尔德菌研究进展[J].国际检验医学杂志,2006,27(7):651-652.
[7]吕琳,宋诗铎.洋葱伯克霍尔德复合体分型方法的研究进展[J].国外医药抗生素分册,2006,27(4):171-173.