朱顶红染色体核型分析及倍性鉴定
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摘要
以朱顶红新生根尖为材料,采用常规压片法进行染色体制片,对朱顶红属品种进行了染色体核型分析及倍性鉴定。结果表明:0.002 mol/L 8-羟基喹啉水溶液4℃预处理朱顶红根尖9—24 h,1 mol/L HCl溶液60℃解离根尖9 min获得的染色体制片效果最好。首次对朱顶红品种'柠檬冰糕'进行核型分析,发现其为三倍体朱顶红,核型公式为K(2n)=3x=33=12 m+12 sm+9 st,核型不对称系数(As.K)为70.19%,核型类型为3B型。根据染色体制片结果对6个朱顶红品种进行倍性鉴定表明:'凤蝶'为二倍体朱顶红,'特伦蒂诺'为三倍体朱顶红,'粉色惊奇''快车''苹果花''樱桃妮芙'均为四倍体朱顶红。
Taking the new root tips as materials, karyotype analysis and ploidy identification of Hippeastrum cultivars were carried out by traditional chromosome tabletting method. The results showed that the best chromosome morphology was observed when root tips treated with 0. 002 mol/L 8-Hydroxyquinoline for 9—24 h at 4 ℃ and 1 mol/L HC1 for 9 min at 60 ℃. The karyotype of Hippeastrum cultivar ' Lemon Sorbet ' was analyzed for the first time. It was found that ' Lemon Sorbet ' was triploid,the karyotype formula was K(2 n)=3 x=33 =12 m +12 sm +9 st,the asymmetrical karyotype coefficient( As. K) was 70. 19% and the karyotype type was "3 B". With the same method,the ploidy identification of 6 Hippeastrum cultivars showed that H. papilio was diploid,' Trentino ' was triploid,' Pink Surprise ',' Rapido ',' Apple Blossom ' and ' Cherry Nymph ' were all tetraploid.
Taking the new root tips as materials, karyotype analysis and ploidy identification of Hippeastrum cultivars were carried out by traditional chromosome tabletting method. The results showed that the best chromosome morphology was observed when root tips treated with 0. 002 mol/L 8-Hydroxyquinoline for 9—24 h at 4 ℃ and 1 mol/L HC1 for 9 min at 60 ℃. The karyotype of Hippeastrum cultivar ' Lemon Sorbet ' was analyzed for the first time. It was found that ' Lemon Sorbet ' was triploid,the karyotype formula was K(2 n)=3 x=33 =12 m +12 sm +9 st,the asymmetrical karyotype coefficient( As. K) was 70. 19% and the karyotype type was "3 B". With the same method,the ploidy identification of 6 Hippeastrum cultivars showed that H. papilio was diploid,' Trentino ' was triploid,' Pink Surprise ',' Rapido ',' Apple Blossom ' and ' Cherry Nymph ' were all tetraploid.
引文
[1]HANNEKE V D,MINEKE K.The complete encyclopedia of bulbs&tubes[M].Lisse:Rebo publishers,2001:159-160.
[2]LCZUKI A,WINKELMANN T,RICHARTZ S,et al.In vitro propagation of Hippeastrum×chmielii Chm.-influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems[J].Plant Cell Tissue&Organ Culture,2005,83(3):339-346.
[3]陈俊愉,程绪珂.花经[M].上海:上海文化出版社,1990:604-605.
[4]张林,成海钟,周玉珍,等.朱顶红的研究进展[J].江苏农业科学,2011,39(5):225-228.
[5]马慧,王琪,袁燕波,等.朱顶红属植物种质资源及园林应用[J].世界林业研究,2012,25(4):28-33.
[6]王中轩,魏迟,廉玉芹,等.中国原产4种野生百合的核型分析[J].园艺学报,2013,40(11):2207-2212.
[7]王春彦,罗凤霞,李玉萍,等.4个洋水仙品种的核型分析[J].西北植物学报,2011,31(8):1577-1581.
[8]尹翠翠,张燕,张景华,等.秋水仙素诱导杂交兰四倍体及倍性鉴定[J].核农学报,2010,24(3):518-521.
[9]管苇,张云霞,杨树琼,等.黄瓜倍性材料创制及染色体组成的FISH鉴定[J].中国农业科学,2014,47(17):3513-3522.
[10]杨宁,谈永霞,李巧峡,等.百里香染色体制片优化及核型分析[J].草业学报,2012,21(1):184-189.
[11]杜胜利,韩毅科,魏爱民,等.黄瓜倍性鉴定方法的研究[J].园艺学报,2002,29(3):280-281.
[12]邹琦丽,秦志祥.朱顶红染色体数目和核型[J].广西植物,1994(1):37-38.
[13]陈瑞娇.白肋朱顶红染色体核型分析[J].北方园艺,2013(8):110-111.
[14]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302.
[15]LEVAN A,FREDGA K,SANDBERG A A.Nomenclature for centromeric position on chromosomes[J].Hereditas,1964,52(2):201-220.
[16]ARANO H.Cytological Studies in Subfamily Carduoideae(Compositae)of Japan[J].Bot Mag,1963,76:32-39.
[17]STEBBINS G L.Chromosome evolution in higher plants[M].London:Edward Arnold,1971:87-123.
[18]赵晨宇.蓝花丹染色体核型分析研究[D].雅安:四川农业大学,2013.
[19]周翼虎.山药的染色体制片技术优化与核型分析[D].呼和浩特:内蒙古农业大学,2014.
[20]曾佳诗,王东旭,吴阳清,等.勋章菊品种核型分析[J].园艺学报,2015,42(12):2512-2518.
[2]LCZUKI A,WINKELMANN T,RICHARTZ S,et al.In vitro propagation of Hippeastrum×chmielii Chm.-influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems[J].Plant Cell Tissue&Organ Culture,2005,83(3):339-346.
[3]陈俊愉,程绪珂.花经[M].上海:上海文化出版社,1990:604-605.
[4]张林,成海钟,周玉珍,等.朱顶红的研究进展[J].江苏农业科学,2011,39(5):225-228.
[5]马慧,王琪,袁燕波,等.朱顶红属植物种质资源及园林应用[J].世界林业研究,2012,25(4):28-33.
[6]王中轩,魏迟,廉玉芹,等.中国原产4种野生百合的核型分析[J].园艺学报,2013,40(11):2207-2212.
[7]王春彦,罗凤霞,李玉萍,等.4个洋水仙品种的核型分析[J].西北植物学报,2011,31(8):1577-1581.
[8]尹翠翠,张燕,张景华,等.秋水仙素诱导杂交兰四倍体及倍性鉴定[J].核农学报,2010,24(3):518-521.
[9]管苇,张云霞,杨树琼,等.黄瓜倍性材料创制及染色体组成的FISH鉴定[J].中国农业科学,2014,47(17):3513-3522.
[10]杨宁,谈永霞,李巧峡,等.百里香染色体制片优化及核型分析[J].草业学报,2012,21(1):184-189.
[11]杜胜利,韩毅科,魏爱民,等.黄瓜倍性鉴定方法的研究[J].园艺学报,2002,29(3):280-281.
[12]邹琦丽,秦志祥.朱顶红染色体数目和核型[J].广西植物,1994(1):37-38.
[13]陈瑞娇.白肋朱顶红染色体核型分析[J].北方园艺,2013(8):110-111.
[14]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302.
[15]LEVAN A,FREDGA K,SANDBERG A A.Nomenclature for centromeric position on chromosomes[J].Hereditas,1964,52(2):201-220.
[16]ARANO H.Cytological Studies in Subfamily Carduoideae(Compositae)of Japan[J].Bot Mag,1963,76:32-39.
[17]STEBBINS G L.Chromosome evolution in higher plants[M].London:Edward Arnold,1971:87-123.
[18]赵晨宇.蓝花丹染色体核型分析研究[D].雅安:四川农业大学,2013.
[19]周翼虎.山药的染色体制片技术优化与核型分析[D].呼和浩特:内蒙古农业大学,2014.
[20]曾佳诗,王东旭,吴阳清,等.勋章菊品种核型分析[J].园艺学报,2015,42(12):2512-2518.