氰戊菊酯对雄性大鼠激素昼夜节律性的影响
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摘要
目的研究氰戊菊酯对雄性大鼠激素昼夜节律性的影响。方法选择健康雄性SD大鼠12只,分为处理组(氰戊菊酯60 mg/kg·bw)和对照组(食用油)各6只,持续灌胃染毒30 d后,分别在不同昼夜时间点(Circadian Time, CT)CT9∶00、CT13∶00、CT17∶00、CT21∶00、CT1∶00和CT5∶00对大鼠进行眼眶静脉取血,随后检测血清褪黑素(MLT)、睾酮(T)、雌激素(E2)、卵泡生成激素(FSH)、促黄体生成素(LH)和促性腺激素释放激素(GnRH)的水平。结果氰戊菊酯作用后,大鼠血清中MLT水平在CT9∶00、CT17∶00、CT21∶00、CT1∶00和CT5∶00表达降低,分别下降了8.51%、16.00%、18.69%、16.81%和13.97%;T水平在CT9∶00、CT13∶00、CT17∶00、CT21∶00和CT1∶00表达降低,分别下降了19.79%、23.96%、18.80%、15.31%和13.20%;E2水平在各时间点均明显升高,分别升高了59.34%、39.17%、52.73%、68.76%、32.80%和31.97%;FSH在各时间点分别升高了34.50%、24.44%、107.07%、38.40%、37.33%和27.86%;LH水平在CT9:00、CT21:00和CT1:00表达明显升高,分别升高了27.55%、28.43%和47.63%;GnRH水平在CT9∶00、CT13∶00、CT21∶00和CT1∶00表达明显升高,分别升高了40.92%、19.64%、19.12%和22.75%。MLT、T、LH、GnRH在血清中含量呈现24 h的昼夜节律性,峰值时间分别为23∶52、16∶58、17∶16和19∶57;氰戊菊酯暴露后,大鼠血清中MLT、TLH和GnRH的昼夜节律性消失。结论氰戊菊酯不仅可以影响雄性大鼠血清中激素的水平,而且能干扰激素的昼夜节律性。
Objective To study the effect of fenvalerate on the circadian rhythm of hormones in male rats. Methods Twelve healthy male Sprague-Dawley rats were divided into two groups: treatment group(Fenvalerate 60 mg/kg·bw) and control group(edible oil), respectively. After continuous gavage for 30 days, Rats were subjected to blood sampling from the orbital vein at different time points(CT9∶ 00, CT13∶00, CT17∶00, CT21∶00, CT1∶00, CT5∶00), then detect the levels of serum melatonin(MLT), testosterone(T), and estrogen(E2), follicle stimulating hormone(FSH), luteinizing hormone(LH), and gonadotropin releasing hormone(GnRH). Result After the action of fenvalerate, the levels of melatonin in the serum of rats decreased at CT9∶00, CT17∶00, CT21∶00, CT1∶00 and CT5∶00, by 8.51%, 16.00%, 18.69%, 16.81%, 13.97% respectively; testosterone levels decreased at CT9∶00, CT13∶00, CT17∶00, CT21∶00, CT1∶00, by 19.79%, 23.96%, 18.80%, 15.31%, 13.20%, respectively; The E2 level increased significantly at each time point, by 59.34%, 39.17%, 52.73%, 68.76%, 32.80%, and 31.97%, respectively. FSH increased by 34.50%, 24.44%, 107.07%. 38.40%, 37.33%, 27.86% at each time point; LH levels were significantly increased at CT9∶00, CT21∶00, CT1∶00, by 27.55%, 28.43%, and 47.63%, respectively; GnRH levels were significantly increased at CT9∶00, CT13∶00, CT21∶00,CT1∶00, by 40.92%, 19.64%, 19.12% and 22.75%, respectively. MLT, T, LH, and GnRH showed a 24-hour circadian rhythm in serum, with peak times of 23∶52, 16∶58, 17∶16, and 19∶57, respectively. After exposure to fenvalerate, the serum in rats was blackened. The circadian rhythm of MLT, T, LH, and GnRH disappeared. Conclusion Fenvalerate can not only affect the serum levels of hormones in male rats, but also interfere with the circadian rhythm of hormones.
Objective To study the effect of fenvalerate on the circadian rhythm of hormones in male rats. Methods Twelve healthy male Sprague-Dawley rats were divided into two groups: treatment group(Fenvalerate 60 mg/kg·bw) and control group(edible oil), respectively. After continuous gavage for 30 days, Rats were subjected to blood sampling from the orbital vein at different time points(CT9∶ 00, CT13∶00, CT17∶00, CT21∶00, CT1∶00, CT5∶00), then detect the levels of serum melatonin(MLT), testosterone(T), and estrogen(E2), follicle stimulating hormone(FSH), luteinizing hormone(LH), and gonadotropin releasing hormone(GnRH). Result After the action of fenvalerate, the levels of melatonin in the serum of rats decreased at CT9∶00, CT17∶00, CT21∶00, CT1∶00 and CT5∶00, by 8.51%, 16.00%, 18.69%, 16.81%, 13.97% respectively; testosterone levels decreased at CT9∶00, CT13∶00, CT17∶00, CT21∶00, CT1∶00, by 19.79%, 23.96%, 18.80%, 15.31%, 13.20%, respectively; The E2 level increased significantly at each time point, by 59.34%, 39.17%, 52.73%, 68.76%, 32.80%, and 31.97%, respectively. FSH increased by 34.50%, 24.44%, 107.07%. 38.40%, 37.33%, 27.86% at each time point; LH levels were significantly increased at CT9∶00, CT21∶00, CT1∶00, by 27.55%, 28.43%, and 47.63%, respectively; GnRH levels were significantly increased at CT9∶00, CT13∶00, CT21∶00,CT1∶00, by 40.92%, 19.64%, 19.12% and 22.75%, respectively. MLT, T, LH, and GnRH showed a 24-hour circadian rhythm in serum, with peak times of 23∶52, 16∶58, 17∶16, and 19∶57, respectively. After exposure to fenvalerate, the serum in rats was blackened. The circadian rhythm of MLT, T, LH, and GnRH disappeared. Conclusion Fenvalerate can not only affect the serum levels of hormones in male rats, but also interfere with the circadian rhythm of hormones.
引文
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[11] Reiter RJ,Tamura H,Tan DX et al.Melatonin and the circadian system:contributions to successful female reproduction[J].Fertil Steril,2014,102(2):321-328.
[12] Haldar C,Sharma S,Singh SS.Reproductive phase dependent circadian variations of plasma melatonin,testosterone,thyroxine and corticosterone in Indian palm squirrel[J].Biol Rhythm Res,2006,37(1):1-10.
[13] 秦粉菊,袁红霞,陈丽莉,等.氰戊菊酯对雄性大鼠生殖系统的时间毒性[J].卫生研究,2012,41(6):905-910.
[14] Rhee JS,Kim BM,Lee BY,et al.Cloning of circadian rhythmic pathway genes and perturbation of oscillation patterns in endocrine disrupting chemicals (EDCs)-exposed mangrove killifish Kryptolebias marmoratus[J].Comp Biochem Physiol C Toxicol Pharmacol,2014,164:11-20.
[2] 姚克文,王介东.氰戊菊酯对大鼠精子及生殖激素的影响[J].生殖医学杂志,2008,17(1):58-61.
[3] Perry MJ,Venners SA,Barr DB,et al.Environmental pyrethroid and organophosphorus insecticide exposures and sperm concentration[J].Reprod Toxicol,2007,23(1):113-118.
[4] 谈立峰,王守林,孙雪照,等.职业性接触氰戊菊酯农药对精液质量的影响[J].中华男科学杂志,2002,8(4):273-276.
[5] Man GCW,Tao Z,Chen X,et al.The regulations and role of circadian clock and melatonin in uterine receptivity and pregnancy—An immunological perspective[J].Am J of Reprod Immunol,2017,78(2).doi:10.1101/aji.12715.
[6] Gooren LJ,Harmsenlouman W,Van KH,et al.Basal and LHRH-stimulated gonadotropin levels and the circadian rhythm of testosterone and the effect of exogenous testosterone thereon[J].Andrologia,1984,16(3):249-255.
[7] Singh SS,Haldar C.Biological significance of daily variation in immunity of Perdicula asiatica:role of melatonin and testosterone[J].Biol Rhythm Res,2007,38(2):95-106.
[8] Guo Y,Shen O,Han J,et al.Circadian rhythm genes mediate fenvalerate-induced inhibition of testosterone synthesis in mouse Leydig cells[J].J Toxicol Environ Health A,2017,80(23/24):1314-1320.
[9] Zhang J,Hu Y,Guo J,et al.Fenvalerate decreases semen quality in puberty rat through germ cell apoptosis[J].Andrologia,2018,50(9):e13079.
[10] 胡静熠,王守林,赵人琤,等.氰戊菊酯对雄性大鼠生殖内分泌系统的影响[J].中华男科学,2002,8(1):18-21.
[11] Reiter RJ,Tamura H,Tan DX et al.Melatonin and the circadian system:contributions to successful female reproduction[J].Fertil Steril,2014,102(2):321-328.
[12] Haldar C,Sharma S,Singh SS.Reproductive phase dependent circadian variations of plasma melatonin,testosterone,thyroxine and corticosterone in Indian palm squirrel[J].Biol Rhythm Res,2006,37(1):1-10.
[13] 秦粉菊,袁红霞,陈丽莉,等.氰戊菊酯对雄性大鼠生殖系统的时间毒性[J].卫生研究,2012,41(6):905-910.
[14] Rhee JS,Kim BM,Lee BY,et al.Cloning of circadian rhythmic pathway genes and perturbation of oscillation patterns in endocrine disrupting chemicals (EDCs)-exposed mangrove killifish Kryptolebias marmoratus[J].Comp Biochem Physiol C Toxicol Pharmacol,2014,164:11-20.