基于SSR分子标记的萝卜遗传多样性研究
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摘要
为促进萝卜种质资源保存以及萝卜品种改良,分别以白萝卜品种734、理想大根、大将军、黄州萝卜和红萝卜品种红宝、红园、七叶红、262和一尺红为试验材料,利用SSR对上述萝卜地方品种以及常规品种开展遗传多样性研究。首先对上述萝卜材料的田间性状(叶型、皮色)进行了观察,进而利用筛选得到的9对SSR(微卫星DNA,简单重复序列)引物对上述萝卜样品进行检测。结合电泳条带和NTSYS软件对九种萝卜的遗传多样性进行分析。结果显示9个品种之间被分为两大类群,类群一包括除七叶红之外的8个品种,且每个品种在不同的相似系数间表现出了多样性,类群二则仅包括七叶红。第一个类群内又有两个明显的分支,其中一个分支包括理想大根、724、大将军、黄州萝卜4种,其皮色表现为白色;另一个分支包括一尺红、红宝、262和红园4种,其皮色表现为红色,与田间观察的情况一致。
In order to promote the preservation and breed improvement of radish germplasm resources,nine varieties of radish were used as the experimental materials,which tried to study on genetic diversity the local and conventional radish varieties mentioned above using SSR.Firstly,field characters(leaf shape and skin color)of the above radish materials were observed,then,9 pairs of SSR primers were screened to detect the above radish samples.The genetic diversity of nine radish species was analyzed by electrophoretic banding and NTSYS software.The results showed that the nine varieties of radish were divided into two groups,group 1 includes 8 varieties except Qiyehong radish,and each variety showed diversity among different similarity coefficients,group 2 includes only Qiyehong radish.There were two distinct branches in the first group,one branch includes four radishes,Lixiangdagen radish,734 radish,General radish,and Huangzhou radish,whose skin color is white;another branch includes four radishes,the Yichihong radish,Hongbao radish,262 radish,and Red garden radish,whose skin color is red,which was consistent with field observations.
In order to promote the preservation and breed improvement of radish germplasm resources,nine varieties of radish were used as the experimental materials,which tried to study on genetic diversity the local and conventional radish varieties mentioned above using SSR.Firstly,field characters(leaf shape and skin color)of the above radish materials were observed,then,9 pairs of SSR primers were screened to detect the above radish samples.The genetic diversity of nine radish species was analyzed by electrophoretic banding and NTSYS software.The results showed that the nine varieties of radish were divided into two groups,group 1 includes 8 varieties except Qiyehong radish,and each variety showed diversity among different similarity coefficients,group 2 includes only Qiyehong radish.There were two distinct branches in the first group,one branch includes four radishes,Lixiangdagen radish,734 radish,General radish,and Huangzhou radish,whose skin color is white;another branch includes four radishes,the Yichihong radish,Hongbao radish,262 radish,and Red garden radish,whose skin color is red,which was consistent with field observations.
引文
[1]郎丰庆.萝卜的食疗和药用[J].山东蔬菜,1997(2):57-58.
[2]王春丽,张雪清,张贵生.萝卜的研究价值及开发应用前景[J].长江蔬菜,2011(10):11-14.
[3]贾豪,魏小春,姚秋菊,等.辣椒SSR标记种质资源遗传多样性的分析[J].种质资源,2017,15(1):353-363.
[4]李鸿雁,米福贵,李志勇,等.扁蓿豆遗传多样性研究初探[J].草业与畜牧,2007(1):20-23.
[5]石莹,李穆,何平,等.SNP技术及其在作物研究上的应用[J].农业科学,2014,4(3):60-65.
[6]尹承苗,王功帅,李园园,等.连作苹果园土壤真菌的T-RFLP分析[J].生态学报,2014(4):837-846.
[7]邱洋,李锡香,等.利用SSR标记构建萝卜种质资源分子身份证[J].植物遗传资源学报,2014,15(3):648-654.
[8]Sunar S,Yildirim N,Sengul M,et al.Genetic diversity and relationships detected by ISSR and RAPD analysis among Aethionema species growing in Eastern Anatolia(Turkey)[J].Comptesrendus-Biologies,2016,339(34):147-151.
[9]王庆彪,张扬勇,庄木,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J].中国农业科学,2014(1):111-121.
[10]El-Esawi M A,Germaine K,Bourke P,et al.Genetic diversity and populationstructure of Brassica oleracea germplasm inIreland using SSR markers[J].ComptesrendusBiologies,2016,339:133-140.
[11]方平,陈发波,姚启伦,等.肉质色不同萝卜遗传多样性的SSR分子标记分析[J].植物遗传资源学报,2012,13(2):226-232.
[12]张颖.应用DNA分子标记鉴定几种蔬菜作物种子纯度和真实性的探究[D].杭州:浙江大学,2008.
[13]徐建波,张晓康,李德超,等.夏秋萝卜品种比较试验[J].上海蔬菜,2015(3):19-20.
[14]李小乔.低温处理对萝卜开花习性影响规律研究[J].长江蔬菜,2014(8):33-35.
[2]王春丽,张雪清,张贵生.萝卜的研究价值及开发应用前景[J].长江蔬菜,2011(10):11-14.
[3]贾豪,魏小春,姚秋菊,等.辣椒SSR标记种质资源遗传多样性的分析[J].种质资源,2017,15(1):353-363.
[4]李鸿雁,米福贵,李志勇,等.扁蓿豆遗传多样性研究初探[J].草业与畜牧,2007(1):20-23.
[5]石莹,李穆,何平,等.SNP技术及其在作物研究上的应用[J].农业科学,2014,4(3):60-65.
[6]尹承苗,王功帅,李园园,等.连作苹果园土壤真菌的T-RFLP分析[J].生态学报,2014(4):837-846.
[7]邱洋,李锡香,等.利用SSR标记构建萝卜种质资源分子身份证[J].植物遗传资源学报,2014,15(3):648-654.
[8]Sunar S,Yildirim N,Sengul M,et al.Genetic diversity and relationships detected by ISSR and RAPD analysis among Aethionema species growing in Eastern Anatolia(Turkey)[J].Comptesrendus-Biologies,2016,339(34):147-151.
[9]王庆彪,张扬勇,庄木,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J].中国农业科学,2014(1):111-121.
[10]El-Esawi M A,Germaine K,Bourke P,et al.Genetic diversity and populationstructure of Brassica oleracea germplasm inIreland using SSR markers[J].ComptesrendusBiologies,2016,339:133-140.
[11]方平,陈发波,姚启伦,等.肉质色不同萝卜遗传多样性的SSR分子标记分析[J].植物遗传资源学报,2012,13(2):226-232.
[12]张颖.应用DNA分子标记鉴定几种蔬菜作物种子纯度和真实性的探究[D].杭州:浙江大学,2008.
[13]徐建波,张晓康,李德超,等.夏秋萝卜品种比较试验[J].上海蔬菜,2015(3):19-20.
[14]李小乔.低温处理对萝卜开花习性影响规律研究[J].长江蔬菜,2014(8):33-35.