浙南小麦核心育种亲本高分子量谷蛋白亚基组成分析
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摘要
为了解浙南小麦核心育种亲本的品质遗传基础,采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术对28份核心品种(系)的高分子量谷蛋白亚基(HMW-GS)组成进行分析。结果表明:供试品种(系)在Glu-A1位点具有Null(57. 14%)和1(42. 86%) 2种类型;在Glu-B1位点具有7+8(67. 86%)、7+9(25. 00%)、17+18(3. 57%)和13+16(3. 57%) 4种类型;在Glu-D1位点具有2+12(96. 43%)和5+10(3. 57%) 2种类型。另外,各供试品种(系)共有5种亚基组合,依次为1/7+8/2+12(39. 29%)、Null/7+8/2+12(28. 57%)、Null/7+9/2+12(25. 00%)、1/17+18/5+10(3. 57%)和Null/13+16/2+12(3. 57%)。
In order to learn the genetic basis of quality of the core wheat breeding parents in Southern Zhejiang region,the composition of high molecular weight glutenin subunit of twenty-eight core cultivars (lines) was analyzed using the method of SDS-PAGE. The results showed that there were Null (57. 14%) and 1 (42. 86%) subunit alleles on Glu-A1 loci; 7 + 8 (67. 86%),7 + 9 (25. 00%),17 + 18 (3. 57%) and 13 + 16 (3. 57%) subunit alleles on Glu-B1 loci; 2 + 12 (96. 43%) and 5 + 10 (3. 57%) subunit alleles on Glu-D1 loci. On the other hand,there were five kind of HMW-GS combinations,1/7 + 8/2 + 12 (39. 29%),Null/7 + 8/2 + 12 (28. 57%),Null/7 + 9/2 + 12 (25. 00%),1/17 + 18/5 + 10 (3. 57%) and Null/13 + 16/2 + 12 (3. 57%),respectively.
In order to learn the genetic basis of quality of the core wheat breeding parents in Southern Zhejiang region,the composition of high molecular weight glutenin subunit of twenty-eight core cultivars (lines) was analyzed using the method of SDS-PAGE. The results showed that there were Null (57. 14%) and 1 (42. 86%) subunit alleles on Glu-A1 loci; 7 + 8 (67. 86%),7 + 9 (25. 00%),17 + 18 (3. 57%) and 13 + 16 (3. 57%) subunit alleles on Glu-B1 loci; 2 + 12 (96. 43%) and 5 + 10 (3. 57%) subunit alleles on Glu-D1 loci. On the other hand,there were five kind of HMW-GS combinations,1/7 + 8/2 + 12 (39. 29%),Null/7 + 8/2 + 12 (28. 57%),Null/7 + 9/2 + 12 (25. 00%),1/17 + 18/5 + 10 (3. 57%) and Null/13 + 16/2 + 12 (3. 57%),respectively.
引文
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[2]PAYNE P I.Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality[J].Annual Review of Plant Physiology and Plant Molecular Biology,1987,38(1):141-153.
[3]SHEWRY P R,HALFORD N G,TATHAM A S.High molecular weight subunits of wheat glutenin[J].Journal of Cereal Science,1992,15(2):105-120.
[4]宋建民,刘爱峰,李豪圣,等.小麦麦谷蛋白与面包烘烤品质关系研究进展[C]//中国青年农业科学学术年报.北京:中国农业科学技术出版社,2004:84-89.
[5]毛沛,李宗智,卢少源.小麦遗传资源HMW麦谷蛋白亚基组成及其与面包烘烤品质关系的研究[J].中国农业科学,1995,28(增刊1):22-27.MAO P,LI Z Z,LU S Y.The composition of high molecular weight glutenin subunits of genetic resources of bread wheat and their relationship with bread-making quality[J].Scientia Agricultura Sinica,28(Suppl.1):22-27.(in Chinese with English abstract)
[6]范玉顶,李斯深,孙海艳,等.HMW-GS与北方手工馒头加工品质关系的研究[J].作物学报,2005,31(1):97-101.FAN Y D,LI S S,SUN H Y,et al.Relationship between HMW-GS and making quality of northern style hand-made steamed bread[J].Acta Agronomica Sinica,2005,31(1):97-101.(in Chinese with English abstract)
[7]张影全,魏益民,张波,等.小麦高分子量谷蛋白亚基(HMW-GS)对面条感官质量的影响[J].中国农业科学,2013,46(1):121-129.ZHANG Y Q,WEI Y M,ZHANG B,et al.Influence of high molecular weight glutenin subunits(HMW-GS)on sensory quality properties of Chinese dry noodle[J].Scientia Agricultura Sinica,2013,46(1):121-129.(in Chinese with English abstract)
[8]刘广田,许明辉.普通小麦胚乳谷蛋白亚基的遗传研究[J].中国农业科学,1988,21(1):56-60.LIU G T,XU M H.The inheritance of endosperm glutenin subunit in comon wheat(Triticum aestivum L.)[J].Scientia Agricultura Sinica,1988,21(1):56-60.(in Chinese with English abstract)
[9]张丽琴,刘春雷,杨雪,等.黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析[J].麦类作物学报,2012,32(1):79-82.ZHANG L Q,LIU C L,YANG X,et al.Polymorphism analysis of HMW-GS in new wheat varieties in Huang-huai Wheat Region of China[J].Journal of Triticeae Crops,2012,32(1):79-82.(in Chinese with English abstract)
[10]徐黎黎,颜泽洪,魏育明,等.东方小麦高分子量谷蛋白亚基组成分析[J].四川农业大学学报,2005,23(2):137-141.XU L L,YAN Z H,WEI Y M,et al.Analysis of HMW glutenin subunits in Triticum turanicum Jakubz.[J].Journal of Sichuan Agricultural University,2005,23(2):137-141.(in Chinese with English abstract)
[11]吴远双,宋玉竹.一种考马斯亮蓝G-250快速染色法在SDS-PAGE中的应用[J].光谱实验室,2013,30(6):3109-3113.WU Y S,SONG Y Z.Application of a fast protein staining method with G-250 in SDS-PAGE[J].Chinese Jounaral of Spectroscopy Laboratory,2013,30(6):3109-3113.(in Chinese with English abstract)
[12]郑玉姝,赵朴,赵宏坤.SDS-PAGE染色-脱色方法的改良[J].实验室研究与探索,2007,26(1):41-42.ZHENG Y S,ZHAO P,ZHAO H K.An improved method for staining-destaining of SDS-PAGE[J].Research and Exploration in Laboratory,2007,26(1):41-42.(in Chinese with English abstract)
[13]PAYNE P I,LAWRENCE G J.Catalogue of alleles for the complex gene loci,Glu-A1,Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaplold wheat[J].Cereal Research Communications,1983,11:29-35.
[14]张志清,郑有良,魏育明,等.四川主栽小麦品种高分子量谷蛋白亚基遗传变异分析[J].麦类作物学报,2002,22(1):14-18.ZHANG Z Q,ZHENG Y L,WEI Y M,et al.Genetic variation of HMW-GS among Sichuan wheat cultivars[J].Journal of Triticeae Crops,2002,22(1):14-18.(in Chinese with English abstract)
[15]陈晓杰,王亚娟,申磊,等.西北春麦区小麦地方品种高分子量麦谷蛋白亚基组成分析[J].植物遗传资源学报,2009,10(1):42-45.CHEN X J,WANG Y J,SHEN L,et al.HMW-GS diversity of wheat landraces in northwest spring wheat production area[J].Journal of Plant Genetic Resources,2009,10(1):42-45.(in Chinese with English abstract)
[16]郭志富,衣莹,龙翔宇,等.东北春小麦及部分外引小麦高分子量谷蛋白亚基组成分析[J].华北农学报,2008,23(2):72-76.GOU Z F,YI Y,LONG X Y,et al.Analysis on high-molecular-weight glutenin subunit composition of spring wheat in northeast China and introduced wheat[J].Acta Agriculturae Boreali-Sinica,2008,23(2):72-76.(in Chinese with English abstract)
[17]王红梅,厚毅清,张艳萍,等.甘肃小麦部分种质高分子量麦谷蛋白亚基(HMWGS)遗传变异分析[J].分子植物育种,2009,7(5):873-878.WANG H M,HOU Y Q,ZHANG Y P,et al.Genetic diversity of the collected wheat germplasms in Gansu Province revealed based on HMWGS variation by SDS-PAGE[J].Molecular Plant Breeding,2009,7(5):873-878.(in Chinese with English abstract)
[18]杨子博,顾正中,周羊梅,等.江苏淮北地区小麦品种资源高分子量谷蛋白亚基的组成分析[J].西南农业学报,2015,28(4):1465-1469.YANG Z B,GU Z Z,ZHOU Y M,et al.Composition analysis of HMW-GS in wheat cultivars from Huaibei Region of Jiangsu Province[J].Southwest China Journal of Agricultural Sciences,2015,28(4):1465-1469.(in Chinese with English abstract)
[19]刘永安,潘彬荣,岳高红,等.小麦优质亚基和抗白粉病Pm4a基因聚合体的PCR鉴定[J].浙江农业学报,2016,28(4):545-549.LIU Y A,PAN B R,YUE G H,et al.PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene[J].Acta Agriculturae Zhejiangensis,2016,28(4):545-549.(in Chinese with English abstract)
[20]苏东民.中国馒头分类专家咨询调查研究[J].粮食科技与经济,2006(5):49-51.SU D M.Investigation on the classification of steamed bread in China[J].Grain Science and Technology and Economy,2006(5):49-51.(in Chinese)
[21]施俊生,王仁杯.浙江省小麦品种结构演变的比较分析[J].种子世界,2016(5):19-21.SHU J S,WANG R B.Analysis on the evolution of wheat varieties in Zhejiang Province[J].Seed World,2016(5):19-21.(in Chinese)
[2]PAYNE P I.Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality[J].Annual Review of Plant Physiology and Plant Molecular Biology,1987,38(1):141-153.
[3]SHEWRY P R,HALFORD N G,TATHAM A S.High molecular weight subunits of wheat glutenin[J].Journal of Cereal Science,1992,15(2):105-120.
[4]宋建民,刘爱峰,李豪圣,等.小麦麦谷蛋白与面包烘烤品质关系研究进展[C]//中国青年农业科学学术年报.北京:中国农业科学技术出版社,2004:84-89.
[5]毛沛,李宗智,卢少源.小麦遗传资源HMW麦谷蛋白亚基组成及其与面包烘烤品质关系的研究[J].中国农业科学,1995,28(增刊1):22-27.MAO P,LI Z Z,LU S Y.The composition of high molecular weight glutenin subunits of genetic resources of bread wheat and their relationship with bread-making quality[J].Scientia Agricultura Sinica,28(Suppl.1):22-27.(in Chinese with English abstract)
[6]范玉顶,李斯深,孙海艳,等.HMW-GS与北方手工馒头加工品质关系的研究[J].作物学报,2005,31(1):97-101.FAN Y D,LI S S,SUN H Y,et al.Relationship between HMW-GS and making quality of northern style hand-made steamed bread[J].Acta Agronomica Sinica,2005,31(1):97-101.(in Chinese with English abstract)
[7]张影全,魏益民,张波,等.小麦高分子量谷蛋白亚基(HMW-GS)对面条感官质量的影响[J].中国农业科学,2013,46(1):121-129.ZHANG Y Q,WEI Y M,ZHANG B,et al.Influence of high molecular weight glutenin subunits(HMW-GS)on sensory quality properties of Chinese dry noodle[J].Scientia Agricultura Sinica,2013,46(1):121-129.(in Chinese with English abstract)
[8]刘广田,许明辉.普通小麦胚乳谷蛋白亚基的遗传研究[J].中国农业科学,1988,21(1):56-60.LIU G T,XU M H.The inheritance of endosperm glutenin subunit in comon wheat(Triticum aestivum L.)[J].Scientia Agricultura Sinica,1988,21(1):56-60.(in Chinese with English abstract)
[9]张丽琴,刘春雷,杨雪,等.黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析[J].麦类作物学报,2012,32(1):79-82.ZHANG L Q,LIU C L,YANG X,et al.Polymorphism analysis of HMW-GS in new wheat varieties in Huang-huai Wheat Region of China[J].Journal of Triticeae Crops,2012,32(1):79-82.(in Chinese with English abstract)
[10]徐黎黎,颜泽洪,魏育明,等.东方小麦高分子量谷蛋白亚基组成分析[J].四川农业大学学报,2005,23(2):137-141.XU L L,YAN Z H,WEI Y M,et al.Analysis of HMW glutenin subunits in Triticum turanicum Jakubz.[J].Journal of Sichuan Agricultural University,2005,23(2):137-141.(in Chinese with English abstract)
[11]吴远双,宋玉竹.一种考马斯亮蓝G-250快速染色法在SDS-PAGE中的应用[J].光谱实验室,2013,30(6):3109-3113.WU Y S,SONG Y Z.Application of a fast protein staining method with G-250 in SDS-PAGE[J].Chinese Jounaral of Spectroscopy Laboratory,2013,30(6):3109-3113.(in Chinese with English abstract)
[12]郑玉姝,赵朴,赵宏坤.SDS-PAGE染色-脱色方法的改良[J].实验室研究与探索,2007,26(1):41-42.ZHENG Y S,ZHAO P,ZHAO H K.An improved method for staining-destaining of SDS-PAGE[J].Research and Exploration in Laboratory,2007,26(1):41-42.(in Chinese with English abstract)
[13]PAYNE P I,LAWRENCE G J.Catalogue of alleles for the complex gene loci,Glu-A1,Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaplold wheat[J].Cereal Research Communications,1983,11:29-35.
[14]张志清,郑有良,魏育明,等.四川主栽小麦品种高分子量谷蛋白亚基遗传变异分析[J].麦类作物学报,2002,22(1):14-18.ZHANG Z Q,ZHENG Y L,WEI Y M,et al.Genetic variation of HMW-GS among Sichuan wheat cultivars[J].Journal of Triticeae Crops,2002,22(1):14-18.(in Chinese with English abstract)
[15]陈晓杰,王亚娟,申磊,等.西北春麦区小麦地方品种高分子量麦谷蛋白亚基组成分析[J].植物遗传资源学报,2009,10(1):42-45.CHEN X J,WANG Y J,SHEN L,et al.HMW-GS diversity of wheat landraces in northwest spring wheat production area[J].Journal of Plant Genetic Resources,2009,10(1):42-45.(in Chinese with English abstract)
[16]郭志富,衣莹,龙翔宇,等.东北春小麦及部分外引小麦高分子量谷蛋白亚基组成分析[J].华北农学报,2008,23(2):72-76.GOU Z F,YI Y,LONG X Y,et al.Analysis on high-molecular-weight glutenin subunit composition of spring wheat in northeast China and introduced wheat[J].Acta Agriculturae Boreali-Sinica,2008,23(2):72-76.(in Chinese with English abstract)
[17]王红梅,厚毅清,张艳萍,等.甘肃小麦部分种质高分子量麦谷蛋白亚基(HMWGS)遗传变异分析[J].分子植物育种,2009,7(5):873-878.WANG H M,HOU Y Q,ZHANG Y P,et al.Genetic diversity of the collected wheat germplasms in Gansu Province revealed based on HMWGS variation by SDS-PAGE[J].Molecular Plant Breeding,2009,7(5):873-878.(in Chinese with English abstract)
[18]杨子博,顾正中,周羊梅,等.江苏淮北地区小麦品种资源高分子量谷蛋白亚基的组成分析[J].西南农业学报,2015,28(4):1465-1469.YANG Z B,GU Z Z,ZHOU Y M,et al.Composition analysis of HMW-GS in wheat cultivars from Huaibei Region of Jiangsu Province[J].Southwest China Journal of Agricultural Sciences,2015,28(4):1465-1469.(in Chinese with English abstract)
[19]刘永安,潘彬荣,岳高红,等.小麦优质亚基和抗白粉病Pm4a基因聚合体的PCR鉴定[J].浙江农业学报,2016,28(4):545-549.LIU Y A,PAN B R,YUE G H,et al.PCR identification of the wheat polymers with high quality subunit and powdery mildew resistance Pm4a gene[J].Acta Agriculturae Zhejiangensis,2016,28(4):545-549.(in Chinese with English abstract)
[20]苏东民.中国馒头分类专家咨询调查研究[J].粮食科技与经济,2006(5):49-51.SU D M.Investigation on the classification of steamed bread in China[J].Grain Science and Technology and Economy,2006(5):49-51.(in Chinese)
[21]施俊生,王仁杯.浙江省小麦品种结构演变的比较分析[J].种子世界,2016(5):19-21.SHU J S,WANG R B.Analysis on the evolution of wheat varieties in Zhejiang Province[J].Seed World,2016(5):19-21.(in Chinese)