可降解聚合物抗菌纤维膜的原位制备及性能表征
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摘要
以二氯甲烷、N,N-二甲基甲酰胺为溶剂,以聚乳酸(PLA)为纺丝纤维基体,添加纳米氧化锌(Zn O)和表面活性剂吐温60,利用高压静电纺丝法,原位制备纳米Zn O/PLA复合纤维膜。所得纤维膜中,Zn O分散均匀,纤维平均直径约300 nm,且表面光滑。纺丝纤维的亲水性随着纳米Zn O的增加而大幅提高,断裂强度和延伸率随纳米Zn O含量的增加呈现先上升后下降的趋势。抗菌能力也呈现一定的Zn O含量依赖性。细胞毒性和细胞黏附实验表明,该抗菌纤维膜具有较好的细胞相容性。
The nano-zinc oxide( Zn O,50nm) doped polylactic acid( PLA) nanofibers were fabricated via insitu electrospinning technique. The mixture of dichloromethane( DCM) and n,n-dimethyl formamide( DMF) were pre-prepared for the solvent of PLA,then the nano-zinc oxide particles have been mixed into the solvents mixture.After optimazing the preparation procedure,the Zn O particles were well-distributed in the PLA solvents through observed by TEM. The structure of Zn O / PLA composite nanofibers has been observed by SEM,and the diameter was about 300 nm. Meanwhile,the so spun fibers were smooth and uniform without any bead-like structure The mechanical properties of composite nanofibers have been demonstrated by a tensile test,and the results demonstrated that the breaking strength and elongation rate were increased with the percentage of nano-Zn O. It showed that the addition of nano-Zn O may be an effective way to toughen the PLA nanofibers. Additionally,the hydrophilicity of the compound nanofibers has been recorded through a contact angle apparatus. It showed that the hydrophilicity of compound nanofibers were decreased monotonically with the increasing nano-Zn O content,obviously. Then,the cytotoxicity and the antibacterial properties have also been examined. These results suggested that the Zn O / PLA nanofibers were safe to cells and SEM observation also showed that cells had spread well onto the fiber mats with a high level of cell / scaffold interaction; this was demonstrated by the numerous pseudopodia and cell secretions that emerged. Finally,the ability to antibacterial was investigated through a Bacteriostatic ring experiment which showed that the antibacterial property had a Zn O-concentration dependent manner.
The nano-zinc oxide( Zn O,50nm) doped polylactic acid( PLA) nanofibers were fabricated via insitu electrospinning technique. The mixture of dichloromethane( DCM) and n,n-dimethyl formamide( DMF) were pre-prepared for the solvent of PLA,then the nano-zinc oxide particles have been mixed into the solvents mixture.After optimazing the preparation procedure,the Zn O particles were well-distributed in the PLA solvents through observed by TEM. The structure of Zn O / PLA composite nanofibers has been observed by SEM,and the diameter was about 300 nm. Meanwhile,the so spun fibers were smooth and uniform without any bead-like structure The mechanical properties of composite nanofibers have been demonstrated by a tensile test,and the results demonstrated that the breaking strength and elongation rate were increased with the percentage of nano-Zn O. It showed that the addition of nano-Zn O may be an effective way to toughen the PLA nanofibers. Additionally,the hydrophilicity of the compound nanofibers has been recorded through a contact angle apparatus. It showed that the hydrophilicity of compound nanofibers were decreased monotonically with the increasing nano-Zn O content,obviously. Then,the cytotoxicity and the antibacterial properties have also been examined. These results suggested that the Zn O / PLA nanofibers were safe to cells and SEM observation also showed that cells had spread well onto the fiber mats with a high level of cell / scaffold interaction; this was demonstrated by the numerous pseudopodia and cell secretions that emerged. Finally,the ability to antibacterial was investigated through a Bacteriostatic ring experiment which showed that the antibacterial property had a Zn O-concentration dependent manner.
引文
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2 代佑果,寸英丽.皮肤替代物研究及应用进展.中国美容整形外科杂志,2008;19(2):132—135Dai Youguo,Cun Yingli.The progress of development and application for skin substitute.Chinese Journal of Aesthetic and Plastic Surgery,2008;19(2):132—135
3 谈敏,李临生.敷料与人工皮肤技术研究进展.化学通报,2000;(11):7—13Tan Min,Li Linsheng.The development of research of wound dressing and artifical skin.Chemistry,2000;(11):7—13
4 胡玎玎,吴振飞,刘小琨,等.皮肤组织工程支架材料的研究进展.高分子通报,2012;10:7—12Hu Dingding,Wu Zhenfei,Liu Xiaokun,et al.The development of scaffolds for skin tissue engineering.Polymer Bulletin,2012;10:7 —12
5 Daley W P,Peters S B,Larsen M.Extracellular matrix dynamicsin development and regenerative medicine.J Cell Sci,2008;121:255 —264
6 曹成波,吴克安,王振芳,等.皮肤组织工程支架材料的研究现状及其发展.功能材料,2004;(35):2314—2316Cao Chengbo,Wu Kean,Wang Zhenfang,et al.The present situation and development in scaffolds of skin tissue engineering.Journal of Functional Materials,2004;(35):2314—2316
7 Venugopal J,Low S,Choon A T,et al.Interaction of cellsand nanofiber scaffolds in tissue engineering.J Biomed Mater Res B Appl Biomater,2008;84:34—48
8 Ma Z,Kotaki M,Inai R,et al.Potential of nanofiber matrixas tissueengineering scaffolds.Tissue Eng,2005;11:101—119
9 Vasita R,Katti D S.Nanofibers and their applications in tissue engineering.Int J Nanomed,2006;1:15—30