Bim与BCSG1蛋白在乳腺癌中的表达及临床意义研究
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摘要
目的探究Bcl-2相互作用细胞凋亡调节因子(Bim)与乳腺癌特异性基因1(BCSG1)蛋白在乳腺癌中的表达及临床意义。方法选取我院2015年1月至2017年2月期间收治的60例乳腺癌患者,通过外科手术收集60例患者乳腺癌组织以及癌旁组织,通过免疫组织化学染色检测两种组织中Bim与BCSG1蛋白表达情况,分析不同分期、不同分化程度、不同病理、不同直径和是否转移情况下Bim与BCSG1蛋白表达差异。结果免疫组织化学染色结果显示:Bim与BCSG1在乳腺癌组织中高表达,而在癌旁组织中低表达,比较差异有统计学意义(P<0.05);肿瘤直径≥4cm、低分化程度、浸润性乳腺癌、Ⅲ-Ⅳ期和远处转移的乳腺癌患者,Bim与BCSG1阳性率明显高于肿瘤直径<4cm、高度分化、中度分化、非浸润性乳腺癌、Ⅰ-Ⅱ期和非转移的乳腺癌患者(P<0.05)。结论 Bim和BCSG1蛋白在乳腺癌组织中高表达,且表达水平与肿瘤大小、分期、浸润、分化程度和转移性密切相关。
Objective To observe the expression and clinical significance of Bcl-2 interacting mediator of cell death(Bim) and breast cancer specific gene 1(BCSG1) protein in breast cancer.Methods 60 cases of breast cancer patients admitted in our hospital during January 2015-February 2016 were selected.Pericancer tissues and breast cancer tissues were collected by operation.The expression of Bim and BCSG1 protein in patients with different stages,different degrees of differentiation,different pathology,different diameters and metastasis were detected through immunohistochemical staining.Results Immunohistochemical staining showed that the expression levels of Bim and BCSG1 in breast cancer tissues were high,but their expression levels inpericancer tissues were low(P < 0.05).When the stage was Ⅲ-Ⅳ,the tumor showed large size(> 4 cm),lowdegree differentiation,benign breast cancer and distant metastasis.The positive ratio of Bim and BCSG1 was significantly higher than those of the patients with noninvasive,tumor diameter less than 4 cm,moderate degree differentiation,high degree differentiation,distant metastasis andⅠandⅡstage(P < 0.05).Conclusion The expression of Bim and BCSG1 protein were significantly higher in breast cancer patients,and it was closely correlated with stage,tumor size,pathological type,differentiation and metastasis.
Objective To observe the expression and clinical significance of Bcl-2 interacting mediator of cell death(Bim) and breast cancer specific gene 1(BCSG1) protein in breast cancer.Methods 60 cases of breast cancer patients admitted in our hospital during January 2015-February 2016 were selected.Pericancer tissues and breast cancer tissues were collected by operation.The expression of Bim and BCSG1 protein in patients with different stages,different degrees of differentiation,different pathology,different diameters and metastasis were detected through immunohistochemical staining.Results Immunohistochemical staining showed that the expression levels of Bim and BCSG1 in breast cancer tissues were high,but their expression levels inpericancer tissues were low(P < 0.05).When the stage was Ⅲ-Ⅳ,the tumor showed large size(> 4 cm),lowdegree differentiation,benign breast cancer and distant metastasis.The positive ratio of Bim and BCSG1 was significantly higher than those of the patients with noninvasive,tumor diameter less than 4 cm,moderate degree differentiation,high degree differentiation,distant metastasis andⅠandⅡstage(P < 0.05).Conclusion The expression of Bim and BCSG1 protein were significantly higher in breast cancer patients,and it was closely correlated with stage,tumor size,pathological type,differentiation and metastasis.
引文
[1]富琦,王皓,韩冬,等.乳腺癌术后患者化疗前后中医证候变化的临床研究.北京中医药,2012,31(1):4-6.
[2]南永刚,刘新芸,施常备,等.血清CA153和HER-2联合检测在乳腺癌诊断中的研究.标记免疫分析与临床,2016,23(7):731-733.
[3]GEHRMANN M,SCHMIDT M,BRASE J C,et al.Prediction of paclitaxel resistance in breast cancer:is CYP1B1*3 a new factor of influence?Pharmacogenomics,2017,9(7):969-974.
[4]ORLANDO G,LAW P J,PALIN K,et al.Variation at 2q35(PNKD and TMBIM1)influences colorectal cancer risk and identifies a pleiotropic effect with inflammatory bowel disease.Hum Mol Genet,2016,25(11):2349-2359.
[5]BURGESS P M,PRINCE G D.Non-unique stratal geometries:implications for sequence stratigraphic interpretations.Basin Res,2015,27(3):351-365.
[6]中华人民共和国卫生部医政司制定.乳腺癌诊疗规范(2011年版).中国实用外科杂志,2011,31(10):902-907.
[7]SIU A L.Screening for breast cancer:U.S.Preventive Services Task Force recommendation statement.Ann Intern Med,2016,164(4):279-296.
[8]卓小丽,李诗运,戴儒奇.18F-FDG PET-CT在确定乳腺癌术前诊断与分期中的临床价值.标记免疫分析与临床,2016,23(4):442-445,469.
[9]BUTTA A,MACLENNAN K,FLANDERS K C,et al.Induction of transforming growth factorβ1 in human breast cancer in vivo following tamoxifen treatment.Cancer Res,2017,52(15):4261-4264.
[10]王蕾,邬晓敏,吕庆,等.乳腺癌术后辅助化疗患者癌因性疲劳的中医证候特点分析.北京中医药,2016,35(7):649-652.
[11]CHALABI N,DELORT L,LE C L,et al.Gene signature of breast cancer cell lines treated with lycopene.Pharmacogenomics,2017,7(5):663-672.
[12]KRISTINA L,INGVAR A,ALDANA R,et al.Performance of oneview breast tomosynthesis as a stand-alone breast cancer screening modality:results from the Malm9 Breast Tomosynthesis Screening Trial,a population-based study.Eur Radiol,2016,26(1):184-190.
[13]李泉源,魏涧,严礼琴,等.己糖激酶-Ⅱ在乳腺癌组织中的表达及其临床意义.实用老年医学,2015,29(7):580-582.
[14]HASEGAWA H,BISSONNETTE R P,GILLINGS M,et al.Induction of apoptosis by HBI‐8000 in adult T‐cell leukemia/lymphoma is associated with activation of Bim and NLRP3.Cancer Sci,2016,107(8):1124-1133.
[15]WU D W,CHEN C Y,CHU C L,et al.Paxillin confers resistance to tyrosine kinase inhibitors in EGFR-mutant lung cancers via modulating BIM and Mcl-1 protein stability.Oncogene,2016,35(5):621-630.
[16]WEBER A,HEINLEIN M,DENGJEL J,et al.The deubiquitinase Usp27x stabilizes the BH3-only protein Bim and enhances apoptosis.EMBO Rep,2016,17(5):724-738.
[17]SCHULER F,BAUMGARTNER F,KLEPSCH V,et al.The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland.Cell Death Differ,2016,23(1):41-51.
[18]GAMBICHLER T,ROOMS I,SCHOLL L,et al.BH3-only protein Bim predicts advanced stage of cutaneous melanoma.J Eur Acad Dermatol Venereal,2016,30(11):1926-1929.
[19]WANG W H,ZHOU C H,DING J,et al.Immune effect and safety evaluation of vaccine prepared by dendritic cells modified by r AAVcarrying BCSG1 gene.Gene Ther,2016,23(12):839-846.
[20]宋士军,王全志.BCSG1蛋白在乳腺癌组织中的表达情况及临床意义.解放军医药杂志,2016,28(3):76-80.
[2]南永刚,刘新芸,施常备,等.血清CA153和HER-2联合检测在乳腺癌诊断中的研究.标记免疫分析与临床,2016,23(7):731-733.
[3]GEHRMANN M,SCHMIDT M,BRASE J C,et al.Prediction of paclitaxel resistance in breast cancer:is CYP1B1*3 a new factor of influence?Pharmacogenomics,2017,9(7):969-974.
[4]ORLANDO G,LAW P J,PALIN K,et al.Variation at 2q35(PNKD and TMBIM1)influences colorectal cancer risk and identifies a pleiotropic effect with inflammatory bowel disease.Hum Mol Genet,2016,25(11):2349-2359.
[5]BURGESS P M,PRINCE G D.Non-unique stratal geometries:implications for sequence stratigraphic interpretations.Basin Res,2015,27(3):351-365.
[6]中华人民共和国卫生部医政司制定.乳腺癌诊疗规范(2011年版).中国实用外科杂志,2011,31(10):902-907.
[7]SIU A L.Screening for breast cancer:U.S.Preventive Services Task Force recommendation statement.Ann Intern Med,2016,164(4):279-296.
[8]卓小丽,李诗运,戴儒奇.18F-FDG PET-CT在确定乳腺癌术前诊断与分期中的临床价值.标记免疫分析与临床,2016,23(4):442-445,469.
[9]BUTTA A,MACLENNAN K,FLANDERS K C,et al.Induction of transforming growth factorβ1 in human breast cancer in vivo following tamoxifen treatment.Cancer Res,2017,52(15):4261-4264.
[10]王蕾,邬晓敏,吕庆,等.乳腺癌术后辅助化疗患者癌因性疲劳的中医证候特点分析.北京中医药,2016,35(7):649-652.
[11]CHALABI N,DELORT L,LE C L,et al.Gene signature of breast cancer cell lines treated with lycopene.Pharmacogenomics,2017,7(5):663-672.
[12]KRISTINA L,INGVAR A,ALDANA R,et al.Performance of oneview breast tomosynthesis as a stand-alone breast cancer screening modality:results from the Malm9 Breast Tomosynthesis Screening Trial,a population-based study.Eur Radiol,2016,26(1):184-190.
[13]李泉源,魏涧,严礼琴,等.己糖激酶-Ⅱ在乳腺癌组织中的表达及其临床意义.实用老年医学,2015,29(7):580-582.
[14]HASEGAWA H,BISSONNETTE R P,GILLINGS M,et al.Induction of apoptosis by HBI‐8000 in adult T‐cell leukemia/lymphoma is associated with activation of Bim and NLRP3.Cancer Sci,2016,107(8):1124-1133.
[15]WU D W,CHEN C Y,CHU C L,et al.Paxillin confers resistance to tyrosine kinase inhibitors in EGFR-mutant lung cancers via modulating BIM and Mcl-1 protein stability.Oncogene,2016,35(5):621-630.
[16]WEBER A,HEINLEIN M,DENGJEL J,et al.The deubiquitinase Usp27x stabilizes the BH3-only protein Bim and enhances apoptosis.EMBO Rep,2016,17(5):724-738.
[17]SCHULER F,BAUMGARTNER F,KLEPSCH V,et al.The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland.Cell Death Differ,2016,23(1):41-51.
[18]GAMBICHLER T,ROOMS I,SCHOLL L,et al.BH3-only protein Bim predicts advanced stage of cutaneous melanoma.J Eur Acad Dermatol Venereal,2016,30(11):1926-1929.
[19]WANG W H,ZHOU C H,DING J,et al.Immune effect and safety evaluation of vaccine prepared by dendritic cells modified by r AAVcarrying BCSG1 gene.Gene Ther,2016,23(12):839-846.
[20]宋士军,王全志.BCSG1蛋白在乳腺癌组织中的表达情况及临床意义.解放军医药杂志,2016,28(3):76-80.