HS-SPME-GC-MS联用分析羌活、防风药对配伍前后挥发性组分变化规律
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摘要
目的比较羌活、防风药对配伍前后挥发性组分变化。方法采用顶空固相微萃取法(HS-SPME)与气相色谱-质谱联用(GC-MS)法,单因素考察并确定最佳萃取条件,测定羌活、防风以及羌活、防风药对的挥发性化学成分,通过面积归一法确定各成分含量。结果最佳萃取条件为取样量0.5 g,萃取温度80℃,萃取时间15 min,解析时间3 min。测出92个羌活挥发性成分,71个防风挥发性成分,150个药对挥发性成分,分别占挥发性成分总面积的70.90%、88.52%和81.48%。药对中新增43种新的成分,单味药中有34种成分在药对中未能检测出,44种共有成分含量减少,10种共有成分含量增加。结论羌活、防风药对的挥发性成分大部分来自于羌活,羌活、防风配伍后有新的活性成分产生,该药对配伍可能具有协同增效作用。
Objective To analyze the volatile components in Notopterygll rhizome et radix,Saposhnikovia radix,and their couplet medicines. Methods The optimum extraction conditions were determined by single factor test.Headspace solid-phase microextraction(HS-SPME)was used in combination with gas chromatography-mass spectrometry(GC-MS)to determine volatile chemicals from Notopterygll rhizome et radix,Saposhnikovia radix,and their couplet medicines. Contents of ingredients were determined by area normalization. Results The optimum extraction conditions were as follows: sample size 0.5 g, extraction temperature 80°C, extraction time 15 min,analysis time 3 min. Ninety two volatile components of Notopterygll rhizome et radix were detected, 71 volatile components of Saposhnikovia radix were detected,and 150 volatile components of couplet medicines were detected,accounting for 70.90%,88.52%,and 81.48% of the total volatile components,respectively. There were 43 new ingredients in the drug pair, and 34 of the herbs detected before combination were missing in the drug pair.Contents of 44 shared components were reduced, and those of 10 common components were increased after the combination. Conclusion The volatile components in the drug pair mainly come from Notopterygll rhizome et radix;and there are new active ingredients produced by the combination of Notopterygll rhizome et radix and Saposhnikovia radix,which may have certain guiding significance for clinical use.
Objective To analyze the volatile components in Notopterygll rhizome et radix,Saposhnikovia radix,and their couplet medicines. Methods The optimum extraction conditions were determined by single factor test.Headspace solid-phase microextraction(HS-SPME)was used in combination with gas chromatography-mass spectrometry(GC-MS)to determine volatile chemicals from Notopterygll rhizome et radix,Saposhnikovia radix,and their couplet medicines. Contents of ingredients were determined by area normalization. Results The optimum extraction conditions were as follows: sample size 0.5 g, extraction temperature 80°C, extraction time 15 min,analysis time 3 min. Ninety two volatile components of Notopterygll rhizome et radix were detected, 71 volatile components of Saposhnikovia radix were detected,and 150 volatile components of couplet medicines were detected,accounting for 70.90%,88.52%,and 81.48% of the total volatile components,respectively. There were 43 new ingredients in the drug pair, and 34 of the herbs detected before combination were missing in the drug pair.Contents of 44 shared components were reduced, and those of 10 common components were increased after the combination. Conclusion The volatile components in the drug pair mainly come from Notopterygll rhizome et radix;and there are new active ingredients produced by the combination of Notopterygll rhizome et radix and Saposhnikovia radix,which may have certain guiding significance for clinical use.
引文
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[2]张亚军,谢放,张育,等.羌活化学成分的研究进展[J].湖南农业科学,2017,(4):124-126.
[3]时博,史晶晶.羌活药性及功效应用考证[J].中医学报,2017,32(7):1239-1241.
[4]杨景明,姜华,孟祥才.中药防风质量评价的现状与思考[J].中药材,2016,39(7):1678-1681.
[5]周涛.药对甘草-麻黄、桂枝-羌活和防风-羌活化学成分研究[D].长沙:中南大学,2009.
[6]陈帅华,余莲芳,李晓如,等.药对防风-羌活与其单味药挥发油共有组分的分析[J].中南大学学报(自然科学版),2010,41(2):440-445.
[7]李佳钰,吴雨杭,李昊原,等.固相微萃取技术与应用研究进展[J].山东化工,2016,45(1):43-45.
[8]傅若农.固相微萃取(SPME)近几年的发展[J].分析试验室,2015,34(5):602-620.
[9]徐刚,史茗歌,吴明红,等.固相微萃取的原理及应用[J].上海大学学报(自然科学版),2013,19(4):368-373.
[10]刘梦菲,江汉美,肖宇硕,等.HS-SPME-GC-MS联用技术分析不同产地艾叶挥发性成分[J].中国实验方剂学杂志,2018(10):79-89.
[11]宋玉玲,胡坪,赵诗怡,等.HS-SPME/GC-MS结合化学计量学鉴别不同菌种发酵虫草产品及挥发性成分分析[J].药物分析杂志,2018,38(01):67-78.
[12]赵一帆,张东,杨立新,等.HS-SPME-GC-MS测定洋甘菊不同部位挥发性成分[J].中国实验方剂学杂志,2018,24(02):69-73.
[13]吴克刚,罗敏婷,魏浩.8种植物精油对肠道常见微生物体外抑菌效果的研究[J].现代食品科技,2017,33(6):139-147.
[14]姚元.3种芳香植物单方及其复方精油的成分分析及抗真菌作用[D].乌鲁木齐:新疆农业大学,2012.
[15]王雅连.MeJA诱导条件下白木香根的转录组分析研究[D].广州:广州中医药大学,2016.
[16]戴静波,张雅娟,周俊慧.防风挥发油的气相色谱指纹图谱研究[J].中国中医药信息杂志,2013,20(5):55-57.
[17]粱臣艳,覃洁萍,陈玉萍,等.不同产地防风挥发油的GC-MS分析[J].中国实验方剂学杂志,2012,18(8):80-83.