桑叶-苦瓜混合粉对正常小鼠血糖血脂和抗氧化能力的影响
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摘要
探讨桑叶-苦瓜混合粉对正常小鼠血糖、血脂、抗氧化能力的影响,为评价桑叶-苦瓜混合粉是否适用于正常人群提供参考。用不同质量浓度的桑叶-苦瓜混合液灌胃正常小鼠30 d,测定小鼠体质量、血糖浓度、糖耐量、脂代谢和抗氧化的相关指标。结果表明:与对照组比较,桑叶-苦瓜混合粉显著抑制正常小鼠体质量增加,随灌胃质量浓度增加该作用趋于平稳;当灌胃0.20 g/mL桑叶-苦瓜混合粉时,对血糖浓度无明显影响,雌、雄小鼠血糖曲线下面积下降率分别为14.18%、15.69%,肝糖原含量分别增加1.93、1.98倍;血清总胆固醇浓度分别下降17.19%、18.92%,甘油三酯浓度分别下降15.00%、16.79%,低密度脂蛋白胆固醇浓度分别下降41.67%、45.83%,高密度脂蛋白胆固醇浓度分别上升64.52%、72.22%;肝脏丙二醛含量分别下降36.71%、37.73%,超氧化物歧化酶活力分别上升41.77%、48.41%,谷胱甘肽过氧化物酶活力分别上升29.04%、27.21%。桑叶-苦瓜混合粉对正常小鼠的血糖、血脂水平和抗氧化功能有调节作用,不会对健康造成不利影响。
This study was intended to investigate the effect of mulberry leaf and bitter melon powder blend on blood glucose,blood lipid and antioxidant status in normal mice. The mice were administered with four different concentrations of the blend for 30 days. Body mass, blood glucose, glucose tolerance, lipid metabolism and antioxidant indicators were measured during the experimental period. As a result, the blend exerted a significant inhibitory effect on body mass gain in mice compared with the control group and the effect remained unchanged at higher concentrations. Compared with the normal control group, the blend at 0.20 g/mL had no significant effect on blood glucose levels, although it resulted in a decrease in glucose area under the curve(AUC) of 14.18% and 15.69%, an increase in hepatic glycogen level of 2.93 and 2.98 times, a reduction in serum total cholesterol(TC) of 17.19% and 18.92%, a decrease in triglycerides(TG) of 15.00% and 16.79%, a decrease in low-density lipoprotein cholesterol(LDL-C) of 41.67% and 45.83% and an increase in high-density lipoprotein cholesterol(HDL-C) of 64.52% and 72.22%, as well as an increase in hepatic glycogen of 193% and 198%, a reduction in malondialdehyde(MDA) level of 36.71% and 37.73%, an increase in superoxide dismutase(SOD) activity of 41.77%and 48.41% and an increase in glutathione peroxidase(GSH-Px) activity of 29.04% and 27.21% for female and male mice,respectively. These results suggested that mulberry leaf and bitter melon powder blend can regulate body mass, blood glucose,glucose tolerance, lipid metabolism and antioxidant status without causing any adverse health effects in normal mice.
This study was intended to investigate the effect of mulberry leaf and bitter melon powder blend on blood glucose,blood lipid and antioxidant status in normal mice. The mice were administered with four different concentrations of the blend for 30 days. Body mass, blood glucose, glucose tolerance, lipid metabolism and antioxidant indicators were measured during the experimental period. As a result, the blend exerted a significant inhibitory effect on body mass gain in mice compared with the control group and the effect remained unchanged at higher concentrations. Compared with the normal control group, the blend at 0.20 g/mL had no significant effect on blood glucose levels, although it resulted in a decrease in glucose area under the curve(AUC) of 14.18% and 15.69%, an increase in hepatic glycogen level of 2.93 and 2.98 times, a reduction in serum total cholesterol(TC) of 17.19% and 18.92%, a decrease in triglycerides(TG) of 15.00% and 16.79%, a decrease in low-density lipoprotein cholesterol(LDL-C) of 41.67% and 45.83% and an increase in high-density lipoprotein cholesterol(HDL-C) of 64.52% and 72.22%, as well as an increase in hepatic glycogen of 193% and 198%, a reduction in malondialdehyde(MDA) level of 36.71% and 37.73%, an increase in superoxide dismutase(SOD) activity of 41.77%and 48.41% and an increase in glutathione peroxidase(GSH-Px) activity of 29.04% and 27.21% for female and male mice,respectively. These results suggested that mulberry leaf and bitter melon powder blend can regulate body mass, blood glucose,glucose tolerance, lipid metabolism and antioxidant status without causing any adverse health effects in normal mice.
引文
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[13]SAEED F M,ARSHAD M S,NISA M U,et al.Hypoglycemic and hypolipidemic effects of different parts and formulations of bitter gourd(Momordica charantia)[J].Lipids in Health and Disease,2017,16(1):211.DOI:10.1186/s12944-017-0602-7.
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[21]方太松,朱帅,丁晓雯,等.桑叶超微粉奶茶降脂功效的评价[J].现代食品科技,2017,33(12):23-28.
[22]李玲,秦樱瑞,丁晓雯,等.桑叶-苦瓜混合提取液的体外抗氧化性研究[J].食品科技,2016,41(12):194-198.DOI:10.136684/j.cnki.spkj.2016.12.039.
[23]杨敏,丁晓雯,秦樱瑞,等.桑叶-苦瓜混合粉对糖尿病小鼠血脂的影响[J].现代食品科技,2017,33(8):12-17;40.DOI:10.13982/j.mfst.1673-9078.2017.8.003.
[24]彭晓蝶,秦樱瑞,黄先智,等.桑叶-苦瓜混合粉对糖尿病小鼠的降糖作用[J].现代食品科技,2017,33(4):31-37.DOI:10.13982/j.mfst.1673-9078.2017.4.006.
[25]秦樱瑞,曾艺涛,杨娟,等.干燥方式对桑叶降糖活性成分含量的影响[J].食品科学,2015,36(17):71-76.DOI:10.7506/spkx1002-6630-201517014.
[26]秦樱瑞,黄先智,曾艺涛,等.干燥方法对苦瓜降糖成分含量的影响[J].食品科学,2015,36(15):56-61.DOI:10.7506/spkx1002-6630-201515012.
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