血清S100B蛋白对肺癌脑转移的诊断价值
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摘要
目的检测肺癌脑转移患者血清S100B蛋白水平,探讨S100B作为肺癌脑转移相关血清标志物的可能性。方法采用ELISA分别检测111例肺癌脑转移患者(肺癌脑转移组)、62例肺癌未转移患者(肺癌无转移组)、33例肺癌其他部位转移患者(肺癌其他转移组)、38例健康体检者(健康对照组)血清S100B水平,应用SPSS19.0分析组间差异并制作ROC曲线。结果肺癌脑转移组S100B水平高于肺癌无转移组、其他转移组和健康对照组,差异有统计学意义(P<0.05);肺癌无转移组与其他转移组、健康对照组S100B水平差异无统计学意义(P>0.05)。S100B水平与病理分型、肿瘤年限、年龄、性别、吸烟史均无关(P>0.05)。S100B诊断肺癌脑转移的AUC为0.701,敏感度为45.0%,特异性为91.0%。结论肺癌脑转移患者血清S100B蛋白水平升高,S100B蛋白诊断肺癌脑转移具有一定的敏感度和较高的特异性,具有一定的临床价值,可以作为肺癌脑转移的血清标志物。
Objective To detect the levels the serum S100 B protein in patients with brain metastasis of lung cancer,and explore the possibility of using S100 B as a serum marker associated with brain metastasis of lung cancer.Methods The level of serum S100 B protein was detected by ELISA respectively in 111 cases with brain metastasis of lung cancer(brain metastasis of lung cancer group),62 cases without metastasis of lung cancer(lung cancer without metastasis group),33 cases with metastasis of other parts of lung cancer(lung cancer with other parts metastasis group) and 38 healthy volunteers(control group).We analyzed the difference between the groups and made ROC Curve with SPSS19.0.Results(1) Brain metastasis of lung cancer group showed higher S100 B protein level than the lung cancer without metastasis group,the lung cancer with other parts metastasis group and the healthy control group(P<0.05),but there were no significant changes in the lung cancer without metastasis group,the lung cancer with other parts metastasis group and the healthy control group(P > 0.05).(2) S100 B protein level was not related to pathological type,tumor age,age,sex and smoking history.(3) The measurement showed an AUC of 0.701.And the sensitivity and specificity of S100 B protein in the diagnosis of brain metastasis of lung cancer were 45.0% and 91.0%,respectively.Conclusion Serum S100 B protein was up-regulated in patients with brain metastasis of lung cancer and has a certain sensitivity and specificity in the diagnosis of brain metastasis of lung cancer.It has certain clinical value and can be used as a serum marker for brain metastasis of lung cancer.
Objective To detect the levels the serum S100 B protein in patients with brain metastasis of lung cancer,and explore the possibility of using S100 B as a serum marker associated with brain metastasis of lung cancer.Methods The level of serum S100 B protein was detected by ELISA respectively in 111 cases with brain metastasis of lung cancer(brain metastasis of lung cancer group),62 cases without metastasis of lung cancer(lung cancer without metastasis group),33 cases with metastasis of other parts of lung cancer(lung cancer with other parts metastasis group) and 38 healthy volunteers(control group).We analyzed the difference between the groups and made ROC Curve with SPSS19.0.Results(1) Brain metastasis of lung cancer group showed higher S100 B protein level than the lung cancer without metastasis group,the lung cancer with other parts metastasis group and the healthy control group(P<0.05),but there were no significant changes in the lung cancer without metastasis group,the lung cancer with other parts metastasis group and the healthy control group(P > 0.05).(2) S100 B protein level was not related to pathological type,tumor age,age,sex and smoking history.(3) The measurement showed an AUC of 0.701.And the sensitivity and specificity of S100 B protein in the diagnosis of brain metastasis of lung cancer were 45.0% and 91.0%,respectively.Conclusion Serum S100 B protein was up-regulated in patients with brain metastasis of lung cancer and has a certain sensitivity and specificity in the diagnosis of brain metastasis of lung cancer.It has certain clinical value and can be used as a serum marker for brain metastasis of lung cancer.
引文
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14李季林,盛罗平,陈仁辉,等.S100B蛋白浓度的测定对颅脑损伤分型与判断预后的意义[J].中华全科医学,2013,11(10):1509-1510,1626
15 Reid H,Rahul J.Conduits between cancer cells and astrocytes[J].Neurosurgery,2016,79(4):16-17
16 Dai W,Zhu H,Chen G,et al.Orchestration of the crosstalk between astrocytes and cancer cells affects the treatment and prognosis of lung cancer sufferers with brain metastasis[J].J Thorac Dis,2016,8(11):E1450-E1454
17 Malladi S,Macalinao DG,Jin X,et al.Metastatic latency and immune evasion through autocrine inhibition of wnt[J].Cell,2016,165(1):45-60
18梅芬,卢忠心.肺癌脑转移与血清肿瘤标志物水平的关系[J].临床与实验病理学杂志,2016,32(1):96-98
19石远凯,孙燕,于金明,等.中国肺癌脑转移诊治专家共识(2017年版)[J].中国肺癌杂志,2017,20(1):1-13
2 Owonikoko TK,Arbiser J,Zelnak A,et al.Current approaches to the treatment of metastatic brain tumours[J].Nat Rev Clin Oncol,2014,11(4):203-222
3 Preusser M,Capper D,Ilhan-Mutlu A,et al.Brain metastases:pathobiology and emerging targeted therapies[J].Acta Neuropathol,2012,123(2):205-222
4 Soffietti R,Cornu P,Delattre JY,et al.EFNS Guidelines on diagnosis and treatment of brain metastases:report of an EFNS Task Force[J].Eur J Neurol,2006,13(7):674-681
5 Liu W,Zhao J,Wei YJ.Association between brain metastasis from lung cancer and the serum level of myelin basic protein[J].Exp Ther Med,2015,9(3):1048-1050
6 Weide B,Richter S,Büttner P,et al.Serum S100B,lactate dehydrogenase and brain metastasis are prognostic factors in patients with distant melanoma metastasis and systemic therapy[J].PLo S One,2013,8(11):e81624
7 Lippi G,Cervellin G.Protein S100B:from cancer diagnostics to the evaluation of mild traumatic brain injury[J].Clin Chem Lab Med,2016,54(5):703-705
8 Koch MW,George S,Wall W,et al.Serum NSE level and disability progression in multiple sclerosis[J].J Neurol Sci,2015,350(1-2):46-50
9 Shadi A,Kamran H,Mehrdad T,et al.Reducing head computed tomography after mild traumatic brain injury:screening value of clinical findings and S100B protein levels[J].Brain INJ,2016,30(2):172-178
10 Jonsson H,Johnsson P,Hoglund P,et al.Elimination of S100B and renal function after cardiac surgery[J].J Cardiothorac Vasc Anesth,2000,14(6):698-701
11 Heidari K,Asadollahi S,Jamshidian M,et al.Prediction of neuropsychological outcome after mild traumatic brain injury using clinical parameters,serum S100B protein and findings on computed tomography[J].Brain INJ,2015,29(1):33-40
12 Thelin EP,Nelson DW,Bellander BM.Secondary peaks of S100B in serum relate to subsequent radiological pathology in traumatic brain injury[J].Neurocrit Care,2014,20(2):217-229
13何文根,刘瑶,金玲江.S100B蛋白与脑血管病的研究进展[J].医学研究杂志,2013,42(12):147-149
14李季林,盛罗平,陈仁辉,等.S100B蛋白浓度的测定对颅脑损伤分型与判断预后的意义[J].中华全科医学,2013,11(10):1509-1510,1626
15 Reid H,Rahul J.Conduits between cancer cells and astrocytes[J].Neurosurgery,2016,79(4):16-17
16 Dai W,Zhu H,Chen G,et al.Orchestration of the crosstalk between astrocytes and cancer cells affects the treatment and prognosis of lung cancer sufferers with brain metastasis[J].J Thorac Dis,2016,8(11):E1450-E1454
17 Malladi S,Macalinao DG,Jin X,et al.Metastatic latency and immune evasion through autocrine inhibition of wnt[J].Cell,2016,165(1):45-60
18梅芬,卢忠心.肺癌脑转移与血清肿瘤标志物水平的关系[J].临床与实验病理学杂志,2016,32(1):96-98
19石远凯,孙燕,于金明,等.中国肺癌脑转移诊治专家共识(2017年版)[J].中国肺癌杂志,2017,20(1):1-13