白及组织培养初步研究
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摘要
以成熟白及蒴果为外植体,通过对白及无菌萌发、无菌苗增殖及无菌苗生根的研究,对白及组织培养进行了初步探讨。结果表明,不添加任何激素的1/2MS培养基对种子萌发效果较好;诱导无菌苗增殖的较适组合为MS+6-BA 1.00 mg/L+NAA 0.05 mg/L+KT 1.00 mg/L,无菌苗增殖系数达到1.83;白及无菌苗生根过程中添加番茄汁出现枯萎现象,但生根率没有影响,较适生根培养基为1/2MS+NAA 1.0 mg/L+GA30.5 mg/L+番茄汁100 g/L。
Taking ripe capsular fruits of Bletilla striata as the test material, the rapid propagation and tissue culture were obtained by studying the seed germination, multiplication and rooting of aseptic seedlings. Results showed that 1/2 MS culture medium without hormones was suitable for seed germination. The appropriate culture medium for aseptic seedling multiplication was MS+6-BA 1.00 mg/L+NAA 0.05 mg/L+KT 1.00 mg/L and the rate of proliferation reached to 1.83. Fusarium wilt occured when tomato juice was added in the rooting process of aseptic seedling of Bletilla striata, but the rooting rate hadn't been affected. The optimum culture medium for the rooting of aseptic seeding of Bletilla striata was 1/2 MS +NAA 1.0 mg/L+ GA30.5 mg/L+ tomato juice 100 g/L.
Taking ripe capsular fruits of Bletilla striata as the test material, the rapid propagation and tissue culture were obtained by studying the seed germination, multiplication and rooting of aseptic seedlings. Results showed that 1/2 MS culture medium without hormones was suitable for seed germination. The appropriate culture medium for aseptic seedling multiplication was MS+6-BA 1.00 mg/L+NAA 0.05 mg/L+KT 1.00 mg/L and the rate of proliferation reached to 1.83. Fusarium wilt occured when tomato juice was added in the rooting process of aseptic seedling of Bletilla striata, but the rooting rate hadn't been affected. The optimum culture medium for the rooting of aseptic seeding of Bletilla striata was 1/2 MS +NAA 1.0 mg/L+ GA30.5 mg/L+ tomato juice 100 g/L.
引文
[1]任风鸣,刘艳,李滢,等.白及属药用植物的资源分布及繁育[J].中草药,2016,47(24):4478-4487.
[2]赵荣英,杨晓霞.鹤庆白族地区民间药用植物资源初步调查[J].大理学院学报,2006,5(10):25-27.
[3]陈巧玲,杨国才,程群,等.白及组织培养的建立与快速繁殖研究[J].湖北农业科学,2017,56(24):4807-4810.
[4]蒋俊,陈红霞,汤兴利,等.基于中医药美白理论的白及研发思考[J].中草药,2007,48(11):2313-2320.
[5]郭顺兴,徐锦堂.白芨种子染菌萌发过程中细胞超微结构变化的研究[J].植物学报,1990,32(8):594-598.
[6]徐德林,储世润,李树刚.四种天然添加物对白及组培苗诱导的影响[J].西南师范大学学报(自然科学版),2005,40(10):181-186.
[7]陶刚,朱英,刘作易,等.野生黄花白芨的组织快繁及分子鉴定[J].种子,2008,27(8):22-24.
[8]张建霞,付志惠,李洪林,等.白芨胚发育与种子萌发的关系[J].亚热带植物科学,2005,34(4):32-35.
[9]CHUNG J D,CHUN C K,SUH J H. Asymbiotic germination of seeds of Bletilla striata. II. Effect of peptone,sucrose and agar concentration and pH value on seedling growth[J]. Journal of the Korean Society for Horticultural Science,1983,24(3):243-248.
[10]张燕,黎斌,李思锋.不同培养基上白芨的种子萌发与幼苗形态发生[J].西北植物学报,2009,29(8):1584-1589.
[11]叶静,郑晓君,管常东,等.白芨的无菌萌发与组织培养[J].云南大学学报(自然科学版),2010,32(S1):422-425.
[12]和寿星,徐中志,薛润光,等.白及无菌播种育苗技术[J].云南农业科技,2010(6):38-39.
[13]吕晋慧,孔冬梅.园艺植物组织培养[M].北京:中国农业科学技术出版社,2008:24-26.
[14]田英翠,袁雄强.白芨组织培养快繁技术研究[J].江苏农业科学,2006(4):75-77.
[15]宋晓丹,陈晓玲,尚丽,等.白及种子试管高频萌发的应用研究[J].中国现代中药,2014,16(9):751-754.
[16]袁宁,何俊蓉,何锐,等.白芨组培快繁育苗技术研究[J].西南农业学报,2009,22(3):781-785.
[2]赵荣英,杨晓霞.鹤庆白族地区民间药用植物资源初步调查[J].大理学院学报,2006,5(10):25-27.
[3]陈巧玲,杨国才,程群,等.白及组织培养的建立与快速繁殖研究[J].湖北农业科学,2017,56(24):4807-4810.
[4]蒋俊,陈红霞,汤兴利,等.基于中医药美白理论的白及研发思考[J].中草药,2007,48(11):2313-2320.
[5]郭顺兴,徐锦堂.白芨种子染菌萌发过程中细胞超微结构变化的研究[J].植物学报,1990,32(8):594-598.
[6]徐德林,储世润,李树刚.四种天然添加物对白及组培苗诱导的影响[J].西南师范大学学报(自然科学版),2005,40(10):181-186.
[7]陶刚,朱英,刘作易,等.野生黄花白芨的组织快繁及分子鉴定[J].种子,2008,27(8):22-24.
[8]张建霞,付志惠,李洪林,等.白芨胚发育与种子萌发的关系[J].亚热带植物科学,2005,34(4):32-35.
[9]CHUNG J D,CHUN C K,SUH J H. Asymbiotic germination of seeds of Bletilla striata. II. Effect of peptone,sucrose and agar concentration and pH value on seedling growth[J]. Journal of the Korean Society for Horticultural Science,1983,24(3):243-248.
[10]张燕,黎斌,李思锋.不同培养基上白芨的种子萌发与幼苗形态发生[J].西北植物学报,2009,29(8):1584-1589.
[11]叶静,郑晓君,管常东,等.白芨的无菌萌发与组织培养[J].云南大学学报(自然科学版),2010,32(S1):422-425.
[12]和寿星,徐中志,薛润光,等.白及无菌播种育苗技术[J].云南农业科技,2010(6):38-39.
[13]吕晋慧,孔冬梅.园艺植物组织培养[M].北京:中国农业科学技术出版社,2008:24-26.
[14]田英翠,袁雄强.白芨组织培养快繁技术研究[J].江苏农业科学,2006(4):75-77.
[15]宋晓丹,陈晓玲,尚丽,等.白及种子试管高频萌发的应用研究[J].中国现代中药,2014,16(9):751-754.
[16]袁宁,何俊蓉,何锐,等.白芨组培快繁育苗技术研究[J].西南农业学报,2009,22(3):781-785.