三氯生对斑马鱼微核率和核异常率的影响
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摘要
为了探讨三氯生(TCS)对鱼类遗传毒性,通过半静态水质接触染毒法对斑马鱼进行染毒,设置对照组和三个不同浓度组(0.017 mg/L、0.034 mg/L、0.068 mg/L)的TCS处理组,染毒28 d后,测定斑马鱼外周血红细胞的微核率和核异常率。结果显示:TCS各处理组红细胞的微核率和核异常率显著升高(P<0.01),且随着TCS浓度的增大而增大,呈现一定的剂量-效应关系。以上结果表明:TCS对斑马鱼具有潜在的遗传毒性。
To explore the genotoxicity of triclosan to fish, zebrafish were exposed to blank control and three different doses of triclosan solution(0.017 mg/L, 0.034 mg/L, 0.068 mg/L) by semi-static water contracting con-tamination method for 28 days. Then, we studied the effects of triclosan on blood erythrocytes micronucleus and nuclear anomalies rate in the zebrafish. It was showed that the micronuclei and nuclear anomalies were significant-ly increased with the increasing of triclosan concentration(P<0.01). In addition, there was a dose-effect relation-ship between triclosan dose and micronuclei, nuclear anomalies. These findings suggested that the triclosan had a potential genotoxicity on zebrafish.
To explore the genotoxicity of triclosan to fish, zebrafish were exposed to blank control and three different doses of triclosan solution(0.017 mg/L, 0.034 mg/L, 0.068 mg/L) by semi-static water contracting con-tamination method for 28 days. Then, we studied the effects of triclosan on blood erythrocytes micronucleus and nuclear anomalies rate in the zebrafish. It was showed that the micronuclei and nuclear anomalies were significant-ly increased with the increasing of triclosan concentration(P<0.01). In addition, there was a dose-effect relation-ship between triclosan dose and micronuclei, nuclear anomalies. These findings suggested that the triclosan had a potential genotoxicity on zebrafish.
引文
[1]宋燕.掺杂石墨烯气体扩散电极电—Fenton法处理三氯生模拟废水的研究[D].大连:大连理工大学,2013.
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[6]刘飞,刘旭昊,王凡,等.三氯生对红白鲫的急性毒性及遗传毒性研究[J].水生态学杂志,2016,37(5):82-86.
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[9]Oliveira R,Domingues I,Koppe Grisolia C,et al.Effects of triclosan on zebrafish early-life stages and adults.Environ Sci Pollut Res Int,2009,16(6):679-688.
[10]刘楠楠,吕碧平,厉以强,等.稀有鮈鲫血液红细胞微核试验方法研究[J].中国环境监测,2012,28(3):121-126.
[11]龚婧婧,黄海涛,米其利,等.微核技术研究进展[J].生物技术通报,2012,3:49-56.
[12]王杨科,王琦,路宏朝.镉对鲫鱼和泥鳅外周红细胞微核的诱导效应[J].江苏农业科学,2012,40(3):203-205.
[13]李倩,胡廷尖,王雨辰,等.Cu2+对泥鳅外周血红细胞微核及核异常的影响[J].水产养殖,2011,32(10):44-47.
[14]耿德贵,张大生,程伟,等.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响[J].动物学杂志,2000,35(1):12-16.
[15]曹光群,陈忠良,宋启军.三氯生的光降解产物分析及相关日化产品的安全性研究[J].分析试验室,2008,27(8):58-61.
[16]张曙光.黄河重金属污染物对鱼类的致突变性研究[J].环境科学研究,1996,9(3):51-57.
[17]胡国成,甘炼,吴天送,等.硫丹对斑马鱼的毒性效应[J].动物学杂志,2008,43(4):1-6.
[2]Dhillon G S,Kaur S,Pulicharla R,et al.Triclosan:current status,occurrence,environmental risks and bioaccumulation potential[J].International Journal of Environmental Research&Public Health,2015,12(5):5657-5684.
[3]Corteza F S,Seabra Pereira C D,et al.Biological effects of environmentally relevant concentrations of the pharmaceutical triclosan in the marine mussel Perna perna(Linnaeus,1758)[J].Environmental Pollution,2012,168(5):227-233.
[4]周雪飞,陈家斌,周世兵,等.三氯生检测方法的建立与优化[J].中国给水排水,2010,26(12):126-129.
[5]高海萍,周雪飞,张亚雷,等.三氯生对水生生物的毒性效应研究进展[J].环境化学,2012,31(8):1145-1150.
[6]刘飞,刘旭昊,王凡,等.三氯生对红白鲫的急性毒性及遗传毒性研究[J].水生态学杂志,2016,37(5):82-86.
[7]Rodricks JV,Swenberg JA,Borzelleca JF,et al.Tri closan:a critical review of the experimental data and development of margins of safety for consumer products.Critical Reviews in Toxicology,2010,40(5):422–484.
[8]李汝,逯南南,李梅等.斑马鱼生物学效应的研究进展及在水质监测中的应用[J].供水技术,2014,8(6):7-12.
[9]Oliveira R,Domingues I,Koppe Grisolia C,et al.Effects of triclosan on zebrafish early-life stages and adults.Environ Sci Pollut Res Int,2009,16(6):679-688.
[10]刘楠楠,吕碧平,厉以强,等.稀有鮈鲫血液红细胞微核试验方法研究[J].中国环境监测,2012,28(3):121-126.
[11]龚婧婧,黄海涛,米其利,等.微核技术研究进展[J].生物技术通报,2012,3:49-56.
[12]王杨科,王琦,路宏朝.镉对鲫鱼和泥鳅外周红细胞微核的诱导效应[J].江苏农业科学,2012,40(3):203-205.
[13]李倩,胡廷尖,王雨辰,等.Cu2+对泥鳅外周血红细胞微核及核异常的影响[J].水产养殖,2011,32(10):44-47.
[14]耿德贵,张大生,程伟,等.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响[J].动物学杂志,2000,35(1):12-16.
[15]曹光群,陈忠良,宋启军.三氯生的光降解产物分析及相关日化产品的安全性研究[J].分析试验室,2008,27(8):58-61.
[16]张曙光.黄河重金属污染物对鱼类的致突变性研究[J].环境科学研究,1996,9(3):51-57.
[17]胡国成,甘炼,吴天送,等.硫丹对斑马鱼的毒性效应[J].动物学杂志,2008,43(4):1-6.