盐碱胁迫下阿拉善荒漠区2种沙生灌木幼苗体内脯氨酸变化规律的研究
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摘要
以采集自阿拉善荒漠的2种旱生灌木黑果枸杞和唐古特白刺为材料,研究不同浓度(0、50、100、200、300 mmol/L) Na Cl、KCl、Na2CO3和Na HCO3处理,对幼苗叶片中脯氨酸积累的影响。结果表明:观测期内,不同浓度、不同类型的盐碱胁迫均显著增加了脯氨酸在黑果枸杞和唐古特白刺幼苗叶片中的积累。相同胁迫处理下,黑果枸杞幼苗中脯氨酸的累积水平高于唐古特白刺。
Seeds of two different plants,Lycium ruthenicum and Nitraria tangutorum,were sampled from Alagxa desert to explore the change of proline content in leaves of their seedlings under different saline-alkali stresses.NaCl,KCl,Na_2CO_3 and NaHCO_3 solutions,each in four different gradient concentrations(50,100,200,300mmol/L),were supplemented to pots containing seedlings of one month old. The results show that proline accumulation in seedlings of Lycium ruthenicum and Nitraria tangutorum was significantly enhanced after saline-alkali stress with different concentrations and types. The seedlings of Lycium ruthenicum can accumulate more proline than the ones of Nitraria tangutorum under the same stress condition.
Seeds of two different plants,Lycium ruthenicum and Nitraria tangutorum,were sampled from Alagxa desert to explore the change of proline content in leaves of their seedlings under different saline-alkali stresses.NaCl,KCl,Na_2CO_3 and NaHCO_3 solutions,each in four different gradient concentrations(50,100,200,300mmol/L),were supplemented to pots containing seedlings of one month old. The results show that proline accumulation in seedlings of Lycium ruthenicum and Nitraria tangutorum was significantly enhanced after saline-alkali stress with different concentrations and types. The seedlings of Lycium ruthenicum can accumulate more proline than the ones of Nitraria tangutorum under the same stress condition.
引文
[1] Zhu J K. Plant salt tolerance[J]. Trends in Plant Science,2001,6(2):66-71.
[2] Wang L,Guo Z,Zhang Y,et al. Characterization of Lh SorP5CS,a gene catalyzing proline synthesis in Oriental hybrid lily Sorbonne:molecular modelling and expression analysis[J]. Botanical Studies,2017,58(1):10.
[3] Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379.
[4] Rejeb B K,Abdelly C,Savoure A. How reactive oxygen species and proline face stress together[J]. Plant Physiology and Biochemistry,2014,80:278-284.
[5] Verbruggen N,Hermans C. Proline accumulation in plants:a review[J]. Amino Acids,2008,35(4):753-759.
[6]马荣梅,马彦军. Na Cl胁迫对黑果枸杞叶片生理指标的影响[J].甘肃农业大学学报,2017,52(4):110-117.
[7]范小峰,杨颖丽,程转霞. Na Cl胁迫下唐古特白刺愈伤组织生理生化变化研究[J].干旱地区农业研究,2009,27(3):203-207.
[8] Fabro G,Kovacs I,Pavet V,et al. Proline accumulation and At P5CS2 gene activation are induced by plant-pathogen incompatible interactions in Arabidopsis[J]. Molecular Plant-Microbe Interactions,2004,17(4):343-350.
[9] Bojorquez-Quintal E,Velarde-Buendia A,Ku-Gonzalez A,et al. Mechanisms of salt tolerance in habanero pepper plants(Capsicum chinense Jacq.):Proline accumulation,ions dynamics and sodium root-shoot partition and compartmentation[J]. Frontiers in Plant Science,2014,5:605.
[10] Zheng L,Dang Z,Li H,et al. Isolation and characterization of a Delta1-pyrroline-5-carboxylate synthetase(Nt P5CS)from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue[J]. Molecular Biology Reports,2014,41(1):563-572.
[11] Jain R K,Dhawan R S,Sharma D R,et al. Salt-tolerance and proline accumulation:a comparative study in salt-tolerant and wild type cultured cells of eggplant[J]. Plant Cell Reports,1987,6(5):382-384.
[12] Singh T N,Aspinall D,Paleg L G. Proline accumulation and varietal adaptability to drought in barley:a potential metabolic measure of drought resistance[J]. Nature New Biology,1972,236(67):188-190.
[13] Deng G,Liang J,Xu D,et al. The relationship between proline content,the expression level of P5CS(delta(1)-pyrroline-5-carboxylate synthetase),and drought tolerance in Tibetan hulless barley(Hordeum vulgare var. nudum)[J]. Russian Journal of Plant Physiology,2013,60(5):693-700.
[14] Bhaskara G B,Yang T H,Verslues P E. Dynamic proline metabolism:importance and regulation in water limited environments[J]. Frontiers in Plant Science,2015,6:484.
[2] Wang L,Guo Z,Zhang Y,et al. Characterization of Lh SorP5CS,a gene catalyzing proline synthesis in Oriental hybrid lily Sorbonne:molecular modelling and expression analysis[J]. Botanical Studies,2017,58(1):10.
[3] Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379.
[4] Rejeb B K,Abdelly C,Savoure A. How reactive oxygen species and proline face stress together[J]. Plant Physiology and Biochemistry,2014,80:278-284.
[5] Verbruggen N,Hermans C. Proline accumulation in plants:a review[J]. Amino Acids,2008,35(4):753-759.
[6]马荣梅,马彦军. Na Cl胁迫对黑果枸杞叶片生理指标的影响[J].甘肃农业大学学报,2017,52(4):110-117.
[7]范小峰,杨颖丽,程转霞. Na Cl胁迫下唐古特白刺愈伤组织生理生化变化研究[J].干旱地区农业研究,2009,27(3):203-207.
[8] Fabro G,Kovacs I,Pavet V,et al. Proline accumulation and At P5CS2 gene activation are induced by plant-pathogen incompatible interactions in Arabidopsis[J]. Molecular Plant-Microbe Interactions,2004,17(4):343-350.
[9] Bojorquez-Quintal E,Velarde-Buendia A,Ku-Gonzalez A,et al. Mechanisms of salt tolerance in habanero pepper plants(Capsicum chinense Jacq.):Proline accumulation,ions dynamics and sodium root-shoot partition and compartmentation[J]. Frontiers in Plant Science,2014,5:605.
[10] Zheng L,Dang Z,Li H,et al. Isolation and characterization of a Delta1-pyrroline-5-carboxylate synthetase(Nt P5CS)from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue[J]. Molecular Biology Reports,2014,41(1):563-572.
[11] Jain R K,Dhawan R S,Sharma D R,et al. Salt-tolerance and proline accumulation:a comparative study in salt-tolerant and wild type cultured cells of eggplant[J]. Plant Cell Reports,1987,6(5):382-384.
[12] Singh T N,Aspinall D,Paleg L G. Proline accumulation and varietal adaptability to drought in barley:a potential metabolic measure of drought resistance[J]. Nature New Biology,1972,236(67):188-190.
[13] Deng G,Liang J,Xu D,et al. The relationship between proline content,the expression level of P5CS(delta(1)-pyrroline-5-carboxylate synthetase),and drought tolerance in Tibetan hulless barley(Hordeum vulgare var. nudum)[J]. Russian Journal of Plant Physiology,2013,60(5):693-700.
[14] Bhaskara G B,Yang T H,Verslues P E. Dynamic proline metabolism:importance and regulation in water limited environments[J]. Frontiers in Plant Science,2015,6:484.