5种中药对猪霍乱沙门菌的抑菌作用
|
摘要
为研究黄连、黄芩、五倍子、黄柏和鱼腥草提取物对猪霍乱沙门菌的抑菌效果,采用超声波处理,乙醇回流,水浴加热的方法提取5种中药的有效成分,采用2倍稀释法分别测定提取物对猪霍乱沙门菌的最小抑菌质量浓度(mini-mum inhibitory concentration,MIC)和最小杀菌质量浓度(minimum bactericidal concentration,MBC),采用肉汤稀释棋盘法,测定提取物对猪霍乱沙门菌的联合抑菌效果。结果显示,黄连单独抑菌时的MIC值较其他中药的MIC值低,且与黄芩、五倍子联合用药时MIC值由7.81×103 mg/L降到3.91×103 mg/L。而黄芩与黄连、黄柏、五倍子、鱼腥草联合用药时MIC值均降低。黄芩与其他4种药联合用药后抑菌效果增强,且与五倍子联合用药时FICI值小于0.5表现为协同作用,并且2种药物的MIC降低明显。结果表明,黄连、黄芩、五倍子对猪霍乱沙门菌有较好的抑菌作用,黄连、黄芩、五倍子联用时,抑菌效果为协同或相加作用;黄芩与鱼腥草联用时抑菌效果为无关作用;黄柏与鱼腥草联用时抑菌效果也为无关作用。
In order to evaluate combined antibacterial effects of five Chinese traditional medicines extracts including of coptischinensis,baikal skullcap,gallnut,golden cypress and houttuynia cordata against Salmonella choleraesuis.Ultrasonic processing,ethanolre fluxing and water bath heating methods were used to extract the effective components of the five Chinese traditional medicines.Double tube dilution method was used to determine the minimal inhibitory concentrations(MIC)and minimal bactericidal concentrations(MBC)of the extracts against Salmonella choleraesuis.Broth chessboard method was used to determine the combined effect of extracts from traditional Chinese medicines on Salmonella choleraesuis.The MIC value of coptischinensis was significantly lower than that of other traditional Chinese medicine,when combined with scutellaria baicalensis and gallnut the MIC value decreased from 7.81×103 to 3.91×103 mg/L;when radix scutellariae combined with coptischinensis,gallnut and houttuynia cordata the MIC value decreased,the results showed that the bacteriostatic effect of radix scutellariae and other 4 drugs was enhanced,when combined with gallnut the FICI value was less than 0.5,and the MIC of the two drugs decreased significantly.The results show that coptischinensis,scutellaria baicalensis and gallachinensis have good antibacterial activity against Salmonella choleraesuis,when combined with rhizoma coptidis and radix scutellariae,the inhibitory effects were synergistic or additive;when combined with houttuynia cordata and radix scutellariae,the inhibitory effects were irrelevant;when combined with houttuynia cordata and golden cypress,the inhibitory effects were irrelevant.
In order to evaluate combined antibacterial effects of five Chinese traditional medicines extracts including of coptischinensis,baikal skullcap,gallnut,golden cypress and houttuynia cordata against Salmonella choleraesuis.Ultrasonic processing,ethanolre fluxing and water bath heating methods were used to extract the effective components of the five Chinese traditional medicines.Double tube dilution method was used to determine the minimal inhibitory concentrations(MIC)and minimal bactericidal concentrations(MBC)of the extracts against Salmonella choleraesuis.Broth chessboard method was used to determine the combined effect of extracts from traditional Chinese medicines on Salmonella choleraesuis.The MIC value of coptischinensis was significantly lower than that of other traditional Chinese medicine,when combined with scutellaria baicalensis and gallnut the MIC value decreased from 7.81×103 to 3.91×103 mg/L;when radix scutellariae combined with coptischinensis,gallnut and houttuynia cordata the MIC value decreased,the results showed that the bacteriostatic effect of radix scutellariae and other 4 drugs was enhanced,when combined with gallnut the FICI value was less than 0.5,and the MIC of the two drugs decreased significantly.The results show that coptischinensis,scutellaria baicalensis and gallachinensis have good antibacterial activity against Salmonella choleraesuis,when combined with rhizoma coptidis and radix scutellariae,the inhibitory effects were synergistic or additive;when combined with houttuynia cordata and radix scutellariae,the inhibitory effects were irrelevant;when combined with houttuynia cordata and golden cypress,the inhibitory effects were irrelevant.
引文
[1]徐引弟,郭爱珍,贾爱卿,等.猪霍乱沙门菌的快速分离鉴定[J].畜牧与兽医,2007,39(2):8-10.
[2]CHIU C H,CHUANG C H,CHIU S,et al.Salmonella enterica serotype Choleraesuis infections in pediatric patients[J].Pediatrics,2006,117(6):e1193.
[3]刘海侠,凌天星,马国辅,等.沙门菌在鸡群中的危害[J].中国禽业导航,2008,25(8):34.
[4]谢宝年.鸡白痢的危害与控制措施[J].养殖技术顾问,2011,39(9):2-3.
[5]宋连敏,梁立生,宋丙奎,等.鸡白痢沙门菌病的诊断与防治[J].家禽科学,2009,30(12):30-31.
[6]徐成刚.兽用抗生素对公共卫生的影响[J].动物医学进展,2002,23(6):45-48.
[7]TAM M,NINDIM M.Determination ofβ-lactam residues in food stuffs of animal origin using supported liquid membrane extraction and liquid chromatography-mass spectrometry[J].Food Chem,2007,99(2):836-844.
[8]阳元娥,谭伟.夏枯草总黄酮超声提取工艺[J].食品研究与开发,2011,32(4):31-34.
[9]刘丹,汤海峰,张三奇,等.虎杖中有效成分提取方法的研究[J].中成药,2007,29(4):516-521.
[10]徐燕,典灵辉,陆江赢,等.德庆首乌二苯乙烯苷提取的研究[J].辽宁化工,2011,10(1):4-6.
[11]付戈妍,付克,王洪光,等.超声波提取紫花地丁总黄酮及含量测定[J].内蒙古民族大学学报,2008,23(5):512-513.
[12]王雷,富丽静,于翔,等.中草药五倍子、诃子有效成分提取工艺的研究[J].水生态学杂志,2009,2(1):135-137.
[13]舒凌玲,陈雅,吴畏,等.奥硝唑联用醋酸氯己定对白色念珠菌的体外抗菌活性考察[J].中国药业,2005,14(5):27-28.
[14]周文中,毕可东,邹本革,等.氟苯尼考与盐酸土霉素体外联合抑菌效果研究[J].中国畜牧兽医,2011,38(7):201-202.
[2]CHIU C H,CHUANG C H,CHIU S,et al.Salmonella enterica serotype Choleraesuis infections in pediatric patients[J].Pediatrics,2006,117(6):e1193.
[3]刘海侠,凌天星,马国辅,等.沙门菌在鸡群中的危害[J].中国禽业导航,2008,25(8):34.
[4]谢宝年.鸡白痢的危害与控制措施[J].养殖技术顾问,2011,39(9):2-3.
[5]宋连敏,梁立生,宋丙奎,等.鸡白痢沙门菌病的诊断与防治[J].家禽科学,2009,30(12):30-31.
[6]徐成刚.兽用抗生素对公共卫生的影响[J].动物医学进展,2002,23(6):45-48.
[7]TAM M,NINDIM M.Determination ofβ-lactam residues in food stuffs of animal origin using supported liquid membrane extraction and liquid chromatography-mass spectrometry[J].Food Chem,2007,99(2):836-844.
[8]阳元娥,谭伟.夏枯草总黄酮超声提取工艺[J].食品研究与开发,2011,32(4):31-34.
[9]刘丹,汤海峰,张三奇,等.虎杖中有效成分提取方法的研究[J].中成药,2007,29(4):516-521.
[10]徐燕,典灵辉,陆江赢,等.德庆首乌二苯乙烯苷提取的研究[J].辽宁化工,2011,10(1):4-6.
[11]付戈妍,付克,王洪光,等.超声波提取紫花地丁总黄酮及含量测定[J].内蒙古民族大学学报,2008,23(5):512-513.
[12]王雷,富丽静,于翔,等.中草药五倍子、诃子有效成分提取工艺的研究[J].水生态学杂志,2009,2(1):135-137.
[13]舒凌玲,陈雅,吴畏,等.奥硝唑联用醋酸氯己定对白色念珠菌的体外抗菌活性考察[J].中国药业,2005,14(5):27-28.
[14]周文中,毕可东,邹本革,等.氟苯尼考与盐酸土霉素体外联合抑菌效果研究[J].中国畜牧兽医,2011,38(7):201-202.