辽宁地区野生和栽培羊肚菌ITS序列及ISSR多态性分析
|
摘要
针对采集到的15株栽培羊肚菌样品和14株野生羊肚菌样品进行ITS序列分析和ISSR多态性分析,通过构建系统发育树和遗传聚类分析图谱,发现辽宁地区主栽品种为黑色系的六妹羊肚菌(Morchella sextelata,Mel-6)和梯棱羊肚菌(Morchella importuna,Mel-10),同时还有少量的七妹羊肚菌(Morchella septimelata,Mel-7);野生羊肚菌样品属于黄色系的Morchella sp.(Mes-6)和Morchella sp.(Mes-9)。结果表明辽宁地区羊肚菌栽培品种与野生羊肚菌品种具有明显的遗传差异性,为实现本地野生羊肚菌资源的合理利用和人工栽培研究提供参考。
15 strains of cultivated Morchella sp. and 14 samples of wild Morchella sp. were collected, which were analyzed by ITS sequence analysis, ISSR polymorphism and construction of phylogenetic trees and genetic clustering analysis maps. The results showed the main varieties in Liaoning province were Morchella sextelata(Mel-6) and Morchella importuna(Mel-10), and there were a small amount of Morchella septimelata(Mel-7). The wild Morchella sp. samples belong to yellow strain were Morchella sp.(Mes-6) and Morchella sp.(Mes-9). Genetic differences was obvious between cultivated Morchella and wild varieties in Liaoning province, which provide reference for the rational utilization of wild morel resources and artificial cultivation research.
15 strains of cultivated Morchella sp. and 14 samples of wild Morchella sp. were collected, which were analyzed by ITS sequence analysis, ISSR polymorphism and construction of phylogenetic trees and genetic clustering analysis maps. The results showed the main varieties in Liaoning province were Morchella sextelata(Mel-6) and Morchella importuna(Mel-10), and there were a small amount of Morchella septimelata(Mel-7). The wild Morchella sp. samples belong to yellow strain were Morchella sp.(Mes-6) and Morchella sp.(Mes-9). Genetic differences was obvious between cultivated Morchella and wild varieties in Liaoning province, which provide reference for the rational utilization of wild morel resources and artificial cultivation research.
引文
[1]Hibbett D,Binder M,Bischoff J,et al. A higher-level phylogenetic classification of the fungi[J]. Mycological Research,2007,111(5):509-547.
[2]刘波. 中国药用真菌[M]. 太原:山西人民出版社,1984.
[3]郭浩. 辽宁的羊肚菌资源[J]. 食用菌,1992(2):4.
[4]张元霞,姜玉乙,郭元涛,等. 辽宁东部山区珍贵野生食用菌[J]. 辽宁林业科技,2006(1):21-25.
[5]李红. 辽阳地区野生羊肚菌及其菌丝培养试验[J]. 食用菌,2015(2):13-14.
[6]王红,曹君,吕立涛,等. 辽宁康平地区两种羊肚菌调查与鉴定[J]. 园艺与种苗,2018(4):17-20.
[7]Wipf D,Munch JC,Botton B,et al. DNA polymorphism in morels:complete sequences of the interal transcribed spacer of genes coding for rRNA in Morchella exculenta(yellow morel)and Morchella conica(black morel)[J]. Applied and Environmental Microbiology,1996,62(9):3541-3543.
[8]Wipf D,Fribourg A,Munch JC,et al. Diversity of the internal transcribed spacer of rDNA in morels[J]. Canadian Journal of Microbiology,1999,45(9):769-778.
[9]杜习慧,赵琪,杨祝良. 羊肚菌属的分子系统发育和生物地理研究[A]. 2012年中国菌物学会学术年会会议摘要集[C]. 北京:中国菌物学会,2012.
[10]Du X H,Zhao Q,Yang ZL,et al. Morchella ITS rDNA phylogenetics-How well do ITS rDNA sequences differentiate species of true morels(Morchella)?[J]. Mycologia,2012,104(6):1351-1368.
[11]Du XH,Zhao Q,O’Donnell K,et al. Multigene molecular phylogenetics reveals true morels(Morchella)are especially species-rich in China[J]. Funal genetics and biology,2012,49(6):455-469.
[12]闫晓雪,时小东,张国珍,等. 西南地区部分野生羊肚菌ITS序列鉴定及生境分析[J]. 微生物学杂志,2016,36(6):48-53.
[13]陈璇,冯丽娜,孙伟明,等. 秦皇岛地区4株野生和栽培羊肚菌的鉴定[J]. 河北科技师范学院学报,2018,32(1):20-24.
[14]熊川,李小林,李强,等. 四川秋季发生的两种羊肚菌生境调查与鉴定[J]. 菌物学报,2016,35(1):29-38.
[15]王龙,秦鹏,王生荣,等. 甘肃甘南野生羊肚菌rDNA的ITS序列分析[J]. 草原与草坪,2014,34(6):41-44.
[16]王波,鲜灵. 人工栽培羊肚菌的鉴定[J]. 西南农业学报,2013,26(5):1988-1991.
[17]刘文丛,张建博,桂明英,等. 滇西北地区5种羊肚菌遗传多样性的ISSR分析[J]. 中国食用菌,2011,30(4):38-42.
[18]魏巍,余梦瑶,徐晓燕,等. 不同羊肚菌菌株的遗传差异性与胃粘膜保护作用的比较研究[J]. 食品科学,2014,35(7):160-163.
[19]武冬梅. 新疆野生羊肚菌分子系统学和遗传多样性研究[D]. 北京:中国农业大学大学,2015.
[20]杜习慧,赵琪,杨祝良. 羊肚菌的多样性、演化历史及栽培研究进展[J]. 菌物学报,2014,33(2):183-197.
[2]刘波. 中国药用真菌[M]. 太原:山西人民出版社,1984.
[3]郭浩. 辽宁的羊肚菌资源[J]. 食用菌,1992(2):4.
[4]张元霞,姜玉乙,郭元涛,等. 辽宁东部山区珍贵野生食用菌[J]. 辽宁林业科技,2006(1):21-25.
[5]李红. 辽阳地区野生羊肚菌及其菌丝培养试验[J]. 食用菌,2015(2):13-14.
[6]王红,曹君,吕立涛,等. 辽宁康平地区两种羊肚菌调查与鉴定[J]. 园艺与种苗,2018(4):17-20.
[7]Wipf D,Munch JC,Botton B,et al. DNA polymorphism in morels:complete sequences of the interal transcribed spacer of genes coding for rRNA in Morchella exculenta(yellow morel)and Morchella conica(black morel)[J]. Applied and Environmental Microbiology,1996,62(9):3541-3543.
[8]Wipf D,Fribourg A,Munch JC,et al. Diversity of the internal transcribed spacer of rDNA in morels[J]. Canadian Journal of Microbiology,1999,45(9):769-778.
[9]杜习慧,赵琪,杨祝良. 羊肚菌属的分子系统发育和生物地理研究[A]. 2012年中国菌物学会学术年会会议摘要集[C]. 北京:中国菌物学会,2012.
[10]Du X H,Zhao Q,Yang ZL,et al. Morchella ITS rDNA phylogenetics-How well do ITS rDNA sequences differentiate species of true morels(Morchella)?[J]. Mycologia,2012,104(6):1351-1368.
[11]Du XH,Zhao Q,O’Donnell K,et al. Multigene molecular phylogenetics reveals true morels(Morchella)are especially species-rich in China[J]. Funal genetics and biology,2012,49(6):455-469.
[12]闫晓雪,时小东,张国珍,等. 西南地区部分野生羊肚菌ITS序列鉴定及生境分析[J]. 微生物学杂志,2016,36(6):48-53.
[13]陈璇,冯丽娜,孙伟明,等. 秦皇岛地区4株野生和栽培羊肚菌的鉴定[J]. 河北科技师范学院学报,2018,32(1):20-24.
[14]熊川,李小林,李强,等. 四川秋季发生的两种羊肚菌生境调查与鉴定[J]. 菌物学报,2016,35(1):29-38.
[15]王龙,秦鹏,王生荣,等. 甘肃甘南野生羊肚菌rDNA的ITS序列分析[J]. 草原与草坪,2014,34(6):41-44.
[16]王波,鲜灵. 人工栽培羊肚菌的鉴定[J]. 西南农业学报,2013,26(5):1988-1991.
[17]刘文丛,张建博,桂明英,等. 滇西北地区5种羊肚菌遗传多样性的ISSR分析[J]. 中国食用菌,2011,30(4):38-42.
[18]魏巍,余梦瑶,徐晓燕,等. 不同羊肚菌菌株的遗传差异性与胃粘膜保护作用的比较研究[J]. 食品科学,2014,35(7):160-163.
[19]武冬梅. 新疆野生羊肚菌分子系统学和遗传多样性研究[D]. 北京:中国农业大学大学,2015.
[20]杜习慧,赵琪,杨祝良. 羊肚菌的多样性、演化历史及栽培研究进展[J]. 菌物学报,2014,33(2):183-197.