药食同源作物苦荞分子生物学研究进展
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摘要
苦荞作为一种药食同源作物,具有极高的经济效益和研究价值.随着现代高通量测序技术的发展,为苦荞分子生物学研究提供了方便和快捷的途径.从分子标记和遗传多样性、组学和功能基因等方面对苦荞分子生物学研究进行了综述.研究认为,苦荞分子生物学研究虽然取得了一定进展,但仍需加强对关键性状和活性成分的全基因组关联分析和调控机制等方面的研究,进而为苦荞生长发育和代谢调控提供科学依据.
Fagopyrum Tataricum is an important medicinal and edible crop with high economic benefits and scientific value.With the rapid development of the modern high-throughput sequencing technology,a convenient and fast way for the study of molecular biology in Fagopyrum Tataricum arises.In this review,we present the advances in molecular biology of Fagopyrum Tataricum,including the molecular markers,genetic diversity,omics,and functional gene.Great achievements have been made in molecular biology research of Fagopyrum Tataricum.It is suggested that more research should be conducted on the genome-wide association analysis and the regulation mechanism of the key traits and the active component in order to provide scientific basis for the growth development and the metabolic regulation in Fagopyrum Tataricum.
Fagopyrum Tataricum is an important medicinal and edible crop with high economic benefits and scientific value.With the rapid development of the modern high-throughput sequencing technology,a convenient and fast way for the study of molecular biology in Fagopyrum Tataricum arises.In this review,we present the advances in molecular biology of Fagopyrum Tataricum,including the molecular markers,genetic diversity,omics,and functional gene.Great achievements have been made in molecular biology research of Fagopyrum Tataricum.It is suggested that more research should be conducted on the genome-wide association analysis and the regulation mechanism of the key traits and the active component in order to provide scientific basis for the growth development and the metabolic regulation in Fagopyrum Tataricum.
引文
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[8]令狐斌,侯思宇,孙朝霞,等.苦荞SRAP分子标记体系优化与遗传多样性分析[J].中国农业大学学报,2015,20(1):37-43.
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[12]黎瑞源,潘凡,陈庆富,等.苦荞转录组EST-SSR发掘及多态性分析[J].中国农业科技导报,2015,17(4):42-52.
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[14]黎瑞源,石桃雄,陈其皎,等.中国35个苦荞审定品种EST-SSR指纹图谱构建与遗传多样性分析[J].植物科学学报,2017,35(2):267-275.
[15]Logacheva M D,Penin A A,Sutormin R A,et al.A draft genome sequence of tartary buckwheat,fagopyrum tataricum[C]//2014 International Plant and Animal Genome Conference XXII.San Diego,CA,USA:PAG,2014.
[16]Zhang L,Li X,Ma B,et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance[J].Molecular Plant,2017,10(9):1224-1237.
[17]Liu M,Zheng T,Ma Z,et al.The complete chloroplast genome sequence of tartary buckwheat cultivar miqiao1(fagopyrum tataricum gaertn)[J].Mitochondrial DNA Part B,2016,1(1):577-578.
[18]Cho K S,Yun B K,Yoon Y H,et al.Completec hloroplast genome sequence of tartary buckwheat(fagopyrum tataricum)and comparative analysis with common buckwheat(F esculentum)[J].PLoS One,2015,10:e0125332.
[19]Maher C A,Kumar-Sinha C,Cao X,et al.Transcriptome sequencing to detect gene fusions in cancer[J].Nature,2009,458(7234):97-101.
[20]Yao H,Li C,Zhao H,et al.Deeps equencing of the transcriptome reveals distinct flavonoid metabolism features of black tartary buckwheat(fagopyrum tataricum garetn)[J].Prog Biophys Mol Biol,2016,124:49.
[21]Zhu H,Wang H,Zhu Y,et al.Genome-wide transcriptomic and phylogenetic analyses reveal distinct aluminum-tolerance mechanisms in the aluminum-accumulating species buckwheat(fagopyrum tataricum)[J].Bmc Plant Biol,2015,15(1):16.
[22]Yao P,Zhao H,Luo X,et al.Fagopyrum tataricum FtWD40,functions as a positive regulator of anthocyanin biosynthesis in transgenic tobacco[J].J Plant Growth Regul,2017,36(3):1-11.
[23]Zhang K,Logacheva M D,Meng Y,et al.Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in fagopyrum tataricum[J].J Exp Bot,2018,69(8):1955-1966.
[24]Zhou M,Sun Z,Ding M,et al.FtSAD2 and FtJAZ1 regulate activity of the FtMYB11 transcription repressor of the phenylpropanoid pathway in fagopyrum tataricum[J].New Phytol,2017,216(3):814-828.
[25]Zhou M,Wang C,Qi L,et al.Ectopic expression of fagopyrum tataricum,FtMYB12,improves cold tolerance in arabidopsis thaliana[J].J Plant Growth Regul,2015,34(2):362-371.
[2]路之娟,张永清,张楚,等.不同基因型苦荞苗期抗旱性综合评价及指标筛选[J].中国农业科学,2017,50(17):3311-3322.
[3]王静波,赵江林,彭镰心,等.苦荞发酵食品研究进展[J].成都大学学报(自然科学版),2013,32(1):9-11.
[4]Zhu F.Chemical composition and health effects of tartary buckwheat[J].Food Chem,2016,203:231-245.
[5]Wang L,Yang X,Qin P,et al.Flavonoid composition,antibacterial and antioxidant properties of tartary buckwheat bran extract[J].Ind Crop Prod,2013,49(8):312-317.
[6]Hu Y,Zhao Y,Ren D,et al.Hypoglycemic and hepatoprotective effects of d-chiro-inositol-enriched tartary buckwheat extract in high fructose-fed mice[J].Food Funct,2015,6(12):3760-3769.
[7]Wu Q,Bai X,Zhao W,et al.De novo assembly and analysis of tartary buckwheat(fagopyrum tataricum garetn)transcriptome discloses key regulators involved in saltstress response[J].Genes,2017,8(10):255.
[8]令狐斌,侯思宇,孙朝霞,等.苦荞SRAP分子标记体系优化与遗传多样性分析[J].中国农业大学学报,2015,20(1):37-43.
[9]高帆,宋饼.基于AFLP标记的苦荞种质资源遗传多样性研究[J].江苏农业科学,2016,45(5):122-126.
[10]胡亚妮,张宗文,吴斌,等.基于ITS和ndhF-rpl32序列的荞麦种间亲缘关系分析[J].生物多样性,2016,24(3):296-303.
[11]Hou S,Sun Z,Linghu B,et al.Genetic diversity of buckwheat cultivars(fagopyrum tartaricum,gaertn)assessed with SSR markers developed from genome survey sequences[J].Plant Mol Bio Rep,2016,34(1):233-241.
[12]黎瑞源,潘凡,陈庆富,等.苦荞转录组EST-SSR发掘及多态性分析[J].中国农业科技导报,2015,17(4):42-52.
[13]屈洋,周瑜,王钊,等.苦荞产区种质资源遗传多样性和遗传结构分析[J].中国农业科学,2016,49(11):2049-2062.
[14]黎瑞源,石桃雄,陈其皎,等.中国35个苦荞审定品种EST-SSR指纹图谱构建与遗传多样性分析[J].植物科学学报,2017,35(2):267-275.
[15]Logacheva M D,Penin A A,Sutormin R A,et al.A draft genome sequence of tartary buckwheat,fagopyrum tataricum[C]//2014 International Plant and Animal Genome Conference XXII.San Diego,CA,USA:PAG,2014.
[16]Zhang L,Li X,Ma B,et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance[J].Molecular Plant,2017,10(9):1224-1237.
[17]Liu M,Zheng T,Ma Z,et al.The complete chloroplast genome sequence of tartary buckwheat cultivar miqiao1(fagopyrum tataricum gaertn)[J].Mitochondrial DNA Part B,2016,1(1):577-578.
[18]Cho K S,Yun B K,Yoon Y H,et al.Completec hloroplast genome sequence of tartary buckwheat(fagopyrum tataricum)and comparative analysis with common buckwheat(F esculentum)[J].PLoS One,2015,10:e0125332.
[19]Maher C A,Kumar-Sinha C,Cao X,et al.Transcriptome sequencing to detect gene fusions in cancer[J].Nature,2009,458(7234):97-101.
[20]Yao H,Li C,Zhao H,et al.Deeps equencing of the transcriptome reveals distinct flavonoid metabolism features of black tartary buckwheat(fagopyrum tataricum garetn)[J].Prog Biophys Mol Biol,2016,124:49.
[21]Zhu H,Wang H,Zhu Y,et al.Genome-wide transcriptomic and phylogenetic analyses reveal distinct aluminum-tolerance mechanisms in the aluminum-accumulating species buckwheat(fagopyrum tataricum)[J].Bmc Plant Biol,2015,15(1):16.
[22]Yao P,Zhao H,Luo X,et al.Fagopyrum tataricum FtWD40,functions as a positive regulator of anthocyanin biosynthesis in transgenic tobacco[J].J Plant Growth Regul,2017,36(3):1-11.
[23]Zhang K,Logacheva M D,Meng Y,et al.Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in fagopyrum tataricum[J].J Exp Bot,2018,69(8):1955-1966.
[24]Zhou M,Sun Z,Ding M,et al.FtSAD2 and FtJAZ1 regulate activity of the FtMYB11 transcription repressor of the phenylpropanoid pathway in fagopyrum tataricum[J].New Phytol,2017,216(3):814-828.
[25]Zhou M,Wang C,Qi L,et al.Ectopic expression of fagopyrum tataricum,FtMYB12,improves cold tolerance in arabidopsis thaliana[J].J Plant Growth Regul,2015,34(2):362-371.