小型猪与大鼠不同记录部位诱发VEMP的比较
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摘要
目的分析正常小型猪不同记录部位(颈部伸肌、咬肌)的前庭诱发肌源性电位(vestibular evoked myogenic potential,VEMP)波形图,并与大鼠的VEMP波形图进行比较;方法各选取12只前庭功能正常的大鼠和小型猪,进行麻醉之后,用自制装置进行固定,1000Hz强短声诱发颈部伸肌、咬肌肌源性电位,并记录其波形;结果大鼠颈部伸肌诱发的肌源性电位,第一个正向波P的潜伏期为6.45±0.23ms,振幅约1.45±0.49uv,80dB SPL引出率为58%;咬肌诱发肌源性电位,第一个正向波P的潜伏期为6.38±0.34ms,振幅约1.57±0.35uv,阈值80dB SPL引出率为50%。小型猪颈部伸肌诱发肌源性电位,第一个正向波P的潜伏期7.65±0.64ms,振幅1.66±0.34uv,80dBSPL的引出率为58%;咬肌诱发肌源性电位,第一个正向波P的潜伏期7.60±0.78ms,振幅1.31±0.28uv,80dBSPL的引出率为50%。结论小型猪和大鼠不同部位记录得到的VEMP,只有振幅和引出率的差异,而潜伏期没有统计学意义;从波形的重复性、波幅的大小、引出率以及潜伏期的综合考虑,小型猪咬肌部位记录的VEMP波形优于颈部伸肌,大鼠颈部伸肌记录得到的VEMP波形优于咬肌;在相同部位记录到VEMP第一个正向P波的潜伏期时间、波幅的大小方面小型猪较大鼠的稍高。
Objective To report vestibular evoked myogenic potentials from different recording sites(neck extensor muscle and masseter muscle) in mini pigs and rats.Methods Potentials were recorded from neck extensor muscles or masseter muscles in normal adult Bama mini pigs and rats anesthetized with 3%pentobarbital sodium and Sumianxin II using1000 Hz tone bursts.Results At 80 dB SPL,the latency,amplitude and response rate of the first positive wave P was 6.45±0.23 ms,1.45±0.49 uv and 58%,respectively,from rat neck extensor muscles;6.38±0.34 ms,1.57±0.35 uv and 50%from rat masseter muscles;7.65±0.64 ms,1.66±0.34 uv and 58%from mini pig neck extensor muscles;and 7.65±0.64 ms,.31±0.28 uv and 50%from mini pig masseter muscles.Conclusion VEMP can be recorded from neck extensor and masseter muscles in mini pigs and rats.Within a given species,VEMP amplitude and response rate are dependent on the site of recording,while latency is not.Analysis of the responses suggests that the optimal VEMP recording site is the masseter muscle in mini pigs but cervical extensor muscles in rats.At the same recording site,the latency and amplitude of VEMP are slightly greater in mini pigs than in rats.
Objective To report vestibular evoked myogenic potentials from different recording sites(neck extensor muscle and masseter muscle) in mini pigs and rats.Methods Potentials were recorded from neck extensor muscles or masseter muscles in normal adult Bama mini pigs and rats anesthetized with 3%pentobarbital sodium and Sumianxin II using1000 Hz tone bursts.Results At 80 dB SPL,the latency,amplitude and response rate of the first positive wave P was 6.45±0.23 ms,1.45±0.49 uv and 58%,respectively,from rat neck extensor muscles;6.38±0.34 ms,1.57±0.35 uv and 50%from rat masseter muscles;7.65±0.64 ms,1.66±0.34 uv and 58%from mini pig neck extensor muscles;and 7.65±0.64 ms,.31±0.28 uv and 50%from mini pig masseter muscles.Conclusion VEMP can be recorded from neck extensor and masseter muscles in mini pigs and rats.Within a given species,VEMP amplitude and response rate are dependent on the site of recording,while latency is not.Analysis of the responses suggests that the optimal VEMP recording site is the masseter muscle in mini pigs but cervical extensor muscles in rats.At the same recording site,the latency and amplitude of VEMP are slightly greater in mini pigs than in rats.
引文
1 K Shimizu,T Murofushi,M Sakurai.Vestibular evoked myogenic potentials inmultiple sclerosis[J].Neurol Neurosurg Psychiatry,2000,69:276—277.
2 Taylor RL,Bradshaw AP,Halmagyi GM,Welgampola MS.Tuning characteristics of ocular and cervical vestibular evoked myogenic potentials in intact and dehiscent ears[J].Audiol Neurootol 2012;17:207-18.
3 Deriu F,Podda MV,Chessa G,et al.Trigeminal integration of vestibular and forelimb nerve inputs[J].Arch Ital Biol.1999,137(1):63-73.
4 Murofushi T.Vestibular evoked myogenic potential[J].World J Otorhinolar-yngol 2014;4:6-11
5谢溯江.博士冲国人民解放军军医进修学院(2007)Dr.Xie Sujiang.Chinese PLA postgraduate medical school(2007).
6任丽丽.博士,中国人民解放军医学院(2013).Dr.Ren Lili,the Chinese people's Liberation Army Medical College(2013).
7 Hsu Q K.Pig model for biomedical research.Taiwan[M].Pig Re search Institute,1982.3—10.
8魏泓.小型猪.医学实验动物学[M],1997,206~211Hong Wei.Pigs.Medical Laboratory Animal Science
9朱星红等版纳微型猪近交系解剖组织学.高等教育出版社[M]。2004.40-46Hongxing Zhu,Bingyin Su,Dajun Ying.Anatohistology of Banna Mini pig lnbred-lines[M].Higher Education Press.2004.40-46
10左艳芳,张天飞,张集建成巴比妥钠在实验小型猪麻醉中的应用[J].药物研究,2002,11(8):33.Yanfang Zuo,Tianfei Zhang.Jijain Zhang.Pentobarbital sodium was applied in experimental miniature pigs anesthesia[J].drug research,2002,11(8):33.
11 Lopez-Escamez JA,Carey J,Chung WH,Goebel JA,Magnusson M.Mandala M,et al.Diagnostic criteria for Meniere's disease[J].J Vestib Res 2015;25:1-7..
12 Taylor RL,Bradshaw AP,Halmagyi GM,Welgampola MS.Tuning characteristics of ocular and cervical vestibular evoked myogenic potentials in intact and dehiscent ears[J].Audiol Neurootol 2012;17:207-18.
13王博琛.颈部前庭诱发肌源性电位的影响因素[J],中华耳科学杂志,2010,10:541-544.Boshen Wang.Influence factors of cervical vestibular evoked potential[J],Chinese Journal of Otology.2010,10:541-544.
14周娜,吴子明,张素珍等.不同刺激模式前庭诱发肌源性电位的反应特性[J].中华耳鼻咽喉科杂志,2004 39:483-485.Na Zhou,Ziming Wu,Suzhen Zhang,et al.Response characteristics of vestibular evoked potentials in different stimulation modes[J].Chinese Journal of Otology.2004 39:483-485.
15 Zhang AS,Govender S,Colebatch JG.Tuning of the ocular vestibular evoked myogenic potential(oVEMP)to air—and bone—conducted sound stimulation in superior canal dehiscence[J].Exp Brain Res2012;223:51-64.
16 Papathanasiou ES,Murofushi T,Akin FW,Colebatch JG.International guide-lines for the clinical application of cervical vestibular evoked myogenic potentials:an expert consensus report[J].Clin Neurophysiol 2014;125:658-66.
17孙伟,王锦玲,邱建华.等.单侧及双侧短纯音刺激前庭诱发肌源性电位观察[J].听力学及言语疾病杂志.2005,13:149-151.Wei Sun,Jingling Wang,Jianhua Qiu,et ai.Vestibular evoked myogenic:potentials of unilateral and bilateral short tone stimulus Observation[J].Journal of Audiology and Speech Pathology.2005,13:149-151.
18 Akin FW,Murnane OD,Panus PC,et al.The influence of voluntary tonic EMG level on the vestibular-evoked myogenic potential[J].J Rehabil Res Dev.2004,41:473-480
19 Westin M,Brantberg K.Mastoid and vertex low—frequency vibration-inducedoVEMP in relation to medially directed acceleration of the labyrinth[J].Clin Neurophysiol 2014;125:615-20.
20王博琛.硕士南方医科大学.2010.Ma.Wang Boshen.Southern Medical University.2010.
21吴子明,张素珍,杨伟炎,等.前庭诱发的肌源性电位原理与应用[J].中华耳科学杂志,2004,2:70-72,47.Ziming Wu.Suzhen Zhang,Weiyan Yang.et ai.Principle and application of vestibular evoked potentials[J].Chinese Journal of Otology.2004,2:70-72,47.
22 Cazals Y,Erre JP,Aurousseau C.Eighth nerve auditory evoked responsesrecorded at the base of the vestibular nucleus in the guinea pig[J].Hear Res.1987 31(1):93-97.
23 Didier A,Cazals Y.Acoustic responses recorded from the saccular bundleon the eighth nerve of the guinea pig[J].Hearg Res.1989 37(2):123-127
2 Taylor RL,Bradshaw AP,Halmagyi GM,Welgampola MS.Tuning characteristics of ocular and cervical vestibular evoked myogenic potentials in intact and dehiscent ears[J].Audiol Neurootol 2012;17:207-18.
3 Deriu F,Podda MV,Chessa G,et al.Trigeminal integration of vestibular and forelimb nerve inputs[J].Arch Ital Biol.1999,137(1):63-73.
4 Murofushi T.Vestibular evoked myogenic potential[J].World J Otorhinolar-yngol 2014;4:6-11
5谢溯江.博士冲国人民解放军军医进修学院(2007)Dr.Xie Sujiang.Chinese PLA postgraduate medical school(2007).
6任丽丽.博士,中国人民解放军医学院(2013).Dr.Ren Lili,the Chinese people's Liberation Army Medical College(2013).
7 Hsu Q K.Pig model for biomedical research.Taiwan[M].Pig Re search Institute,1982.3—10.
8魏泓.小型猪.医学实验动物学[M],1997,206~211Hong Wei.Pigs.Medical Laboratory Animal Science
9朱星红等版纳微型猪近交系解剖组织学.高等教育出版社[M]。2004.40-46Hongxing Zhu,Bingyin Su,Dajun Ying.Anatohistology of Banna Mini pig lnbred-lines[M].Higher Education Press.2004.40-46
10左艳芳,张天飞,张集建成巴比妥钠在实验小型猪麻醉中的应用[J].药物研究,2002,11(8):33.Yanfang Zuo,Tianfei Zhang.Jijain Zhang.Pentobarbital sodium was applied in experimental miniature pigs anesthesia[J].drug research,2002,11(8):33.
11 Lopez-Escamez JA,Carey J,Chung WH,Goebel JA,Magnusson M.Mandala M,et al.Diagnostic criteria for Meniere's disease[J].J Vestib Res 2015;25:1-7..
12 Taylor RL,Bradshaw AP,Halmagyi GM,Welgampola MS.Tuning characteristics of ocular and cervical vestibular evoked myogenic potentials in intact and dehiscent ears[J].Audiol Neurootol 2012;17:207-18.
13王博琛.颈部前庭诱发肌源性电位的影响因素[J],中华耳科学杂志,2010,10:541-544.Boshen Wang.Influence factors of cervical vestibular evoked potential[J],Chinese Journal of Otology.2010,10:541-544.
14周娜,吴子明,张素珍等.不同刺激模式前庭诱发肌源性电位的反应特性[J].中华耳鼻咽喉科杂志,2004 39:483-485.Na Zhou,Ziming Wu,Suzhen Zhang,et al.Response characteristics of vestibular evoked potentials in different stimulation modes[J].Chinese Journal of Otology.2004 39:483-485.
15 Zhang AS,Govender S,Colebatch JG.Tuning of the ocular vestibular evoked myogenic potential(oVEMP)to air—and bone—conducted sound stimulation in superior canal dehiscence[J].Exp Brain Res2012;223:51-64.
16 Papathanasiou ES,Murofushi T,Akin FW,Colebatch JG.International guide-lines for the clinical application of cervical vestibular evoked myogenic potentials:an expert consensus report[J].Clin Neurophysiol 2014;125:658-66.
17孙伟,王锦玲,邱建华.等.单侧及双侧短纯音刺激前庭诱发肌源性电位观察[J].听力学及言语疾病杂志.2005,13:149-151.Wei Sun,Jingling Wang,Jianhua Qiu,et ai.Vestibular evoked myogenic:potentials of unilateral and bilateral short tone stimulus Observation[J].Journal of Audiology and Speech Pathology.2005,13:149-151.
18 Akin FW,Murnane OD,Panus PC,et al.The influence of voluntary tonic EMG level on the vestibular-evoked myogenic potential[J].J Rehabil Res Dev.2004,41:473-480
19 Westin M,Brantberg K.Mastoid and vertex low—frequency vibration-inducedoVEMP in relation to medially directed acceleration of the labyrinth[J].Clin Neurophysiol 2014;125:615-20.
20王博琛.硕士南方医科大学.2010.Ma.Wang Boshen.Southern Medical University.2010.
21吴子明,张素珍,杨伟炎,等.前庭诱发的肌源性电位原理与应用[J].中华耳科学杂志,2004,2:70-72,47.Ziming Wu.Suzhen Zhang,Weiyan Yang.et ai.Principle and application of vestibular evoked potentials[J].Chinese Journal of Otology.2004,2:70-72,47.
22 Cazals Y,Erre JP,Aurousseau C.Eighth nerve auditory evoked responsesrecorded at the base of the vestibular nucleus in the guinea pig[J].Hear Res.1987 31(1):93-97.
23 Didier A,Cazals Y.Acoustic responses recorded from the saccular bundleon the eighth nerve of the guinea pig[J].Hearg Res.1989 37(2):123-127