小型猪肌源性诱发前庭电位的检测
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摘要
目的探索小型猪肌源性诱发前庭电位(Vestibular-evoked myogenic potential,VEMP)的最佳检测方法。方法选取正常成年的雌性小型巴马香猪,以3%戊巴比妥钠+速眠新Ⅱ进行麻醉之后,用自制装置进行固定,1000Hz强短声诱发颈部伸肌肌源性电位和咬肌肌源性电位,并记录其波形;结果颈部伸肌诱发肌源性电位,第一个正向波P的潜伏期7.65±0.64ms,振幅1.66±0.34uv,80dBSPL的引出率为75%;咬肌诱发肌源性电位,第一个正向波P的潜伏期7.60±0.78ms,振幅1.31±0.28uv,80dBSPL的引出率为66%。结论小型猪颈部伸肌和咬肌在强声下诱发的肌源性电位潜伏期和阈值均一致;颈部伸肌部位所记录到的VEMF波幅稍高于咬肌部位记录到波幅;但相比之下,咬肌位置表浅,便于定位,肌肉组织发达,肌紧张性强,更易于肌源性诱发电位的记录。
Objective To report detection of vestibular-evoked myogenic potentials(VEMP) in the miniature pig.Methods Potentials evoked by 1000 Hz tone bursts were recorded from neck extensor muscles and the masseter muscles in normal adult Bama miniature pigs anesthetized with 3%pentobarbital sodium and Sumianxin Ⅱ.Results The latency of the first positive wave P from neck extensor muscles was 7.65±0.64 ms,with an amplitude of 1.66±0.34 uv and a rate of successful induction of 75%at 80 dB SPL.The latency of potentials evoked from the masseter muscles was 7.60±0.78 ms,with an amplitude of 1.31 ±0.28 uv and a rate successful induction of 66%at 80 dB SPL.Conclusion The latencies and thresholds of VEMPs recorded from neck extensor muscles and the masseter muscles appear to be comparable in normal adult Bama miniature pigs,although the amplitude recorded from neck extensor muscles seems to be higher than that from the masseter muscles.However,because of their usually relatively superficial and easily accessible location,as well as their large volume and strong contractions,masseter muscles may be better target muscles for recording myogenic potentials.
Objective To report detection of vestibular-evoked myogenic potentials(VEMP) in the miniature pig.Methods Potentials evoked by 1000 Hz tone bursts were recorded from neck extensor muscles and the masseter muscles in normal adult Bama miniature pigs anesthetized with 3%pentobarbital sodium and Sumianxin Ⅱ.Results The latency of the first positive wave P from neck extensor muscles was 7.65±0.64 ms,with an amplitude of 1.66±0.34 uv and a rate of successful induction of 75%at 80 dB SPL.The latency of potentials evoked from the masseter muscles was 7.60±0.78 ms,with an amplitude of 1.31 ±0.28 uv and a rate successful induction of 66%at 80 dB SPL.Conclusion The latencies and thresholds of VEMPs recorded from neck extensor muscles and the masseter muscles appear to be comparable in normal adult Bama miniature pigs,although the amplitude recorded from neck extensor muscles seems to be higher than that from the masseter muscles.However,because of their usually relatively superficial and easily accessible location,as well as their large volume and strong contractions,masseter muscles may be better target muscles for recording myogenic potentials.
引文
1 K Shimizu,T Murofushi,M Sakurai.Vestibular evoked myogenic potentials in multiple sclerosis[J].Neurol Neurosurg Psychiatry,2000,69:276-277.
2 Weiwei,G.,Haijin,Y.,Lili,R.,Lei,C,Lidong,Z.,Wei,S.,and Shi-Ming,Y.(2015)The Morphology and Electrophysiology of the Cochlea of the Miniature Pig[J].Anatomical Record 298(3),494-500
3 Institute of Laboratory Animal Resources,Commission on Life Sciences,National Research Council,Guide for the Care and Use of Laboratory Animals[M],National Academy Press,Washington D.C.1996.
4 Guo,W.-W.,Yi,H.,Zhang,Y.,Ren,L.-L.,Chen,L.,Zhao,L.D.,Yu,N.,He,D.,and Yang,S.-M.(2015)The morphological and functional development of the stria vascularis in miniature pigs.Reproduction,Fertility and Development
5 Ting-Hua Yang,Yi-Ho Young,Click-evoked myogenic potentials recorded on alert guinea pigs[J],Hearing Research,2005,(205)277-283
6 Koichi Sakakura,Motoaki Miyashita,Kazuaki Chikamatsu,Katsumasa Takahashi,Tone burst-evoked myogenic potentials in rat neck extensor and flexor muscles[J],Nobuhiko Furuya Hearing Research2003,(185)57-64Cliff A.
7 Megerian,and Qing Y.Zheng.Vestibular Evoked Myogenic Potentials in Normal Mice and Phex Mice With Spontaneous Endolymphatic Hydrops Kianoush Sheykholeslami[J],Otol Neurotol.2009 June;30(4):535-544.
8朱星红等版纳微型猪近交系解剖组织学[M],.高等教育出版社。2004.40-46.Hongxing Zhu,Bingyin Su,Dajun Ying.Anatohistology of Banna Minipig Inbred-lines[M],.Higher Education Press.2004.40-46.
9孙伟.博士,华中科技大学同济医学院(2010)Dr.Wei Sun,Tongji Medical College of Huazhong University of Science and Technology(2010)
10谢溯江.博士.中国人民解放军军医进修学院(2007).Dr.Xie Sujiang.Chinese PLA postgraduate medical school(2007).
11 Deriu F,Tolu E.Rothwell JC.A short latency vestibulomasseteric ref lex evoked by electrical stimulation over the mastoid in healthy hu mans[J].J Physiol,2003,553(1)267-279.
12 Deriu F,Tolu E,Rothwell JC.A sound-evoked vestibulomasseteric reflex in healthy humans[]].J Neurophysiol,2005,93(5):2739-2751.
13 Colebatch,J.G.and G.M.Halmagyi.Vestibular evoked myogenic potentials in humans[J].Acta Otolaryngol.2000 Jan;120(1):l 12
14尚建勋.动物化学保定药的发展概况[J].中国兽医科技,1992,22(1):18-21Jianxun Shang.An overview of the development of animal chemical stabilization drugs[J].Chinese Journal of Veterinary Science and Technology.1992,22(1):18-21
15刘秋菊,董为人,王元占,等.实验动物麻醉期的呼吸障碍及处理[J].实验动物科学与管理,1995,12(3):44.Qiuju Liu,Weiren Dong.Respiratory disturbance and its treatment during the period of anesthesia in laboratory animals[J].Laboratory Animal Science,1995,12(3):44.
16、叶惠贞,李斯明,陆瑞意.戊巴比妥钠与利多卡因在实验兔麻醉中的应用[J].广州医药,2000,31(1):50.Huizhen Ye,Siming Li.Ruiyi Lu.Pentobarbital Sodium and Lido caine hydrochloride in experimental rabbits Application[J].Guangzhou Medical Journal,2000,31(1):50.
17 Matthews PB.Observations on the automatic compensation of reflex gainon varying the pre-existing level of motor discharge in man.[J].J Physiol.1986 May;374:73-90.374:73-90.
18谢溯江,杨伟炎,贾宏博等.健康人强短声诱发的短潜伏期咬肌肌源性电位[J].中华航空航天医学杂志.2006,18(4):307-313Sujiang Xie.Weiyan Yang,Hongbo Jia,et al.Strong short sound of healthy people of loud click-evoked short latency masseter myogenic potential[J].Chinese Journal of Aerospace Medicine,2006,18(4):307-313
19 Sakakura K,Miyashita M,Chikamatsu K,et al.Tone burst-evoked myogenic potentials in rat neck extensor and flexor muscles[J].Hear Res.2003,185(1-2):57-64.
20 Ferber-Viart,C,R.Duclaux,B.Coll and C.Dubreuil.Myogenic vestibular-evoked potentials in normal subjects:a comparison between responses obtained from sternomastoid and trapezius muscles[J].Acta Otolaryngol,1997;117(4):472-81.2004.40-46.
2 Weiwei,G.,Haijin,Y.,Lili,R.,Lei,C,Lidong,Z.,Wei,S.,and Shi-Ming,Y.(2015)The Morphology and Electrophysiology of the Cochlea of the Miniature Pig[J].Anatomical Record 298(3),494-500
3 Institute of Laboratory Animal Resources,Commission on Life Sciences,National Research Council,Guide for the Care and Use of Laboratory Animals[M],National Academy Press,Washington D.C.1996.
4 Guo,W.-W.,Yi,H.,Zhang,Y.,Ren,L.-L.,Chen,L.,Zhao,L.D.,Yu,N.,He,D.,and Yang,S.-M.(2015)The morphological and functional development of the stria vascularis in miniature pigs.Reproduction,Fertility and Development
5 Ting-Hua Yang,Yi-Ho Young,Click-evoked myogenic potentials recorded on alert guinea pigs[J],Hearing Research,2005,(205)277-283
6 Koichi Sakakura,Motoaki Miyashita,Kazuaki Chikamatsu,Katsumasa Takahashi,Tone burst-evoked myogenic potentials in rat neck extensor and flexor muscles[J],Nobuhiko Furuya Hearing Research2003,(185)57-64Cliff A.
7 Megerian,and Qing Y.Zheng.Vestibular Evoked Myogenic Potentials in Normal Mice and Phex Mice With Spontaneous Endolymphatic Hydrops Kianoush Sheykholeslami[J],Otol Neurotol.2009 June;30(4):535-544.
8朱星红等版纳微型猪近交系解剖组织学[M],.高等教育出版社。2004.40-46.Hongxing Zhu,Bingyin Su,Dajun Ying.Anatohistology of Banna Minipig Inbred-lines[M],.Higher Education Press.2004.40-46.
9孙伟.博士,华中科技大学同济医学院(2010)Dr.Wei Sun,Tongji Medical College of Huazhong University of Science and Technology(2010)
10谢溯江.博士.中国人民解放军军医进修学院(2007).Dr.Xie Sujiang.Chinese PLA postgraduate medical school(2007).
11 Deriu F,Tolu E.Rothwell JC.A short latency vestibulomasseteric ref lex evoked by electrical stimulation over the mastoid in healthy hu mans[J].J Physiol,2003,553(1)267-279.
12 Deriu F,Tolu E,Rothwell JC.A sound-evoked vestibulomasseteric reflex in healthy humans[]].J Neurophysiol,2005,93(5):2739-2751.
13 Colebatch,J.G.and G.M.Halmagyi.Vestibular evoked myogenic potentials in humans[J].Acta Otolaryngol.2000 Jan;120(1):l 12
14尚建勋.动物化学保定药的发展概况[J].中国兽医科技,1992,22(1):18-21Jianxun Shang.An overview of the development of animal chemical stabilization drugs[J].Chinese Journal of Veterinary Science and Technology.1992,22(1):18-21
15刘秋菊,董为人,王元占,等.实验动物麻醉期的呼吸障碍及处理[J].实验动物科学与管理,1995,12(3):44.Qiuju Liu,Weiren Dong.Respiratory disturbance and its treatment during the period of anesthesia in laboratory animals[J].Laboratory Animal Science,1995,12(3):44.
16、叶惠贞,李斯明,陆瑞意.戊巴比妥钠与利多卡因在实验兔麻醉中的应用[J].广州医药,2000,31(1):50.Huizhen Ye,Siming Li.Ruiyi Lu.Pentobarbital Sodium and Lido caine hydrochloride in experimental rabbits Application[J].Guangzhou Medical Journal,2000,31(1):50.
17 Matthews PB.Observations on the automatic compensation of reflex gainon varying the pre-existing level of motor discharge in man.[J].J Physiol.1986 May;374:73-90.374:73-90.
18谢溯江,杨伟炎,贾宏博等.健康人强短声诱发的短潜伏期咬肌肌源性电位[J].中华航空航天医学杂志.2006,18(4):307-313Sujiang Xie.Weiyan Yang,Hongbo Jia,et al.Strong short sound of healthy people of loud click-evoked short latency masseter myogenic potential[J].Chinese Journal of Aerospace Medicine,2006,18(4):307-313
19 Sakakura K,Miyashita M,Chikamatsu K,et al.Tone burst-evoked myogenic potentials in rat neck extensor and flexor muscles[J].Hear Res.2003,185(1-2):57-64.
20 Ferber-Viart,C,R.Duclaux,B.Coll and C.Dubreuil.Myogenic vestibular-evoked potentials in normal subjects:a comparison between responses obtained from sternomastoid and trapezius muscles[J].Acta Otolaryngol,1997;117(4):472-81.2004.40-46.