Toll样受体、前列腺素受体EP2在溃疡性结肠炎组织的表达与炎症细胞因子的关系
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摘要
目的探讨溃疡性结肠炎(UC)组织中Toll样受体、前列腺素受体EP2的表达变化及与组织中炎性细胞因子表达的关系。方法选取我院2016年1月至2017年1月收治的100例UC组织(UC组)、50例正常结肠黏膜组织(对照组),采用免疫组化染色检测两组标本中的Toll样受体2(TLR2)、Toll样受体4(TLR4)、前列腺素受体EP2蛋白的表达水平,采用酶联免疫吸附法(ELISA)检测两组标本中的白细胞介素-17(IL-17)、白细胞介素-10(IL-10),并分析TLR2、TLR4、前列腺素受体EP2与IL-17、IL-10的关系。结果 UC组中TLR2、TLR4、前列腺素受体EP2蛋白阳性表达率59. 00%、61. 00%、69. 00%均高于对照组的26. 00%、18. 00%、26. 00%,差异有统计学意义(P<0. 05);活动期、Ⅲ级UC组中TLR2、TLR4、前列腺素受体EP2蛋白阳性表达均显著的高于非活动期、Ⅰ级+Ⅱ级的UC患者,差异有统计学意义(P <0. 05); TLR2、TLR4、前列腺素受体EP2蛋白阳性的UC组织中的IL-17水平高于阴性表达的患者,IL-10表达水平则低于阴性表达的UC组织,差异有统计学意义(P<0. 05)。结论 Toll样受体、前列腺素受体EP2在UC组织表达上调,并且与UC病情进展及炎症反应程度加重有关。
Objective To investigate the expression of Toll-like receptor and prostaglandin receptor EP2 in ulcerative colitis( UC) and their relationship with the expression of inflammatory cytokines in tissues. Methods 100 cases of UC tissue( UC group) and 50 cases of normal colonic mucosa( control group),from January 2016 to January 2017 in our hospital,were concluded.Toll-like receptor 2( TLR2),Toll-like receptor 4( TLR4) and prostaglandin receptor EP2 protein expression levels were detected by immunohistochemical staining,the interleukin-17( IL-17),interleukin-10( IL-10) in two groupwere detected by enzyme-linked immunosorbent assay( ELISA),and the relationship between TLR2,TLR4,prostaglandin receptor EP2 and IL-17,IL-10 were analyzed.Results The positive expression rates of TLR2,TLR4 and prostaglandin receptor EP2 protein in UC group were 59. 00%,61. 00%and 69. 00%,and they were higher than 26. 00%,18. 00% and 26. 00% in the control group,statistically significant( P<0. 05).The positive expressions of TLR2,TLR4 and prostaglandin receptor EP2 in the active period and grade III UC groups were significantly higher than those in the inactive period and grade I+II UC patients,statistically significant( P<0. 05).The level of IL-17 in UC tissues with TLR2,TLR4 and prostaglandin receptor EP2 protein positive expressions was higher than that in patients with negative expression,and the expression level of IL-10 was lower than that of UC tissues with negative expression,statistically significant( P<0. 05).Conclusion The expression of Toll-like receptor and prostaglandin receptor EP2 is up-regulated in UC tissues,and is associated with the progression of UC and the severity of inflammatory response.
Objective To investigate the expression of Toll-like receptor and prostaglandin receptor EP2 in ulcerative colitis( UC) and their relationship with the expression of inflammatory cytokines in tissues. Methods 100 cases of UC tissue( UC group) and 50 cases of normal colonic mucosa( control group),from January 2016 to January 2017 in our hospital,were concluded.Toll-like receptor 2( TLR2),Toll-like receptor 4( TLR4) and prostaglandin receptor EP2 protein expression levels were detected by immunohistochemical staining,the interleukin-17( IL-17),interleukin-10( IL-10) in two groupwere detected by enzyme-linked immunosorbent assay( ELISA),and the relationship between TLR2,TLR4,prostaglandin receptor EP2 and IL-17,IL-10 were analyzed.Results The positive expression rates of TLR2,TLR4 and prostaglandin receptor EP2 protein in UC group were 59. 00%,61. 00%and 69. 00%,and they were higher than 26. 00%,18. 00% and 26. 00% in the control group,statistically significant( P<0. 05).The positive expressions of TLR2,TLR4 and prostaglandin receptor EP2 in the active period and grade III UC groups were significantly higher than those in the inactive period and grade I+II UC patients,statistically significant( P<0. 05).The level of IL-17 in UC tissues with TLR2,TLR4 and prostaglandin receptor EP2 protein positive expressions was higher than that in patients with negative expression,and the expression level of IL-10 was lower than that of UC tissues with negative expression,statistically significant( P<0. 05).Conclusion The expression of Toll-like receptor and prostaglandin receptor EP2 is up-regulated in UC tissues,and is associated with the progression of UC and the severity of inflammatory response.
引文
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[9]高鸿亮,周禾,姚萍.维生素D_3对急性溃疡性结肠炎大鼠肠黏膜Toll样受体2和髓样分化蛋白2及CD14表达的影响[J].中华实用诊断与治疗杂志,2017,31(07):649-652.
[10]黄萃园,张洪,白瑞丹.Toll样受体4(896A/G)基因多态性与溃疡性结肠炎易感性的Meta分析[J].安徽医药,2018,22(01):63-67.
[11]尹群,薛育政.PGT在溃疡性结肠炎中的表达及其与疾病活动的关系[J].南通大学学报(医学版),2017,37(03):219-220.
[12]刘翔,丁元伟,林虹.溃疡性结肠炎患者肠道不同病变部位Toll样受体4的表达[J].中国现代医学杂志,2015,25(18):38-40.
[13]杨万才,韩安家.慢性结肠炎发生及癌变的分子机制研究进展[J].中华病理学杂志,2016,36(3):145-148.
[14]王倩,田慧,刘靓靓,等.溃疡性结肠炎模型的构建及其炎症反应机制研究[J].神经药理学报,2014,4(05):15-23.
[15]宋军民,杨超,李震,等.结肠癌临床病理特征与Toll样受体4表达的关系[J].中华实验外科杂志,2015,32(7):1686-1688.
[2]Moreno-Rincón E,Benítez JM,Serrano-Ruiz FJ,et al.Prognosis of Patients with Ulcerative Colitis in Sustained Remission After ThiopurinesWithdrawal[J].Inflammatory Bowel Diseases,2015,21(7):1564-1571.
[3]孙宏飞,郭莲怡.Toll样受体在溃疡性结肠炎患者外周血中的表达及其意义[J].解放军医学院学报,2016,26(06):634-640.
[4]尹群,薛育政.尿PGE_2在溃疡性结肠炎中的表达及临床意义[J].江苏医药,2017,43(12):852-854.
[5]孙健,高文艳,林一帆.溃疡性结肠炎病因和发病机制研究进展[J].辽宁中医药大学学报,2017,19(04):94-97.
[6]Miyoshi J,Matsuoka K,Inoue N,et al.Mucosal healing with oral tacrolimus is associated with favorable medium-and long-term prognosis in steroid-refractory/dependent ulcerative colitis patients[J].Journal of Crohn's and Colitis,2013,7(12):609-614.
[7]Nakarai A.Prognosis of ulcerative colitis differs between patients with complete and partial mucosal healing,which can be predicted from the platelet count[J].World Journal of Gastroenterology,2014,20(48):367-369.
[8]Takayama T,Okamoto S,Hisamatsu T,et al.Computer-Aided Prediction of Long-Term Prognosis of Patients with Ulcerative Colitis after Cytoapheresis Therapy[J].PLOS ONE,2015,10(6):197-199.
[9]高鸿亮,周禾,姚萍.维生素D_3对急性溃疡性结肠炎大鼠肠黏膜Toll样受体2和髓样分化蛋白2及CD14表达的影响[J].中华实用诊断与治疗杂志,2017,31(07):649-652.
[10]黄萃园,张洪,白瑞丹.Toll样受体4(896A/G)基因多态性与溃疡性结肠炎易感性的Meta分析[J].安徽医药,2018,22(01):63-67.
[11]尹群,薛育政.PGT在溃疡性结肠炎中的表达及其与疾病活动的关系[J].南通大学学报(医学版),2017,37(03):219-220.
[12]刘翔,丁元伟,林虹.溃疡性结肠炎患者肠道不同病变部位Toll样受体4的表达[J].中国现代医学杂志,2015,25(18):38-40.
[13]杨万才,韩安家.慢性结肠炎发生及癌变的分子机制研究进展[J].中华病理学杂志,2016,36(3):145-148.
[14]王倩,田慧,刘靓靓,等.溃疡性结肠炎模型的构建及其炎症反应机制研究[J].神经药理学报,2014,4(05):15-23.
[15]宋军民,杨超,李震,等.结肠癌临床病理特征与Toll样受体4表达的关系[J].中华实验外科杂志,2015,32(7):1686-1688.