蛹虫草主要成分虫草素、腺苷提取方法对比
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摘要
利用前期加速溶剂萃取法提取腺苷、虫草素的优化结果,分别挑选最优处理进行试验,即提取腺苷最适条件为温度70℃、时间10 min、乙醇含量20%、循环2次,提取虫草素最适条件为温度100℃、时间15 min、乙醇含量0%、循环2次。并将该方法与水热回流法、醇热回流法、超声水提法、超声醇提法4种传统方法进行比较,结果表明,加速溶剂萃取法明显优于其他传统4种方法,各个方法之间差异达显著水平(P<0.05);加速溶剂萃取法提取腺苷效果最好,提取量为2 118.07μg/g,提取效果最差的是超声波醇提法,提取量仅有180.72μg/g;加速溶剂萃取法提取的虫草素量为19 678.70μg/g,超声波醇提法提取虫草素效果最差,提取量仅有4 108.27μg/g;加速溶剂萃取法有其明显的优势,操作简单、方便快捷。
引文
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[22] MURAT GIZIR A,NUZHET TURKER,ERDEM ARTUVAN.Pressurized acidifide water extraction of black carrot(Daucus carota ssp.saticus car.atrorubens Alef.)anthocyanins[J].European Food Research and Technology,2008,226:363-370.
[23]陈方圆,马麦燕,张相锋,等.加速溶剂萃取法提取蛹虫草主要成分工艺优化分析[J].新疆农业科学,2018,55(5):928-935.
[24]韩建华,杨淑芳,孙继红,等.蛹虫草中虫草素、腺苷综合提取工艺研究[J].北方蚕业,2011,32(4):24-27.
[25]申进文,孙晓萍,赵旭,等.人工蛹虫草核苷类成分超声提取工艺优化及HPLC定量分析[J].河南农业大学学报,2011,45(4):391-394.
[26] CONNOLLY G P,DULEY J A.Uridine and its nucleotides:biological actions,therapeutic potentials[J].Trends in Pharmacological Sciences,1999,20(5):218-225.
[27]赵恒强,陈军辉,郭秀春,等.加速溶剂萃取法快速提取黄连中的生物碱[J].分析试验室,2008,27(11):5-9.
[28]石矛,陈丽娜.快速溶剂萃取仪提取保健食品中人参皂苷条件优化[J].食品安全质量检测学报,2016,7(1):39-43.
[2]陈毓荃.生物化学实验方法和技术[M].北京:科学出版社,2003:102-106.
[3] PELLEG A,PORTER R S.The pharmacology of adenosine[J].Pharmacotherapy:The Journal of Human Pharmacology and Drug Therapy,1990,10(3):157-174.
[4] KITAKAZE M,HORI M.Adenosine therapy:a new approach to chronic heart failure[J].Expert Opinon on Investigational Drugs,2009,9(11):2519-2535.
[5]黄子琪,贲松彬,桑育黎,等.微波法与超声破碎法提取蛹虫草菌丝体中虫草素的比较研究[J].食品科学,2009,30(18):176-180.
[6]吴勇,陈卫东,王利,等.蛹虫草培养基中虫草素的几种提取方法比较[J].安徽药业,2010,14(3):284-285.
[7]钟艳梅,黄志全,温凯.人工蛹虫草固体培养残基中虫草素的提取分离研究[J].现代食品科技,2007,23(2):40-42.
[8]王英娟,李多伟,王义潮,等.蛹虫草中虫草素、虫草多糖综合提取工艺研究[J].西北植物学报,2005,25(9):1863-1867.
[9]谭琪明,何珺,文庭池,等.蛹虫草小麦培养基中虫草素提取工艺的研究[J].山地农业生物学报,2011,30(4):357-361.
[10]张嘉,李多伟,任静.正交试验提取虫草素的工艺研究[J].中国新医药,2004,3(2):34-35.
[11] RICHTER B E,JONES B A,PORTER L N,et al.Accelerated solvent extraction:A technique for sample preparation[J].Analytical and Bioanalytical Chemistry,1996,68(6):1033.
[12]王淑红,熊英,王祥红.加速溶剂萃取法用于灯盏细辛中灯盏乙素的测定[J].药物分析杂志,2006,26(5):703.
[13]陈舰,张景华.快速溶剂萃取-高效液相法测定茶叶中多菌灵残留量[J].广东化工,2011,38(1):170.
[14]刘斌.加压溶剂提取-HPLC法同时测定老鹰茶中7个黄酮类化合物[J].药物分析杂志,2010,30(8):1424.
[15] WONG L J,CURTIS L W.R.ecent advances in extraction of neutraceuticals from plants[J].Trends in Food Science&Technology,2006,17(6):300.
[16]刘志,阮长春,刘天志,等.HPLC法同时测定林下参、鲜人参、生晒参和红参中14种人参皂苷[J].中草药,2012,43(12):2431-2434.
[17]喻凌寒,宋之光,陈江韩,等.快速溶剂萃取反向高效液相色谱法测定青蒿中的青蒿素[J].分析实验室,2006(8):68-71.
[18]刘平怀,刘洋洋,时杰,等.加速溶剂萃取(ASE)技术提取海南萝芙木活性成分[J].精细化工,2009,26(11):1120-1123.
[19] PAPAEFSTATHIOU G,POLYCHRONOPOULOS P,ALIGIANNIS N,et al.Comparaive study of accelerated solvent extraction and supercritical fluid extraction of total phenolics and flavonoids from Sideritis faeseri subsp attica and atudy pf antioxidant activity[J].Panta Medica,2008,74(9):1122.
[20]宋文斌,代英成,许敏,等.加速溶剂萃取-液相色谱-紫外检测法测定人参中多种人参皂甙含量[J].现代科学仪器,2009(6):104-108.
[21] ARAPITSAS P,SJOBERG P J R,TURNER C.Characterisation of anthocyanins in red cabbage using high resolution liquid chromatography coupled with photodeode array detection and electrospray ionizationliner ion trap mass spectrometry[J].Food Chemistry,2008,109:219-226.
[22] MURAT GIZIR A,NUZHET TURKER,ERDEM ARTUVAN.Pressurized acidifide water extraction of black carrot(Daucus carota ssp.saticus car.atrorubens Alef.)anthocyanins[J].European Food Research and Technology,2008,226:363-370.
[23]陈方圆,马麦燕,张相锋,等.加速溶剂萃取法提取蛹虫草主要成分工艺优化分析[J].新疆农业科学,2018,55(5):928-935.
[24]韩建华,杨淑芳,孙继红,等.蛹虫草中虫草素、腺苷综合提取工艺研究[J].北方蚕业,2011,32(4):24-27.
[25]申进文,孙晓萍,赵旭,等.人工蛹虫草核苷类成分超声提取工艺优化及HPLC定量分析[J].河南农业大学学报,2011,45(4):391-394.
[26] CONNOLLY G P,DULEY J A.Uridine and its nucleotides:biological actions,therapeutic potentials[J].Trends in Pharmacological Sciences,1999,20(5):218-225.
[27]赵恒强,陈军辉,郭秀春,等.加速溶剂萃取法快速提取黄连中的生物碱[J].分析试验室,2008,27(11):5-9.
[28]石矛,陈丽娜.快速溶剂萃取仪提取保健食品中人参皂苷条件优化[J].食品安全质量检测学报,2016,7(1):39-43.