餐厨垃圾废液制备高效解磷菌肥
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摘要
从小麦土中筛选出2株高效解磷菌P1和P7,其解磷能力在10 d内达到117.57 mg·L~(-1)和219.59 mg·L~(-1)。经16S rDNA测序鉴定及构建系统进化树,鉴定P1为Enterobacter cloacae,P7为Kosakonia cowanii。通过正交实验及单因素实验,对解磷菌在餐厨废液中生长条件进行了优化。结果表明,Kosakonia cowanii生长条件影响程度的顺序依次为温度>pH>摇床转速>接种量,最优的培养条件为温度25℃,pH=7,摇床转速140 r·min~(-1),接种量1.0%。将筛选的解磷菌制备成解磷菌肥,并进行大蒜盆栽实验。结果表明,30 d后,土壤有效磷相比空白提升了16.32%,大蒜叶宽与叶数相比空白分别提升了63.16%和60.00%。
Two highly-effective phosphate-solubilizing bacteria, named P1 and P7, were screened from wheat soil, and their phosphate-solubilizing abilities within 10 d reached 117.57 mg · L~(-1) and 219.59 mg · L~(-1),respectively. On the basis of 16 S rDNA sequencing and phylogenetic tree construction, P1 and P7 were identified as Enterobacter cloacae and Kosakonia cowanii, respectively. The orthogonal and single factor experiments were carried out to optimize the growth conditions of phosphate-solubilizing bacteria in the food waste-recycling wastewater. The results showed that the significance of factors affecting the growth conditions of Kosakonia cowanii bacteria was temperature>pH>shaking speed>inoculum concentration. The optimal culture conditions determined by orthogonal experiment were following: temperature of 25 ℃, pH of 7, shaking speed of140 r · min~(-1), and inoculum concentration of 1.0%. The phosphate-solubilizing bacteria fertilizer was prepared from the screened phosphate-solubilizing bacteria, and the pot experiments of cultivating garlic were conducted accordingly. The results showed that the available phosphorus in soil increased by 16.32% compared to control check after 30 days, and the width and number of garlic leaf increased by 63.16% and 60.00%, respectively.
Two highly-effective phosphate-solubilizing bacteria, named P1 and P7, were screened from wheat soil, and their phosphate-solubilizing abilities within 10 d reached 117.57 mg · L~(-1) and 219.59 mg · L~(-1),respectively. On the basis of 16 S rDNA sequencing and phylogenetic tree construction, P1 and P7 were identified as Enterobacter cloacae and Kosakonia cowanii, respectively. The orthogonal and single factor experiments were carried out to optimize the growth conditions of phosphate-solubilizing bacteria in the food waste-recycling wastewater. The results showed that the significance of factors affecting the growth conditions of Kosakonia cowanii bacteria was temperature>pH>shaking speed>inoculum concentration. The optimal culture conditions determined by orthogonal experiment were following: temperature of 25 ℃, pH of 7, shaking speed of140 r · min~(-1), and inoculum concentration of 1.0%. The phosphate-solubilizing bacteria fertilizer was prepared from the screened phosphate-solubilizing bacteria, and the pot experiments of cultivating garlic were conducted accordingly. The results showed that the available phosphorus in soil increased by 16.32% compared to control check after 30 days, and the width and number of garlic leaf increased by 63.16% and 60.00%, respectively.
引文
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[17]李媛媛.基于全基因组序列系统分类Kosakonia属的细菌[D].杭州:浙江大学,2016.
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[19]关怡,尹娣,杜茜,等.产纤维素酶菌株Trametes sp. LYW-1的分离鉴定及产酶条件分析[J].中国农业大学学报,2018,23(10):96-102.
[20]李铉军,张先,付长雪,等.利用大酱中的枯草芽孢杆菌提高人参中Rg3含量的研究[J].吉林农业,2018, 30(18):50-53.
[21]蒋欣梅,夏秀华,于锡宏,等.微生物解磷菌肥对大棚茄子生长及土壤有效磷利用的影响[J].浙江大学学报(理学版),2012, 39(6):685-688.
[22]LIU F P, LIU H Q, ZHOU H L, et al. Isolation and characterization of phosphate-solubilizing bacteria from betel nut(Areca catechu)and their effects on plant growth and phosphorus mobilization in tropical soils[J]. Biology and Fertility of Soils,2014, 50(6):927-937.
[23]任友花,王羿超,李娜,等.微生物肥料高效解磷菌筛选及解磷机理探究[J].江苏农业科学,2016, 44(12):537-540.
[24]RAJWAR J, CHANDRA R, SUYAL D C, et al. Comparative phosphate solubilizing efficiency of psychrotolerant Pseudomonas jesenii MP1 and Acinetobacter sp ST02 against chickpea for sustainable hill agriculture[J]. Biologia, 2018, 73(8):793-802.
[25]邢芳芳,徐文凤,宋涛,等.一株高效溶磷真菌的溶磷特性及其促生效果研究[J].华北农学报,2013, 28(S1):357-360.
[26]王莉晶,高晓蓉,孙嘉怡,等.土壤解磷微生物作用机理及解磷菌肥对作物生长的影响[J].安徽农业科学,2008, 36(14):5948-5950.
[27]章爱群,郭海如,崔雪梅,等.主成分分析磷、砷交互作用对水稻生长的影响[J].环境科学与技术,2017, 40(7):48-53.
[28]林英,司春灿,韩文华,等.解磷微生物研究进展[J].江西农业学报,2017, 29(2):99-403.
[29]FERNANDO W G D, NAKKEERAN S, ZHANG Y. PGPR:Biocontrol and Biofertilization[M]. Berlin:Springer Netherlands,2005:67-109.
[30]刘泽平,王志刚,徐伟慧,等.水稻根际促生菌的筛选鉴定及促生能力分析[J].农业资源与环境学报,2018, 35(2):119-125.
[2]张玉静,蒋建国,王佳明.pH值对餐厨垃圾厌氧发酵产挥发性脂肪酸的影响[J].中国环境科学,2013, 33(4):680-684.
[3]王罕,戴庆武,顾礼炜,等.餐厨垃圾废水综合处理技术进展[J].广东化工, 2013, 40(17):155-156.
[4]任连海,聂永丰,刘建国,等.餐厨垃圾湿热处理的影响因素[J].清华大学学报(自然科学版),2006, 46(9):1551-1554.
[5]赵小蓉,林启美.微生物解磷的研究进展[J].土壤肥料,2001,5(3):7-11.
[6]青海省农牧厅.到2020年化肥使用量零增长行动方案[J].青海农技推广,2015, 20(2):3-6.
[7]AZZI V, KANSO A, KAZPARD V, et al. Lactuca sativa growth in compacted and non-compacted semi-arid alkaline soil under phosphate fertilizer treatment and cadmium contamination[J]. Soil Tillage Research, 2017, 165:1-10.
[8]MENDES G D 0, FREITAS A L M, PEREIRA 0 L, et al. Mechanisms of phosphate solubilization by fungal isolates when exposed to different P sources[J]. Annals of Microbiology, 2014, 64(1):239-249.
[9]胡滢滢,王璐,薛飞燕,等.餐饮废水发酵生产微生物菌肥及其应用的研究[J].环境工程,2017, 35(2):10-14.
[10]郭新愿,祁光霞,王永京,等.餐厨垃圾废水制备液态解磷菌剂研究[J].中国环境科学,2016, 36(11):3422-3428.
[11]BISWAS J K, BANERJEE A, RAI M, et al. Potential application of selected metal resistant phosphate solubilizing bacteria isolated from the gut of earthworm(Metaphire posthuma)in plant growth promotion[J]. Geoderma, 2018, 330:117-124.
[12]刘泽平,王志刚,徐伟慧,等.水稻根际促生菌的筛选鉴定及促生能力分析[J].农业资源与环境学报,2018, 35(2):119-125.
[13]中华人民共和国环境保护部.土壤有效磷的测定碳酸氢钠浸提钼锑抗分光光度法:HJ 704-2014[S].北京:中国环境科学出版社,2014.
[14]银婷婷,王敬敬,柳影,等.高效解磷菌的筛选及其促生机制的初步研究[J].生物技术通报,2015, 31(12):234-242.
[15]刘盼,赵华,林广修,等.解磷菌的筛选及培养基成分对解磷能力的影响[J].天津科技大学学报,2015, 30(6):17-22.
[16]邵锴,邱业先,徐婧.高效溶磷菌的筛选、鉴定及其溶磷特性[J].江苏农业科学,2017, 45(8):253-257.
[17]李媛媛.基于全基因组序列系统分类Kosakonia属的细菌[D].杭州:浙江大学,2016.
[18]王攀,任连海,邱银权,等.利用餐厨垃圾湿热处理脱出液制备液态菌肥研究[J].环境工程,2017, 35(6):88-92.
[19]关怡,尹娣,杜茜,等.产纤维素酶菌株Trametes sp. LYW-1的分离鉴定及产酶条件分析[J].中国农业大学学报,2018,23(10):96-102.
[20]李铉军,张先,付长雪,等.利用大酱中的枯草芽孢杆菌提高人参中Rg3含量的研究[J].吉林农业,2018, 30(18):50-53.
[21]蒋欣梅,夏秀华,于锡宏,等.微生物解磷菌肥对大棚茄子生长及土壤有效磷利用的影响[J].浙江大学学报(理学版),2012, 39(6):685-688.
[22]LIU F P, LIU H Q, ZHOU H L, et al. Isolation and characterization of phosphate-solubilizing bacteria from betel nut(Areca catechu)and their effects on plant growth and phosphorus mobilization in tropical soils[J]. Biology and Fertility of Soils,2014, 50(6):927-937.
[23]任友花,王羿超,李娜,等.微生物肥料高效解磷菌筛选及解磷机理探究[J].江苏农业科学,2016, 44(12):537-540.
[24]RAJWAR J, CHANDRA R, SUYAL D C, et al. Comparative phosphate solubilizing efficiency of psychrotolerant Pseudomonas jesenii MP1 and Acinetobacter sp ST02 against chickpea for sustainable hill agriculture[J]. Biologia, 2018, 73(8):793-802.
[25]邢芳芳,徐文凤,宋涛,等.一株高效溶磷真菌的溶磷特性及其促生效果研究[J].华北农学报,2013, 28(S1):357-360.
[26]王莉晶,高晓蓉,孙嘉怡,等.土壤解磷微生物作用机理及解磷菌肥对作物生长的影响[J].安徽农业科学,2008, 36(14):5948-5950.
[27]章爱群,郭海如,崔雪梅,等.主成分分析磷、砷交互作用对水稻生长的影响[J].环境科学与技术,2017, 40(7):48-53.
[28]林英,司春灿,韩文华,等.解磷微生物研究进展[J].江西农业学报,2017, 29(2):99-403.
[29]FERNANDO W G D, NAKKEERAN S, ZHANG Y. PGPR:Biocontrol and Biofertilization[M]. Berlin:Springer Netherlands,2005:67-109.
[30]刘泽平,王志刚,徐伟慧,等.水稻根际促生菌的筛选鉴定及促生能力分析[J].农业资源与环境学报,2018, 35(2):119-125.