生长调节剂对芦竹侧枝夏季扦插成活率的影响
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摘要
芦竹为禾本科重要的生物能源植物。探讨了不同类型和浓度的植物生长调节剂和生根粉(剂)对芦竹侧枝夏季扦插成活率的影响。以芦竹带有腋芽的当年生侧枝为插穗,用不同浓度的植物生长调节剂吲哚丁酸(IBA)、萘乙酸(NAA)、吲哚乙酸(IAA)以及生根粉(剂)溶液对插穗浸泡2 h后再行扦插,以清水浸泡为对照,15 d后统计侧枝扦插的成活率。结果表明,与对照相比,不同类型和不同浓度的植物生长调节剂对芦竹侧枝扦插的成活率具有不同程度的促进作用,尤以0.8 mg/L的NAA的促进效果显著,成活率达50.00%。生根粉(剂)也能提高插穗的成活率,0.625 g/L生根粉处理的成活率为40.00%,1.25 g/L的强力生根剂的效果最好,成活率为43.33%。将优选的生长调节剂和生根粉(剂)组合配合后再处理插穗,其成活率反而普遍下降,成活率最高的组合(0.05 mg/L的IAA与5.0 g/L生根剂)也只有25.00%。因此芦竹夏季侧枝扦插时,宜选用0.8 mg/L的NAA或1.25 g/L的强力生根剂作浸泡液进行预处理,以有效提高扦插的成活率。
Giant reed( Arundo donax) is an important bio-energy crop belonging to the Poaceae family. The effects of different types and concentrations of growth regulators and plant rooting powder( agent) on the survival rate of cuttings from lateral branches of this plant were discussed in the present paper. The current-year lateral branches with axillary buds were used as cuttings,and dipped into solutions of IBA,NAA,IAA as well as rooting powder and agent at varied concentrations for 2 hours. Water was employed as the control check. Then the cuttings were planted into the cultivation matrix,and the survival rates were counted 15 d later. The results showed that the growth regulators in different types and concentrations could increase the survival rate of cuttings when compared to the control,in which NAA at 0. 8 mg/L exhibited the highest survival rate of 50. 00%,significantly higher than other treatments. Similarly,plant rooting powder and agent enhanced the survival rate also,and the rooting powder with a concentration of 0. 625 g/L resulted in a rate of 40. 00%,while the highest rate of 43. 33% was recorded in 1. 25 g/L of rooting agent. The growth regulators and rooting agent with optimal effects on cutting rooting were matched up to pre-treat the cuttings. However,the survival rate of the cuttings decreased obviously,with 25% as the highest value in combination of IAA at 0. 05 mg/L and rooting agent at 5. 0 g/L. Plant growth regulator NAA of 0. 8 mg/L or plant rooting agent of 1. 25 g/L were suggested to be the optimal solutions in the pretreatment of cuttings from lateral branches of giant reed in summer,which could effectively accelerate the cutting survival rate.
Giant reed( Arundo donax) is an important bio-energy crop belonging to the Poaceae family. The effects of different types and concentrations of growth regulators and plant rooting powder( agent) on the survival rate of cuttings from lateral branches of this plant were discussed in the present paper. The current-year lateral branches with axillary buds were used as cuttings,and dipped into solutions of IBA,NAA,IAA as well as rooting powder and agent at varied concentrations for 2 hours. Water was employed as the control check. Then the cuttings were planted into the cultivation matrix,and the survival rates were counted 15 d later. The results showed that the growth regulators in different types and concentrations could increase the survival rate of cuttings when compared to the control,in which NAA at 0. 8 mg/L exhibited the highest survival rate of 50. 00%,significantly higher than other treatments. Similarly,plant rooting powder and agent enhanced the survival rate also,and the rooting powder with a concentration of 0. 625 g/L resulted in a rate of 40. 00%,while the highest rate of 43. 33% was recorded in 1. 25 g/L of rooting agent. The growth regulators and rooting agent with optimal effects on cutting rooting were matched up to pre-treat the cuttings. However,the survival rate of the cuttings decreased obviously,with 25% as the highest value in combination of IAA at 0. 05 mg/L and rooting agent at 5. 0 g/L. Plant growth regulator NAA of 0. 8 mg/L or plant rooting agent of 1. 25 g/L were suggested to be the optimal solutions in the pretreatment of cuttings from lateral branches of giant reed in summer,which could effectively accelerate the cutting survival rate.
引文
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[14]陈岑,李伯林,莫海萍.芦竹组培快繁研究初探[J].南方园艺,2016,27(2):1-5.
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[17]陈惠瑜,吕军.北疆芦竹的扦插快繁技术[J].北方园艺,2013,37(15):171.
[18]唐华国.芦竹扦插育苗技术的研究[J].林业实用技术,2000,43(2):13-14.
[19]SCORDIA D,ZANETTI F,VARGA S S,et al.New insights into the propagation methods of switchgrass,miscanthus and giant reed[J].Bioenergy Research,2015,8:1480-1491.
[20]邢尚军,张建锋,宋玉民,等.芦竹在黄河三角洲地区的苗木繁育及造林技术[J].山东林业科技,2003,33(6):29-30.
[21]王福民,梁宇鹤,张建国,等.花叶芦竹秋季扦插育苗技术[J].林业实用技术,2014,8:37.
[22]CEOTTO E,CANDILO M D.Shoot cuttings propagation of giant reed(Arundo donax L.)in water and moist soil:The path forward?[J].Biomass and Bioenergy,2010,34:1614-1623.
[2]赵丽萍,许卉.芦竹在滨海盐碱地的开发应用及栽培技术[J].北方园艺,2007,31(7):164-165.
[3]EID E M,YOUSSEF M S G,SHALTOUT K H.Population characteristics of giant reed(Arundo donax L.)in cultivated and naturalized habitats[J].Aquatic Botany,2016,129:1-8.
[4]余醉,李建龙,李高扬.芦竹作为清洁生物质能源牧草开发的潜力分析[J].草业科学,2009,26(6):62-69.
[5]VERVERIS C,GEORGHIOU K,CHRISTO DO ULAKIS N,et al.Fiber dimensions,lignin and cellulo secontent of various plant materials and their suitability for paper production[J].Industrial Crops and Products,2004,19:245-254.
[6]朱志国,周守标.铜锌复合胁迫对芦竹生理生化特性、重金属富集和土壤酶活性的影响[J].水土保持学报,2014,28(1):276-280.
[7]韩志萍,王趁义.不同生态型芦竹对Cd、Hg、Pb、Cu的富集与分布[J].生态环境学报,2007,16(4):1092-1097.
[8]IDRIS S M,JONES P L,SALZMAN S A,et al.Evaluation of the giant reed(Arundo donax)in horizontal subsurface flow wetlands for the treatment of recirculating aquaculture system effluent[J].Environmental Science and Pollution Research,2012,19(4):1159-1170.
[9]张燕,覃耀冠,陆文科,等.纤维素类草本植物——芦竹生物学特性及其开发利用前景[C].2014年能源草产业发展战略暨学术研讨会论文集,2014:218-227.
[10]CORNO L,PILU R,ADANI F,et al.Arundo donax L.:a nonfood crop for bioenergy and bio-compound production[J].Biotechnology Advances,2014,32(8):1535-1549.
[11]SGROI F,FODER M,TRAPANI AMD,et al.Economic evaluation of biogas plant size utilizing giant reed[J].Renewable and Sustainable Energy Reviews,2015,49:403-409.
[12]MARIANI C,CABRINI R,DANIN A,et al.Origin,diffusion and reproduction of the giant reed(Arundo donax L.):a promising weedy energy crop[J].Annals of Applied Biology,2010,157(2):191-202.
[13]刘文竹,赵惠恩.芦竹高效快繁体系的建立[J].中国农学通报,2015,31(16):146-150.
[14]陈岑,李伯林,莫海萍.芦竹组培快繁研究初探[J].南方园艺,2016,27(2):1-5.
[15]冉隆贤,文仕知.芦竹快速繁殖技术研究[J].经济林研究,1998,16(2):13-15.
[16]PIGNA G,DHILLON T,DLUGOSZ E M,et al.Methods for suspension culture,protoplast extraction,and transformation of highbiomass yielding perennial grass Arundo donax[J].Biotechnology Journal,2016,11(12):1657-1666.
[17]陈惠瑜,吕军.北疆芦竹的扦插快繁技术[J].北方园艺,2013,37(15):171.
[18]唐华国.芦竹扦插育苗技术的研究[J].林业实用技术,2000,43(2):13-14.
[19]SCORDIA D,ZANETTI F,VARGA S S,et al.New insights into the propagation methods of switchgrass,miscanthus and giant reed[J].Bioenergy Research,2015,8:1480-1491.
[20]邢尚军,张建锋,宋玉民,等.芦竹在黄河三角洲地区的苗木繁育及造林技术[J].山东林业科技,2003,33(6):29-30.
[21]王福民,梁宇鹤,张建国,等.花叶芦竹秋季扦插育苗技术[J].林业实用技术,2014,8:37.
[22]CEOTTO E,CANDILO M D.Shoot cuttings propagation of giant reed(Arundo donax L.)in water and moist soil:The path forward?[J].Biomass and Bioenergy,2010,34:1614-1623.