摘要
目的回顾性分析冻融周期中选择D5或D6囊胚解冻移植的妊娠结局。方法接受人类辅助生殖技术治疗的患者,新鲜取卵周期中D5和D6形成的可利用囊胚以玻璃化冷冻方法分别进行冷冻保存;在冻融周期,以排卵后或加用黄体酮第5天为移植窗,将囊胚以玻璃化解冻方法解冻复苏培养2h后植入患者子宫。分析D5和D6囊胚解冻移植后着床率、临床妊娠率、活产率及流产率等的差异。结果 D5囊胚和D6囊胚复苏后的存活率(99.03%VS99.65%)无显著差异;D5冻融囊胚移植的临床妊娠率(70.59%)、种植率(58.46%)和活产率(59.2%)则明显高于D6冻融囊胚移植的临床妊娠率(53.99%)、种植率(39.71%)和活产率(40.1%),差异具有显著性(P<0.05);而D5囊胚移植后的流产率(16.4%)显著低于D6囊胚移植(25.5%)(P<0.05)。结论选择D5囊胚移植能够提高冻融周期临床妊娠率、种植率及活产率,并降低胎儿流产率。
Objective:To retrospectively analyze the pregnancy outcome of frozen-thawed cycles using D5 blastocyst versus D6 blastocyst. Methods:The blastocysts developed on D5 or D6 in the fresh cycles were cryopreserved by vitrification method. In the frozen-thawed cycles,when the endometrium of the patient reached ≥7 mm,blastocysts were thawed and transfered on Day5 after ovulation or progesterone supplement. Thawed embryos were cultured for 2 h and then transfered into the uterus of patients.Implantation rate,clinical pregnancy rate,live birth rate and abortion rate were compared between D5-blastocyst-transfer group and D6-blastocys-transfer group. Results:No significant difference in the survival rate was found between D5 blastocysts and D6 blastocysts(99. 03% VS 99.65%). In D5-blastocyst-transfer group,the clinical pregnancy rate(70.59%),implantation rate(58.46%)and live birth rate(59.2%)were significantly higher than those in D6-blastocys-transfer group,which were53.99%,39.71% and 40.1% respectively(P<0.05). The abortion rate,however,significantly decreased in D5-blastocysttransfer group comparing to that in D6-blastocys-transfer group(16.4% VS 25.5%,P<0.05). Conclusion:Elective transfer of D5 blastocyst can improve the pregnancy outcome of frozen-thawed cycles.
引文
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