松嫩盐碱草地星星草根围优势菌种对其耐盐碱性的影响
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摘要
以已鉴定的星星草(Puccinellia tenuiflora)根围优势菌种摩西管柄囊霉(Funneliformis mosseae,FM)和根内根生囊霉(Rhizophagus intraradices,RI)为研究对象。将FM与RI接种到星星草根围80d后,进行盐碱处理11d,确定了形成共生体系后对其进行侵染率、菌根依赖性测定以及部分生理指标和离子含量测定,以探究星星草根围优势菌种对其耐盐碱性的影响及2种菌剂作用的差异性。结果表明:接种的RI和FM均能够与星星草植株形成菌根结构,并且对星星草的侵染率均很高,分别达到了80.86%和85.91%,在进行盐碱胁迫时均表现出了较强的菌根依赖性。在同一处理条件下,AM真菌能够显著降低星星草植株内相对电导率和脯氨酸含量,提高星星草对N、P、K 3种离子的吸收和利用。
The identification of the Puccinellia tenuiflorarhizosphere dominant species Funineliformis mosseae and Rhizophagus intraradices were taken as the research object.In order to investigate the effect of the two dominant fungi species on the salt and alkali tolerance of Puccinellia tenuiflora as well as their different effects.Funneliformis mosseae and Rhizophagus intraradices were purchased and inoculated with the rhizosphere of Puccinellia tenuiflora for 80 days,and were treated with salt and alkali for 11 days.After the formation of a symbiotic system,the infection rate,mycorrhizal dependence,some physiological indexes and ion contents were determined.The results indicated that Funneliformis mosseae and Rhizophagus intraradices could both form symbiosis with Puccinellia tenuiflora,the infection rate of which was very high and reached to 85.91% and 80.86%,respectively,showing strong mycorrhizal dependence during salt and alkali stress.Under the same stress conditions,AM fungi could significantly reduce the relative conductivity,proline content in the Puccinellia tenuiflora,increase the absorption and utilization of N,P and K ions.
The identification of the Puccinellia tenuiflorarhizosphere dominant species Funineliformis mosseae and Rhizophagus intraradices were taken as the research object.In order to investigate the effect of the two dominant fungi species on the salt and alkali tolerance of Puccinellia tenuiflora as well as their different effects.Funneliformis mosseae and Rhizophagus intraradices were purchased and inoculated with the rhizosphere of Puccinellia tenuiflora for 80 days,and were treated with salt and alkali for 11 days.After the formation of a symbiotic system,the infection rate,mycorrhizal dependence,some physiological indexes and ion contents were determined.The results indicated that Funneliformis mosseae and Rhizophagus intraradices could both form symbiosis with Puccinellia tenuiflora,the infection rate of which was very high and reached to 85.91% and 80.86%,respectively,showing strong mycorrhizal dependence during salt and alkali stress.Under the same stress conditions,AM fungi could significantly reduce the relative conductivity,proline content in the Puccinellia tenuiflora,increase the absorption and utilization of N,P and K ions.
引文
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[2]马朋坤.丛枝菌根真菌对盐碱化湿地土壤上碱蓬生长的影响研究[D].呼和浩特:内蒙古大学,2016.
[3]贺学礼,刘媞,赵丽莉.接种丛枝菌根对不同施氮水平下黄芪生理特性和营养成分的影响[J].应用生态学报,2009,20(9):2118-2122.
[4]刘灵,廖红,王秀荣,等.磷有效性对大豆菌根侵染的调控及其与根构型、磷效率的关系[J].应用生态学报,2008,19(3):567-568.
[5]吴强盛.园艺植物丛枝菌根研究与应用[M].北京:科学出版社,2010.
[6]弓明钦,王凤珍,陈羽,等.西南桦对菌根的依赖性及其接种效应研究[J].林业科学研究,2000,13(1):8-14.
[7]PHILIPS J M,HAYMAN D S.Improved procedures for clearing and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J].Transactions of the British Mycological Society,1970,55:158-161.
[8]戴建军,王洪亮,程岩.测定植物样品全氮含量的两种方法比较[J].东北农业大学学报,2000,31(1):36-38.
[9]杜一平.现代仪器分析方法[M].上海:华东理工大学出版社,2008.
[10]唐明.菌根真菌提高植物耐盐性[M].北京:科学出版社,2010.
[11]张素娜.生物材料中脯氨酸的作用及其反应动力学的研究[D].石家庄:河北大学,2007.
[12]JONGHO S,BENJAMIN M,EMMA G,et al.Activation of symbiosis signaling by arbuscular mycorrhizal fungi in legumes and rice[J].The Plant Cell,2015,27(3):823-838.
[13]姚青,冯固,李晓林.不同作物对VA菌根真菌的依赖性差异[J].作物学报,2000,26(6):874-878.
[14]杨春雪,陈飞,岳英男,等.松嫩盐碱草地26种植物根围丛枝菌根真菌多样性特征[J].草业科学,2015,32(12):2008-2020.
[15]刘洪光.AM真菌提高枸杞耐盐性的机制研究[D].杨凌:西北农林科技大学,2016.
[16]JAHROMI F,AROCA R,PORCEL R,et al.Influence of salinity on the in vitro development of Glomus intraradices and on the in vivo physiological and molecular responses of mycorrhizal lettuce plants[J].Microbial Ecology,2008,55(1):45-53.
[17]郭绍霞,刘润进.丛枝菌根真菌Glomus mossea对盐胁迫下牡丹渗透调节的影响[J].植物生理学报,2010,46(10):1007-1013.
[18]ALIASGHARZADEH N,SALEH N,RASTIN H,et al.Alizadeh Occurrence of arbuscular mycorrhizal fungi in saline soils of the Tabriz Plain of Iran in relation to some physical and chemical properties of soil[J].Mycorrhizal,2001,11(3):119-122.
[19]GIRI B,MUKERJI K.Mycorrhizal inoculant alleviates salt stress in Sesbania aegyptiaca and Sesbania grandiflora under field conditions:Evidence for reduced sodium and improved magnesium uptake[J].Mycorrhiza,2004,14(5):307-312.
[20]EVELIN H,GIRI B,KAPOOR R.Contribution of glomus intraradices inoculation to nutrient acquisition and mitigation of ionic imbalance in NaCl-stressed Trigonella foenum-graecum[J].Mycorrhiza,2012,22(3):203-217.