苦瓜枯萎病研究进展
|
摘要
苦瓜是江西省蔬菜产业的重要种类之一,但其产量和品质受到枯萎病的严重影响。该病害是由尖孢镰刀菌侵染引起的一种土传维管束病害。目前,化学药剂防治是主要的防治方法,但化学防治易产生环境污染、农药抗性及农药残留等问题。笔者综述了苦瓜枯萎病的发病规律、抗性鉴定、致病机制、农业防治和生物防治等方面的研究进展,针对目前存在的问题提出进一步的研究方向,以期为苦瓜枯萎病抗性育种提供参考。
Bitter gourd(Momordica charantia L.) is one of the most important crops in Jiangxi province, however, their yield and quality has been seriously limited by infection of Fusarium wilt. This soil-borne vascular disease is difficult to control. It can be resolved by treatment with chemical fungicide, but the use of this approach to treatment may also produce environmental pollution. The occurrence regulation,pathogenesis mechanism, identification technology in bitter gourd, control methods of this disease are reviewed, and the future research is also prospected, which will contribute to breeding fusarium wilt-resistant bitter gourd.
Bitter gourd(Momordica charantia L.) is one of the most important crops in Jiangxi province, however, their yield and quality has been seriously limited by infection of Fusarium wilt. This soil-borne vascular disease is difficult to control. It can be resolved by treatment with chemical fungicide, but the use of this approach to treatment may also produce environmental pollution. The occurrence regulation,pathogenesis mechanism, identification technology in bitter gourd, control methods of this disease are reviewed, and the future research is also prospected, which will contribute to breeding fusarium wilt-resistant bitter gourd.
引文
[1]SUN S K,HUANG J W.A new Fusarium wilt of birrer gourd in Taiwan[J].Plant Disease,1983,67(2):226-227.
[2]朱天圣,戚佩坤.苦瓜枯萎病病原菌研究[J].华南农业大学学报,1998,19(4):14-18.
[3]饶雪琴,李人柯.苦瓜枯萎病菌滤液鉴定苗期抗病性的初步探讨[J].江西农业大学学报,1999,21(3):367-369.
[4]张中义,冷怀琼,张志铭,等.植物病原菌学[M].成都:四川科学技术出版社,1986:123-140.
[5]陈振东.苦瓜枯萎病菌的鉴定、遗传多样性及其与寄主互作差异蛋白研究[D].南宁:广西大学,2014.
[6]杨叶.苦瓜枯萎病病菌的分离与鉴定[J].浙江农业学报,2010,22(3):354-357.
[7]KING B C,WAXMAN K D,NENNI N V,etal.Arsenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi[J].Biotechnology for Biofuels,2012,4(1):4.
[8]BANI M,RISPAL N,EVIDENTE A,etal.Identification of the main toxins isolated from Fusarium oxysporum f.sp.pisi race2 and their relation with isolates pathpgenicity[J].Journal of Agricultural and Food Chemistry,2014,62(12):2574-2580.
[9]MA L J,VAN DER DOES H C,BORKOVICH K A,etal.Comparative genomics reveals mobile pathopgenicity chromosomes in Fusarium[J].Nature,2010,464:367-373.
[10]OUMOULOUD A,OTMANI M E,ROUHOU H C,etal.Breeding melon for resistance to Fusarium wilt:recent developments[J].Euphytica,2013,192(2):155-169.
[11]KIKOT G E,HOURS R A,ALCONADA T M.Contribution of cell wall degrading enzymes to pathogensis of Fusarium graminearum:a review[J].Journal of Basic Microbiology,2009,49(3):231-240.
[12]ZVIRIN T,HERMAN R,BROTMAN Y,etal.Differential colonization and defence responses of resistant and susceptible melon lines infected by Fusarium oxysporum race 1.2[J].Plant Pathology,2010,59(12):576-585.
[13]RAMOS B,LóPEZ G,MOLINA A.Development of a Fusarium oxysporum f.sp.melonis functional GFP fluorescence tool to assist melon resistance breeding programmes[J].Plant Pathology,2015,64(6):1349-1357.
[14]GUPTA S,CHAKRABORTI D,SENGUPTA A,etal.Primary metabolism of chickpea is the initial target of wound inducing early sensed Fusarium oxysporum f.sp.ciceri race I[J].Plos One,2010,5(2):e9030.
[15]翁祖信,蒋兴祥,肖小文.黄瓜枯萎病抗病性鉴定方法研究—胚根接种法[J].中国蔬菜,1985(2):30-33.
[16]赵艳茹,杨德岐,陈春秀.西瓜枯萎病抗性品系鉴定与筛选[J].北京农业科学,1999,13(4):25-28.
[17]吴学宏,郝京京,王红梅,等.西瓜枯萎病菌几种接种方法比较实验[J].长江蔬菜,2003(3):38-39.
[18]关峰,万新建,张景云,等.江西省苦瓜枯萎病菌的分离、鉴定及苦瓜种植抗性的评价[J].江西农业大学学报,2017,39(3):476-484.
[19]罗方芳,何自福,佘小漫.广东苦瓜主要品种对枯萎病的抗性评价[J].广东农业科学,2010(9):72-73.
[20]曾华兰,何炼,刘朝辉,等.苦瓜品种资源抗枯萎病性评价[J].西南农业学报,2011,24(1):137-139.
[21]陈振东,黄如葵,黎起秦,等.苦瓜种质资源苗期枯萎病抗性鉴定[J].南方农业学报,2014,45(10):1776-1780.
[22]赵秀娟,唐鑫,胡开林.苦瓜枯萎病抗性鉴定与抗性遗传规律研究[J].园艺学报,2013,40(4):685-692.
[23]龙卫平,李育军,卢文佳,等.国外引进野生苦瓜资源的性状调查研究[J].长江蔬菜,2009(14):29-30.
[24]万新建,缪南生,胡新龙,等.早熟苦瓜新品种“赣优1号”、“赣优2号”的选育[J].江西农业,2007,19(4):37-39.
[25]郑师章,吴千红,王海波,等.普通生态学[M].上海:复旦大学出版社,1993.
[26]吴凤芝,周新刚.不同作物间作对黄瓜病害及土壤微生物群落多样性的影响[J].土壤学报,2009,46(5):899-906.
[27]刘阳华,肖昌华,刘志华.苦瓜枯萎病病残体上病菌的存活力及其传病作用[J].植物保护,2009,35(3):133-136.
[28]冯诚诚,黄如葵,黄熊娟,等.广西苦瓜-叶菜-豇豆-水稻一年四茬栽培模式[J].中国蔬菜,2016(1):98-100.
[29]WU H S,GAO Z Q,ZHOU X D,etal.Microbial dynamics and natural remediation patterns of Fusarium-infested watermelon soil under 3-yr of continuous falloe condition[J].Soil Use Manage,2013,29:220-229.
[30]KEINATH A P,HASSELL R L.Suppression of Fusarium wilt caused by Fusarium oxysporum f.sp.niceum race 2 on grafted triploid watermelon[J].Plant Disease.2014,98:1326-1332.
[31]张玉灿,赖正锋,张少平,等.丝瓜砧木对夏秋连作苦瓜产量及品质影响[J].中国农学通报,2013,29(4):189-194.
[32]唐锷.苦瓜枯萎病的发生及综合防治措施[J].科学种养,2009(6):30.
[33]陈瑶瑶,张双照,严良文,等.苦瓜几种主要病害及其防治措施[J].南方农业,2011,5(11):23-24.
[34]孙宝堂.苦瓜三种土传病害的诊断及防治[J].农家科技,2007(5):17.
[35]解开治,徐培智,张仁陟,等.养蛆产物对苦瓜枯萎病抑菌抗病的效果初探[J].农业环境科学学报,2007,26(b03):232-235.
[36]MARTYN R D,HARTZ T K.Use of soil solarization to control Fusarium wilt of watermelon[J].Plant disease,1986,70(8):762-766.
[37]曹群,丁文娟,赵兰凤,等.生物有机肥对冬瓜枯萎病及土壤微生物和酶活性的影响[J].华南农业大学学报,2015,36(2):35-42.
[38]王卿,林玲.瓜类枯萎病研究进展[J].中国瓜菜,2016,29(3):1-6.
[39]韩雪,潘凯,吴凤芝.不同抗性黄瓜品种根系分泌物对枯萎病病原菌的影响[J].中国蔬菜,2006(5):13-15.
[40]髙芬,马利平,乔雄梧,等.蜡质芽孢杆菌BC98-I发酵液与抑菌粗提取物对黄瓜枯萎病菌的抑菌特性研究[J].中国生态农业学报,2006,14(1):189-192.
[41]高克祥,刘晓光,郭润芳.木霉菌对杨树树皮泡溃疡病菌的拮抗作用的研究[J].林业科学,2001,37(5):82-86.
[42]袁飞,张春兰,沈其荣.酚酸物质减轻黄瓜枯萎病的效果及其原因分析[J].中国农业科学,2004,37(4):545-551.
[43]柳春燕,郭敏,林学政,等.拟康氏木霉和枯草芽孢杆菌对黄瓜枯萎病的协同防治作用[J].中国生物防治,2005,21(3):206-208.
[44]苗则彦,吕国忠,赵奎华.黄瓜内生细菌E1~E4防治黄瓜枯萎病研究[J].中国生态农业学报,2006,14(4):261-262.
[45]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系此生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[46]刘芸,朱育菁,陈清西.灰黄霉素对尖孢镰刀菌抑制作用的研究[J].中国农学通报2011,27(27):282-287.
[47]ZHAO S,LIU D Y,LING N,etal.Bioorganic fertilizer application significantly reduces the Fusarium oxysporum population and alters the composition of fungi communities of watermelon Fusarium wilt rhizosphere soil[J].Biology and Fertility of Soils,2014,50:765-774.
[48]胡云广,柴海云,崔堂兵.菌株BS-3的鉴定及其真菌抑制活性的初步研究[J].现代食品科技,2012,28(2):148-151.
[49]陈成,崔堂兵,于平儒.一株抗真菌的解淀粉芽孢杆菌的鉴定及其抗菌性研究[J].现代食品科技,2011,27(1):36-39.
[50]梁银,张谷月,王辰,等.一株拮抗放线菌的鉴定及其对黄瓜枯萎病的生防效应研究[J].土壤学报,2013,50(4):810-817.
[2]朱天圣,戚佩坤.苦瓜枯萎病病原菌研究[J].华南农业大学学报,1998,19(4):14-18.
[3]饶雪琴,李人柯.苦瓜枯萎病菌滤液鉴定苗期抗病性的初步探讨[J].江西农业大学学报,1999,21(3):367-369.
[4]张中义,冷怀琼,张志铭,等.植物病原菌学[M].成都:四川科学技术出版社,1986:123-140.
[5]陈振东.苦瓜枯萎病菌的鉴定、遗传多样性及其与寄主互作差异蛋白研究[D].南宁:广西大学,2014.
[6]杨叶.苦瓜枯萎病病菌的分离与鉴定[J].浙江农业学报,2010,22(3):354-357.
[7]KING B C,WAXMAN K D,NENNI N V,etal.Arsenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi[J].Biotechnology for Biofuels,2012,4(1):4.
[8]BANI M,RISPAL N,EVIDENTE A,etal.Identification of the main toxins isolated from Fusarium oxysporum f.sp.pisi race2 and their relation with isolates pathpgenicity[J].Journal of Agricultural and Food Chemistry,2014,62(12):2574-2580.
[9]MA L J,VAN DER DOES H C,BORKOVICH K A,etal.Comparative genomics reveals mobile pathopgenicity chromosomes in Fusarium[J].Nature,2010,464:367-373.
[10]OUMOULOUD A,OTMANI M E,ROUHOU H C,etal.Breeding melon for resistance to Fusarium wilt:recent developments[J].Euphytica,2013,192(2):155-169.
[11]KIKOT G E,HOURS R A,ALCONADA T M.Contribution of cell wall degrading enzymes to pathogensis of Fusarium graminearum:a review[J].Journal of Basic Microbiology,2009,49(3):231-240.
[12]ZVIRIN T,HERMAN R,BROTMAN Y,etal.Differential colonization and defence responses of resistant and susceptible melon lines infected by Fusarium oxysporum race 1.2[J].Plant Pathology,2010,59(12):576-585.
[13]RAMOS B,LóPEZ G,MOLINA A.Development of a Fusarium oxysporum f.sp.melonis functional GFP fluorescence tool to assist melon resistance breeding programmes[J].Plant Pathology,2015,64(6):1349-1357.
[14]GUPTA S,CHAKRABORTI D,SENGUPTA A,etal.Primary metabolism of chickpea is the initial target of wound inducing early sensed Fusarium oxysporum f.sp.ciceri race I[J].Plos One,2010,5(2):e9030.
[15]翁祖信,蒋兴祥,肖小文.黄瓜枯萎病抗病性鉴定方法研究—胚根接种法[J].中国蔬菜,1985(2):30-33.
[16]赵艳茹,杨德岐,陈春秀.西瓜枯萎病抗性品系鉴定与筛选[J].北京农业科学,1999,13(4):25-28.
[17]吴学宏,郝京京,王红梅,等.西瓜枯萎病菌几种接种方法比较实验[J].长江蔬菜,2003(3):38-39.
[18]关峰,万新建,张景云,等.江西省苦瓜枯萎病菌的分离、鉴定及苦瓜种植抗性的评价[J].江西农业大学学报,2017,39(3):476-484.
[19]罗方芳,何自福,佘小漫.广东苦瓜主要品种对枯萎病的抗性评价[J].广东农业科学,2010(9):72-73.
[20]曾华兰,何炼,刘朝辉,等.苦瓜品种资源抗枯萎病性评价[J].西南农业学报,2011,24(1):137-139.
[21]陈振东,黄如葵,黎起秦,等.苦瓜种质资源苗期枯萎病抗性鉴定[J].南方农业学报,2014,45(10):1776-1780.
[22]赵秀娟,唐鑫,胡开林.苦瓜枯萎病抗性鉴定与抗性遗传规律研究[J].园艺学报,2013,40(4):685-692.
[23]龙卫平,李育军,卢文佳,等.国外引进野生苦瓜资源的性状调查研究[J].长江蔬菜,2009(14):29-30.
[24]万新建,缪南生,胡新龙,等.早熟苦瓜新品种“赣优1号”、“赣优2号”的选育[J].江西农业,2007,19(4):37-39.
[25]郑师章,吴千红,王海波,等.普通生态学[M].上海:复旦大学出版社,1993.
[26]吴凤芝,周新刚.不同作物间作对黄瓜病害及土壤微生物群落多样性的影响[J].土壤学报,2009,46(5):899-906.
[27]刘阳华,肖昌华,刘志华.苦瓜枯萎病病残体上病菌的存活力及其传病作用[J].植物保护,2009,35(3):133-136.
[28]冯诚诚,黄如葵,黄熊娟,等.广西苦瓜-叶菜-豇豆-水稻一年四茬栽培模式[J].中国蔬菜,2016(1):98-100.
[29]WU H S,GAO Z Q,ZHOU X D,etal.Microbial dynamics and natural remediation patterns of Fusarium-infested watermelon soil under 3-yr of continuous falloe condition[J].Soil Use Manage,2013,29:220-229.
[30]KEINATH A P,HASSELL R L.Suppression of Fusarium wilt caused by Fusarium oxysporum f.sp.niceum race 2 on grafted triploid watermelon[J].Plant Disease.2014,98:1326-1332.
[31]张玉灿,赖正锋,张少平,等.丝瓜砧木对夏秋连作苦瓜产量及品质影响[J].中国农学通报,2013,29(4):189-194.
[32]唐锷.苦瓜枯萎病的发生及综合防治措施[J].科学种养,2009(6):30.
[33]陈瑶瑶,张双照,严良文,等.苦瓜几种主要病害及其防治措施[J].南方农业,2011,5(11):23-24.
[34]孙宝堂.苦瓜三种土传病害的诊断及防治[J].农家科技,2007(5):17.
[35]解开治,徐培智,张仁陟,等.养蛆产物对苦瓜枯萎病抑菌抗病的效果初探[J].农业环境科学学报,2007,26(b03):232-235.
[36]MARTYN R D,HARTZ T K.Use of soil solarization to control Fusarium wilt of watermelon[J].Plant disease,1986,70(8):762-766.
[37]曹群,丁文娟,赵兰凤,等.生物有机肥对冬瓜枯萎病及土壤微生物和酶活性的影响[J].华南农业大学学报,2015,36(2):35-42.
[38]王卿,林玲.瓜类枯萎病研究进展[J].中国瓜菜,2016,29(3):1-6.
[39]韩雪,潘凯,吴凤芝.不同抗性黄瓜品种根系分泌物对枯萎病病原菌的影响[J].中国蔬菜,2006(5):13-15.
[40]髙芬,马利平,乔雄梧,等.蜡质芽孢杆菌BC98-I发酵液与抑菌粗提取物对黄瓜枯萎病菌的抑菌特性研究[J].中国生态农业学报,2006,14(1):189-192.
[41]高克祥,刘晓光,郭润芳.木霉菌对杨树树皮泡溃疡病菌的拮抗作用的研究[J].林业科学,2001,37(5):82-86.
[42]袁飞,张春兰,沈其荣.酚酸物质减轻黄瓜枯萎病的效果及其原因分析[J].中国农业科学,2004,37(4):545-551.
[43]柳春燕,郭敏,林学政,等.拟康氏木霉和枯草芽孢杆菌对黄瓜枯萎病的协同防治作用[J].中国生物防治,2005,21(3):206-208.
[44]苗则彦,吕国忠,赵奎华.黄瓜内生细菌E1~E4防治黄瓜枯萎病研究[J].中国生态农业学报,2006,14(4):261-262.
[45]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系此生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[46]刘芸,朱育菁,陈清西.灰黄霉素对尖孢镰刀菌抑制作用的研究[J].中国农学通报2011,27(27):282-287.
[47]ZHAO S,LIU D Y,LING N,etal.Bioorganic fertilizer application significantly reduces the Fusarium oxysporum population and alters the composition of fungi communities of watermelon Fusarium wilt rhizosphere soil[J].Biology and Fertility of Soils,2014,50:765-774.
[48]胡云广,柴海云,崔堂兵.菌株BS-3的鉴定及其真菌抑制活性的初步研究[J].现代食品科技,2012,28(2):148-151.
[49]陈成,崔堂兵,于平儒.一株抗真菌的解淀粉芽孢杆菌的鉴定及其抗菌性研究[J].现代食品科技,2011,27(1):36-39.
[50]梁银,张谷月,王辰,等.一株拮抗放线菌的鉴定及其对黄瓜枯萎病的生防效应研究[J].土壤学报,2013,50(4):810-817.