哈茨木霉对设施草莓灰霉病的防治效果
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摘要
以草莓品种"红颜"为试材,通过田间试验研究了3×10~8 CFU·g~(-1)哈茨木霉菌可湿性粉剂对草莓灰霉病的防治效果。结果表明:3×108 CFU·g~(-1)哈茨木霉菌可湿性粉剂每667m~2施药量100~150g,对设施种植草莓灰霉病的防效最高可达75%左右。哈茨木霉可以用于防治草莓灰霉病,在草莓坐果期开始施药,每667m~2每次施药量100g,可取得较好的防治病害效果。
The strawberry cultivar ‘Hongyan’ was used as test material,the control effect of Trichoderma harzianum 300 million CFU·g~(-1) WP on grey mould in greenhouse grown strawberry by field experiments were investigate.The results showed that when T.harzianum300 million CFU·g~(-1) WP was applied at a dose of 100-150 g per 667 m~2,the control effect on the grey mould of strawberry in greenhouse could reached to 75%.T.harzianum could be used to control strawberry grey mould which applied each dose for 100 g per 667 m~2 at beginning of strawberry fruiting period,could achieve better control effects of disease.
The strawberry cultivar ‘Hongyan’ was used as test material,the control effect of Trichoderma harzianum 300 million CFU·g~(-1) WP on grey mould in greenhouse grown strawberry by field experiments were investigate.The results showed that when T.harzianum300 million CFU·g~(-1) WP was applied at a dose of 100-150 g per 667 m~2,the control effect on the grey mould of strawberry in greenhouse could reached to 75%.T.harzianum could be used to control strawberry grey mould which applied each dose for 100 g per 667 m~2 at beginning of strawberry fruiting period,could achieve better control effects of disease.
引文
[1]LAVY-MEIR G,BARLKAII-GOLAN R,KOPELIOEITCH E.Initiation at the flowering stage of postharvest Botrytis stemend rot in normal and non-ripening tomato fruits[J].Annals of Applied Biology,1988,112(3):393-396.
[2]EGASHIRA H,KUWASHIMA A,ISHIGURO H,et al.Screening of wild accessions resistant of grey mould(Botrytis cinerea Pers.)in Iycopersicon[J].Acta Physiologiae Plantarum,2000,22(3):324-326.
[3]PAPPAS A C.Epidemiology and control of Botrytis cinerea in tomato crops grown under cover in Greece[J].Bulletin OEPP/EPPO Bulletin,2000,30(2):269-274.
[4]BRAUN P G,SUTTON J C.Inoculum sources of Botrytis cinereain fruit rot of strawberries in Ontario[J].Canadian Journal of Plant Pathology,1987,9(1):1-5.
[5]韩永超,曾祥国,向发云,等.草莓花瓣脱落对果实灰霉病的影响[J].中国农业科学,2015,48(22):4460-4468.
[6]肖长坤,高苇,夏冰,等.设施栽培草莓灰霉病发生规律及其综合防治[J].中国植保导刊,2012,32(9):24-26.
[7]BOFF P,KASTELEIN P,de KRAKER J,et al.Epidemiology of grey mould in annual waiting-bed production of strawberry[J].European Journal of Plant Pathology,2001,107(6):615-624.
[8]张亚,王凌宇,刘双清,等.湖南省草莓灰霉病的发生与防治[J].微生物学杂志,2015,35(1):79-84.
[9]张建人,陆宏.南方草莓灰霉病的发生与综合防治[J].植物保护,1991,17(4):6-7.
[10]洪海林,李国庆,沈成艳,等.不同栽培方式下草莓灰霉病的发生动态[J].湖北农业科学,2016,55(13):3359-3363.
[11]高翠珠,杨红玲,黄夏宇骐,等.湖北省设施草莓灰霉病发生规律及流行因子分析[J].中国农业科学,2017,50(9):1617-1623.
[12]杨肖芳,童英富,苗立祥,等.几种不同药剂对草莓灰霉病防治效果比较分析[J].浙江农业科学,2016,57(3):367-369.
[13]谢昀烨,王连平,方丽,等.4种杀菌剂对浙江省不同地区的草莓灰霉病敏感性比较[J].农药,2017,56(2):135-137.
[14]李成,朱吉明,徐锦瑾,等.青浦区防治草莓灰霉病药剂筛选试验[J].上海农业科技,2017(4):123-124.
[15]吉沐祥,吴祥,束兆林,等.两种植物源复配杀菌剂对草莓灰霉病的防治效果初报[J].江西农业学报,2009,21(9):91-92.
[16]李涛,李成,吴丹,等.不同生物药剂对草莓灰霉病防效试验初报[J].上海农业科技,2017(4):126-127.
[17]刘连妹,钱雯霞,屈海泳.哈茨木霉孢子悬浮液对番茄幼苗生长及抗氧化酶活性的影响[J].江苏农业科学,2007,1(4):96-98.
[18]魏林,梁志怀,曹福祥,等.哈茨木霉T2-16发酵产物对豇豆种子细胞膜及细胞保护酶系统的影响[J].种子,2011,30(3):47-49.
[19]YEDIDIA I,BENHAMOU N,CHET I.Induction of defense responses in cucumber plants(Cucumis sativus L.)by the biocontrol agent Trichoderma harzianum[J].Applied and Environmental Microbiology,1999,65(3):1061-1070.
[20]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[21]姚彬,王傲雪,李景富.哈茨木霉对4种番茄病原真菌抑制作用的研究[J].东北农业大学学报,2009,40(5):26-31.
[22]管怀骥,陈莉.哈茨木霉TH-1菌株对小麦纹枯病的控制效果研究[J].安徽农业科学,2011,39(16):9664-9665.
[23]刘爱荣,陈双臣,陈凯,等.哈茨木霉对黄瓜尖孢镰刀菌的抑制作用和抗性相关基因表达[J].植物保护学报,2010,37(3):545-551.
[24]韩勇军.哈茨木霉对禾谷镰孢病原菌的抑菌活性研究[J].湖北农业科学,2010,49(2):356-358.
[25]王革,李天福,孙超岷,等.烟草木霉菌剂的肥效测定及叶面、根部定殖研究[J].中国烟草学报,2002,8(4):27-33.
[26]ZHANG F G,ZHU Z,WANG B B,et al.Optimization of Trichoderma harzianum T-E5biomass and determining the degradation sequence of biopolymers by FTIR in solid-state fermentation[J].Industrial Crops and Products,2013,49(8):619-627.
[27]黄亚丽,王淑霞,杜晓哲,等.一株具有诱导抗性木霉菌株的筛选及其对黄瓜灰霉病诱导抗性的初步研究[J].植物保护,2013,39(1):38-43.
[2]EGASHIRA H,KUWASHIMA A,ISHIGURO H,et al.Screening of wild accessions resistant of grey mould(Botrytis cinerea Pers.)in Iycopersicon[J].Acta Physiologiae Plantarum,2000,22(3):324-326.
[3]PAPPAS A C.Epidemiology and control of Botrytis cinerea in tomato crops grown under cover in Greece[J].Bulletin OEPP/EPPO Bulletin,2000,30(2):269-274.
[4]BRAUN P G,SUTTON J C.Inoculum sources of Botrytis cinereain fruit rot of strawberries in Ontario[J].Canadian Journal of Plant Pathology,1987,9(1):1-5.
[5]韩永超,曾祥国,向发云,等.草莓花瓣脱落对果实灰霉病的影响[J].中国农业科学,2015,48(22):4460-4468.
[6]肖长坤,高苇,夏冰,等.设施栽培草莓灰霉病发生规律及其综合防治[J].中国植保导刊,2012,32(9):24-26.
[7]BOFF P,KASTELEIN P,de KRAKER J,et al.Epidemiology of grey mould in annual waiting-bed production of strawberry[J].European Journal of Plant Pathology,2001,107(6):615-624.
[8]张亚,王凌宇,刘双清,等.湖南省草莓灰霉病的发生与防治[J].微生物学杂志,2015,35(1):79-84.
[9]张建人,陆宏.南方草莓灰霉病的发生与综合防治[J].植物保护,1991,17(4):6-7.
[10]洪海林,李国庆,沈成艳,等.不同栽培方式下草莓灰霉病的发生动态[J].湖北农业科学,2016,55(13):3359-3363.
[11]高翠珠,杨红玲,黄夏宇骐,等.湖北省设施草莓灰霉病发生规律及流行因子分析[J].中国农业科学,2017,50(9):1617-1623.
[12]杨肖芳,童英富,苗立祥,等.几种不同药剂对草莓灰霉病防治效果比较分析[J].浙江农业科学,2016,57(3):367-369.
[13]谢昀烨,王连平,方丽,等.4种杀菌剂对浙江省不同地区的草莓灰霉病敏感性比较[J].农药,2017,56(2):135-137.
[14]李成,朱吉明,徐锦瑾,等.青浦区防治草莓灰霉病药剂筛选试验[J].上海农业科技,2017(4):123-124.
[15]吉沐祥,吴祥,束兆林,等.两种植物源复配杀菌剂对草莓灰霉病的防治效果初报[J].江西农业学报,2009,21(9):91-92.
[16]李涛,李成,吴丹,等.不同生物药剂对草莓灰霉病防效试验初报[J].上海农业科技,2017(4):126-127.
[17]刘连妹,钱雯霞,屈海泳.哈茨木霉孢子悬浮液对番茄幼苗生长及抗氧化酶活性的影响[J].江苏农业科学,2007,1(4):96-98.
[18]魏林,梁志怀,曹福祥,等.哈茨木霉T2-16发酵产物对豇豆种子细胞膜及细胞保护酶系统的影响[J].种子,2011,30(3):47-49.
[19]YEDIDIA I,BENHAMOU N,CHET I.Induction of defense responses in cucumber plants(Cucumis sativus L.)by the biocontrol agent Trichoderma harzianum[J].Applied and Environmental Microbiology,1999,65(3):1061-1070.
[20]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[21]姚彬,王傲雪,李景富.哈茨木霉对4种番茄病原真菌抑制作用的研究[J].东北农业大学学报,2009,40(5):26-31.
[22]管怀骥,陈莉.哈茨木霉TH-1菌株对小麦纹枯病的控制效果研究[J].安徽农业科学,2011,39(16):9664-9665.
[23]刘爱荣,陈双臣,陈凯,等.哈茨木霉对黄瓜尖孢镰刀菌的抑制作用和抗性相关基因表达[J].植物保护学报,2010,37(3):545-551.
[24]韩勇军.哈茨木霉对禾谷镰孢病原菌的抑菌活性研究[J].湖北农业科学,2010,49(2):356-358.
[25]王革,李天福,孙超岷,等.烟草木霉菌剂的肥效测定及叶面、根部定殖研究[J].中国烟草学报,2002,8(4):27-33.
[26]ZHANG F G,ZHU Z,WANG B B,et al.Optimization of Trichoderma harzianum T-E5biomass and determining the degradation sequence of biopolymers by FTIR in solid-state fermentation[J].Industrial Crops and Products,2013,49(8):619-627.
[27]黄亚丽,王淑霞,杜晓哲,等.一株具有诱导抗性木霉菌株的筛选及其对黄瓜灰霉病诱导抗性的初步研究[J].植物保护,2013,39(1):38-43.